41 results about "Halophilic bacteria" patented technology

The invention relates to a halophilic bacterium plasmid based on a sucrose lethal gene. The invention also provides a method for obtaining engineering halophilic bacteria without plasmids by using the halophilic bacteria plasmids.

The invention discloses a moisturizer prepared from a lysate extract produced by fermentation of bacillus subtilis. The hydroxydecyl ubiquinone is identified in Y-3FLE, and the yield of the hydroxydecyl ubiquinone is 0.45 mu g / g after the hydroxydecyl ubiquinone is cultured for 4 days, so that the quinone substances are identified in the Halophilic bacteria in the Yuncheng Salt Lake for the first time. The Y-3FLE is prepared by adopting absolute ethyl alcohol as a solvent, the oxidation resistance of the Y-3FLE is investigated through an in-vitro experiment, and a result shows that the Y-3FLE is relatively high in reducing capacity and relatively high in oxidation resistance. A human body closed patch test result shows that the Y-3FLE has no obvious irritation to human skin. The method is applied to the moisturizer, and human body efficacy evaluation is carried out through a multi-probe skin tester. Results show that the moisturizer added with 1% of Y-3FLE has multiple remarkable effects of moisturizing, repairing, nourishing and the like.

The invention discloses a method for preparing a carbon monoxide normal-temperature oxidation catalyst by using electrolytic manganese residues and oil sludge, which comprises the following steps: uniformly mixing the oil sludge and the electrolytic manganese residues to obtain manganese oil sludge, uniformly stirring the manganese oil sludge and mixed halophilic desulfurizing bacteria, fermenting, and drying to constant weight to obtain fermented manganese oil sludge, the method comprises the following steps: carbonizing dry fermented manganese oil sludge to obtain a manganese carbide oil material, uniformly mixing the manganese carbide oil material with a blueberry extract to obtain a manganese carbide oil extract mixture, uniformly mixing and stirring the obtained manganese carbide oil extract mixture with a chloroplatinic acid solution, standing, filtering, washing, and drying to obtain the mixed halophilic desulfurizing bacteria. Drying to constant weight to obtain a platinum-doped carbonization extraction precursor, and finally roasting the platinum-doped carbonization extraction precursor to obtain the carbon monoxide oxidation catalyst. The composite catalyst with high specific surface area and multiple active sites is formed, and resource utilization of waste is achieved.

The invention discloses a microbial material for reducing salt heave and frost heave of highway subgrade salinized soil as well as a preparation method and application of the microbial material, a microorganism is nitrogen-fixing bacteria and is classified and named as Neorhizobium alkalisoli XJIPC-GNJ0001, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on January 20, 2025, and the preservation number is CGMCC No.33310. The invention further discloses a preparation method of the microbial material for reducing the salt heave and frost heave of the highway subgrade salinized soil and application of the microbial material for reducing the salt heave and frost heave of the highway subgrade salinized soil. The strain belongs to extremely halophilic bacteria and adapts to a strong alkali environment, and a microbial material prepared from fermentation liquor of the strain can reduce salt heave and frost heave of highway subgrade salinized soil. The preparation method is simple, low in cost and easy to produce, and the material is green, environment-friendly and good in stability. The microbial solid preparation of the novel alkali rhizobium and the fermentation liquor can be used as a cementing substance and forming power, is used for preparing a material for preventing salt heaving and frost heaving of road salinized soil, can relieve or reduce engineering diseases such as salt heaving and frost heaving in road subgrade filling in a salinized soil area, is beneficial to road subgrade protection and stabilization, and has a good application prospect. The resource utilization way of the salinized soil is widened, and the application prospect is wide.

The invention discloses a saline-alkali soil raw water double-micro-flooding root-promoting technology. According to the method, 150-1500 kg of a soil conditioner, 30-150 kg of a soil remediation agent and 450-1500 kg of a soil biological conditioning fertilizer are added into per hectare of original soil according to the actual salinity condition of farmland soil. According to the actual condition of crop cultivation, ditching, ridging or bedding, drip irrigation tape laying, irrigation and seeding are carried out; and the halophilic bacteria agent is conveyed and applied through a drip irrigation belt after being uniformly stirred. By synergistically promoting metabolism of halophilic microorganisms to remove salt and improve rhizosphere microenvironment of crops, root growth is facilitated, saline-alkaline resistance of the crops is improved, and high yield and high quality of the crops are achieved. The technology does not need soil dressing, needs to consume a large amount of fresh water resources, does not have complex treatment engineering, and saves cost investment. The saline-alkali soil is improved gradually and effectively while the yield and economic benefits are obtained freely through saline-alkali soil planting, and a breakthrough positive contribution is made to planting crops on the saline-alkali soil.

The invention discloses application of a natural bacteriostatic agent in inhibition of salt-tolerant cladosporium in a tea beverage and the tea beverage, and relates to the technical field of food additives, and the natural bacteriostatic agent in the application comprises at least one of citral, smoked plum and ginger extract. In the technical scheme of the invention, any one of citral, smoked plum and ginger extract is independently added into the tea beverage obtained by a sterilization canning technology, so that the propagation of salt-tolerant cladosporium can be effectively inhibited; meanwhile, two or three of citral, smoked plum and ginger extract are added into the tea beverage to synergistically inhibit propagation of salt-tolerant cladosporium, so that the dosage of a natural bacteriostatic agent is reduced, and the taste of the tea beverage is improved.

This invention discloses an extremely salt-tolerant halophilic bacterium, HD16, which can grow under extreme salinity conditions up to 4.0 M sodium chloride (approximately 230 g / L sodium chloride) and also exhibits high alkalinity tolerance, surviving at pH 7–11. Therefore, it is predicted to have broad application prospects in wastewater treatment and other fields. Furthermore, it can be used to produce polyhydroxyalkali (PHA). When cultured in a 500 ml Erlenmeyer flask, the cell dry weight yield reaches 9.45 g / L, with PHA accounting for 72.97% of the bacterial dry weight. The extreme salt tolerance and high alkalinity tolerance of this strain enable it to efficiently accumulate polyhydroxyalkali esters under open, non-sterile conditions.

The invention relates to a recombinant halomonas, which comprises an ilvC (acetohydroxy acid isoreductase, AHAIR) gene, an ilvD (dihydroxy acid dehydratase, DHAD) gene and an optional ilvE (transaminase, TA) gene, wherein the ilvC (acetohydroxy acid isoreductase, AHAIR) gene and the ilvD (dihydroxy acid dehydratase, DHAD) gene are externally introduced. According to the recombinant halomonas disclosed by the invention, the L-leucine and / or the L-isoleucine can be efficiently produced without sterilization, in an energy-saving and water-saving manner through open type fermentation, and the industrial production of the recombinant halomonas is facilitated.

The utility model provides a fermentation tank beneficial to improving the density of halophilic strains, belongs to the technical field of halophilic strain fermentation, and aims to solve the problems that the inner wall of the conventional fermentation tank is relatively smooth and the halophilic strains are inconvenient to adhere. Comprising a tank body; a control module is fixedly arranged at the top of the tank cover; a ventilation structure is fixedly arranged between the outer side of the tank body and the interior of the tank cover, a stirring assembly is rotationally arranged in the bottom side of the tank body, and an automatic pressure relief structure is fixedly arranged in the top side of the tank cover; by means of the annular array type attachment protrusions and the attachment grooves, a microstructure can be formed on the inner wall of the tank body, and attachment and growth of halophilic bacteria are facilitated; meanwhile, a ventilation structure and an oxygen sensor are utilized, so that sufficient supply of oxygen in the culture solution can be ensured; a fermentation environment capable of improving the density of halophilic bacteria is provided.

The invention discloses a high-salinity organic wastewater biological treatment method which is characterized by comprising the following steps: step 1, screening halophilic bacteria: directly screening the required halophilic bacteria through soil of saline-alkali soil, and then carrying out secondary screening on flora mixed culture obtained by primary screening; the method has the beneficial effects that halophilic bacteria ZC2403-SYL, ZC2404-SYT and halotolerant bacteria ZC2402-MT which can still degrade pollutants are screened out in a high-salt environment by utilizing a microbial method, organic matters including triethylamine, n-butylamine and formamide can be degraded in the high-salt environment, the degradation rate can reach 90% or above within 72 hours, and the method is simple and convenient to operate and has a wide application prospect. A high-efficiency salt-tolerant biological treatment system and a high-efficiency bacterium, namely a halophilic bacterium system, are constructed aiming at high-salt and refractory organic pollutants, the three strains obtained by screening are mixed and cultured to quickly form a high-salt organic wastewater high-efficiency degradation bacterium agent ZC-SY, and the high-salt organic wastewater high-efficiency degradation bacterium agent ZC-SY is put into actual high-salt organic wastewater to be used, so that stable operation can be achieved. The problem of treating the organic high-salinity wastewater in practice can be effectively solved, and the water treatment cost is reduced.

The invention relates to a hydrogel adsorbent for gold extraction, a preparation method of the hydrogel adsorbent and a gold extraction method, and the preparation method of the hydrogel adsorbent comprises the following steps: concentrating and collecting halophilic bacteria cultured to a stable phase to obtain a halophilic bacteria concentrated solution; expanding and breaking halophilic bacteria cells in the halophilic bacteria concentrated solution by using deionized water to obtain a lysis solution; injecting the lysate into an acidic solution to obtain a hydrogel adsorbent; wherein a reducing substance contained in the hydrogel adsorbent is used for reducing gold ions (III) into a gold elementary substance and fixing the gold elementary substance in the hydrogel adsorbent. According to the embodiment, the hydrogel adsorbent is prepared from the halophilic bacteria, the preparation method is simple, the cost is low, and the preparation efficiency is high; the hydrogel adsorbent contains a reducing substance which can reduce gold ions into gold simple substances and fix the gold simple substances in the hydrogel adsorbent, so that high-efficiency and high-selectivity gold element extraction is realized, the gold extraction cost is effectively reduced, and the gold quality is improved.

The invention provides a method for producing Ectoine through salt impact-glutamine synergistic feeding, and relates to the field of fermentation engineering. The invention particularly relates to a metabolism regulation strategy of salt impact in cooperation with glutamine supplement. According to the method, after salt impact is applied in the logarithmic phase of halophilic bacteria, glutamine is accurately supplemented to activate a thallus stress synthesis pathway. Through the method, the yield of Ectoin can be remarkably increased, the fermentation period is shortened, and the metabolic activity of thalli is enhanced. The method is simple and convenient to operate and controllable in cost, and an innovative process solution is provided for efficient production of industrial fermentation of the halophilic bacteria.

The invention discloses a composite denitrification starting bacterial agent as well as a preparation method and application thereof, and belongs to the field of water pollution control. The method is used for rapidly removing nitrogen oxides in a high-salt degradation-resistant water body low-temperature environment. The microbial inoculum is prepared by compounding three functional strains, namely, pseudoarthrobacter coldtolerant LH-LT-1, petroleum terrestrial bacillus LH-HF0022 and LH-SA-1 (alkaline halophilic bacteria LH-SA-1), wherein the three functional strains are respectively a pseudoarthrobacter coldtolerant LH-LT-1 and a petroleum terrestrial bacillus LH-HF0022. The composite starting microbial agent can tolerate extreme conditions, can activate a denitrification path within 2 hours by adding 3-4 kg / m < 3 > of the composite starting microbial agent at the low temperature of 4 DEG C, and strengthens B / Clt; the method is suitable for scenes of pharmaceutical wastewater, mariculture tail water and the like, and has the advantages of high efficiency, rapidness and low cost.

The invention relates to the technical field of bioengineering, in particular to a recombinant halophilic bacterium for producing PHBV by using acetate as a single carbon source and application of the recombinant halophilic bacterium. The expression and / or activity of 2-methyl citrate synthetase and succinate dehydrogenase assembly factor 2 of the recombinant halophilic bacteria provided by the invention are reduced, 3-hydroxyvaleric acid synthetic genes scpA and scpB are overexpressed, an ADP-dependent acetyl coenzyme A synthetase gene acd is overexpressed, and a heterologous monocarboxylic acid transporter gene mctC is expressed. The strain can generate PHBV without adding precursors such as propionic acid and valeric acid, acetic acid or acetate can be used as a sole carbon source to produce PHBV, the yield is close to that when glucose is used as a carbon source, and the problems that the added precursor substances have certain toxicity, so that the growth of thalli is influenced, and the yield is reduced are solved; environmental pollution caused by addition of precursor substances is avoided, and meanwhile, the fermentation production cost of the PHBV is effectively reduced.

This invention discloses a biological coupling treatment device and method for high-salt organic wastewater, relating to the field of wastewater treatment technology. The biological coupling treatment includes a pretreatment unit, a biological treatment unit, a physicochemical treatment unit, a desalination unit, and a post-treatment unit. The biological treatment unit includes a halophilic bacteria bioreactor and a biofilm reactor. The biofilm reactor includes a bioreactor tank and an MBR membrane module. The bioreactor tank contains activated sludge. The bottom of the MBR membrane module is equipped with an aeration component and a limiting component. The bottom of the bioreactor tank is equipped with a flow propulsion component. The MBR membrane module includes a frame. This invention effectively reduces inorganic salt scaling and organic matter deposition on the surface of the MBR membrane, ensuring the permeability of the MBR membrane, reducing the cleaning frequency, and extending the membrane's service life. In addition, by optimizing the aeration structure, the bubble distribution is uniform, ensuring that the oxygen content in each area remains uniform.

The invention relates to a meadow alkaline halophilic bacterium LH-SA-1 capable of absorbing sodium and reducing salt and alkali as well as a saline-alkali soil remediation microbial inoculum and application of the meadow alkaline halophilic bacterium LH-SA-1. The LH-SA-1 is separated from severe saline alkali soil in Jilin Baicheng, the classification name of the LH-SA-1 is Alkalihalophilus lindianensis, and the preservation number of the LH-SA-1 is CGMCC (China General Microbiological Culture Collection Center) No. 32707. The LH-SA-1 can tolerate 3%-45% of various salts such as sodium chloride, sodium sulfate, sodium carbonate and sodium bicarbonate and a strong alkali environment with the pH value of 9-12. The granular saline-alkali soil remediation microbial inoculum is prepared through composite sodium ion induced fermentation and double-membrane coupling concentration technologies in combination with a multifunctional carrier, the bacterial quantity is larger than or equal to 200 * 10 < 8 > CFU / g, and the catalase activity is larger than or equal to 130000 U / g. The microbial agent is applied to soda saline-alkali soil or coastal composite saline-alkali soil in northeast China, has multiple functions of remarkably reducing the saline-alkali content of the soil, improving the porosity of the soil, improving the activity of enzymes such as catalase, peroxidase, urease and alkaline phosphatase in the soil, improving the content of indoleacetic acid in the soil and the like, and provides an efficient and low-cost biological solution for saline-alkali remediation.

The invention relates to the field of microbial fermentation engineering, and particularly discloses a stirring paddle combination method and system for optimizing fermentation of halophilic bacteria to produce Ectoine. Through CFD simulation and comparison of eight three-layer paddle combinations, it is found that a CD6-RT-RT combination with a semicircular tube paddle (CD6) arranged on the bottom layer and six flat blade paddles (RTs) arranged on the middle layer and the top layer can synergistically improve the gas-liquid mass transfer efficiency of a high-salt system, bubbles are broken through strong radial flow of the CD6 paddle on the top layer, and a gas-liquid interface is expanded in combination with high-shear axial flow of the RT paddles on the middle layer and the bottom layer; uniform distribution of the gas content (gradient is less than or equal to 5%) and kLegt are realized; compared with a traditional combination, the efficiency is improved by 25%-72%; in a 50L tank, the yield of Ectocaine reaches 32.2 g / L, which is obviously improved by 15.0% compared with that of a conventional RT-RT-RT combination. According to the method, the oxygen transfer bottleneck of high-salt fermentation is improved, and an efficient and low-consumption scheme is provided for industrial production of Ectoin.

This invention discloses a method and application for treating moldy corn to synthesize polyhydroxy fatty acids (PHA) using a compound enzyme preparation, relating to the fields of fermentation engineering and waste resource utilization technology. The method for treating moldy corn to synthesize PHA using a compound enzyme preparation includes the following steps: 1) screening and culturing highly efficient halophilic bacteria as inoculum strains for open-enzyme PHA synthesis; 2) pretreating moldy corn with a compound enzyme preparation composed of α-amylase, β-amylase, and glucoamylase; 3) using the moldy corn hydrolysate as a fermentation carbon source, inoculating with PHA-producing halophilic bacteria for open-enzyme fermentation. This invention constructs a complete process system of specific halophilic bacteria screening – moldy corn enzymatic pretreatment – ​​open-enzyme fermentation to synthesize PHA, realizing the resource utilization and high-value utilization of moldy corn. Compared with existing technologies, it has significant economic, environmental, and industrial value, with outstanding comprehensive beneficial effects.

The invention provides a method for improving fermentation performance of halophilic bacteria under salt stress and application, relates to the field of fermentation engineering, and particularly relates to application of a citric acid and betaine mixed additive in improving the fermentation performance of the halophilic bacteria under the salt stress. According to the method, a mixture of citric acid and betaine is added in the growth delay period of halophilic bacteria. The improved fermentation performance can include improvement of the specific growth rate of halophilic bacteria, improvement of the product synthesis rate, shortening of the fermentation period, reduction of the fermentation cost and the like. The method is simple, safe and easy to operate, and an effective regulation and control means is provided for the halophilic bacteria to cope with the high-salt environment in the industrial fermentation process.

The utility model provides a cultivation device for halophilic bacteria in a high-salinity environment, and relates to the technical field of high-salinity wastewater treatment. An inner container is arranged in the cultivation box, first motor seats are installed on the inner walls of the left side and the right side of the inner container through bolts, first motors are installed at the lower ends of the first motor seats through bolts, and bearing seats are installed on the inner walls of the left side and the right side of the inner container through bolts; a threaded rod is mounted between the bearing seat and the first motor seat through a bearing; a culture solution is quantitatively added into the culture dish through the filling head, the top cover is in a closed state in the nutrient solution adding process, the opportunity that halophilic bacteria make contact with the external environment is reduced, the pollution risk of the halophilic bacteria is reduced, the first transmission belt is operated by operating the second motor, the front-back movement of the filling head can be achieved, and the filling efficiency is improved. A third motor is operated to enable a second transmission belt to operate, the filling head can move left and right, and quantitative adding of the nutrient solution to the culture dish is facilitated.

The present invention relates to a hydrogel adsorbent for gold extraction, a preparation method thereof, and a gold extraction method. The preparation method of the hydrogel adsorbent includes: concentrating and collecting halophilic bacteria cultured to the stationary phase to obtain a concentrated halophilic bacteria solution; using deionized water to swell and rupture the halophilic bacteria cells in the concentrated halophilic bacteria solution to obtain a lysate; injecting the lysate into an acidic solution to obtain a hydrogel adsorbent; wherein, the reducing substances contained in the hydrogel adsorbent reduce gold ions (III) to elemental gold and fix the elemental gold inside the hydrogel adsorbent. In this embodiment, a hydrogel adsorbent is prepared using halophilic bacteria. The preparation method is simple, with low cost and high preparation efficiency; the hydrogel adsorbent contains reducing substances, which can reduce gold ions to elemental gold and fix the elemental gold inside the hydrogel adsorbent, thereby realizing efficient and highly selective extraction of gold elements, effectively reducing the extraction cost of gold, and improving the quality of gold.

The invention relates to an Ectoine-producing halophilic bacterium as well as a screening method and application thereof, and belongs to the technical field of microorganisms. According to the Ectoin-producing halophilic bacteria disclosed by the invention, the Ectoin-producing halophilic bacteria are Vreelandelassp., and the Ectoin-producing halophilic bacteria are Ectoin-producing halophilic bacteria. The 16S rRNA of the Ectoin-producing halophilic bacteria has at least 90% of nucleotide sequence consistency with that of SEQ ID NO. 1; the Ectoin halophilic bacteria are used for expressing 2-aminobutyric acid acetyltransferase, 2-aminobutyric acid transaminase and Ectoin synthetase. Through an efficient screening method and deep genome analysis, the novel halophilic strain capable of synthesizing the Ectoine is successfully screened out, and the gene cluster for synthesizing the Ectoine is excavated, so that the production efficiency and yield of the Ectoine can be improved, a microbial resource library is enriched, and the genetic diversity is increased.

The invention discloses a method for producing carotenoid by enriching halophilic flora by using a UASB (Upflow Anaerobic Sludge Blanket) reactor. The method comprises the following steps: (1) adding NaCl, MgSO4. 7H2O, KCl and FeSO4 into deionized water to prepare an inorganic culture medium; (2) adding a yeast extract, casein amino acid and sodium citrate into the inorganic culture medium in the step (1) to prepare an enrichment culture medium, and performing initial inoculation source enrichment in a shake flask; and (3) preparing a simulated wastewater culture medium, adding the simulated wastewater culture medium into the UASB reactor, inoculating halophilic flora, and enriching to produce carotenoid. The method has the characteristics of low energy consumption, simplicity in regulation and control and low possibility of being polluted by infectious microbes.

The invention discloses a water quality purifying agent based on microalgae fermentation. The water quality purifying agent is prepared from alkaline protease, cellulase, a spirulina extract, bacillus subtilis, geobacillus, bacillus amyloliquefaciens, thermophilic bacteria, halotolerant bacteria, nano calcium carbonate, chitosan and spirulina micro powder. Microalgae, microorganisms and an enzyme preparation are adopted to form a synergistic system, the purification effect stability is improved, the efficient and long-acting water quality purification requirements in an extreme environment are met, and the water quality purifying agent has dynamic adaptability, synergistic interaction and environmental friendliness. The preparation method comprises the following steps: directional pretreatment of spirulina, construction of a dynamic flora proportion regulation and control system, culture of complex functional flora, preparation of a nano composite carrier, preparation of a bacterium-algae enzyme complexing agent and the like.

The invention discloses a recombinant halomonas for producing mevalonic acid. The recombinant halomonas is used for expressing an HMG-CoA synthetase gene, namely an EfmvaS gene, from enterococcus faecalis and an HMG-CoA reductase gene, namely an HMGR gene, from rhodobacter marinus. The invention also discloses a preparation method of the recombinant halomonas for producing the mevalonic acid. The recombinant halomonas is used for producing the mevalonic acid. The recombinant halomonas is used for producing the mevalonic acid, and the recombinant halomonas is used for producing the mevalonic acid, wherein the recombinant halomonas is used for expressing the HMG-CoA synthetase gene, namely the EfmvaS gene, from enterococcus faecalis; the invention also relates to a method for producing mevalonic acid. The method comprises the step of fermenting and culturing the recombinant halomonas disclosed by the invention.

The invention belongs to the field of new daily chemical materials, and discloses a sensitive skin anti-aging composition, application and cosmetics, the composition comprises a halophilic bacteria fermentation product and acetyl hexapeptide-8; the weight ratio of the halophilic bacteria fermentation product to the acetyl hexapeptide-8 is 1: (0.1-10). The composition has no irritation, is suitable for sensitive skin, has good improvement effects on crow's feet, subocular wrinkles and compactness, and is very high in improvement speed.

This invention relates to the field of wastewater treatment technology, specifically disclosing a treatment process for high-sulfate organic wastewater. The invention first uses electrodialysis to separate the high-sulfate organic wastewater into two streams under an electric field: sulfate ions are transferred from the high-sulfate organic wastewater to the purified water, resulting in a sulfate-rich concentrate; the high-sulfate organic wastewater then becomes a COD-rich desalination solution, which can be discharged in compliance with standards through conventional biological treatment. The sulfate-rich high-salt concentrate also includes some transferred COD and ammonia nitrogen, which are then treated biologically using halophilic bacteria tolerant to high salt concentrations to oxidize the COD and ammonia nitrogen. The ammonia nitrogen is oxidized to nitrate nitrogen, which is then connected to a sulfur autotrophic denitrification process to remove total nitrogen. The sulfur recovered from the biological treatment of the desalination solution serves as a packing material and electron donor for sulfur autotrophic bacteria, converting NO3- into nitrogen. ‑ -N is reduced to N2 without the need for an external carbon source, and at the same time, total nitrogen emissions meet the standards and sulfur resources are recycled, which has broad application prospects.

This invention belongs to the field of industrial wastewater treatment technology, specifically relating to a method and system for treating wastewater with high salinity (>20 g / L) and high hardness (Ca²⁺>500 mg / L). The invention involves passing wastewater into a bioreactor filled with functionalized microbial capsules. The capsules are prepared by: inoculating halophilic bacteria into a culture medium containing NaCl and trehalose; mixing the resulting bacterial solution with sodium alginate and then adding it dropwise into a CaCl₂ solution; immersing the capsules in a mixture containing polyaspartic acid and magnesium aluminum hydrotalcite to form anti-scaling microspheres; adding carboxylated carbon nanotubes to aniline phosphate buffer, then adding ammonium persulfate, and depositing a polyaniline-carbon nanotube composite conductive layer on the outer periphery of the anti-scaling microspheres; finally, solidifying the microspheres in a copolymer prepolymer solution to form a responsive outer shell. This invention achieves full-process optimization from strain domestication to stable operation by combining functionalized microbial capsules with staged salinity-electric field synergistic regulation, enabling long-term stable treatment of high-salinity and high-hardness wastewater.

The application provides a method for producing 3-hydroxybutyric acid and 3-hydroxyhexanoic acid copolymer PHBHHx, the production of PHBHHx is realized by heterologous expression of PHA synthase PhaC and enoyl-CoA hydratase PhaJ, and the endogenous enoyl-CoA hydratase FadB of halophilic bacteria is inactivated, and / or the composition of the related carbon source is adjusted to control the 3HHx molar ratio in PHBHHx, so as to regulate the material performance of PHBHHx.

The invention provides a carbon organic fertilizer containing medium and low temperature carbonized charcoal coupled animal source organic matter and a preparation method thereof. The carbon organic fertilizer comprises the following components: charcoal and a livestock manure fermentation product, the livestock and poultry manure fermented product is prepared by a method comprising the following steps: mixing livestock and poultry manure with zymophyte, and fermenting to obtain the livestock and poultry manure fermented product, the zymophyte comprises first zymophyte and second zymophyte; the first zymophyte comprises bacillus subtilis, bacillus stearothermophilus and gram positive actinomycetes; the second zymophyte comprises any one or a combination of at least two of bacillus thuringiensis, brown rot fungi or halophilic bacteria. The combination of the charcoal and the fermented livestock manure can increase the content of organic matters in soil for a long time, improve the aggregate structure of the soil, enhance the air permeability and water retention capacity of the soil, reduce the problem of soil hardening, promote the growth of roots and buds, increase the yield of crops and improve the quality of the crops.