Method for improving yield of lucid ganoderma exopolysaccharides

An extracellular polysaccharide and Ganoderma lucidum cell technology is applied in the field of Vernonia almondii leaf powder to increase the yield of Ganoderma lucidum liquid fermentation exopolysaccharide, which can solve the problems of unstable quality of active ingredients, low production efficiency and the like, and achieve the enhancement of biological activity and pharmacological function. Effect

Active Publication Date: 2020-11-24
XINYANG NORMAL UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Traditionally cultivated Ganoderma lucidum is difficult to control due to environmental factors and growth ...

Method used

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  • Method for improving yield of lucid ganoderma exopolysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Adding 5g / L Almond Vernonia leaf powder to ferment and produce Ganoderma lucidum exopolysaccharide

[0018] Put the ganoderma lucidum mycelia stored on the PDA slope at 4°C at room temperature for 4 hours, then pick the mycelium with a size of 5mm×5mm on the slope and insert it into a fresh PDA plate, culture at 30°C for 7 days, the plate is covered with The mycelium is activated. Use a sterilized toothpick to pick out mycelium with a size of 5 mm × 5 mm, insert it into a 50 mL centrifuge tube containing 10 mL of seed medium, and culture it on a shaker at 28 ° C and 150 rpm for 7 days. In the ultra-clean workbench, next to the alcohol lamp, pour the well-cultivated seed culture solution (10ml per bottle) into a 250mL Erlenmeyer flask containing 100mL of fermentation medium added with Vernonia almond leaf powder. The addition amount of leaf powder is 5g / L. Place on a shaker at 28°C and 150 rpm for 8 days.

[0019] The obtained fermented liquid was transferre...

Embodiment 2

[0022] Example 2 Adding 10g / L Almond Vernonia leaf powder to ferment and produce Ganoderma lucidum exopolysaccharide

[0023] Other conditions were the same as in Example 1, except that the amount of Vernonia almond leaf powder was adjusted to 10 g / L. The result shows that the ganoderma mycelium biomass is 12.3g / L, and the ganoderma exopolysaccharide output is 3.32g / L (see figure 1 ).

Embodiment 3

[0024] Example 3 Adding 15g / L Almond Vernonia leaf powder to ferment and produce Ganoderma lucidum exopolysaccharide

[0025] Other conditions were the same as in Example 1, except that the addition of Vernonia almond leaf powder was adjusted to 15g / L. The results showed that the biomass of Ganoderma lucidum mycelium was 10.9g / L, and the yield of exopolysaccharide of Ganoderma lucidum was 3.04g / L.

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Abstract

The invention discloses a method for improving the yield of lucid ganoderma exopolysaccharides, and belongs to the field of microbial fermentation. In the method, vernonia amygdalina leaf powders areused for accelerating lucid ganoderma to ferment to produce the exopolysaccharides, and the yield of the lucid ganoderma exopolysaccharides is improved. The method discovers that if a proper quantityof vernonia amygdalina leaf powders is added into a liquid fermentation culture medium, ganoderma lucidum can be obviously accelerated to grow, ganoderma lucidum biomass is improved by 173.33%, in addition, the yield of the lucid ganoderma exopolysaccharides is improved by 205.71%, a new thought is provided for efficiently producing ganoderan, and the method is favorable for industrial production.

Description

technical field [0001] The invention belongs to the field of microbial fermentation and relates to liquid fermentation of ganoderma lucidum and preparation of ganoderma polysaccharides, in particular to a method for increasing the output of ganoderma lucidum liquid fermentation extracellular polysaccharides by using almond vernonia leaf powder. Background technique [0002] Ganoderma lucidum (Ganoderma lingzhi, formerly known as Ganoderma lucidum) is a rare edible and medicinal fungus with very high medicinal value. In ancient my country, its fruiting body was called "xiancao". "Pharmacopia of the People's Republic of China" has long ago officially included Ganoderma lucidum as a drug, and the Ministry of Health has also listed Ganoderma lucidum as a new type of food resource. At the same time, "American Herbal Pharmacopoeia" has officially included it as a herbal medicine. In recent years, the development and utilization of Ganoderma lucidum has attracted more and more peop...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12N1/38C12N1/14C12R1/645
CPCC12P19/04C12N1/38C12N1/14
Inventor 陈震毛光志王君一孔明慧韩冬叶灿辉
Owner XINYANG NORMAL UNIVERSITY
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