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Method for extracting vernonia glycoside D

A technology of vernin and extraction method, applied in the field of separation of natural products, to achieve the effects of increased yield, fast column passing, and low product loss

Inactive Publication Date: 2012-09-12
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the extraction of vernoside D from Vernonia amygdala

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take 1 kg of Vernonia almonds leaves, crush them, and take another 3 g of mixed enzymes, dissolve them in pH4 hydrochloric acid aqueous solution, add them to the raw materials and mix well, and perform enzymatic hydrolysis at room temperature for 36 hours to obtain the raw materials for enzymatic hydrolysis; put the raw materials for enzymatic hydrolysis into a microwave extraction tank, and add 10L of 70% Extract the ethanol solution twice, combine the two filtrates, filter through an 80-mesh filter, add the filtrate to a tubular composite membrane ultrafiltration with a molecular weight cut-off of 6000, collect the permeate, concentrate the filtrate under reduced pressure, add an appropriate amount of water to disperse to obtain a concentrated solution; concentrate Add water-saturated n-butanol to the liquid and stir evenly, pour it into a separatory funnel, collect the n-butanol layer after layering, extract twice, concentrate the extract to recover n-butanol, add a li...

Embodiment 2

[0024] Take 1 kg of Vernonia almonds leaves, crush them, take another 3 g of mixed enzyme, dissolve them in pH3 hydrochloric acid aqueous solution, add them to the raw materials and mix well, enzymatically hydrolyze at room temperature for 12 hours, and obtain the raw materials for enzymatic hydrolysis; put the raw materials for enzymatic hydrolysis into a microwave extraction tank, and add 5L of 70% The ethanol solution was extracted 3 times, the filtrates were combined three times, filtered through an 80-mesh filter, the filtrate was added to a tubular composite membrane ultrafiltration with a molecular weight cut-off of 6000, the permeate was collected, the filtrate was concentrated under reduced pressure, and an appropriate amount of water was added to disperse to obtain a concentrated solution; Add water-saturated n-butanol and stir evenly, pour it into a separatory funnel, collect the n-butanol layer after layering, extract twice, concentrate the extract to recover n-butan...

Embodiment 3

[0026] Take 5kg of leaves of Vernonia amygdala, crush them, take another 15g of mixed enzyme, dissolve them in pH 5 hydrochloric acid aqueous solution, add them to the raw materials and mix well, enzymatically hydrolyze at room temperature for 24 hours to obtain the raw materials for enzymatic hydrolysis; put the raw materials for enzymatic hydrolysis into a microwave extraction tank, add 40L70% Extract the ethanol solution twice, combine the two filtrates, filter through an 80-mesh filter, add the filtrate to a tubular composite membrane ultrafiltration with a molecular weight cut-off of 10,000, collect the permeate, concentrate the filtrate under reduced pressure, and add an appropriate amount of water to disperse to obtain a concentrated solution; concentrate Add water-saturated n-butanol to the liquid and stir evenly, pour it into a separatory funnel, collect the n-butanol layer after layering, extract 4 times, concentrate the extract to recover n-butanol, add a little ether...

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PUM

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Abstract

The invention relates to a method for extracting vernonia glycoside D. The vernonia glycoside D is purified by means of biological enzymolysis, membrane separation and aluminum oxide short-column purification, and product yield and content are high. The method includes the specific steps: crushing leaves of almond vernonia; adding 0.3% of biological enzyme for natural enzymolysis to obtain enzymolysis raw materials; adding the enzymolysis raw materials into 5-10 times of ethanol solution for performing microwave extraction for 2-3 times; adding extracting solution into an ultra-filtration membrane for filtration; depressurizing and concentrating filtrate; adding the concentrated solution into water-saturated normal butanol for performing extraction for 2-5 times; collecting normal butanol layer concentrating and recycling reagents; adding a few ether deposits into the concentrated solution; filtering the deposits; adding 95% of ethanol for reflux and dissolution; enabling dissolved solution to pass through an aluminum oxide activated carbon mixing short column; and depressurizing and drying column discharged solution to obtain the vernonia glycoside D. The method is simple in operation, low in energy consumption, less in pollution and suitable for industrial production.

Description

technical field [0001] The invention relates to the field of separation of natural products, in particular to a method for extracting vernoside D by enzymatic hydrolysis and membrane separation. Background technique [0002] Vernin D, white amorphous powder, belongs to steroidal saponins, CAS number: 171485-65-7, molecular formula: C 35 h 52 o 12 , molecular weight: 664.79, molecular structure formula: [0003] . [0004] Modern pharmacological studies have shown that: Vernin D has the effect of reducing weight. Adding Vernin D to mice for 14 days can significantly reduce the weight gain of mice and increase the excretion of mice. The liver weight and liver weight were found in autopsy. And the levels of cholesterol in plasma were significantly reduced. [0005] Almond Vernonia Vernonia amygdalina Del. is a plant of the Asteraceae dove genus, commonly known as bitter leaf, it is a small shrub that grows widely in tropical areas of Africa, and its leaves can be used ...

Claims

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Application Information

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IPC IPC(8): C12P33/20C07J17/00
CPCY02P20/10
Inventor 刘东锋吴艳波
Owner NANJING ZELANG MEDICAL TECH
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