The invention discloses an acer palmatum Oridono Nishiki twig cutting propagation method. The acer palmatum Oridono Nishiki twig cutting propagation method comprises the following steps that S1, time for cuttage is determined, wherein cutting is conducted in the months from April to August; S2, a cutting medium is prepared and sterilization is conducted, wherein vermiculite serve as the cutting medium, and the cutting medium is sprayed with carbendazim; S3, a scion is collected, wherein a semi-lignified branch, with the thickness ranging from 0.3 cm to 0.6 cm, growing in the very year is selected to serve as the scion; S4, a cutting is prepared; S5, the cutting is sterilized, wherein the two ends of the cutting are soaked in carbendazim; S6, hormone treatment is conducted, wherein the root of the cutting is soaked in the GGR6 solution; S7, cuttage is conducted, wherein the cutting is inserted in the medium; S8, management is conducted after the cutting is inserted into the medium; S9, transplantation is conducted. According to the acer palmatum Oridono Nishiki twig cutting propagation method, the rate of rooting of cuttings reaches over 80%, and the survival rate of transplantation can reach 85%. The acer palmatum Oridono Nishiki twig cutting propagation method has the advantages that the excellent characteristic of the female parent is kept, the reproduction speed is high, the survival rate is high, operation is easy, practicability is high, and the method has high application value in the aspect of vegetative propagation of excellent acer palmatum Oridono Nishiki seedlings.