Industrial multistage communicated liquid fermentation method for bacillus cereus preparation for dissolving oscillatoria in pond

A technology of Bacillus cereus and Oscillator pond, applied in the field of microbial preparations, can solve problems such as no industrialized production of inoculants, industrialized fermentation production of inoculants, etc., achieve good dissolution specificity, and ensure high purity. Effect

Inactive Publication Date: 2015-11-11
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because not all strains of Bacillus cereus have algicidal properties, and the algicidal bacteria in the above research are not the Bacillus cereus or similar algicidal strains involved in this patent, and the reports on the fermentation research of algicidal bacteria are still Most of them are of research nature, far from meeting the needs of industrialized fermentation production of bacterial agents, and there are no industrialized production products of related bacterial agents

Method used

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  • Industrial multistage communicated liquid fermentation method for bacillus cereus preparation for dissolving oscillatoria in pond
  • Industrial multistage communicated liquid fermentation method for bacillus cereus preparation for dissolving oscillatoria in pond
  • Industrial multistage communicated liquid fermentation method for bacillus cereus preparation for dissolving oscillatoria in pond

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Effect test

Embodiment 1

[0046] The industrialized multi-stage connected liquid fermentation method of the Bacillus cereus preparation provided in this embodiment comprises the following steps:

[0047] (1) Establishment of industrialized liquid fermentation parameters of bacterial agents

[0048] The 50L primary fermentation tank system platform was used to study and establish the process parameters of the industrialized liquid fermentation of bacterial agents. Taking the spore formation rate of Bacillus cereus CZBC1, the algae-lysis efficiency and effect time of inoculant fermentation products as the core indicators, the relevant fermentation parameters were established and optimized.

[0049] First, using the single factor test method, on the basis of the basic fermentation medium, different carbon sources (glucose, sucrose, glucose+sucrose, soluble starch, molasses, corn steep liquor, cornstarch, bran, bran+molasses, Soluble starch + corn steep liquor), different nitrogen sources (peptone, yeast ...

Embodiment 2

[0067] The industrialized multi-stage connected liquid fermentation method of the Bacillus cereus preparation provided in this embodiment comprises the following steps:

[0068] Production and application were carried out in the Huadu Microbial Industrial Fermentation Experimental Factory of the South China Sea Fisheries Research Institute of the Chinese Academy of Fishery Sciences in Huadu District, Guangzhou City and Guangzhou Xinhai Lisheng Biotechnology Co., Ltd. The fermentation system is a multi-stage fermentation system of 50L-500L-5000L Unicom fermentation system.

[0069] (1) First prepare the seed liquid of CZBC1 bacterial strain in the laboratory, and the preparation method of the seed liquid of CZBC1 bacterial strain is the same as that in Example 1.

[0070] (2) The formula of the fermentation medium is 7.5g of glucose, 7.5g of molasses, 5g of yeast extract, 5g of peptone, 20g of soybean protein, 1g of NaCl, KH in 1L of water 2 PO 4 0.2g and MgSO 4 0.2g, accord...

Embodiment 3

[0077] The difference from Example 2 is that the fermentation medium formula contains 0.6g of glucose, 8.5g of molasses, 5g of yeast extract, 7g of peptone, 18g of soybean protein, 1.3g of NaCl, and KH in 1L of water. 2 PO 4 0.15g and MgSO 4 0.3g.

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Abstract

The invention discloses an industrial multistage communicated liquid fermentation method for a bacillus cereus preparation for dissolving oscillatoria in a pond. The industrial multistage communicated liquid fermentation method is characterized in that by taking an inocula industrial liquid fermentation system as a platform, and taking the guarantee of growth performance and algae-lytic characteristic of algicidal bacteria preparation products as an objective, a production process flow of products, including seed liquid culture, multistage communicated fermentation tank culture, bacterial sludge preparation as well as carrier compounding, drying, crushing and packaging, is established, a system solution for industrial liquid fermentative production of the bacillus cereus preparation for dissolving the oscillatoria is provided, and relevant inocula products are formed. According to the method, the demand of the inocula products on industrial liquid fermentative production can be met; on the other hand, the condition that the algae-lytic characteristic and the growth performance of the bacillus cereus preparation products formed by the method disclosed by the invention are influenced by industrial and scale production is avoided; the bacillus cereus preparation products still have good specificity of dissolving the oscillatoria, have no dissolving effect on high-quality green alga and diatom and no adverse effect on culture of prawns, and can be applied to culture production.

Description

technical field [0001] The invention belongs to the technical field of microbial preparations, and in particular relates to an industrialized multi-stage communication liquid fermentation method for dissolving a Bacillus cereus preparation of pond oscillating algae. Background technique [0002] With the rapid development of the aquaculture industry, a large amount of residual bait and metabolites produced during the aquaculture process have caused the eutrophication of pond water to increase, resulting in a large number of cyanobacteria and a high frequency of cyanobacteria blooms (Peng Congcong et al., 2011). Cyanobacteria consume a large amount of oxygen and can release toxic and harmful substances such as cyanotoxins, hydroxylamine, and hydrogen sulfide, which seriously damage the aquaculture water body, cause the poisoning and death of the aquaculture objects, and have a huge impact on the ecological environment of the water body and aquaculture benefits (Liu Xiaozhu et ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/085
Inventor 文国樑曹煜成李卓佳胡晓娟徐煜徐创文
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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