21 results about "Halophilic bacteria" patented technology

The invention discloses a method for preparing a carbon monoxide normal-temperature oxidation catalyst by using electrolytic manganese residues and oil sludge, which comprises the following steps: uniformly mixing the oil sludge and the electrolytic manganese residues to obtain manganese oil sludge, uniformly stirring the manganese oil sludge and mixed halophilic desulfurizing bacteria, fermenting, and drying to constant weight to obtain fermented manganese oil sludge, the method comprises the following steps: carbonizing dry fermented manganese oil sludge to obtain a manganese carbide oil material, uniformly mixing the manganese carbide oil material with a blueberry extract to obtain a manganese carbide oil extract mixture, uniformly mixing and stirring the obtained manganese carbide oil extract mixture with a chloroplatinic acid solution, standing, filtering, washing, and drying to obtain the mixed halophilic desulfurizing bacteria. Drying to constant weight to obtain a platinum-doped carbonization extraction precursor, and finally roasting the platinum-doped carbonization extraction precursor to obtain the carbon monoxide oxidation catalyst. The composite catalyst with high specific surface area and multiple active sites is formed, and resource utilization of waste is achieved.

The invention discloses a saline-alkali soil raw water double-micro-flooding root-promoting technology. According to the method, 150-1500 kg of a soil conditioner, 30-150 kg of a soil remediation agent and 450-1500 kg of a soil biological conditioning fertilizer are added into per hectare of original soil according to the actual salinity condition of farmland soil. According to the actual condition of crop cultivation, ditching, ridging or bedding, drip irrigation tape laying, irrigation and seeding are carried out; and the halophilic bacteria agent is conveyed and applied through a drip irrigation belt after being uniformly stirred. By synergistically promoting metabolism of halophilic microorganisms to remove salt and improve rhizosphere microenvironment of crops, root growth is facilitated, saline-alkaline resistance of the crops is improved, and high yield and high quality of the crops are achieved. The technology does not need soil dressing, needs to consume a large amount of fresh water resources, does not have complex treatment engineering, and saves cost investment. The saline-alkali soil is improved gradually and effectively while the yield and economic benefits are obtained freely through saline-alkali soil planting, and a breakthrough positive contribution is made to planting crops on the saline-alkali soil.

This invention discloses an extremely salt-tolerant halophilic bacterium, HD16, which can grow under extreme salinity conditions up to 4.0 M sodium chloride (approximately 230 g / L sodium chloride) and also exhibits high alkalinity tolerance, surviving at pH 7–11. Therefore, it is predicted to have broad application prospects in wastewater treatment and other fields. Furthermore, it can be used to produce polyhydroxyalkali (PHA). When cultured in a 500 ml Erlenmeyer flask, the cell dry weight yield reaches 9.45 g / L, with PHA accounting for 72.97% of the bacterial dry weight. The extreme salt tolerance and high alkalinity tolerance of this strain enable it to efficiently accumulate polyhydroxyalkali esters under open, non-sterile conditions.

The invention discloses a composite denitrification starting bacterial agent as well as a preparation method and application thereof, and belongs to the field of water pollution control. The method is used for rapidly removing nitrogen oxides in a high-salt degradation-resistant water body low-temperature environment. The microbial inoculum is prepared by compounding three functional strains, namely, pseudoarthrobacter coldtolerant LH-LT-1, petroleum terrestrial bacillus LH-HF0022 and LH-SA-1 (alkaline halophilic bacteria LH-SA-1), wherein the three functional strains are respectively a pseudoarthrobacter coldtolerant LH-LT-1 and a petroleum terrestrial bacillus LH-HF0022. The composite starting microbial agent can tolerate extreme conditions, can activate a denitrification path within 2 hours by adding 3-4 kg / m < 3 > of the composite starting microbial agent at the low temperature of 4 DEG C, and strengthens B / Clt; the method is suitable for scenes of pharmaceutical wastewater, mariculture tail water and the like, and has the advantages of high efficiency, rapidness and low cost.

The invention relates to the technical field of bioengineering, in particular to a recombinant halophilic bacterium for producing PHBV by using acetate as a single carbon source and application of the recombinant halophilic bacterium. The expression and / or activity of 2-methyl citrate synthetase and succinate dehydrogenase assembly factor 2 of the recombinant halophilic bacteria provided by the invention are reduced, 3-hydroxyvaleric acid synthetic genes scpA and scpB are overexpressed, an ADP-dependent acetyl coenzyme A synthetase gene acd is overexpressed, and a heterologous monocarboxylic acid transporter gene mctC is expressed. The strain can generate PHBV without adding precursors such as propionic acid and valeric acid, acetic acid or acetate can be used as a sole carbon source to produce PHBV, the yield is close to that when glucose is used as a carbon source, and the problems that the added precursor substances have certain toxicity, so that the growth of thalli is influenced, and the yield is reduced are solved; environmental pollution caused by addition of precursor substances is avoided, and meanwhile, the fermentation production cost of the PHBV is effectively reduced.

The invention relates to a meadow alkaline halophilic bacterium LH-SA-1 capable of absorbing sodium and reducing salt and alkali as well as a saline-alkali soil remediation microbial inoculum and application of the meadow alkaline halophilic bacterium LH-SA-1. The LH-SA-1 is separated from severe saline alkali soil in Jilin Baicheng, the classification name of the LH-SA-1 is Alkalihalophilus lindianensis, and the preservation number of the LH-SA-1 is CGMCC (China General Microbiological Culture Collection Center) No. 32707. The LH-SA-1 can tolerate 3%-45% of various salts such as sodium chloride, sodium sulfate, sodium carbonate and sodium bicarbonate and a strong alkali environment with the pH value of 9-12. The granular saline-alkali soil remediation microbial inoculum is prepared through composite sodium ion induced fermentation and double-membrane coupling concentration technologies in combination with a multifunctional carrier, the bacterial quantity is larger than or equal to 200 * 10 < 8 > CFU / g, and the catalase activity is larger than or equal to 130000 U / g. The microbial agent is applied to soda saline-alkali soil or coastal composite saline-alkali soil in northeast China, has multiple functions of remarkably reducing the saline-alkali content of the soil, improving the porosity of the soil, improving the activity of enzymes such as catalase, peroxidase, urease and alkaline phosphatase in the soil, improving the content of indoleacetic acid in the soil and the like, and provides an efficient and low-cost biological solution for saline-alkali remediation.

This invention discloses a method and application for treating moldy corn to synthesize polyhydroxy fatty acids (PHA) using a compound enzyme preparation, relating to the fields of fermentation engineering and waste resource utilization technology. The method for treating moldy corn to synthesize PHA using a compound enzyme preparation includes the following steps: 1) screening and culturing highly efficient halophilic bacteria as inoculum strains for open-enzyme PHA synthesis; 2) pretreating moldy corn with a compound enzyme preparation composed of α-amylase, β-amylase, and glucoamylase; 3) using the moldy corn hydrolysate as a fermentation carbon source, inoculating with PHA-producing halophilic bacteria for open-enzyme fermentation. This invention constructs a complete process system of specific halophilic bacteria screening – moldy corn enzymatic pretreatment – ​​open-enzyme fermentation to synthesize PHA, realizing the resource utilization and high-value utilization of moldy corn. Compared with existing technologies, it has significant economic, environmental, and industrial value, with outstanding comprehensive beneficial effects.

The invention relates to an Ectoine-producing halophilic bacterium as well as a screening method and application thereof, and belongs to the technical field of microorganisms. According to the Ectoin-producing halophilic bacteria disclosed by the invention, the Ectoin-producing halophilic bacteria are Vreelandelassp., and the Ectoin-producing halophilic bacteria are Ectoin-producing halophilic bacteria. The 16S rRNA of the Ectoin-producing halophilic bacteria has at least 90% of nucleotide sequence consistency with that of SEQ ID NO. 1; the Ectoin halophilic bacteria are used for expressing 2-aminobutyric acid acetyltransferase, 2-aminobutyric acid transaminase and Ectoin synthetase. Through an efficient screening method and deep genome analysis, the novel halophilic strain capable of synthesizing the Ectoine is successfully screened out, and the gene cluster for synthesizing the Ectoine is excavated, so that the production efficiency and yield of the Ectoine can be improved, a microbial resource library is enriched, and the genetic diversity is increased.

The invention discloses a method for producing carotenoid by enriching halophilic flora by using a UASB (Upflow Anaerobic Sludge Blanket) reactor. The method comprises the following steps: (1) adding NaCl, MgSO4. 7H2O, KCl and FeSO4 into deionized water to prepare an inorganic culture medium; (2) adding a yeast extract, casein amino acid and sodium citrate into the inorganic culture medium in the step (1) to prepare an enrichment culture medium, and performing initial inoculation source enrichment in a shake flask; and (3) preparing a simulated wastewater culture medium, adding the simulated wastewater culture medium into the UASB reactor, inoculating halophilic flora, and enriching to produce carotenoid. The method has the characteristics of low energy consumption, simplicity in regulation and control and low possibility of being polluted by infectious microbes.

The invention discloses a water quality purifying agent based on microalgae fermentation. The water quality purifying agent is prepared from alkaline protease, cellulase, a spirulina extract, bacillus subtilis, geobacillus, bacillus amyloliquefaciens, thermophilic bacteria, halotolerant bacteria, nano calcium carbonate, chitosan and spirulina micro powder. Microalgae, microorganisms and an enzyme preparation are adopted to form a synergistic system, the purification effect stability is improved, the efficient and long-acting water quality purification requirements in an extreme environment are met, and the water quality purifying agent has dynamic adaptability, synergistic interaction and environmental friendliness. The preparation method comprises the following steps: directional pretreatment of spirulina, construction of a dynamic flora proportion regulation and control system, culture of complex functional flora, preparation of a nano composite carrier, preparation of a bacterium-algae enzyme complexing agent and the like.

This invention relates to the field of wastewater treatment technology, specifically disclosing a treatment process for high-sulfate organic wastewater. The invention first uses electrodialysis to separate the high-sulfate organic wastewater into two streams under an electric field: sulfate ions are transferred from the high-sulfate organic wastewater to the purified water, resulting in a sulfate-rich concentrate; the high-sulfate organic wastewater then becomes a COD-rich desalination solution, which can be discharged in compliance with standards through conventional biological treatment. The sulfate-rich high-salt concentrate also includes some transferred COD and ammonia nitrogen, which are then treated biologically using halophilic bacteria tolerant to high salt concentrations to oxidize the COD and ammonia nitrogen. The ammonia nitrogen is oxidized to nitrate nitrogen, which is then connected to a sulfur autotrophic denitrification process to remove total nitrogen. The sulfur recovered from the biological treatment of the desalination solution serves as a packing material and electron donor for sulfur autotrophic bacteria, converting NO3- into nitrogen. ‑ -N is reduced to N2 without the need for an external carbon source, and at the same time, total nitrogen emissions meet the standards and sulfur resources are recycled, which has broad application prospects.

The application provides a method for producing 3-hydroxybutyric acid and 3-hydroxyhexanoic acid copolymer PHBHHx, the production of PHBHHx is realized by heterologous expression of PHA synthase PhaC and enoyl-CoA hydratase PhaJ, and the endogenous enoyl-CoA hydratase FadB of halophilic bacteria is inactivated, and / or the composition of the related carbon source is adjusted to control the 3HHx molar ratio in PHBHHx, so as to regulate the material performance of PHBHHx.

The invention provides a carbon organic fertilizer containing medium and low temperature carbonized charcoal coupled animal source organic matter and a preparation method thereof. The carbon organic fertilizer comprises the following components: charcoal and a livestock manure fermentation product, the livestock and poultry manure fermented product is prepared by a method comprising the following steps: mixing livestock and poultry manure with zymophyte, and fermenting to obtain the livestock and poultry manure fermented product, the zymophyte comprises first zymophyte and second zymophyte; the first zymophyte comprises bacillus subtilis, bacillus stearothermophilus and gram positive actinomycetes; the second zymophyte comprises any one or a combination of at least two of bacillus thuringiensis, brown rot fungi or halophilic bacteria. The combination of the charcoal and the fermented livestock manure can increase the content of organic matters in soil for a long time, improve the aggregate structure of the soil, enhance the air permeability and water retention capacity of the soil, reduce the problem of soil hardening, promote the growth of roots and buds, increase the yield of crops and improve the quality of the crops.

The present application relates to the technical field of biological fermentation, and particularly relates to a cell wall breaking wastewater treatment method and application thereof in halophilic bacteria fermentation, wherein the cell wall breaking wastewater is obtained by cell wall breaking of halophilic bacteria fermentation liquor, and the treatment method comprises the following steps: performing rough filtration on the cell wall breaking wastewater to remove solid suspensions and insoluble impurities in the cell wall breaking wastewater, and then performing membrane filtration on the cell wall breaking wastewater by using a nanofiltration membrane with a cut-off average molecular weight of 700 Da or less, so as to obtain a salt-containing clear liquid; further, the salt-containing clear liquid can be partially or entirely used as a solvent of a fermentation culture medium for halophilic bacteria fermentation process. Through the specific wastewater treatment method, the cell wall breaking wastewater in the halophilic bacteria fermentation process can be recycled, and the PHA yield can be effectively ensured, and even the growth rate of the production strain can be improved.

The application belongs to the technical field of soil remediation and agricultural resource utilization, and particularly relates to a saline-alkali soil improvement method based on agricultural waste resource utilization, which comprises the following steps: performing structural dissociation on the waste by physical shearing and high-pressure steam flash explosion, inoculating halophilic bacteria to construct an active carrier, then grafting a pH response monomer and chelating nutrient elements to form a hierarchical coating material with a dynamic feedback mechanism, the material can sense the soil pH value, actively adjust the acidic components and nutrient release, and construct organic-inorganic complexes through degradation products, and the intelligent adjustment is realized through a sensor network. By using the above technical scheme, the application realizes dynamic matching of the improvement release kinetics and the crop rhizosphere demand, effectively solves the problems of instantaneous annihilation of the improver and biological conversion lag, and has the effects of long-term repair and deep remodeling of the soil physical and chemical properties.

This invention provides a method for synthesizing ectoine using moderately halophilic bacteria through fermentation. It innovatively introduces a concentration reaction system, integrating the "cultivation + concentration" of halophilic bacteria into one process. This eliminates the traditional steps of batch feeding and concentration, greatly increasing the yield and efficiency of ectoine synthesis. It enables inexpensive wild-type halophilic bacteria to achieve a level comparable to that of expensive genetically engineered halophilic bacteria, thus significantly reducing the cost of ectoine synthesis.

The invention relates to a recombinant halomonas. The recombinant halomonas comprises an alsS (acetolactate synthase) gene, an ilvC (acetohydroxyacid isoreductase) gene, an ilvD (dihydroxy acid dehydratase) gene and an ilvE (transaminase) gene which are externally introduced. According to the recombinant halomonas disclosed by the invention, the L-valine can be efficiently produced without sterilization, in an energy-saving and water-saving manner through open fermentation, and the industrial production of the L-valine is facilitated.

The invention belongs to the technical field of saline-alkali soil biological fertilizer preparation, and discloses a compound oligosaccharide microelement fertilizer suitable for saline-alkali soil improvement and a preparation and use method thereof. Vitamins, halophilic bacteria and alkaliphilic bacteria are added into the compound fertilizer, salt and alkali in the saline-alkali soil can be effectively absorbed by crops through digested secretions, the saline-alkali soil is effectively improved, and meanwhile the stress resistance of the crops is improved. After the compound fertilizer is added into alkaline land (meanwhile, organic matters in soil are increased), the action mechanism of the compound fertilizer on the saline-alkali soil is to wake up and promote the rapid and healthy proliferation of indigenous beneficial microbial community ecology in the soil, and the microbial communities interact with each other under the promotion of the substances, so that the micro-ecological environment of the soil is improved, and the soil quality is improved. The organic fiber straws are accelerated to be rotted to form organic matters such as organic humic acid, and meanwhile, the stress resistance of crops is improved. The compound fertilizer is simple in use mode and can be used for water-soluble drip irrigation or irrigation after water is sprayed on the ground; the conductivity and the pH value of the saline-alkali soil are obviously reduced after the saline-alkali soil is used for three months.

The invention discloses a production process of nano salt protease, and relates to the technical field of biological fermentation, and the production process comprises the following steps: preparing a culture medium, amplifying strains, and carrying out secondary amplification in a seed fermentation tank to obtain a secondary halophilic bacteria solution; adding a culture medium into a reaction kettle, and then adding the secondary halophilic bacteria solution into the reaction kettle to obtain a mixed solution; fermenting the mixed solution at 28-32 DEG C for 350-400 hours, and adjusting the pH value to 8.2-8.4 by using sodium bicarbonate every 90 hours to obtain a mixed halophilic bacteria solution; carrying out bacterium-enzyme separation on the mixed halophilic bacterium liquid to obtain nano salt protease; the nano salt protease disclosed by the invention can tolerate a high-salt environment, has an ultramicro size of 1-10nm, can permeate into salt crystal micro cracks which cannot be reached by common enzymes, and quickly permeates into a deep layer of soil, so that three-dimensional salt dissolving is realized.

The method provided by the invention can be used for effectively synthesizing a high 3-hydroxyvalerate monomer (3-HV) in a proportion. The method comprises the following steps: adding a 3-HV precursor substance into a fermentation system to prepare PHBV; the precursor substance is one or more of short-chain fatty acids (VFAs). By the adoption of the method, it is found that Halomonas venusta has high tolerance to VFAs, so that the yield of PHBV can be increased; and the proportion of the 3-HV monomer in the product PHBV can be adjusted by changing the mixing proportion of the substrate. Finally, the content of the obtained PHBV is as high as 90.81 wt%, the proportion of 3-HV monomers is as high as 49.81%, and the content of the obtained PHBV is far higher than that of other non-engineering pure bacterium fermentation.

The present invention relates to a recombinant microorganism comprising a metabolic pathway for the synthesis of P34HB (poly-3-hydroxybutyric acid (3HB)-4-hydroxybutyric acid (4HB) ester) wherein the proportion of 4HB is increased by attenuating the synthesis of 3HB, thereby reducing the addition of precursor substances, achieving the purpose that the strain can grow normally and produce a high proportion of P34HB.