The invention relates to a low-toxicity environment-friendly gold mineral separation agent. Urea, sodium carbonate, ferrite, catalysts, sodium cyanate and sodium bicarbonate are adopted as main raw materials, and sodium citrate, thiourea and sodium thiosulfate are selectively added. After reactions of certain steps, the finished product is obtained. In the low-toxicity environment-friendly gold mineral separation agent, sodium cyanide with highly toxic hazards is not used, iron, nickel and carbon catalysts are used for catalyst reactions in the technology process, and the reaction time is shortened; by means of filtering measures, produced unmelted impurities are removed, and the effects of the impurities on gold extraction are avoided; in a product formula, multiple mineral separation ingredients and inhibitors, decomposing agents and the like for mineral harmful impurities are matched, and an obvious leaching effect is achieved for ores difficult to treat; and by means of a special two-time charging manner, the recovery rate of the product is increased.