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Method for enhancing salt resistance of turf grass by using halophiles

A technology for halophilic microorganisms and turfgrass is applied in the field of using halophilic microorganisms to enhance the salt resistance of turfgrass. , the effect of increasing surface runoff

Inactive Publication Date: 2017-02-22
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, chemical amendments may cause secondary pollution, and physical methods are costly, palliative but not root cause, and cannot fundamentally solve the problem of soil salinization

Method used

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  • Method for enhancing salt resistance of turf grass by using halophiles
  • Method for enhancing salt resistance of turf grass by using halophiles
  • Method for enhancing salt resistance of turf grass by using halophiles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Development materials and methods

[0037] 1) Experimental materials:

[0038] Perennial ryegrass "lark" seeds with full grains and uniform size were selected as experimental materials; normal soil was taken from the nursery of Wuhan Botanical Garden, impurities were removed, passed through a 2mm sieve, placed in a ventilated place for 48-72 hours, and naturally air-dried; saline-alkaline soil was taken from The seaside of Dongying City, Shandong Province has a salt content of 1.89%.

[0039] 2) Preparation of compound halophilic microbial fertilizer:

[0040] Before sowing, pick the strains of halophilic bacteria (Aspergillus aculeatus, Aspergillus aculeatus ) were inoculated in Martin's agar medium, cultured in an incubator at 30°C for 72 hours, then inoculated into liquid medium, and cultured in shake flasks at 30°C for 16.5 hours;

[0041] Centrifuge the above cultured bacterial solution in a centrifuge at 6800-7800r / min for 10 minutes, discard the supernata...

Embodiment 2

[0054] 1. Development materials and methods

[0055] 1) Experimental materials

[0056] Bermudagrass seeds with full grains and uniform size were selected as experimental materials; normal soil was taken from the nursery of Wuhan Botanical Garden, impurities were removed, passed through a 2 mm sieve, placed in a ventilated place for 48-72 hours, and naturally air-dried; saline-alkali soil was taken from the seaside of Dongying City, Shandong Province , with a salt content of 1.89%.

[0057] 2) Preparation of compound halophilic microbial fertilizer

[0058] Before sowing, pick the strains of halophilic bacteria (Aspergillus aculeatus, Aspergillus aculeatus ) were inoculated in Martin's agar medium, cultured in an incubator at 30°C for 72 hours, then inoculated into liquid medium, and cultured in shake flasks at 30°C for 16.5 hours.

[0059] Centrifuge the above cultured bacterial solution in a centrifuge at 6800-7800r / min for 10 minutes, discard the supernatant, collect t...

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Abstract

The invention discloses a method for enhancing salt resistance of turf grass by using halophiles, relating to the technical field of biological restoration of salinized soil. The method comprises the following steps: (1) culture of a halophile bacterial liquid; (2) preparation of a bacterial suspension; (3) preparation of a composite halophile bacterial fertilizer; and (4) turf planting. The fertility condition of soil is improved; the plants adsorb salts; the turf glass can form a compact withered grass layer, thereby increasing the overland runoff of rainfall and reducing the non-point pollution; the microbe culture medium wood dust ensures the supply of microbial growth nutrients, enhances the operability of implanting microbes into soil, is capable of improving the soil environment, and effectively utilizes the wastes; and the method is low in cost and simple to operate.

Description

technical field [0001] The invention relates to the technical field of saline soil bioremediation, in particular to a method for enhancing the salt resistance of turfgrass by using halophilic microorganisms. Background technique [0002] Soil salinization is one of the main types of land desertification and degradation in the world today. Salinized soil seriously damages land resources, affects the growth of crops and food production, and brings great harm to the production of agriculture, forestry and animal husbandry. effects and serious losses. my country is one of the countries with serious saline soil, and the area of ​​saline soil accounts for 69.3% of the country's usable land. The harm of saline soil mainly has three aspects: 1. It is harmful to the development of agriculture, forestry and animal husbandry. Salinized soil cannot provide suitable conditions for the growth of plants. Excessive salt ions will inhibit the growth of plants or even die, thereby reducing t...

Claims

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Application Information

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IPC IPC(8): C05G3/04A01G1/00C12N1/14C12R1/66C05G3/80
CPCA01G20/00C05F5/002C05G3/80C12N1/14C05F11/08
Inventor 傅金民谢燕韩世娟李晓宁
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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