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Fermentation medium for promoting inonotus hispidus mycelium growth and generating exopolysaccharides

A fermentation medium and mycelia growth technology, applied in the direction of fermentation, microorganism-based methods, biochemical equipment and methods, etc., to achieve the effects of low production and processing costs, convenient operation, and simple production

Active Publication Date: 2018-08-17
福建原培真牛樟芝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a blank in the research on how to improve the production of mycelium and exospore polysaccharides of P. gracilis

Method used

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  • Fermentation medium for promoting inonotus hispidus mycelium growth and generating exopolysaccharides
  • Fermentation medium for promoting inonotus hispidus mycelium growth and generating exopolysaccharides

Examples

Experimental program
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Effect test

Embodiment 1

[0014] Fermentation medium: Take 200 g of potatoes, cut into small pieces, add 20 g of glucose into 1000 ml of water, boil for 10 minutes, filter the residue, and collect the filtrate; add 0.45 g of Vernonia amygdala, add water to make up to 1000 ml. Sterilize at 121°C for 30 minutes.

[0015] A 250 ml Erlenmeyer flask with a total liquid volume of 100 ml per bottle was used to inoculate P. gracilis into the fermentation medium for fermentation. The inoculum size was 7%, and the fermentation temperature was 25°C.

[0016] The fermentation is shaker culture fermentation, and the rotation speed is 160 rpm / min.

[0017] The measured exopolysaccharide content was 1.62 mg / mL, and the mycelium yield was 1.38 g.

Embodiment 2

[0019] Fermentation medium: Take 200 g of potatoes, cut into small pieces, add 20 g of glucose into 1000 ml of water, boil for 10 minutes, filter the residue, and collect the filtrate; add 0.5 g of Vernonia amygdala, add water to make up to 1000 ml. Sterilize at 121°C for 30 minutes.

[0020] A 250 ml Erlenmeyer flask with a total liquid volume of 100 ml per bottle was used to inoculate P. macropora into the fermentation medium for fermentation. The inoculum size was 7.5%, and the fermentation temperature was 25°C.

[0021] The fermentation is shaker culture fermentation, and the rotation speed is 160 rpm / min.

[0022] The measured exopolysaccharide content was 1.66mg / mL, and the mycelium yield was 1.43g.

Embodiment 3

[0024] Fermentation medium: Take 200 g of potatoes, cut into small pieces, add 20 g of glucose into 1000 ml of water, boil for 10 minutes, filter the residue, and collect the filtrate; add 0.55 g of Vernonia amygdala, add water to make up to 1000 ml. Sterilize at 121°C for 30 minutes.

[0025] A 250 ml Erlenmeyer flask with a total liquid volume of 100 ml per bottle was used to inoculate P. macropora into the fermentation medium for fermentation. The inoculum size was 8%, and the fermentation temperature was 25°C.

[0026] The fermentation is shaker culture fermentation, and the rotation speed is 160 rpm / min.

[0027] The measured exopolysaccharide content was 1.58 mg / mL, and the mycelium yield was 1.41 g.

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Abstract

The invention provides a fermentation medium for promoting inonotus hispidus mycelium growth and generating exopolysaccharides, and belongs to the field of microbial fermentation. A preparation methodof the medium includes the steps: adding 200g of potatoes and 20g of glucose into 1000ml of water together, boiling the water for 10 minutes, filtering waste residues and collecting filtrate; adding0.45-0.55g of vernonia amygdalina, adding water to supplement the filtrate to 1000ml, and performing sterilization for 30 minutes at the temperature of 121 DEG C. The medium is used for liquid fermentation of inonotus hispidus, and under the same culture condition, compared with a reference medium without vernonia amygdalina, the fermentation medium has the advantage that mycelium yield and exopolysaccharide yield are improved by 1.21 times and 2.52 times. The fermentation medium plays an important role in further deep development and application of inonotus hispidus to health food and medicalappliances.

Description

technical field [0001] The invention relates to a fermentation medium for promoting the production of extraspores and mycelia by Porpus pilosula, belonging to the field of microbial fermentation. Background technique [0002] P. gracilis Inonotus hispidus Belongs to Basidiomycota, Agaricaceae, Prussiana, Prussiaceae, Pyroporus. P. gracilis is a facultative saprophytic fungus, mainly parasitic, and a medicinal fungus. Studies have found that it has a wide range of biological activities, such as anti-virus, anti-aging, lowering blood fat, and improving immunity. It has good practical value in functional food and medicine, among which polysaccharides are more abundant Studies have shown that the fermented polysaccharides of P. gracilis has various pharmacological activities such as anti-tumor, anti-oxidation and improving immunity. At the same time, its fermented hyphae and fruiting bodies have similar components and activities, and are very good for fruiting bodies. Good su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12N1/14C12R1/645
CPCC12N1/14C12P19/04
Inventor 傅俊生刘鑫侯若琳林文雄郑明锋
Owner 福建原培真牛樟芝生物科技有限公司
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