Tissue culture rapid-propagation seedling culture method of vernonia amygdalina

A technology for rapid breeding of Vernonia almonds and Vernonia seedlings, which is applied in the field of plant tissue culture, can solve the problems of the number of female parents and the lack of related application research, and achieve the effects of facilitating standardized production, conducive to preservation, and well-developed root system

Active Publication Date: 2016-06-15
钦州市林业科学研究所
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Benefits of technology

In this new technique called vernonaria amylacorum (VAM), there were several benefits compared to previous methods such as grafted seeds or immature roots that had limitations like slow growth due to temperature fluctuations over different periods of storage. However, it still showed strong resistance towards bacterial diseases caused by microorganisms found within soil samples collected from field sites after harvesting. Therefore, an improved version of these techniques could help improve their effectiveness at producing commercially viable crops.

Problems solved by technology

The technical problem addressed by this patented research relates how to create an improved method or technique that allows for quicker growth rates during cultivating favaoms such as those found naturally throughout North America (Vanilla Amytrichla).

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  • Tissue culture rapid-propagation seedling culture method of vernonia amygdalina

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Embodiment Construction

[0029] Below with embodiment the present invention will be further described, but the present invention is not limited to these implementations

[0030] example. Example:

[0031] The tissue culture rapid propagation seedling method of Vernonia amygdala comprises the following steps:

[0032] 1) Collection of explants: select healthy and healthy plants without damage from diseases and insect pests, and cut the young shoots with less lignification degree as explants;

[0033] 2) Disinfection of explants: Cut the harvested stem section into a 1.2-1.5cm section with joints, cut off the leaves, and only keep the base of the petiole (about 0.5cm in length), rinse with running water for 20 minutes for later use; put it on the ultra-clean workbench , soaked in 75% alcohol for 10s, 0.1% mercuric chloride oscillating disinfection for 8min, rinsed with sterile water for 5 times to wash off the residual medicinal solution, blotted the water with sterile filter paper, cut off the two en...

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Abstract

The invention discloses a tissue culture rapid-propagation seedling culture method of vernonia amygdalina, comprising the following steps: (1) collecting of an explant: collecting a tender stem, removing leaves, and flushing by using clean water, wherein the flushed stem is taken as the explant for standby application; (2) disinfecting of the explant: soaking and disinfecting the explant obtained in the step (1), and flushing by using sterile water; (3) primary culture: inoculating the disinfected explant obtained in the step (2) into an induction medium for culture; (4) subculture: separating and cutting adventitious buds formed in the induction medium in the step (3), and transferring into a subculture medium for culture; (5) rooting culture: selecting robustproliferated seedlings obtained in the step (4), and transferring into a rooting culture medium for culture; (6) transplanting: after root development is finished, washing off a base culture medium, exercising seedlings for 10 days, and then transplanting into a light matrix. The method has the advantages that the seedling propagation speed is fast, the production is not affected by seasons, the transplanting survival rate is high, the seedling consistency is good, and the like, and a technique is provided for large-scale standardized production of the vernonia amygdalina.

Description

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Claims

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Application Information

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Owner 钦州市林业科学研究所
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