Fermentation method of alternaria tenuissima

A technology of Alternaria tennatus and fermentation method, which is applied in the field of herbicide development, and can solve the problems of slow control effect, hindering the pace of registration and development of biological herbicides, and inability to store biological herbicides for a long time

Inactive Publication Date: 2018-05-04
QINGHAI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microbial herbicides are easily affected by environmental and climatic factors, and their control effects are slow and unstable, which restricts their development. In addition, due to the physiological characteristics of microbial cells themselves, there is currently no effective solution to the technical bottleneck of long-term storage of biological cells at room temperature, making biological herbicides impossible. Long-term storage like chemical herbicides is at a disadvantage compared with chemical herbicides, which greatly hinders the pace of registration and development of biological herbicides

Method used

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  • Fermentation method of alternaria tenuissima
  • Fermentation method of alternaria tenuissima
  • Fermentation method of alternaria tenuissima

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Step 1, the activation of strains:

[0035] Take out the HZ-1 strains stored at 4°C, pick some colonies and transfer them to fresh PDA medium, culture them at 25°C for 7 days, then pick some colonies from this medium and transfer them to fresh PDA plates, and repeat this for 2 ~3 times, the HZ-1 strain with vigorous growth after activation was obtained;

[0036] Among them, the HZ-1 strain is Alternaria tenuissima, which was provided by the Comprehensive Pest Control Laboratory of the Plant Protection Institute of Qinghai Academy of Agricultural Sciences (preserved on the inclined plane of the test tube in the refrigerator at 4°C); it was stored on January 11, 2016. It was deposited at the General Microbiology Center of China Committee for the Collection of Microorganisms, No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, China, and the preservation number is CGMCC No.11992.

[0037] Wherein, wherein, the carbon source of PDA medium is sucrose, and...

Embodiment 2

[0041] Step 1, the activation of strains:

[0042] Take out the HZ-1 strains stored at 4°C, pick some colonies and transfer them to fresh PDA medium, culture them at 25°C for 7 days, then pick some colonies from this medium and transfer them to fresh PDA plates, and repeat this for 2 ~3 times, the HZ-1 strain with vigorous growth after activation was obtained;

[0043] Among them, the HZ-1 strain is Alternaria tenuissima, which was provided by the Comprehensive Pest Control Laboratory of the Plant Protection Institute of Qinghai Academy of Agricultural Sciences (preserved on the inclined plane of the test tube in the refrigerator at 4°C); it was stored on January 11, 2016. It was deposited at the General Microbiology Center of China Committee for the Collection of Microorganisms, No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, China, and the preservation number is CGMCC No.11992.

[0044] Wherein wherein, the carbon source of PDA medium is sucrose, and ...

Embodiment 3

[0048] Step 1, the activation of strains:

[0049] Take out the HZ-1 strains stored at 4°C, pick some colonies and transfer them to fresh PDA medium, culture them at 25°C for 7 days, then pick some colonies from this medium and transfer them to fresh PDA plates, and repeat this for 2 ~3 times, the HZ-1 strain with vigorous growth after activation was obtained;

[0050] Among them, the HZ-1 strain is Alternaria tenuissima, which was provided by the Comprehensive Pest Control Laboratory of the Plant Protection Institute of Qinghai Academy of Agricultural Sciences (preserved on the inclined plane of the test tube in the refrigerator at 4°C); it was stored on January 11, 2016. It was deposited at the General Microbiology Center of China Committee for the Collection of Microorganisms, No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, China, and the preservation number is CGMCC No.11992.

[0051] Wherein, wherein, the carbon source of PDA medium is sucrose, and...

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Abstract

The invention discloses a Fermentation method of alternaria tenuissima. The fermentation method comprises the following steps: taking out an alternaria tenuissima culture HZ-1 stored at 4 DEG C; picking up part of bacterial colony and inoculating the same to a fresh PDA culture medium to be cultured, then picking up part of the bacterial colony from the culture medium and inoculating the same to afresh PDA culture medium, and repeating the step 2-3 times to obtain an activated HZ-1 culture which is vigorous in growing power; taking the HZ-1 culture cultured for 7d in a petri dish, perforating8mm bacterial cakes by a perforator, and inoculating the bacterial cakes 10/L into the PDA liquid culture medium; and culturing the cakes in an oscillator, and filtering the mixture to obtain a HZ-1fermenting liquid. According to the fermentation method, the culture HZ-1 is reproduced massively, thereby laying a theoretical foundation for industrial production and further popularization and application of a biological fungicide.

Description

technical field [0001] The invention belongs to the technical field of herbicide development, and in particular relates to a fermentation method of Alternaria ultrafines. Background technique [0002] Weeds refer to non-cultivated plants growing in farmland, which compete with crops for nutrients, sunlight, water, space, etc. Some weeds can promote the occurrence of crop diseases and insect pests, thereby reducing the yield and quality of crops. And some weed fruits and pollen contain toxins, which can cause human and animal poisoning. Therefore, the control of weeds has always been crucial in agricultural production, and the most widely used methods of weed control are artificial control and chemical control. Since humans began to engage in agricultural production, weeds have been removed manually or with simple agricultural tools. Although the control effect of this method is good, it is time-consuming and labor-intensive, and it cannot prevent a wide range of weeds in a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01P13/00C12R1/645
CPCC12N1/14
Inventor 朱海霞马永强程亮郭青云魏有海郭良芝
Owner QINGHAI UNIVERSITY
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