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Industrial production method of selenium-rich lactobacillus rhamnosus and culture medium used by industrial production method

A technology of Lactobacillus rhamnosus and its production method, which is applied in the field of industrialized production method of Lactobacillus rhamnosus enriched in selenium and the culture medium used therein, and can solve problems such as unreported

Pending Publication Date: 2021-03-16
北京硒哥硒生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the industrial production method of selenium-enriched Lactobacillus rhamnosus has not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0084] The preparation method of the selenium-enriched yeast of broken wall in following embodiment is as follows:

[0085] 1. Mix 150kg of selenium-enriched yeast powder (purchased from Angel Yeast Co., Ltd., a product in line with national standards, with an organic selenium content of 2000ppm) and 3000kg of deionized water to make a yeast water clear solution, and stir at room temperature for 2 Hours, the purpose is to make the yeast cells swell and rupture by deionized water (under the deionized water environment, the ions in the cells leave the cell, causing the cells to swell and rupture).

[0086] 2. In order to break the unbroken yeast cells and further increase the effect of breaking the wall, put the yeast liquid liquid in a freezer at -15°C for 0.5 hours after stirring for 2 hours, and then quickly heat it to 80°C in the reaction tank. ℃, keep it for 0.5 hours, and then lower the temperature to 60 ℃ to obtain broken selenium-enriched yeast.

Embodiment 1

[0087] Embodiment 1, the production method of selenium-enriched Lactobacillus rhamnosus and the detection of organic selenium and viable count

[0088] (1) Production method of selenium-enriched Lactobacillus rhamnosus

[0089] 1. Activation of bacteria

[0090] Take out 0.5 g of lyophilized powder of Lactobacillus rhamnosus from the refrigerator, inoculate it into a test tube containing 10 ml of improved MRS seed medium, and let it stand overnight at 30° C. to obtain a bacterial liquid after overnight. The overnight bacterial solution was subcultured once in a test tube (the subculture medium was a modified MRS seed medium, and the culture condition was 30° C. for 15 hours), and the subcultured bacterial solution was obtained. The bacterium liquid after the subculture is inoculated in the Erlenmeyer flask (1000 milliliters) that contains the improved MRS seed culture medium of 400 milliliters, after cultivating 20 hours under the condition of 30 ℃, obtain the Lactobacillus r...

Embodiment 2

[0111] Embodiment 2, the production method of selenium-enriched Lactobacillus rhamnosus and the detection of organic selenium and viable count

[0112] (1) Production method of selenium-enriched Lactobacillus rhamnosus

[0113] The selenium-enriched fermentation medium A in the step 4 of embodiment 1 (one) is replaced by the selenium-enriched fermentation medium B; the solvent of the selenium-enriched fermentation medium B is water, and the solute and mass fraction thereof are respectively as follows: Selenium yeast 4%, beef powder 0.5%, glucose 0.5%, KH 2 PO 4 3%, Tween 80 0.5%, sodium acetate 0.5%, sodium citrate 0.2%, zinc sulfate 0.05%, magnesium sulfate 0.05%, vitamin B1 100ppm, adjust the initial pH value to 6.8.

[0114] Other steps are identical with embodiment 1 (one).

[0115] (2) Detection of organic selenium content in selenium-enriched Lactobacillus rhamnosus products

[0116] According to the method in step (2) in Example 1, the percentage content of organic ...

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Abstract

The invention discloses an industrial production method of selenium-rich lactobacillus rhamnosus and a culture medium used by the industrial production method. The industrial production method comprises the following steps of 1) activating lactobacillus rhamnosus in a seed culture medium to obtain an activated seed solution; 2) carrying out fermentation culture on the activated seed solution in abasic fermentation culture medium to obtain a fermentation solution; and 3) carrying out fermentation culture on the fermentation solution in a selenium-enriched fermentation culture medium to obtaina fermentation product, wherein the selenium-enriched fermentation culture medium contains wall-broken selenium-enriched yeast. High-selenium-content wall-broken yeast is used as a selenium source inthe fermentation culture medium, so that the selenium conversion rate in the lactobacillus rhamnosus fermentation process is effectively increased, and the defect that inorganic sodium selenite remains on the surfaces of thalli after fermentation culture is performed by using sodium selenite as the selenium source in the prior art is overcome. The ratio of organic selenium in the finally obtainedselenium-rich lactobacillus rhamnosus product to total selenium can reach 99.5% or above.

Description

technical field [0001] The invention relates to the field of microbial fermentation engineering, in particular to an industrial production method of selenium-enriched Lactobacillus rhamnosus and a culture medium used therein. Background technique [0002] Lactobacillus rhamnose is a probiotic that can use fermentable sugars to produce lactic acid. It can adhere to intestinal epithelial cells and colonize in the human body, and has a variety of physiological functions. For example: Inhibit the growth of harmful bacteria in the intestinal tract (such as inhibiting the growth of Helicobacter pylori), promote lactose digestion, assist in the treatment of constipation and diarrhea, relieve ulcerative colitis, lower serum cholesterol, anti-cancer, anti-allergic, improve immunity, regulate uric acid metabolism Improve physiological functions such as gout symptoms. [0003] Selenium is an essential trace element for the human body. The human body cannot synthesize it by itself and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/38A23L33/135A61K35/747A61P1/00A61P37/04C12R1/225
CPCC12N1/20C12N1/38A23L33/135A61K35/747A61P1/00A61P37/04A23V2002/00A23V2400/175A23V2200/3204A23V2200/324
Inventor 肖志龙米长江杨建国
Owner 北京硒哥硒生物科技有限公司
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