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Preparation method and application of vernonia amygdalina saponin

A technology of almond vernonia saponin and almond leaf spot, applied in the field of natural medicinal chemistry, can solve the problems of large consumption of organic solvents, difficulty in mass production, etc., and achieve the effect of improving purity and reducing burden

Active Publication Date: 2018-12-18
嘉文丽(福建)化妆品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above two methods are only suitable for laboratory preparation and are difficult for mass production
Patent CN201210115917.7 discloses a method for extracting and separating Vernonia saponin D. The method includes enzymatic hydrolysis, extraction, membrane filtration, extraction, alumina column chromatography and other processes. The process is long and consumes a large amount of organic solvents. Difficult to mass-produce, and the yield is only 0.286%

Method used

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  • Preparation method and application of vernonia amygdalina saponin
  • Preparation method and application of vernonia amygdalina saponin
  • Preparation method and application of vernonia amygdalina saponin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1: the preparation of almond vernonia total saponins, such as figure 1 :

[0051] (1) extraction

[0052] Take 1 kg of dried Vernonia amygdala leaves, crush them, and extract them with 3 times 40% alcohol at a temperature of 60°C for 1.5 hours; filter and collect the filtrate. Leach twice to obtain a total of about 5L of extract.

[0053] (2) Flocculation

[0054] Heat the extract so that its temperature is 60°C, add 24mL 5% polyaluminum chloride solution to the extract, stir and mix quickly; then add 300mL 1% chitosan solution to the solution, stir and mix quickly, and finally use 5% Sodium carbonate solution was used to adjust the pH to 6; after standing for 2 hours, it was filtered and the filtrate was collected.

[0055] (3) refined

[0056] The above-mentioned filtrate is passed through the AB-8 macroporous adsorption resin column, and the control flow rate is 2-3BV / h; then use 1.5BV containing 25% alcohol (containing 0.3% sodium carbonate) to elute t...

Embodiment 2

[0059] Embodiment 2: Preparation of total saponins of Vernonia amygdala

[0060] (1) extraction

[0061] Take 500g of dried Vernonia amygdala leaves, crush them, extract with 4 times of 30% alcohol, the extraction temperature is 65°C, and the extraction time is 2 hours; filter and collect the filtrate. Leaching twice, a total of about 3.5L of extract was obtained.

[0062] (2) Flocculation

[0063] Heat the extract to 60°C, add 21mL of 5% polyferric chloride solution, stir and mix quickly, and filter; then add 350mL of 0.5% polyacrylamide solution to the filtrate, stir and mix quickly, and finally add 5% sodium carbonate The pH of the solution was adjusted to 6; after standing for 2 hours, it was filtered and the filtrate was collected.

[0064] (3) refined

[0065] The above-mentioned filtrate is passed through the AB-8 macroporous adsorption resin column, and the control flow rate is 2-3BV / h; then use 1.5BV containing 30% alcohol (containing 0.2% sodium carbonate) to elu...

Embodiment 3

[0068] Example 3 Antibacterial and anti-acne essence preparation

[0069] Phase A: 2.0g total vernonia saponins, 10.0g butanediol, 1.0g tea tree oil, 0.5g Tween-80 phenoxyethanol 0.05g; phase B: 80ml water, 20.0g 1% hyaluronic acid, 5.0g glycerin and mix well ; Phase A and Phase B are heated and dissolved first, and then the two are mixed and stirred to obtain the antibacterial and anti-inflammatory essence.

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Abstract

The invention belongs to the technical field of natural medicine chemistry, relates to the technical field of extraction and separation of natural plant active components and particularly relates to apreparation method and application of vernonia amygdalina saponin. Vernonia amygdalina is also named as bitter leaf or South Africa leaf and has the effects of expelling parasite, resisting malaria and promoting excretion. The research shows the saponin separated from vernonia amygdalina has the functions of resisting malaria, relieving pain and relieving fever and is regarded as a main active component of vernonia amygdalina. The invention discloses an industrial extraction and separation process of vernonia amygdalina total saponin; through adoption of the process, the yield of the vernoniaamygdalina total saponin is about 4.2%; the content is greater than 90%.

Description

technical field [0001] The invention belongs to the technical field of natural medicinal chemistry, and relates to extraction and separation technology of natural plant active ingredients, in particular to a preparation method and application of almond vernonia saponin. Background technique [0002] Vernonia amygdalina Del., also known as bitter leaf and South African leaf, is a plant of the genus Vernonia (Asteraceae) that grows in tropical areas of Africa. In West Africa, especially in Nigeria, the locals call it "Bitter-leaf", and use its leaves to make a very popular local bitter leaf soup, which has the functions of repelling insects, anti-malaria, and diarrhea. Vernonia amygdala contains a variety of bioactive chemical components, which mainly include saponins, alkaloids, terpenes, steroids, coumarins, flavonoids, phenolic acids, lignans, xanthones, anthraquinones and Semiterpene etc. [1] . According to literature [2-3] It is reported that the saponins isolated fro...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61P17/10A61P31/02A61Q17/00A61Q19/00
CPCA61K2800/805A61Q17/005A61Q19/00A61K8/9789A61P17/10A61P31/02Y02A50/30
Inventor 饶焕文林建平林晓峰萧自智潘发伍
Owner 嘉文丽(福建)化妆品有限公司
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