Non-test tube rapid propagation method for Japanese red maple seedling
A test-tube and rapid technology, applied in horticultural methods, botanical equipment and methods, fertilizer mixtures, etc., can solve the problems of high cost and difficulty in seedling cultivation, and achieve the advantages of shortened rooting time, fast reproduction, and accelerated propagation. Effect
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[0016] a. Establish greenhouses and seedbeds
[0017] A greenhouse simulating the microenvironment of tissue culture test tubes is composed of an arch 3, a mist spray nozzle 4 for spraying water to cool down, a supplementary light 5, and a water pipe 8 with a timer 6 and a solenoid valve 7. The arch frame is covered with micro-membrane to form the whole arch shed, and the height of the arch shed is 70 cm. A full-angle fill light 5 is added to the shed, and the light saturation point is 40000 Lux. The seedbed is composed of a special seedling tray 1 for forest trees and a perlite matrix 2 which are convenient for drainage. The seedbed is 1 meter wide, 3 meters long and 0.7 meters high.
[0018] b. Preparation of isolated materials and inorganic nutrient solution
[0019] The 4-6cm leafy plant section or one leaf and one bud of the Japanese red maple's semi-lignified young branches in the same year is used as the isolated material, sterilized, the morphological upper cut is s...
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