6 results about "Melaleuca alternifolia" patented technology

The application discloses application of a LtCel61A0 (Lasiodiplodia theobromae glycoside hydrolase family 61) gene in regulating growth, reproduction, pathogenicity and adversity resistance of Lasiodiplodia theobromae. The LtCel61A0 gene is derived from the L. theobromae, a knockdown or overexpression vector of the LtCel61A0 gene of the L. theobromae is constructed, and the vector is introduced into protoplast of the L. theobromae to obtain a knockdown or overexpression strain. It is found through experiments that the LtCel61A0 gene is significantly affected by the knockdown or overexpression, has an effect on the adversity resistance of the L. theobromae, and significantly reduces the pathogenicity of the L. theobromae to tea tree leaves. Therefore, the LtCel61A0 can be used as a fungicide target and a key protein of a pathogenic mechanism of a disease, is used for developing a medicament of a disease caused by the L. theobromae and cultivating a new variety of a disease-resistant plant, and has a wide application prospect in the prevention and treatment of plant pathogenic fungal diseases.

The application discloses application of a LtEgID9 (Lasiodiplodia theobromae glycoside hydrolase family 61) gene in regulation of pathogenicity and stress resistance of Lasiodiplodia theobromae. The LtEgID9 gene is derived from L. theobromae, a knockdown vector of the LtEgID9 gene of L. theobromae is constructed, and the vector is introduced into protoplasts of L. theobromae to obtain a knockdown strain. It is found through experiments that the knockdown of the LtEgID9 gene can have an effect on the stress resistance of L. theobromae without affecting the growth of L. theobromae, and significantly reduce the pathogenicity of L. theobromae to tea tree leaves. Therefore, the LtEgID9 can be used as a fungicide target and a key protein of a pathogenic mechanism of a disease, for development of a medicament for the disease caused by L. theobromae infection and cultivation of a new variety of disease-resistant plants, and has a wide application prospect in the prevention and treatment of plant pathogenic fungal diseases.

This invention discloses a tissue culture-free regeneration method for tea trees based on leaf cuttings, comprising the following steps: First, select mature, healthy leaves of a tea variety, retaining the complete petiole; then, treat the base of the petiole with a first exogenous hormone solution, and then insert the leaf cuttings into a substrate; cultivate under controlled light, temperature, and humidity conditions, and spray the leaves with a second exogenous hormone solution to induce the growth of callus tissue, adventitious roots, and adventitious buds. This invention employs a tissue culture-free method, through specific hormone treatment and environmental regulation, to induce the formation of callus tissue, adventitious roots, and adventitious buds at the base of the petiole of tea leaves, ultimately forming a complete plant. This significantly improves the regeneration efficiency of tea leaf cuttings, simultaneously achieving root and bud formation to form a complete plant, and has broad application prospects and significant economic value.

The utility model belongs to the technical field of seedling transplanting, and particularly relates to an automatic transplanting device for melaleuca alternifolia tissue culture seedlings, which comprises a main plate body, supporting rods are fixedly connected to four corners of the bottom of the main plate body, a seedling box is fixedly connected to the top surface of the main plate body, and universal wheels are fixedly connected to the bottoms of the supporting rods. A vertically downward hydraulic rod is fixedly connected to the center of the bottom of the main plate body, a lifting plate is fixedly connected to the bottom end of the hydraulic rod, two sliding grooves which are symmetrical left and right are formed in the bottom of the lifting plate, sliding blocks which are symmetrical to each other are slidably inserted into the two sliding grooves, and the interior of the lifting plate is a cavity; a cavity is formed in the upper portion of the base, two sliding blocks are arranged in the cavity, a driving structure enabling the two sliding blocks to move oppositely is arranged in the cavity, the bottoms of the two sliding blocks are fixedly connected with connecting plates, the bottoms of the two connecting plates are fixedly connected with soil turning plates, and the two soil turning plates can be combined together. After transplanting is completed, the turned soil is automatically reset, and the transplanting efficiency is improved.

The application provides a long-acting compound essential oil and a preparation method thereof, and belongs to the technical field of compound essential oil.The compound essential oil composition comprises sweet orange essential oil, bergamot essential oil, lavender essential oil, ylang-ylang essential oil, melaleuca alternifolia essential oil, patchouli essential oil, cedar essential oil, vetiver essential oil and sandalwood essential oil.The application effectively solves the technical problems of short fragrance retention time, easy and rapid loss of fragrance, and unstable fragrance level of the existing essential oil composition by scientifically proportioning essential oil components with different volatile rates, achieves long-acting, stable and level fragrance retention effect, and can effectively relieve human anxiety, calm the nerves and relax the body and mind.

The present application belongs to the technical field of economic crop biological prevention and control, and provides a plant source attractant for Empoasca vitis and application thereof. The plant source attractant for Empoasca vitis is composed of farnesene, linalool and leaf alcohol. The attractant is placed at tea shoots in early spring and early autumn when adult insects occur, and can be prepared into a lure core and other preparation forms. In order to achieve better trapping effect, the attractant can be used in combination with a yellow yellow fever mosquito plate. The attractant is of plant source and is safe to the environment, has the characteristics of high efficiency, greenness and convenience, and has a wide industrialization prospect.