Fermentation method of alternaria tenuissima
A technology of Alternaria tennatus and fermentation method, which is applied in the field of herbicide development, and can solve the problems of slow control effect, hindering the pace of registration and development of biological herbicides, and inability to store biological herbicides for a long time
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Embodiment 1
[0034] Step 1, the activation of strains:
[0035] Take out the HZ-1 strains stored at 4°C, pick some colonies and transfer them to fresh PDA medium, culture them at 25°C for 7 days, then pick some colonies from this medium and transfer them to fresh PDA plates, and repeat this for 2 ~3 times, the HZ-1 strain with vigorous growth after activation was obtained;
[0036] Among them, the HZ-1 strain is Alternaria tenuissima, which was provided by the Comprehensive Pest Control Laboratory of the Plant Protection Institute of Qinghai Academy of Agricultural Sciences (preserved on the inclined plane of the test tube in the refrigerator at 4°C); it was stored on January 11, 2016. It was deposited at the General Microbiology Center of China Committee for the Collection of Microorganisms, No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, China, and the preservation number is CGMCC No.11992.
[0037] Wherein, wherein, the carbon source of PDA medium is sucrose, and...
Embodiment 2
[0041] Step 1, the activation of strains:
[0042] Take out the HZ-1 strains stored at 4°C, pick some colonies and transfer them to fresh PDA medium, culture them at 25°C for 7 days, then pick some colonies from this medium and transfer them to fresh PDA plates, and repeat this for 2 ~3 times, the HZ-1 strain with vigorous growth after activation was obtained;
[0043] Among them, the HZ-1 strain is Alternaria tenuissima, which was provided by the Comprehensive Pest Control Laboratory of the Plant Protection Institute of Qinghai Academy of Agricultural Sciences (preserved on the inclined plane of the test tube in the refrigerator at 4°C); it was stored on January 11, 2016. It was deposited at the General Microbiology Center of China Committee for the Collection of Microorganisms, No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, China, and the preservation number is CGMCC No.11992.
[0044] Wherein wherein, the carbon source of PDA medium is sucrose, and ...
Embodiment 3
[0048] Step 1, the activation of strains:
[0049] Take out the HZ-1 strains stored at 4°C, pick some colonies and transfer them to fresh PDA medium, culture them at 25°C for 7 days, then pick some colonies from this medium and transfer them to fresh PDA plates, and repeat this for 2 ~3 times, the HZ-1 strain with vigorous growth after activation was obtained;
[0050] Among them, the HZ-1 strain is Alternaria tenuissima, which was provided by the Comprehensive Pest Control Laboratory of the Plant Protection Institute of Qinghai Academy of Agricultural Sciences (preserved on the inclined plane of the test tube in the refrigerator at 4°C); it was stored on January 11, 2016. It was deposited at the General Microbiology Center of China Committee for the Collection of Microorganisms, No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, China, and the preservation number is CGMCC No.11992.
[0051] Wherein, wherein, the carbon source of PDA medium is sucrose, and...
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