Adventitious bud induction method for Juglans mandshurica Maxim.

A technology of juglans regia and explants is applied in the field of rapid propagation production of juglans regia seedlings, which can solve the problems of slow reproduction speed, degeneration and low multiplication coefficient, and achieve the effects of fast reproduction speed, time saving and high germination rate.

Inactive Publication Date: 2014-08-06
FORESTRY RES INST OF HEILONGJIANG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to long-term over-harvesting, its resources are close to extinction, which is far from meeting the needs of domestic and foreign market economic development and medical fields.
Under normal conditions, Juglans catalpa seeds are dormant, with slow reproduction speed, low efficiency and long cycle
[0003] Regarding the tissue culture of Juglans catalpa, due to the high pollution rate of the initial inoculation, the germination of explants is difficult, the browning and degeneration of in vitro cultures are serious, and the problems of low proliferation coefficient have not been well resolved, and it has not been widely used in production so far. application
Carry out asexual reproduction with dormant branches of Juglans catalpa (Zhou Yu et al. 2001, Zhang Jianying et al. 2011), the effect of asexual reproduction is not effective

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Selection of explants

[0022] Take mature Juglans catalpa seeds in August every year, wash with water, dry in the sun to remove the outer skin of Juglans catalpa, put them in a dry and ventilated place at a temperature of 20°C for later use, smash the shells with a hammer, and take the tip of Juglans catalpa seeds A zygotic embryo with a few cotyledons at the other end,

[0023] (2) Disinfection of zygotic embryos

[0024] First infiltrate with 70% ethanol for 30 seconds twice, and then use 0.1% mercury chloride HgCl 2 The solution was sterilized for 15 minutes, rinsed 5 times with sterile water, then soaked in sterile water for 2 hours, set aside,

[0025] (3) Pre-cultivation of zygotic embryos

[0026] After soaking in sterile water for 2 hours, the outer skin of the sterilized Juglans zygote embryos was removed, inoculated in the MS medium that added the hormone indole acetic acid IBA0.01mg / L and cultivated, sucrose 30g / L, agar 6g / L, The culture temperature ...

Embodiment 2

[0032] (1) Selection of explants

[0033] Take mature Juglans catalpa seeds every September, wash with water, dry in the sun to remove the outer skin of Juglans catalpa, put it in a dry and ventilated place at a temperature of 23°C for later use, smash the shell with a hammer, and take the tip of Juglans catalpa seeds A zygotic embryo with a few cotyledons at the other end,

[0034] (2) Disinfection of zygotic embryos

[0035] First infiltrate with 70% ethanol for 30 seconds twice, and then use 0.1% mercury chloride HgCl 2 The solution was sterilized for 15 minutes, rinsed with sterile water for 6 times, then soaked in sterile water for 3 hours, set aside,

[0036] (3) Pre-cultivation of zygotic embryos

[0037] After soaking in sterile water for 4 hours, the outer skin of the sterilized Juglans zygote embryos was removed, inoculated in the MS medium that added the hormone indole acetic acid IBA0.01mg / L and cultivated, sucrose 30g / L, agar 6g / L, The culture temperature is 2...

Embodiment 3

[0043] (1) Selection of explants

[0044] Take mature Juglans catalpa seeds every October, wash them with clean water, dry them in the sun to remove the outer skin of Juglans catalpa, put them in a dry and ventilated place at a temperature of 25°C for later use, smash the shells with a hammer, and take the tip of Juglans catalpa seeds A zygotic embryo with a few cotyledons at the other end,

[0045] (2) Disinfection of zygotic embryos

[0046] First infiltrate with 70% ethanol for 30 seconds twice, and then use 0.1% mercury chloride HgCl 2 The solution was sterilized for 15 minutes, rinsed with sterile water for 7 times, then soaked in sterile water for 4 hours, set aside,

[0047] (3) Pre-cultivation of zygotic embryos

[0048] After soaking in sterile water for 7 hours, the outer skin of the sterilized Juglans zygote embryos was removed, inoculated in the MS medium supplemented with hormone indole acetic acid IBA0.01mg / L and cultivated, sucrose 30g / L, agar 6g / L, The cult...

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PUM

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Abstract

The invention relates to an adventitious bud induction method for Juglans mandshurica Maxim.. The method comprises the following steps: (1) selecting an explant; (2) disinfecting a zygotic embryo; (3) pre-culturing the zygotic embryo; (4) selecting an adventitious bud explant; and (5) inducing and differentiating. The method is simple, convenient, rapid and effective, a large batch of tissue culture seedlings can be bred in a short time, a foundation can be laid for future culture of excellent clone plants, endangered trees in a crisis state in China can be protected and bred effectively, and a good foundation is laid for future further protection and utilization of the trees.

Description

technical field [0001] The invention relates to the rapid propagation production technology of Juglans catalpa seedlings in forestry biotechnology. Background technique [0002] At present, Juglans mandshurica Max im. belongs to the genus Juglans walnut, and is one of the three hard broad tree species in the Northeast forest area. Its material is hard and dense, and its texture is straight and beautiful. , shell and leaves can be used as medicine, widely used, high economic value. Due to long-term over-harvesting, its resources are nearly endangered, and it is far from meeting the needs of domestic and foreign market economy development and medical fields. Under normal conditions, the seeds of Juglans catalpa are dormant, and the reproduction speed is slow, the efficiency is low, and the cycle is long. [0003] Regarding the tissue culture of Juglans catalpa, due to the high pollution rate of the initial inoculation, the germination of explants is difficult, the browning a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张建瑛祁永会吕跃东刘建明邢亚娟田新华李京
Owner FORESTRY RES INST OF HEILONGJIANG PROVINCE
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