Shoot cutting seedling-culturing method of cyclocarya paliurus

Abstract

A shoot cutting seedling-culturing method of cyclocarya paliurus mainly comprises the steps of processing of a shoot mother tree, preparation and processing of a cutting medium, processing of auxin, trimming and cutting of a shoot, management after cutting, transplantation and the like. The new seedling-culturing method through the new medium and a new hormone recipe resolves the problems that cutting callus tissue of cyclocarya paliurus is difficult to form, organ differentiation is difficult, root growth time is long and root growth rate is low, is a method for developing and utilizing the plant resources of cyclocarya paliurus protectively, and provides powerful technical guarantee for reutilization of germplasm resources.

Description

technical field

[0001] The invention relates to the asexual reproduction technology of plants, in particular to a method for growing seedlings by cuttings of Cyclocarya paliurus twigs. Background technique

[0002] According to relevant information, Cyclocarya paliurus is a rare tree species that survived the Quaternary Glacier and belongs to the national second-class protected tree species. It is a unique natural health food resource in my country. It is found in many provinces in southern China, mostly in sporadic distribution. It is generally believed that Cyclocarya paliurus is rich in various mineral elements related to human health and hypoglycemic effect, and its roots, branches, and leaves can be used as medicine. With a history of more than 100 years, this health-care tea has a delicate fragrance and taste as sweet as licorice, so it is called "sweet tea tree". All in all, Cyclocarya paliurus is a health food resource that integrates multiple unique functions such ...

Examples

Embodiment 1

[0022] In the nursery of an experimental forest farm in northern Hunan, the cuttings of Cyclocarya paliurus twigs were raised. First, in January of that year, the mother tree was cut to a height of 15 cm. Then, the cutting substrate was treated in the seedling tray. In the example, the cutting substrate is a mixture of peat soil with a particle size of 1~2mm and perlite at a ratio of 2:1. The hole diameter is 6cm and the depth is 11cm; Cuttings with a degree of lignification, and keep the length of each cutting between 8 and 10 cm, and contain 2 axillary buds, and keep 2 and a half leaves, sterilize the cuttings with a 1000-fold concentration of chlorothalonil solution, and then sterilize the base of the cuttings Dip in mud and soak in 600ppm IBA auxin solution for 60 minutes; then carry out cuttings once in early June and mid-August, and the cutting amount is 1:1. Insert the germinated branches into the small hole, the depth is about 5cm, and press it properly. Then drenched...

Embodiment 2

[0025] In an ecological agricultural garden in Suining County, Hunan Province, the cuttings of Cyclocarya paliurus twigs were raised. First, in January of that year, the mother tree was cut off to a height of 20 cm; In this embodiment, the cutting substrate is the mixed soil quality after peat soil and perlite with a particle size of 1-2 mm are mixed in a ratio of 2:1, and the diameter of the hole mouth is 6 cm and the depth is 11 cm; Select semi-lignified cuttings, keep the length of each cutting between 8 and 10 cm, and contain 2 axillary buds, and keep 2 and a half leaves, sterilize the cuttings with a 1000-fold concentration of chlorothalonil solution, and then sterilize the cuttings Soak the base in 600ppm IBA auxin solution with mud for 60 minutes; then cuttages were carried out in early June and mid-August, and the cutting amount was 1:1. When cutting, first insert a small hole in the matrix, and then put Insert the treated sprouted branches into the small hole with a d...

technical field

[0001] The invention relates to the asexual reproduction technology of plants, in particular to a method for growing seedlings by cuttings of Cyclocarya paliurus twigs. Background technique

[0002] According to relevant information, Cyclocarya paliurus is a rare tree species that survived the Quaternary Glacier and belongs to the national second-class protected tree species. It is a unique natural health food resource in my country. It is found in many provinces in southern China, mostly in sporadic distribution. It is generally believed that Cyclocarya paliurus is rich in various mineral elements related to human health and hypoglycemic effect, and its roots, branches, and leaves can be used as medicine. With a history of more than 100 years, this health-care tea has a delicate fragrance and taste as sweet as licorice, so it is called "sweet tea tree". All in all, Cyclocarya paliurus is a health food resource that integrates multiple unique functions such ...

Examples

Embodiment 1

[0022] In the nursery of an experimental forest farm in northern Hunan, the cuttings of Cyclocarya paliurus twigs were raised. First, in January of that year, the mother tree was cut to a height of 15 cm. Then, the cutting substrate was treated in the seedling tray. In the example, the cutting substrate is a mixture of peat soil with a particle size of 1~2mm and perlite at a ratio of 2:1. The hole diameter is 6cm and the depth is 11cm; Cuttings with a degree of lignification, and keep the length of each cutting between 8 and 10 cm, and contain 2 axillary buds, and keep 2 and a half leaves, sterilize the cuttings with a 1000-fold concentration of chlorothalonil solution, and then sterilize the base of the cuttings Dip in mud and soak in 600ppm IBA auxin solution for 60 minutes; then carry out cuttings once in early June and mid-August, and the cutting amount is 1:1. Insert the germinated branches into the small hole, the depth is about 5cm, and press it properly. Then drenched...

Embodiment 2

[0025] In an ecological agricultural garden in Suining County, Hunan Province, the cuttings of Cyclocarya paliurus twigs were raised. First, in January of that year, the mother tree was cut off to a height of 20 cm; In this embodiment, the cutting substrate is the mixed soil quality after peat soil and perlite with a particle size of 1-2 mm are mixed in a ratio of 2:1, and the diameter of the hole mouth is 6 cm and the depth is 11 cm; Select semi-lignified cuttings, keep the length of each cutting between 8 and 10 cm, and contain 2 axillary buds, and keep 2 and a half leaves, sterilize the cuttings with a 1000-fold concentration of chlorothalonil solution, and then sterilize the cuttings Soak the base in 600ppm IBA auxin solution with mud for 60 minutes; then cuttages were carried out in early June and mid-August, and the cutting amount was 1:1. When cutting, first insert a small hole in the matrix, and then put Insert the treated sprouted branches into the small hole with a d...