Bacillus polymyxa DYr4.4 with broad spectrum antibacterial activity and preparation method and application

Abstract

The invention discloses bacillus polymyxa DYr4.4 with broad spectrum antibacterial activity and a preparation method and application. The number is CCTCC M 2018023. The method comprises the steps thatA, a single bacterial colony of a bacterial strain is selected under the aseptic environment, inoculated to a culture solution and placed in a constant-temperature culture oscillator, the temperatureand rotation speed are adjusted, and oscillation culture is conducted; B, a fermentation solution is placed in each bottle, the bottles are placed in a large-amplitude constant-temperature freezing shaking table, and oscillation culture is conducted; C, the culture solution is prepared, wherein solutions are added to a tank in sequence, a tank body is sealed, the culture solution is heated and disinfected, an inoculation port is disinfected by igniting alcohol, 2 L of fermentation solution prepared in the last step is poured into the tank under the condition that the alcohol burns continuously, and the rotation speed of a stirrer is adjusted; D, the fermentation solution is filtered, an auxiliary material namely light calcium carbonate powder is added to the filtered fermentation solution, the mixed solution is placed in a centrifugal spray drying tower and dried, and dry powder is collected. The bacillus polymyxa DYr4.4 is applied to preparation of medicine for treating or preventingrust diseases of pear trees. Dry powder fermentation of the bacterial strain has the remarkable anti-disease effect on the rust diseases of the pear trees, the prevention effect reaches 67.09% or above, and leaf falling of the pear trees in advance is remarkably inhibited.

Description

technical field [0001] The invention belongs to the technical field of plant disease biological control, more specifically relates to a broad-spectrum antibacterial activity of Bacillus polymyxa DYr4.4, and also relates to a preparation method of a broad-spectrum antibacterial activity of Bacillus polymyxa DYr4.4 , and further relates to the use of a broad-spectrum antibacterial activity of Bacillus polymyxa DYr4.4. Background technique [0002] Pear tree rust is caused by Gynmosporangium haraeanum Syd.=Gynmosporangium asiaticum Miyabe ex Yamada, which is common in Chinese pear orchards and can cause leaves and fruits to fall off, seriously affecting yield. At present, chemical agents are mainly used for prevention and control in China, such as mancozeb, diniconazole, triadimefon, etc. In addition to reports, biological control is carried out by hyperparasites of pear gum rust (Tuberculina vinosa Sacc.) In addition to control, there is no commercial biocontrol agent. Long-...

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Problems solved by technology

However, there is no report that Paenibacillus polymyxa can be used to control pear tree diseases such as pear rust.

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