The invention provides a vernonia amygdalina tea bag and a preparation method thereof. The vernonia amygdalina tea bag comprises vernonia amygdalina, ural licorice roots and prepared adhesive rehmannia roots which are medicinal, edible, free from toxic and side effects and suitable for long-term medication. The vernonia amygdalina serves as a monarch, the prepared adhesive rehmannia roots serve as an adjuvant, the ural licorice roots serve as a conductant, the three plants are matched according to a certain proportion, the cold nature of the vernonia amygdalina can be relieved, the kidney is protected, the stomach is nourished, the symptom of impairment of the kidney caused by coldness due to long-term medication is avoided, and traditional Chinese medicine combination is achieved to realize kidney conditioning and protecting, evil eliminating and no injury. Besides, the prepared adhesive rehmannia roots and the ural licorice roots can obviously reduce bitterness, and made tea water is sweet and a little bitter in taste, sweet after taste and suitable for long-term medication of all crowds except for surgical recovering patients and women in pregnancy and a physiological period. In addition, the vernonia amygdalina tea bag has the function of clearing away heat and toxic materials, can adjust 'hyperlipidemia, hypertension and hyperglycemia', can resist senility and can effectively enhance human immunity functions, the used prepared adhesive rehmannia roots and the ural licorice roots are free from mutual side effects, and healthcare effects are obvious.