The invention relates to the field of dressings, in particular to a preparation method and application of an acellular biological matrix dressing. The decellularized biological matrix dressing is prepared by the following steps: decellularizing mammalian tissues, sequentially carrying out
ion induction and crushing to obtain a crushed product, carrying out
surface finishing and
vacuum drying on the crushed product, and mixing to obtain the decellularized biological matrix dressing. The obtained acellular biological matrix dressing has a rougher surface and a more uniform roughness degree, so that the stability of the final product acellular biological matrix dressing and the capability of promoting
cell adhesion are improved. When in use, the particle can be directly applied to various wounds, the complexity of using the particle in the prior art is reduced, and the particle can be directly covered on full-thickness
skin defect wounds (such as deep wet wounds) and
superficial wounds (such as dry epidermis injury).