10 results about "Halophilic microorganisms" patented technology

The invention discloses a saline-alkali soil raw water double-micro-flooding root-promoting technology. According to the method, 150-1500 kg of a soil conditioner, 30-150 kg of a soil remediation agent and 450-1500 kg of a soil biological conditioning fertilizer are added into per hectare of original soil according to the actual salinity condition of farmland soil. According to the actual condition of crop cultivation, ditching, ridging or bedding, drip irrigation tape laying, irrigation and seeding are carried out; and the halophilic bacteria agent is conveyed and applied through a drip irrigation belt after being uniformly stirred. By synergistically promoting metabolism of halophilic microorganisms to remove salt and improve rhizosphere microenvironment of crops, root growth is facilitated, saline-alkaline resistance of the crops is improved, and high yield and high quality of the crops are achieved. The technology does not need soil dressing, needs to consume a large amount of fresh water resources, does not have complex treatment engineering, and saves cost investment. The saline-alkali soil is improved gradually and effectively while the yield and economic benefits are obtained freely through saline-alkali soil planting, and a breakthrough positive contribution is made to planting crops on the saline-alkali soil.

The present invention relates to a method for treatment of poly hydroxy alkanoate (PHA) containing post-consumer product, the method comprising contacting a post-consumer product with a polypeptide that can catalyze degradation of the PHA, the contact taking place at a temperature at least 40° C. and in the presence of salt at a concentration of 1 M or greater. In a specific embodiment, the polypeptide is a wild-type PHA depolymerase expressed by a halophilic microorganism or a modified PHA depolymerase that includes one or more single-site mutations as compared to the wild-type PHA depolymerase. In another specific embodiment, the polypeptide comprising a modified poly hydroxy butyrate (PHB) depolymerase comprising one or more single-site mutations as compared to SEQ ID NO: 1, and the modified PHB depolymerase having a solubility of 10 mg / L or greater. The present invention also relates to a host cell transformed to express a polypeptide that catalyzes degradation of a PHA in the presence of salt at a concentration of 1 M or greater, wherein the host cell is selected from an E. coli cell or a halophilic microorganism.

The invention provides a halophilic organism-based high-salinity wastewater treatment device and a treatment method thereof, and relates to the technical field of coal-fired power plant high-salinity wastewater treatment.The halophilic organism-based high-salinity wastewater treatment device comprises a high-salinity wastewater pool, halophilic plants or salt-tolerant plants are planted in the high-salinity wastewater pool, halophilic microorganisms are added into the high-salinity wastewater pool, and one end of the high-salinity wastewater pool is connected with a water supplementing pipeline; the downstream of the other end of the high-salinity wastewater pool is connected with a drainage pipeline; water quality monitors are arranged on the water supplementing pipeline and the drainage pipeline; the bottom of the high-salinity wastewater pool is covered with a soil layer, a sludge scraper is erected above the soil layer, a sludge pool is arranged at the tail end of the sludge scraper and located on one side of the soil layer, and the sludge pool is communicated with a sludge treatment assembly, so that the problems of secondary pollution of chemical agents, attenuation of membrane flux, frequent maintenance of equipment and high operation and maintenance cost in the prior art are solved; low-cost desalination treatment is achieved, sludge and halophilic plants generated in the biological desalination process can be used as fuel for blending combustion, and carbon emission is reduced.

The present invention relates to an aqueous composition comprising cells of at least one strain of halophilic microorganism and alkaline sulfate at a concentration of at least 30 g / l relative to the total volume of the aqueous composition. The present invention further relates to a method for treating wastewater comprising obtaining or providing wastewater, contacting the wastewater with cells of at least one strain of halophilic microorganism, thereby producing an aqueous composition comprising alkaline sulfate at a concentration of at least 30 g / l, and incubating the aqueous composition under conditions that allow for treatment of the wastewater.

The invention discloses a photoaerobic organism complete mixing reactor for synthesizing purple membrane protein by enriching halophilic microorganisms. The photoaerobic organism complete mixing reactor comprises a biological reaction area and a bottom light management area, the biological reaction area is used for completing microbial cell proliferation, metabolism regulation and control and purple membrane protein synthesis, and providing a limit environment with constant temperature, controllable dissolved oxygen, uniform nutrition and sufficient illumination for halophilic microbial flora so as to realize accurate control of high-density culture and efficient induction of thalli; the bottom light management area is used for efficiently, uniformly and controllably providing light energy with the wavelength and intensity of 200-700nm to the biological reaction area, and the light energy is used as an environmental signal for inducing halophilic microorganisms to synthesize purple membrane protein. According to the reactor, the halophilic microbial flora for efficiently synthesizing the purple membrane protein can be obtained, an aerobic / illumination synergistic condition required by the purple membrane protein synthesis process is provided, sufficient salt and nutrient substances are given to the halophilic microbial flora, and a reactor operation mode for stably and efficiently synthesizing the purple membrane protein at low cost is realized.

The invention relates to the technical field of soil conditioning, in particular to a manufacturing technology and application of a multifunctional soil conditioner. The multifunctional soil conditioner is prepared from the following components in percentage by mass: 40 to 45 percent of modified nano gypsum core, 28 to 30 percent of halophilic microbial agent master batch, 11 to 12 percent of sword bean powder extracting solution, 5 to 8 percent of urea, 4 to 6 percent of calcium chloride, 3 to 5 percent of polyethylene glycol 2000 and 1 to 2 percent of alkyl glycoside, efficient replacement and rapid salt reduction of sodium ions are achieved through a modified nano gypsum core, mineralized shells are generated in situ through an enzyme-induced calcium carbonate precipitation technology, the mineralized shells are cemented with soil particles to form a stable aggregate structure, and long-acting remediation of soil micro-ecology is achieved through halophilic microbial agent master batches. The key problems that a traditional modifier is single in function, chemical improvement and biological improvement are difficult to be compatible, and the improvement effect is not lasting are solved, and an efficient, stable and eco-friendly brand-new strategy is provided for saline-alkali soil treatment.

The invention relates to a method for efficiently synthesizing purple membrane protein by using photosensitive halophilic microorganisms. The preparation method comprises the following steps: (1) adding casein amino acid, a yeast extract, sodium citrate, MgSO4. 7H2O, KCl, FeSO4 and NaCl into ultrapure water to prepare a high-salt culture medium, and adjusting the pH value to 7.0-7.2 by using NaOH; (2) inoculating the high-salt culture medium with a halobacterium salina flora, and carrying out enrichment culture in a shake flask; (3) MgSO4. 7H2O, KCl, FeSO4 and NaCl are added into ultrapure water, sodium acetate and ammonium chloride are supplemented at the same time, a synthetic culture medium is prepared in the ultrapure water, and the pH is adjusted to 7.0-7.2 with NaOH; (4) A, introducing the synthetic culture medium in the step (3) into a shake flask inoculated with halobacterium salina groups, and carrying out enrichment culture in a constant-temperature shaking table: B, dividing the culture conditions in the step A into an illumination group and a dark group according to whether illumination is provided or not, and keeping the operation at a high-speed rotating speed without changing other conditions; and C, carrying out stable operation of shake-flask culture in the step B, and finally synthesizing the purple membrane protein. The method effectively improves the yield of purple membrane protein.

The present invention relates to halophilic microorganisms. In particular, halophilic microorganisms that expresses citramalate synthase. A plasmid encoding an antitoxin-toxin system, and a halophilic microorganism comprising said plasmid are also described, and methods of producing halophilic organisms are also described herein.

This invention provides a MOF-based halophilic microbial inoculant, its preparation method, and its application, relating to the field of microbial technology. The preparation method of the MOF-based halophilic microbial inoculant of this invention includes the following steps: mixing a halophilic bacterial suspension with a metal-organic framework (MOF) for reaction; then coating the surface of the microbial cell with the MOF to form a MOF shell with a thickness of 0.01–6.0 μm, thereby obtaining the MOF-based halophilic microbial inoculant. The MOF-based halophilic microbial inoculant of this invention, through the organic combination between the halophilic bacterial strain and the metal-organic framework, can reduce the impact of external stimuli on the activity of the halophilic strain, maintain the activity of the halophilic strain under high salt stress conditions, and thus alleviate the damage caused by salt stress to plants.

A method for producing a polyhydroxyalkanoate from biomass is described herein. The method can include providing a biomass stream; treating the biomass stream in an anaerobic digestion reactor to provide a treated biomass stream comprising volatile fatty acids; contacting the treated biomass stream with halophilic microorganism to produce the polyhydroxyalkanoate intracellularly; and extracting the polyhydroxyalkanoate from the halophilic microorganism.