Method for simultaneously purifying and detecting avian leukosis and avian salmonellosis by utilizing one hatching egg and application of method

A technology for chicken salmonellosis and avian leukemia, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve problems such as lack of effective unification, and achieve the effects of improving the utilization rate, reducing the number of sampling times, and improving the detection efficiency.

Inactive Publication Date: 2019-01-11
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the purification of avian leukemia and chicken salmonellosis i...

Method used

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  • Method for simultaneously purifying and detecting avian leukosis and avian salmonellosis by utilizing one hatching egg and application of method
  • Method for simultaneously purifying and detecting avian leukosis and avian salmonellosis by utilizing one hatching egg and application of method

Examples

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Effect test

Embodiment 1

[0021] A method for simultaneously purifying and detecting avian leukaemia and chicken salmonellosis by using one breeding egg comprises the following steps:

[0022] (1) Collection and numbering of breeding eggs: Breeding chickens to be purified are reared in single cages, and each breeding chicken (or cage position) is numbered; breeding eggs at a specific stage are collected as purification test samples, and breeding eggs are marked and compared with chickens. Only numbers correspond to each other;

[0023] (2) Sample treatment: In the laboratory, the surface of the eggs was disinfected with tincture of iodine and medical alcohol, and 1ml of egg white was placed in a 1.5ml PE tube with a sterilized micropipette, and 1ml of egg yolk was placed in another 1.5ml PE tube. Put them in PE tubes and mark them separately. After completion, put egg whites and egg yolks in a -20°C freezer to freeze and thaw for 1 to 3 times; take out the samples before use and return to room temperat...

Embodiment 2

[0028] Using the method described in Example 1, 30 breeding egg samples of a certain local breed chicken farm were detected, and the blood of the corresponding chickens was collected on the spot, and the whole blood plate agglutination test was used to detect the Salmonella antibody. As a control, the results were as follows:

[0029] Table 1 Results of simultaneous detection of ALV virus antigen and Salmonella antibody

[0030]

[0031]

[0032] Positive test numbers for avian leukemia and chicken salmonellosis statistics: positive test numbers for avian leukosis antigen: 07, 12, 14, 23; positive test numbers for chicken Salmonella antibody tests: 04, 12, 24, positive test numbers for control methods: 12, and all eliminated Positive number chickens: as in this example, 04, 07, 12, 14, 23, and 24 are all eliminated. This test shows that the yolk antibody detection method is more accurate than the whole blood detection method, and can effectively reduce false negative res...

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Abstract

The invention belongs to the field of animal husbandry and veterinary and in particular relates to a method for simultaneously purifying and detecting avian leukosis and avian salmonellosis by utilizing one hatching egg and application of the method. The method comprises the following steps: feeding breeder flocks in single cages and encoding; collecting primary hatching eggs of the breeder flocks; enabling the primary hatching eggs to correspond to numbers of the hatching eggs one by one; disinfecting the surfaces of the hatching eggs and opening egg shells from hatching egg air chamber ends;sucking egg white and yolks and putting into PE (Polyethylene) tubes respectively and marking; putting egg white and yolk samples into a refrigerator and freezing and thawing for a plurality of times; taking the frozen and thawed egg white; detecting an ALV virus antigen through an avian leukosis ELISA (Enzyme Linked Immunosorbent Assay) detection method; taking the frozen and thawed yolks and adding normal saline and chloroform; after uniformly mixing on an oscillator, centrifuging and layering a solution, wherein a lower layer is an organic solvent, a middle layer is denatured lipid proteinand an upper layer is an extracted antibody; and sucking the antibody of the upper layer and detecting a salmonellosis antibody through a plate agglutination method; and eliminating chicken flock individuals with positive detection results of the ALV virus antigen and the salmonellosis antibody.

Description

technical field [0001] The invention belongs to the field of animal husbandry and veterinary medicine, and specifically relates to a method for simultaneously purifying and detecting avian leukemia and chicken salmonellosis by using one breeding egg and its application. Background technique [0002] Avian leukemia is a contagious neoplastic disease caused by avian leukemia virus (ALV). First reported in 1908, the disease has occurred and spread in many countries so far. This case was first discovered in broiler flocks in my country. In recent years, cases of layer hens and local chicken flocks have also been reported continuously, and the incidence is on the rise. ALV belongs to the genus αretrovirus of the family Retroviridae, which belongs to the same retrovirus as human immunodeficiency virus (HIV), and has a similar viral genome structure. The virus can be divided into 11 subgroups from A to K, of which only subgroups A, B, C, D, J and K can infect chickens. Pathogeni...

Claims

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Application Information

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IPC IPC(8): G01N33/569
CPCG01N33/56916G01N33/56983
Inventor 汪宏才罗青平张腾飞
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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