Tissue Culture Rapid Propagation Seedling Method of Vernonia amygdala

Abstract

The invention discloses a tissue culture rapid-propagation seedling culture method of vernonia amygdalina, comprising the following steps: (1) collecting of an explant: collecting a tender stem, removing leaves, and flushing by using clean water, wherein the flushed stem is taken as the explant for standby application; (2) disinfecting of the explant: soaking and disinfecting the explant obtained in the step (1), and flushing by using sterile water; (3) primary culture: inoculating the disinfected explant obtained in the step (2) into an induction medium for culture; (4) subculture: separating and cutting adventitious buds formed in the induction medium in the step (3), and transferring into a subculture medium for culture; (5) rooting culture: selecting robustproliferated seedlings obtained in the step (4), and transferring into a rooting culture medium for culture; (6) transplanting: after root development is finished, washing off a base culture medium, exercising seedlings for 10 days, and then transplanting into a light matrix. The method has the advantages that the seedling propagation speed is fast, the production is not affected by seasons, the transplanting survival rate is high, the seedling consistency is good, and the like, and a technique is provided for large-scale standardized production of the vernonia amygdalina.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for rapid propagation of seedlings of Vernonia amygdala through tissue culture. Background technique [0002] Vernonia amygdalina Del., also known as Vernonia amygdalina, Vernonia apricot leaf, magic tree, bitter tree, South African tree, South African leaf, is a plant of the genus Vernonia in the family Asteraceae, native to Africa. Almond vernonia leaves are safe to eat and are used as a vegetable in places like Nigeria. Its leaves have a unique smell and bitterness, so they are often called bitter leaves. They can be used as medicine, and have great potential in treating tumors, especially breast cancer, and lowering blood pressure. [0003] Vernonia amygdala is widely used among folks in Southeast Asia and Taiwan, but it is relatively unknown in mainland China. In recent years, it has been introduced in Guangdong and Guangxi, but the number of female pa...

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Problems solved by technology

[0003] Vernonia amygdala is widely used by folks in Southeast Asia and Taiwan, but it is relatively unknown in mainland China. In recent years, it has been introduced in Guangdong and Guangxi, but the number of female parents and related application research are few.

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