Agricultural composition for preventing and controlling strawberry gray mold

Abstract

The present invention discloses an agricultural composition for controlling strawberry gray mold, belonging to the technical field of agricultural compositions. The agricultural composition provided by the present invention comprises resveratrol and a Melaleuca alternifolia extract as active ingredients, with the mass ratio of resveratrol to the Melaleuca alternifolia extract being 1:20 to 5:1. The present invention combines resveratrol and the Melaleuca alternifolia extract for controlling strawberry gray mold, exhibiting a synergistic effect.

Description

Technical Field

[0001] The present invention relates to the technical field of pesticides, in particular to an agricultural composition for preventing and treating strawberry gray mold. Background Art

[0002] Gray mold is a significant disease affecting strawberry cultivation and growth. It is particularly susceptible to cold and high humidity conditions, particularly in greenhouse strawberries. It primarily harms the flowers and fruit, but also leaves, petioles, and pedicels. Gray mold typically reduces strawberry yields by 20%-30%, and in severe cases, by over 50%, or even completely destroys the entire crop.

[0003] Existing technologies for controlling strawberry gray mold primarily include disease-resistant varieties, crop rotation, field management, and chemical control, with chemical control being the primary approach. Due to the long-term use of chemical pesticides and their inherent shortcomings, strawberry gray mold has developed varying degrees of resistance to various agents, resulting in reduced control effectiveness, increased chemical pesticide usage, increased risk of pesticide residues, and greater environmental pollution. However, biopesticides are naturally derived, easily degradable, have low residues, and are safe and environmentally friendly. Therefore, the development of highly effective, low-toxic, and low-pollution biopesticides is of great significance.

[0004] Resveratrol and Melaleuca alternifolia extract are botanical pesticides, classified as biopesticides. Through extensive testing, the applicant has discovered that a combination of resveratrol and Melaleuca alternifolia extract has a synergistic effect on inhibiting gray mold in strawberries. Currently, there are no reports on the combination of resveratrol and Melaleuca alternifolia extract. Summary of the Invention

[0005] To solve the above technical problems, the present invention aims to provide an agricultural composition for preventing and controlling gray mold of strawberry, so as to improve the prevention and control effect of gray mold of strawberry and reduce pesticide residues.

[0006] To achieve the technical purpose of the present invention, on the one hand, the present invention relates to an agricultural composition for preventing and controlling strawberry gray mold, the active ingredients of which are composed of resveratrol and Melaleuca alternifolia extract, and the mass ratio of resveratrol to Melaleuca alternifolia extract is 1:20 to 5:1.

[0007] Furthermore, in the agricultural composition for preventing and controlling strawberry gray mold provided by the present invention, the mass ratio of resveratrol to the Melaleuca alternifolia extract is 1:10 to 1:5.

[0008] Furthermore, in the agricultural composition for preventing and controlling strawberry gray mold provided by the present invention, the pesticide registration number of resveratrol is PD20212931, and the pesticide registration number of Melaleuca alternifolia extract is PD20190103.

[0009] Depending on actual application needs, the agricultural compositions provided herein can be prepared into various dosage forms using methods known to those skilled in the art. The agricultural compositions provided herein may also contain adjuvants required for preparing pesticide formulations. These adjuvants may be solvents, emulsifiers, wetting agents, stabilizers, dispersants, thickeners, pH adjusters, defoamers, antifreeze agents, fillers, or a combination thereof. The adjuvants described herein can all be known substances, such as various adjuvants commonly used in pesticide formulations, and may vary depending on the application.

[0010] On the other hand, the present invention relates to the use of the above-mentioned agricultural composition for preventing and controlling strawberry gray mold in preventing and controlling agricultural diseases.

[0011] Furthermore, in the application provided by the present invention, the agricultural disease is strawberry gray mold.

[0012] Compared with the prior art, the technical solution provided by the present invention has at least the following beneficial effects:

[0013] The agricultural composition provided by the present invention combines resveratrol with a Melaleuca alternifolia extract, demonstrating a synergistic effect against strawberry gray mold in a mass ratio of 1:20 to 5:1. The agricultural composition selected by the present invention is easily degradable in nature, reducing pesticide residues in agricultural products and ensuring their safety. DETAILED DESCRIPTION

[0014] The technical solutions of the present invention are described below with reference to the following examples. However, the present invention is not limited to the following examples. The experimental methods and detection methods described in each example are conventional methods unless otherwise specified; the reagents and materials described are commercially available unless otherwise specified.

[0015] Test agent:

[0016] Resveratrol was purchased from Inner Mongolia Qingyuanbao Biotechnology Co., Ltd., registration number: PD20212931;

[0017] Melaleuca alternifolia extract was purchased from Stockton (Israel) Ltd., registration number: PD20190103.

[0018] Example: Indoor bioassay of resveratrol and Melaleuca alternifolia extract against strawberry gray mold

[0019] Test crop: Strawberry, variety: Hongyan.

[0020] Test strains: Strawberry plants showing gray mold were collected from a strawberry greenhouse in Huyi District, Xi'an City, Shaanxi Province. The plants were isolated and purified in the laboratory. The pathogen was identified as gray mold and stored on PDA culture medium.

[0021] Test agents: The test agents were first dissolved in acetone and then diluted with 0.1% Tween-80 aqueous solution to prepare single-dose stock solutions and mixed stock solutions of various proportions. Five mass concentration gradients were set for each single agent and mixed solution according to the equal proportion method.

[0022] Test method: The mycelium growth rate method was used for determination. Use a puncher to cut the pathogen cake cultured on the PDA medium, and inoculate the mycelium side down in the center of the solidified drug-containing PDA medium. Set up a blank control without drugs, and repeat 3 times for each treatment. Culture under the conditions of temperature 23°C, relative humidity 90%, and 12h alternating light and dark (light intensity 5000Lux). When the diameter of the control colony grows to be larger than 2 / 3 of the diameter of the plate, use a caliper to measure the diameter of each treated colony. Calculate the mycelium growth inhibition rate of each treatment. With the logarithm of the drug concentration as the horizontal axis and the probability value of the mycelium growth inhibition rate as the vertical axis, the regression analysis method was used to analyze the experimental data and calculate the EC of each treatment. 50 The test results are shown in Table 1.

[0023] Growth inhibition rate (%) = (control colony diameter - treated colony diameter) × 100 / (control colony diameter - cake diameter)

[0024] Synergistic evaluation method: Wadley method was used for evaluation, where SR = 0.5-1.5, the combination of the two agents had an additive effect; SR ≤ 0.5, it was an antagonistic effect; SR ≥ 1.5, it was a synergistic effect.

[0025] EC 50 (Theoretical value) = (a + b) / (a ​​ / EC 50 a+b / EC 50 b);

[0026] SR=EC 50 (Theoretical value) / EC 50 (actual value);

[0027] Where SR is the synergistic coefficient, and a and b are the percentages of the components in the mixture.

[0028] Table 1 Indoor bioassay results of the compound against strawberry gray mold

[0029]

[0030] As shown in Table 1, resveratrol and Melaleuca alternifolia extracts used alone have a good inhibitory effect on strawberry gray mold. 50The results are 5.24g / L and 19.07g / L, respectively. The combination of resveratrol and Melaleuca alternifolia extract can enhance the inhibitory effect of the agent on strawberry gray mold. When the mass ratio of resveratrol to Melaleuca alternifolia extract is 1:20 to 5:1, the combination has a significant inhibitory effect on strawberry gray mold, showing a synergistic effect. In particular, when the mass ratio of resveratrol to Melaleuca alternifolia extract is 1:10 to 1:5, the synergistic coefficient reaches above 2.5, indicating a significant synergistic effect.

[0031] The embodiments described above are some of the embodiments of the present invention, rather than all of them. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but rather merely represents selected embodiments of the present invention. All other embodiments obtained without creative effort and through deduction and substitution by a person of ordinary skill in the art based on the concept of the present invention are within the scope of protection of the present invention.

Claims

1. An agricultural composition for preventing and treating strawberry gray mold, characterized in that: The active ingredients are composed of resveratrol and Melaleuca alternifolia extract, and the mass ratio of resveratrol to Melaleuca alternifolia extract is 1:20 to 5:1; The pesticide registration number of the resveratrol in the composition is PD20212931, and the pesticide registration number of the Melaleuca alternifolia extract in the composition is PD20190103.

2. The agricultural composition for preventing and treating strawberry gray mold according to claim 1, wherein The mass ratio of resveratrol to the Melaleuca alternifolia extract in the composition is 1:10 to 1:

5.

3. Use of the agricultural composition for preventing and controlling strawberry gray mold according to any one of claims 1 to 2 in preventing and controlling agricultural diseases.

4. The use according to claim 3, characterized in that The agricultural disease is strawberry gray mold.

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