Improved method for cultivating microorganisms

A technology of halophilic microorganisms and culture medium, which is applied in the field of improved culture of halophilic microorganisms, can solve the problems of reduced dry weight, slowed microbial growth, and no discovery, so as to reduce the concentration of chloride ions, reduce corrosion, and reduce bacterial contamination Effect

Active Publication Date: 2020-05-08
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 2. The corrosion of metals and the adverse effects of chloride ions in salty environments
[0007] 3. Problems caused by high chloride ion fermentation to downstream wastewater treatment
[0009] Although it has been noted that the use of high concentrations of NaCl in the medium will cause the above problems, so far, no satisfactory alternative medium to reduce the

Method used

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  • Improved method for cultivating microorganisms
  • Improved method for cultivating microorganisms
  • Improved method for cultivating microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1. Using Na 2 SO 4 Mix NaCl as sodium salt for fermentation

[0059] In order to reduce the amount of NaCl used, the inventors tried to use NaCl 2 SO 4 Partially or completely replace NaCl to provide a suitable high-salinity environment. At the same time, the experiment using only NaCl as the sodium salt was used as a control. In this experiment, Halomonas bluephagenesis TD01 was used as a fermentation strain for fermentation culture, and the aforementioned MM medium was used as a basal medium, and sodium salt and glucose were added. The culture conditions are shown in Table 1, and shake flask experiments were used. Three parallel samples were set for each experiment, and the results were averaged. The experimental results are shown in Table 1 (wherein the percentage of sodium salt is the mass fraction).

[0060] Table 1:

[0061]

[0062] The results showed that when the NaCl used to provide a high-salinity environment was partially replaced with Na ...

Embodiment 2

[0063] Example 2. Using Na 2 SO 4 Alternative to NaCl as sodium salt for fermentation

[0064] Carry out experiment according to the material and condition described in embodiment 1, difference is to use only Na 2 SO 4 Instead of using NaCl as the sodium salt. Meanwhile, an experiment using only NaCl as the sodium salt was used as a control. Three parallel samples were set for each experiment, and the results were averaged. The results are shown in Table 2.

[0065] Table 2:

[0066]

[0067] The results showed that when NaCl used to provide a high-salinity environment was replaced with Na 2 SO 4 When replaced, the growth and product synthesis of TD01 bacteria were basically not affected. Thus, the corrosion effect of the fermentation system on the fermentation equipment caused by the presence of chloride ions can be reduced, and the concentration of chloride ions in the fermentation system can be reduced to facilitate subsequent wastewater treatment. Moreover, th...

Embodiment 3

[0068] Example 3. Using Na 3 PO 4 Alternative to NaCl as sodium salt for fermentation

[0069] Carry out experiment according to the material and condition described in embodiment 1, difference is to use Na 3 PO 4 Instead of NaCl as sodium salt for fermentation, Na 3 PO 4 The concentration is 55g / L (equivalent to the amount of Na ions provided by 60g / L NaCl).

[0070] The results proved that sodium phosphate can indeed replace sodium chloride for fermentation. The bacteria grew well and the PHA yield was comparable to that obtained when the fermentation was carried out with NaCl, in other words, with NaCl 3 PO 4 Substituting NaCl as sodium salt for fermentation can also reduce the corrosion effect of the fermentation system on the fermentation equipment caused by the presence of chloride ions without affecting the synthesis of the target product, and reduce the concentration of chloride ions in the fermentation system to facilitate subsequent wastewater treatment.

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Abstract

The invention provides an improved method for cultivating halophilic microorganisms. According to the method, NaCl which is conventionally used for providing a high-salt environment for the halophilicmicroorganisms is completely or partially replaced by other sodium salts, and therefore the chloride ion concentration in the fermentation system can be reduced, so that corrosion of chloride ions inthe fermentation system to a fermentation device is reduced, subsequent wastewater treatment is facilitated, and microbial contamination during open fermentation is reduced; and the invention also relates to a culture medium for the cultivation.

Description

technical field [0001] The present invention relates to an improved method for culturing halophilic microorganisms, especially halophilic bacteria. Background technique [0002] 1. Fermentation by halophilic bacteria [0003] In the field of using microbial fermentation to produce target products such as polyhydroxyalkanoate (PHA), the use of halophilic microbial fermentation has the advantages of open fermentation without sterilization, high cell density, and high yield, so it is widely used in the production field. focus on. In the open fermentation of halophilic microorganisms, high-concentration NaCl (such as 60g / L NaCl) is often added to the commonly used medium, which helps to maintain a high extracellular osmotic pressure and provides a relatively suitable environment for halophilic bacteria. External conditions, in order to avoid the influence of other bacteria, to achieve the purpose of open fermentation. [0004] 2. The corrosion of metals and the adverse effect...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 陈国强刘絮
Owner TSINGHUA UNIV
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