Bacillus cereus strain CZBC1 capable of dissolving pond oscillatoria, and its application

A technology of Bacillus cereus and pond oscillating algae, applied in the direction of bacteria, microorganism-based methods, water/sludge/sewage treatment, etc., can solve the research on whether Bacillus cereus strains can dissolve pond oscillating algae, ignore each other Contact, industrial technology application and development limitations, etc., to achieve the effect of inhibiting the formation of Oscillator algae, optimizing technical reserves, and obvious bacteriolysis effect

Active Publication Date: 2013-10-16
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some scholars have explored the algal phase regulation technology of microalgae in culture ponds (Corre, 2005; Cremen, 2007; Yusoff, 2002), and analyzed the physiological characteristics of relevant microalgae and the competition characteristics among algae (Kuwata, 2000; Klausmeier, 2004; Smith, 1983; Piazzi, 2002; Litchman, 2003), but often ignore the interrelationship among "environmental nutrition-microbes-microalgae", which is quite different from the actual pond environment, so , subject to certain restrictions in the development of industrial technology applications
Whether Bacillus cereus strains can dissolve pond Oscillatoria has not been studied

Method used

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  • Bacillus cereus strain CZBC1 capable of dissolving pond oscillatoria, and its application
  • Bacillus cereus strain CZBC1 capable of dissolving pond oscillatoria, and its application
  • Bacillus cereus strain CZBC1 capable of dissolving pond oscillatoria, and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The screening method of the Bacillus cereus bacterial strain CZBC1 of embodiment 1 dissolving Oscillator algae

[0018] 1. Material preparation

[0019] 1. Bacterial source

[0020] Obtained from a prawn farm in Honghai Bay, Shanwei;

[0021] 2. Medium

[0022] (1) Microalgae BG11 liquid medium;

[0023] (2) Add NH to sterilized pond water 4 Cl1.0~1.5g / L and Na 2 HPO 4 30~40mg / L;

[0024] (3) Microbial nutrient broth medium

[0025] 2. Isolation and screening of strains

[0026] In a farm in Dianbai, Maoming, the dominant Oscillator algae in the pond water body - Oscillatoria viridis was isolated and cultivated, and the culture medium can be two kinds, one is BG11 liquid medium, and the other is the sterilized pond water Add NaNO 3 1.0~1.5g / L and K 2 HPO 4 30 ~ 40mg / L; at the same time, the pond water dominated by Oscillator algae (the number of Oscillator algae accounts for more than 95% of the total microalgae) is cultivated, and no nutrients are added dur...

Embodiment 2

[0027] Example 2 Identification of Bacillus cereus Strain CZBC1 Dissolving Pond Oscillatoria

[0028] The present invention conducts physiological and biochemical identification and 16S rDNA molecule identification on the algae-dissolving algae-dissolving strain CZBC1 capable of dissolving Oscillator algae, and determines the species of the strain from the molecular level, combined with bacterial morphological characteristics and physiological and biochemical characteristics analysis. 16S rDNA sequence analysis mainly follows the following steps:

[0029] Extraction of bacterial genomic DNA:

[0030] 1. Use an autoclaved toothpick to pick a single colony and inoculate it in the expansion medium for overnight cultivation.

[0031] 2. Take 1.5mL of bacterial culture solution, centrifuge at 10,000rpm (11,500×g) for 1 minute, and suck up the supernatant as much as possible.

[0032] 3. Add 200 μL of buffer GA to the cell pellet, shake until the cells are completely suspended, ad...

Embodiment 3

[0070] Example 3 Small-scale Laboratory Application of Bacillus cereus Strain CZBC1 Dissolving Pond Oscillation Algae

[0071] Cultivate Bacillus cereus strain CZBC1 at 30°C and 200r / min for 16 hours, and add it to the pure culture algae liquid of Vibrella viridis, Chlorella pyrenoidosa, and Nitzschia crescentus. The initial density of the three microalgae 5.0×10 6 cell / mL, 1.6×10 6 cell / mL, 5.3×10 5 cell / mL, the number of bacteria added is 10 7cfu / mL. The bacteria and algae polyculture system was cultivated for 8 days under the conditions of temperature 25-28°C, light intensity 2500-4000 lx, and light-dark cycle 12h:12h. The results showed that all the vibrating algae died on the 5th day, and the algal cells were dissolved and broken; Chlorella pyrenoidosa and Nitzschia lunata grew slightly, and the algal cell densities were 1.7×10 7 cell / mL and 2.0×10 6 cell / mL. This shows that CZBC1 has a good dissolution effect on Oscillatoria, and has no adverse effects on fine gre...

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Abstract

The invention discloses a Bacillus cereus strain CZBC1 capable of dissolving pond oscillatoria. The strain is Bacillus cereus, is preserved in China Center for Type Culture Collection in Wuhan, China on April 9, 2013 as Bacillus cereus CZBC1, and has a preservation number of CCTCC NO:M2013130. The Bacillus cereus strain CZBC1 has obvious dissolving and eliminating effects on a plurality of dominant oscillatoria in culture water. The invention also discloses an application of the Bacillus cereus strain CZBC1 capable of dissolving pond oscillatoria in the dissolving of the pond oscillatoria.

Description

technical field [0001] The invention belongs to the technical field of using microorganisms to control microalgae, in particular to a Bacillus cereus strain CZBC1 for dissolving Oscillator algae in ponds and its application. Background technique [0002] Microalgae play a pivotal role in the material cycle and energy flow in the water environment. It is indispensable for maintaining the normal function of the water ecosystem, promoting the absorption and transformation of environmental nitrogen and phosphorus nutrients, and stabilizing the water environment (Li Zhuojia, 2005 ; Zhaohui, 1999), Kuang Qijun et al. (2001) used algae to purify water quality and achieved good results. Generally, there are 2 to 3 dominant species of microalgae in shrimp culture ponds, and the fewer dominant species, the higher the degree of dominance. Studies have shown that green algae and diatoms are generally the main species in the early stage of culture, and the number of microalgae increases ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/085
Inventor 曹煜成王丽花文国樑李卓佳胡晓娟
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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