Method for synthesizing PHA (polyhydroxyalkanoate) by treating mildewed corn with compound enzyme preparation and application of PHA

By treating with compound enzyme preparations and fermenting with halophilic bacteria, the problems of resource waste and high cost of moldy corn have been solved, and high-value biodegradable plastic PHA has been efficiently converted into agricultural waste, improving the utilization efficiency and safety of agricultural waste.

CN122256444APending Publication Date: 2026-06-23HENAN UNIVERSITY OF TECHNOLOGY
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Patent Information

Application Number
CN202610476210.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-13
Publication Date
2026-06-23

AI Technical Summary

Technical Problem

Existing technologies result in significant waste of resources from moldy corn, and corn-based waste cannot be efficiently converted into high-value products. Furthermore, traditional fermentation methods are costly and lack a complete process system.

Method used

A process system for preparing fermentable sugars from agricultural waste was constructed by treating moldy corn with a compound enzyme preparation, screening for PHA-producing halophilic bacteria strains, enzymatic pretreatment of moldy corn, and open fermentation.

Benefits of technology

It enables the resource-based and high-value utilization of moldy corn, reduces production costs, improves the utilization efficiency of agricultural waste, avoids food safety and environmental pollution risks, achieves efficient starch hydrolysis, and increases PHA production.

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Abstract

The invention discloses a method for synthesizing PHA by treating mildewed corn through a compound enzyme preparation and application, and relates to the technical field of fermentation engineering and waste resource utilization. The method for synthesizing polyhydroxyalkanoate (PHA) by treating mildewed corn with the compound enzyme preparation comprises the following steps: 1) screening and culturing high-efficiency halophilic bacteria as an inoculation strain for open PHA synthesis; (2) carrying out enzymolysis pretreatment on the mildewed corn by adopting a compound enzyme preparation consisting of alpha-amylase, beta-amylase and glucamylase; and (3) inoculating PHA-producing halophilic bacteria to perform open fermentation by taking the mildewed corn hydrolysate as a fermentation carbon source. According to the method, a complete process system of specific halophilic bacteria screening, mildewed corn enzymolysis pretreatment and open type fermentation synthesis of PHA is constructed, recycling and high-value utilization of mildewed corn is achieved, and compared with the prior art, the method has remarkable economic, environmental and industrial values and outstanding comprehensive beneficial effects.
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