77 results about "Melaleuca alternifolia" patented technology

The invention relates to the technical field of plant cultivation, in particular to a phoebe bournei compound management method for cultivating long phoebe bournei with short phoebe bournei, which comprises the following steps: removing all plants on a forest land, and marking a management mode of a planting belt as follows: at least one line of phoebe bournei and one line of melaleuca alternifolia are interplanted, the line spacing of phoebe bournei is 2-3m * 3-7m, and the plant line spacing of melaleuca alternifolia is 1-3m * 1-3m; after phoebe bournei seedlings are subjected to seedling strengthening treatment, the phoebe bournei seedlings and melaleuca alternifolia are subjected to afforestation together, the melaleuca alternifolia are subjected to stubble cutting from January to February of the second year of afforestation and field planting, the stubble height is 10-35 cm, branches and leaves are taken to extract melaleuca alternifolia essential oil, waste generated through extraction and animal waste are mixed according to the ratio of 1: (1-2) for composting, organic fertilizer is obtained, and the organic fertilizer is applied in winter. Compared with the prior art, after the phoebe bournei is subjected to seedling strengthening treatment, the phoebe bournei is interplanted with the melaleuca alternifolia, the plant height, the ground diameter and the maximum crown breadth of the phoebe bournei are increased, and the economic benefit of a man-made forest is increased by means of short cultivation and long cultivation.

The invention discloses a preparation process of a pure plant essential oil disinfectant, and relates to the technical field of disinfectants. The problems that plant essential oil is poor in solubility in a water phase, low in stability and the like are solved. The method specifically comprises the following steps: preparing raw materials; preparing a nano emulsion; preparing a solvent and mixing; constructing a natural stable system to obtain a primary product; filtering and sterilizing; and carrying out sterile filling on the disinfectant finished product. The invention provides a pure plant essential oil disinfectant prepared by dissolving type 1 and 8 melaleuca alternifolia essential oil in water, efficient dispersion is realized through a nano-emulsification and natural stabilization technology so as to form a stable aqueous solution, an amphiphilic structure of soapbark saponin is utilized, aglycone and essential oil terpene form a supramolecular compound, and the stable aqueous solution can be used for sterilizing the melaleuca alternifolia essential oil and the melaleuca alternifolia essential oil and the melaleuca alternifolia essential oil. The technical problems of poor dissolvability and low stability of plant essential oil in a water phase are solved, and the plant essential oil has broad-spectrum antibacterial and antiviral characteristics and can meet the disinfection requirements in multiple fields such as medical treatment, object surface treatment, home furnishing and personal care.

The invention belongs to the field of agricultural technology and ecological monitoring, and particularly discloses a Hainan island wild tea tree growth model construction method and system.The method comprises the steps that firstly, a microenvironment competition factor is obtained by quantifying a local competition index based on diameter-level distribution in a fixed quadrat around a target tea tree; selecting a preset cubic function growth mechanism model to calculate a theoretical growth index value according to a vegetation vertical zone corresponding to the altitude of the tea tree; then fusing the local competition index, the theoretical growth index value, the altitude, the canopy density and the growth environment type to construct a feature vector so as to train a gradient lifting decision tree model, and establishing a multi-factor growth state prediction model; and finally, predicting the growth state of an unknown tea tree by using the model. According to the method, the ecological mechanism and machine learning are effectively fused, the growth prediction precision is remarkably improved, and a quantitative basis is provided for precise conservation.

The invention provides a GC-MS (Gas Chromatography-Mass Spectrometer) quality detection method for a pulse-invigorating and blood-glucose-reducing traditional Chinese medicine composition. The detection method has the advantages of simple and convenient operation steps, accurate detection and high stability. According to the preparation method, volatile components in the blood-glucose-promoting and blood-glucose-reducing granules are determined, and 15 batches of content determination shows that the content of atractylenol in the blood-glucose-promoting and blood-glucose-reducing granules is 0.0205 to 0.1893 mg / g, the content of beta-eudesmol in the blood-glucose-promoting and blood-glucose-reducing granules is 0.0643 to 0.5737 mg / g, the content of palmitic acid in the blood-glucose-promoting and blood-glucose-reducing granules is 0.7319 to 5.1163 mg / g, the content of ferruginol in the blood-glucose-promoting and blood-glucose-reducing granules is 0.0743 to 0.2356 mg / g, and the content of tanshinone IIA in the blood- The detection method can be used as an internal control quality detection standard of the product to further guarantee the product quality. And the detection method can also be used for identifying the chemical components of the traditional Chinese medicine composition for dredging collaterals and reducing blood glucose, and has a good market prospect.

The invention relates to the technical field of plant genetic engineering, in particular to a transcription factor CsKNAT1 and application thereof in regulation and control of development of tea trees. The nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the sequence of protein coded by the gene is as shown in SEQ ID NO: 2. Research finds that overexpression of the CsKNAT1 gene can influence the form of plant leaves: the edges of the leaves are promoted to have notches (asymmetric leaves are formed, resulting in increase of sawteeth at the edges of the leaves); the formation of compound leaves can be promoted; leaves on plant stems can grow in a rosette form, and secondary stems are bolted in the middles of rosette leaves; the research also finds that the overexpression of the CsKNAT1 gene can obviously influence the flowering phase and the flower form of the plant; the delay of the flowering phase is promoted; overexpression of the CsKNAT1 gene can further influence development of floral organs and formation of siliques, part of arabidopsis plants have phenotypes that corolla becomes small, stamens are exposed, petals gradually extend and are zigzag in a wavy shape, the length of mature siliques is remarkably shortened by 20%-36.1%, and the phenomenon that the fruits are aborted occurs.

The invention discloses a method for preparing a humic acid water-soluble fertilizer from caragana microphylla slurry and melaleuca alternifolia essential oil, and relates to the technical field of fertilizers. The problems that raw material resources for preparing the humic acid water-soluble fertilizer are limited, and the preparation cost is high are solved. The method specifically comprises the following steps: preparing raw materials: caragana microphylla, melaleuca alternifolia essential oil and an auxiliary reagent; preparation and biotransformation of caragana microphylla pulp; emulsifying the melaleuca alternifolia essential oil; performing fertilizer compounding and quality regulation and control; and processing and storing finished products. The method has the characteristics of efficient utilization of resources, function synergy and environmental friendliness, the caragana microphylla in the preparation raw materials is a waste branch in a desert area and can turn waste into wealth, the essential oil is a natural extract and replaces a chemical synthesis synergist in an existing preparation method, soil is improved through humic acid, soil-borne diseases are inhibited through the essential oil, root absorption is promoted through the essential oil, and the soil-borne diseases are prevented from being damaged. Meanwhile, the humic acid water-soluble fertilizer is free of harmful residues and suitable for green agriculture.

The present invention relates to an antimicrobial and healing formulation for skin, designed to effectively target and treat skin conditions such as acne caused by Cutibacterium acnes, cold sore lesions due to the herpes simplex virus, and superficial wounds. This unique formulation combines antimicrobial agents, plant extracts and essential oils in specific proportions in order to exert a synergistic action, enhancing their individual efficacy in the treatment of skin conditions and promoting skin healing. The innovative formulation composed of niaouli (Melaleuca viridiflora) essential oil and tea tree (Melaleuca alternifolia) essential oil, chlorhexidine digluconate, Hamamelis virginiana extract, panthenol, glycerol, and propylene glycol. Each ingredient has been selected for its proven pharmacological properties and its synergistic potential. The formulation works in concert to reduce microbial load, alleviate inflammation, and accelerate skin healing, while providing a gentle and effective application.

The invention discloses application of a CsNOMT gene and sakuranetin in prevention and treatment of tea tree anthracnose. It is found for the first time that methyltransferase encoded by the tea tree-derived CsNOMT gene can efficiently catalyze naringenin to be converted into sakuranetin, the endogenous phytoalexin level of a tea tree is remarkably improved, and then the disease resistance of the tea tree-derived CsNOMT gene to Colletotrichum carolinae is enhanced. The silence of the CsNOMT gene can reduce the sakuranetin content by 30-40%, resulting in the increase of the scab area; and by overexpressing the gene, the sakuranetin content is increased by 30%, and the scab area is remarkably reduced. In addition, the growth of colletotrichum can be effectively inhibited by exogenously spraying 0.05-0.3 mM of sakuranetin, and the optimal concentration is 0.1-0.3 mM. The invention provides a new strategy for green prevention and control of tea tree anthracnose, and has the characteristics of high efficiency, safety and environmental friendliness.

The invention discloses a gene for degrading chlorophyll as well as an encoding protein and application thereof, and belongs to the fields of molecular biology, plant genetic engineering and horticulture. Protein coded by the gene is located in chloroplast, and chlorophyll degradation can be efficiently promoted under various stress conditions. By performing different treatments on Huangkui tea trees and performing gene function verification in model plants such as nicotiana benthamiana and arabidopsis thaliana, it is found that the CsSGRs gene is subjected to induced expression by factors such as natural aging, abscisic acid, illumination intensity and low temperature, and the gene participates in a chlorophyll degradation approach, so that the functions are redundant. In addition, a bZIP family transcription factor CsABF4 is screened out, and CsSGRs gene expression can be regulated and controlled. The invention provides gene resources and theoretical basis for regulating and controlling the chlorophyll content of plants and improving related characters of plant leaf color, and has potential application value in the fields of plant breeding and gardening.

The application relates to the technical field of genetic engineering, in particular to application of CsCAT1 in regulating distribution of theanine between new shoots and leaves of tea trees, and to reduction of the stem / leaf ratio of theanine in tea trees by reducing the expression amount of CsCAT1 in tender stems, wherein the base sequence of the CsCAT1 is shown as SEQ ID NO. 1, the stem / leaf ratio of theanine in the tea trees is reduced, the content of theanine in tender leaves of the tea trees is increased, and the quality of tea leaves is further improved.

The invention discloses a composite operation mode and method for improving interplanting melaleuca alternifolia after intermediate cutting of eucalyptus, and the method comprises the following multiple key steps: forest stand selection: selecting a hybrid eucalyptus clone forest stand of more than 4 years old; during intermediate cutting and soil preparation, strip-shaped intermediate cutting is carried out along a contour line, soil is improved in a targeted mode, and a composite modifier containing a functional microbial agent and the like is applied; interplanting: selecting high-quality container seedlings, and standardizing field planting to guarantee the survival rate; fertilization management is carried out according to the growth stages of eucalyptus and melaleuca alternifolia, a special slow release fertilizer is researched and developed, and nutrient requirements are accurately matched; through differentiated nutrient design, functional microorganism cooperation and slow release technology regulation and control, plant growth is effectively supported, essential oil quality and forest stand productivity are improved, short-term income and long-term operation are balanced, rhizosphere microenvironment is improved, chemical input negative effects are reduced, the advantages of the method in efficient utilization of resources and ecological sustainability are verified, and the method is worthy of popularization and application. A feasible technical scheme is provided for eucalyptus compound management, and the method has popularization and application values.

The invention discloses application of a KASP molecular marker in identification of resistance to physalospora piricola of tea trees, and belongs to the technical field of plant molecular biology. The invention aims to develop the KASP molecular marker closely linked with the resistance of the tea tree physalospora piricola and accelerate the process of assisted breeding of the molecular marker with the resistance of the tea tree physalospora piricola. The KASP marker for identifying the resistance to the physalospora piricola of the tea tree is Cs-KASP, and the marker is positioned to an SNP related to identification of the resistance to the physalospora piricola of the tea tree through generic genome sequence alignment. A group of KASP molecular markers closely linked with the resistance of the tea tree physalospora piricola are developed according to the SNP site. The marker can be used for genotype detection of different resistant materials of the tea tree physalospora piricola, can be used for successfully distinguishing a disease-resistant material from a disease-susceptible material, and is combined with a high-throughput genotyping detection system to quickly identify physalospora piricola-resistant germplasm resources, so that the breeding efficiency is improved.

The present inventive concept relates to a formula for vaginal and anal cleansing and bacterial and microbial treatment of the same. The formula consists of a combination of Nano or Colloidal Siver, Melaleuca alternifolia which is Tea Tree and either distilled water or purified water. The formula is preferably applied through an applicator bottle or douche bottle.

The application discloses a method for identifying original tea tree varieties by using characteristic metabolites, and particularly relates to the field of identifying tea tree varieties, which comprises the steps of sample preparation, metabolite extraction, liquid chromatography-mass spectrometry detection, data correction processing, characteristic metabolite construction and multivariate statistical clustering determination, etc.; in view of the internal metabolic characteristics formed in the long-term natural domestication process of the floating Liang chestnut leaf population, a high-dimensional characteristic metabolite vector is constructed by using the stable numerical distribution mode of the fresh leaves of the floating Liang chestnut on the sixteen characteristic metabolites; after the peak intensity data of each sample is corrected, filtered and structured, the obtained characteristic vector is input into the Euclidean distance hierarchical clustering model, so that the metabolic composition difference between the samples to be identified and the standard floating Liang chestnut leaf population is unsupervised statistically distinguished.

The invention discloses a makeup cosmetic and a production method thereof, and the cosmetic is composed of a natural functional composition, natural grease and wax, natural powder and a coloring agent, a natural emulsified stable system, a natural humectant and purified water, the natural effect composition is formed by scientifically compounding melaleuca alternifolia extract, salvia miltiorrhiza root extract, witch hazel extract, aloe barbadensis leaf juice powder and amur corktree bark extract. The production method comprises the following steps: preparing an oil phase and a water phase, emulsifying, adding effect and color components, degassing, filling, cooling and shaping. The product can effectively inhibit harmful microorganisms on the skin, regulate grease and prevent acnes while providing excellent concealing and moisturizing makeup effects, the whole formula is natural, safe and environment-friendly, multifunctional integration of makeup and maintenance is realized, and the product is particularly suitable for oily, acne and sensitive skin.

The invention relates to the technical field of plant essential oil extraction, and discloses natural acne-removing repair essential oil which is prepared from the following raw materials in percentage by weight: 18-22% of eucalyptus essential oil, 18-22% of cananga odorata essential oil, 18-22% of lavender essential oil, 17-21% of juniper essential oil, 16-20% of cajeput essential oil, 0.5-1.5% of cuba balsam essential oil, 0.5-1.5% of Somasum essential oil and 0.5-1.5% of Roman chamomile essential oil. Through synergistic combination of eucalyptus essential oil, cananga odorata essential oil, lavender essential oil, juniper essential oil, cajeput essential oil, cuba balsam essential oil, Somarie frankincense essential oil and Roman chamomile essential oil, acne removal and repair characteristic integration of the essential oil components is guaranteed, so that the overall efficacy is enhanced.

The present invention relates to a non-therapeutic cosmetic composition for increasing the cellular mitochondrial respiration of keratinocytes underlying the stratum corneum of the epidermis, comprising an active complex: extracts of Melaleuca alternifolia, extracts of Lavandula hybrida, and melatonin. The invention also relates to the use of this non-therapeutic cosmetic composition for increasing the cellular mitochondrial respiration of keratinocytes underlying the stratum corneum of the epidermis.

The present invention discloses an agricultural composition for controlling strawberry gray mold, belonging to the technical field of agricultural compositions. The agricultural composition provided by the present invention comprises resveratrol and a Melaleuca alternifolia extract as active ingredients, with the mass ratio of resveratrol to the Melaleuca alternifolia extract being 1:20 to 5:1. The present invention combines resveratrol and the Melaleuca alternifolia extract for controlling strawberry gray mold, exhibiting a synergistic effect.

The invention discloses a microbial agent for improving disease resistance of tea trees and a preparation method of the microbial agent, and belongs to the field of agricultural disease prevention and control. According to the invention, the optimal strain compounding condition is obtained by screening the strain compounding condition, and experiments prove that the fungicide prepared when the brevibacterium Hainan powder ACCC 60371 and the streptomyces griseus var rust CICC 23624 are compounded according to the mass ratio of 2: 5 can enable the prevention and treatment effects of tea leaf blight and zonate spot to reach 88.58% and 84.41% respectively, so that the fungicide can be used for preventing and treating the tea leaf blight and zonate spot respectively. And the polyphenol content is obviously superior to the effect of preventing and treating the two tea tree diseases by single bacteria. The invention provides a strain source and a method for improving disease resistance and tea quality.

The invention discloses antibacterial tinea-removing foot bath cream containing cajeput flavone extract and a preparation method of the antibacterial tinea-removing foot bath cream, and relates to the technical field of daily chemical products and traditional Chinese medicine preparations. The foot bath cream is prepared from the following components in percentage by weight: 5 to 10 percent of cajeput flavone extract, 1 to 3 percent of radix sophorae flavescentis extract, 0.5 to 1.5 percent of tea tree essential oil, 5 to 8 percent of cutin softening penetration enhancer, 1 to 3 percent of hidroschesis astringent, 15 to 25 percent of emulsified grease matrix, 8 to 12 percent of humectant, 0.1 to 0.3 percent of preservative, 0.2 to 0.5 percent of thickening stabilizer and the balance of water. The preparation method of the foot bath cream comprises the following steps: (1) preparing an oil phase; (2) preparing a water phase; (3) emulsifying and homogenizing; (4) adding components; (5) blending flavor and preventing corrosion; and (6) aging and filling. The cajeput flavone extract with good water solubility and high stability is adopted as a main component and is matched with the cutin softening penetration enhancer and other natural antibacterial components to construct a multi-component synergistic effect system, so that the product has the characteristics of naturalness, safety, mildness, durability and high efficiency.

The application discloses application of a DsHAD gene in regulating growth, reproduction, pathogenicity and adversity resistance of Didymella segeticola. The DsHAD gene is derived from the Didymella segeticola, a knockout gene fragment of the DsHAD gene of D.segeticola is constructed, and the knockout mutant is obtained by introducing the knockout gene fragment into D.segeticola protoplast. It is found through experiments that the DsHAD gene has an effect on the growth, reproduction and adversity resistance of D.segeticola, and significantly reduces the pathogenicity of D.segeticola to tea tree leaves. Therefore, the DsHAD can be used as a fungicide target and a key protein of a pathogenic mechanism of a disease, and is used for developing a medicament for the disease caused by D.segeticola infection and cultivating a new variety of disease-resistant plants, and has a wide application prospect in the prevention and treatment of plant pathogenic fungal diseases.

The invention discloses an application of a D. segeticola ethanol dehydrogenase (DsADH) gene in the regulation of the growth, the reproduction, the pathogenicity and the stress resistance of D. segeticola, and particularly discloses a D. segeticola ethanol dehydrogenase (DsADH) gene in the regulation of the growth, the reproduction, the pathogenicity and the stress resistance of D. segeticola. The DsADH gene disclosed by the invention is derived from a pathogenic bacterium D.segeticola, and a DsADH gene knockout gene segment is constructed and is introduced into a protoplast of the D.segeticola, so that a DsADH gene knockout mutant is obtained. Researches find that the DsADH gene plays an important role in regulating the growth and reproduction of D.segeticola and influencing osmotic stress and oxygen stress capabilities such as high salinity, and obviously reduces the pathogenicity of D.segeticola hyphae to tea tree leaves. Therefore, the DsADH can be used as a key gene for researching a disease pathogenic mechanism and a target of a bactericide, so that the bactericide for preventing and treating D.segeticola is researched and developed, or the DsADH is applied to gene function research of germs, a new variety for resisting plant diseases is favorably cultivated, and the DsADH has a wide application prospect in plant fungal disease research.

The invention discloses a tea tree stem end callus browning prevention and adventitious bud induction technology, and relates to the technical field of plant tissue culture. The method comprises the following steps: treatment and induction of an explant: taking a tender stem of a tea tree as the explant, sterilizing, inoculating into a first culture medium, culturing, and inducing a callus; subculture: transferring the callus obtained in the step S1 into a second culture medium for subculture to promote proliferation of the callus; adventitious bud induction: transferring the callus subjected to secondary subculture into a third culture medium for culture, and inducing adventitious buds; wherein the first culture medium and the second culture medium both contain activated carbon and melatonin; the third culture medium contains 2-IP. According to the method disclosed by the invention, an activated carbon and melatonin synergistic anti-browning system is innovatively adopted, so that the average browning rate of the calluses of the tea trees is reduced to 15.90%-16.47%; 2-IP is applied to adventitious bud induction of tea tree callus for the first time, and the adventitious bud induction rate and growth quality are greatly improved.

A topical formulation, comprising: an ointment base; and a mixture of active pharmaceutical ingredients (APIs) contained in the ointment base, the mixture of APIs consisting of: Metronidazole; Ivermectin; and Melaleuca alternifolia (tea tree) oil. The APIs are about 0.12%-6% w / w and the ointment base is about 94% to 99% w / w of the topical formulation. The formulation can further include enhancers for the APIs, such as, moisturizers and anti-oxidants. The formulation is configured as an anti-parasitic, anti-inflammatory and anti-microbial formulation.

The invention discloses a method for identifying native tea tree varieties by utilizing characteristic metabolites, and particularly relates to the field of tea tree variety identification, which comprises the steps of sample preparation, metabolite extraction, liquid phase mass spectrometry detection, data correction processing, characteristic metabolite construction, multivariate statistical clustering judgment and the like. The method comprises the following steps: constructing a high-dimensional characteristic metabolite vector by utilizing a stable numerical value distribution mode of fresh leaves on sixteen characteristic metabolites according to internal metabolic characteristics formed in a long-term natural domestication process of the castanopsis sclerophylla leaf group species; after peak intensity data of each sample is corrected, filtered and structured, obtained feature vectors are input into an Euclidean distance hierarchical clustering model, and unsupervised statistical judgment on the metabolic composition difference between the to-be-identified sample and the standard floating beam leaf group species is realized.

The present invention relates to the cosmetic field. In particular, the present invention relates to a particular oily extract of a Melaleuca alternifolia, to a cosmetic composition comprising the same, and to the use thereof as a cosmetic regeneration, repairing or anti-fatigue agent for healthy skin.

Provided are an agent for increasing polyphenol content and an agent for increasing theanine content in leaves of a plant of the family Theaceae, these agents making it possible to safely increase the amount of polyphenols, such as catechins, and / or theanine contained in the tea tree by appropriately spraying or irrigating the plant without adversely affecting the biological tissue of the plant. The agent for increasing the polyphenol content and / or the theanine content in leaves of a plant of the family Theaceae contains at least one compound selected from the group consisting of oxo fatty acids or salts thereof and fatty acid hydroxides or salts thereof.

The application discloses application of a LtCel61A0 (Lasiodiplodia theobromae glycoside hydrolase family 61) gene in regulating growth, reproduction, pathogenicity and adversity resistance of Lasiodiplodia theobromae. The LtCel61A0 gene is derived from the L. theobromae, a knockdown or overexpression vector of the LtCel61A0 gene of the L. theobromae is constructed, and the vector is introduced into protoplast of the L. theobromae to obtain a knockdown or overexpression strain. It is found through experiments that the LtCel61A0 gene is significantly affected by the knockdown or overexpression, has an effect on the adversity resistance of the L. theobromae, and significantly reduces the pathogenicity of the L. theobromae to tea tree leaves. Therefore, the LtCel61A0 can be used as a fungicide target and a key protein of a pathogenic mechanism of a disease, is used for developing a medicament of a disease caused by the L. theobromae and cultivating a new variety of a disease-resistant plant, and has a wide application prospect in the prevention and treatment of plant pathogenic fungal diseases.

The present invention discloses an endophytic fungus of tea tree and its application in promoting the growth of Dendrobium officinale, belonging to the field of microbial technology. The abnormal Wickerham yeast strain (Wickerhamomyces p.) involved in the present invention was deposited in the General Microbiological Center of China Microbiological Culture Collection Administration on September 15, 2022. The strain preservation name is: KLBMP0506, and the preservation number is: CGMCC No. 25719. It has a significant effect on promoting the growth of Dendrobium officinale and the accumulation of secondary metabolites, provides excellent strain resources for the development of rare medicinal Dendrobium plant growth-promoting fertilizers, promotes the industrialization development of Dendrobium, and has good application development prospects.

The application discloses application of cembrene in inhibition of a disease development process of a tea tree annular spot, and belongs to the technical field of tea tree annular spot prevention and treatment. In vitro, standard cembrene and Pestalotiopsis funerea mycelium blocks are co-cultured, the MIC50 value is calculated to measure the bacteriostatic effect, and when the cembrene is co-cultured with a colletotrichum genus fungus, it is also found that the cembrene has a good inhibitory effect on mycelium growth. Meanwhile, it is also found that the cembrene can damage Pestalotiopsis funerea spore cells and affect the pathogenicity of the Pestalotiopsis funerea to tea tree leaves. Compared with traditional bacteriostatic agents, the application provides a more effective and environment-friendly bacteriostatic agent for inhibiting plant annular spot in the market.