A dressing containing polysaccharide and its preparation method and application

Abstract

The present invention belongs to the technical field of biomedical dressings, and discloses a dressing containing polysaccharides, and a preparation method and application thereof. The polysaccharide-containing dressing comprises ganoderma lucidum β-glucan, ganoderma lucidum chitosan, glycerol and carbomer. The present invention uses ganoderma lucidum β-glucan and ganoderma lucidum chitosan, which are key active ingredients of ganoderma lucidum, as main raw materials, and glycerol and carbomer as auxiliary raw materials to prepare a dressing containing polysaccharides, which can be applied to the covering and care of non-chronic wounds, such as superficial wounds, first-degree or shallow second-degree burn wounds, surgical suture wounds, laser / photon / acid peeling / microneedle treatment / micro-plastic surgery wounds, to provide a microenvironment for wound healing, and promote the repair and healing of wounds. The dressing has simple ingredients and high safety, and can be widely used in the preparation of scald treatment drugs or non-chronic wound care products.

Description

Technical Field

[0001] The invention belongs to the technical field of biomedical dressings, and in particular relates to a dressing containing polysaccharides, a preparation method and an application thereof. Background Art

[0002] With the development of medical technology, gel dressings are increasingly used, and they have many advantages over traditional gauze dressings. As a commonly used wound dressing, gel dressings can absorb the exudate from the patient's wound and keep the wound moist and clean, thereby providing a good healing environment for the wound. They are suitable for superficial and non-chronic wounds.

[0003] For non-chronic wounds on certain facial or exposed areas, such as superficial wounds, first-degree or shallow second-degree burn wounds, surgical suture wounds, and wounds formed after laser / photon / acid peeling / microneedle treatment of micro plastic surgery, patients or users have more stringent requirements for the repair time and repair effect of the dressing, and the dressing should not adhere to the wound when removed, causing secondary damage to the wound. In addition, users also have high expectations for the safety of the dressing ingredients and the user experience.

[0004] At present, the gel dressings on the market are mainly sodium polyacrylate gel dressings and carrageenan gel dressings, which mainly play the role of absorbing fluid and replenishing water. However, these dressings often do not have the ability to quickly heal wounds, and dressings with added active pharmaceutical ingredients often have complex ingredients, and users have more concerns about safety.

[0005] Therefore, there is an urgent need to provide a dressing for non-chronic wounds that can quickly heal and repair the wound and has simple ingredients and is safe and reliable. Summary of the invention

[0006] The present invention aims to solve at least one of the technical problems existing in the above-mentioned prior art. To this end, the present invention provides a dressing containing polysaccharide and a preparation method and application thereof. The dressing provided by the present invention has simple ingredients and can provide a microenvironment for wound healing, so as to quickly heal and repair the wound.

[0007] The invention provides a dressing containing polysaccharide.

[0008] Specifically, a polysaccharide-containing dressing includes ganoderma lucidum beta-glucan, ganoderma lucidum chitosan, glycerol and carbomer.

[0009] Preferably, the dressing further comprises water. In parts by mass, the dressing comprises 1-5 parts of Ganoderma lucidum β-glucan, 5-15 parts of Ganoderma lucidum chitosan, 1-5 parts of glycerol, 0.1-0.5 parts of carbomer and 70-90 parts of water.

[0010] Further preferably, the dressing comprises, by weight, 2-5 parts of Ganoderma lucidum β-glucan, 5-10 parts of Ganoderma lucidum chitosan, 2-5 parts of glycerol, 0.1-0.3 parts of carbomer and 75-90 parts of water.

[0011] Preferably, the mass ratio of the Ganoderma lucidum β-glucan to the Ganoderma lucidum chitosan and the glycerol is (0.3-0.8):1:(0.2-0.8); further preferably, the mass ratio of the Ganoderma lucidum β-glucan to the Ganoderma lucidum chitosan and the glycerol is (0.4-0.6):1:(0.32-0.6); more preferably, the mass ratio of the Ganoderma lucidum β-glucan to the Ganoderma lucidum chitosan and the glycerol is (0.4-0.6):1:(0.45-0.6).

[0012] Preferably, the mass ratio of the carbomer to the ganoderma lucidum chitosan is (0.025-0.1):1; further preferably, the mass ratio of the carbomer to the ganoderma lucidum chitosan is (0.03-0.06):1.

[0013] Preferably, the dressing further comprises a pH adjusting agent.

[0014] Preferably, the pH adjuster is selected from at least one of citric acid, citric acid, malic acid, chlorogenic acid, palmitic acid, glacial acetic acid, triethanolamine, sodium hydroxide and potassium hydroxide; further preferably, the pH adjuster is triethanolamine. Selecting triethanolamine as the pH adjuster can reduce the effect of the pH adjuster on the structure of Ganoderma lucidum β-glucan and Ganoderma lucidum chitosan, and improve the dressing stability and wound repair ability.

[0015] Preferably, the dressing further comprises a preservative. The preservative is selected from one of benzyl alcohol, ethanol and methyl paraben. Further preferably, the preservative is methyl paraben, which can effectively inhibit microorganisms such as bacteria, molds, yeasts, and play a role in inhibiting bacterial growth; and can also protect active ingredients such as Ganoderma lucidum β-glucan and Ganoderma lucidum chitosan.

[0016] The invention also provides a method for preparing the polysaccharide-containing dressing.

[0017] Specifically, a method for preparing a dressing containing polysaccharides comprises the following steps:

[0018] The components are mixed to prepare a dressing containing polysaccharide.

[0019] Preferably, a method for preparing a polysaccharide-containing dressing comprises the following steps:

[0020] Ganoderma lucidum beta-glucan and carbomer are added into water and mixed, and then Ganoderma lucidum chitosan and glycerol are added and ultrasonically mixed to prepare a dressing containing polysaccharides.

[0021] When the dressing further contains a pH regulator and a preservative, the preparation method of the dressing containing polysaccharides comprises the following steps:

[0022] Ganoderma lucidum beta-glucan, carbomer and pH regulator are added into water and mixed, and then Ganoderma lucidum chitosan, glycerol and preservative are added and ultrasonically mixed to prepare a dressing containing polysaccharide.

[0023] The invention also provides an application of a dressing containing polysaccharide.

[0024] Specifically, the polysaccharide-containing dressing is used in the preparation of medicines for treating burns.

[0025] Specifically, the polysaccharide-containing dressing is used in the preparation of non-chronic wound care products.

[0026] The invention also provides a scald medicine.

[0027] Specifically, a burn medicine comprises the above-mentioned polysaccharide-containing dressing.

[0028] The invention also provides a non-chronic wound care product.

[0029] Specifically, a non-chronic wound care product comprises the above-mentioned polysaccharide-containing dressing and a medical non-woven fabric carrying the dressing.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] (1) The present invention uses the key active ingredients of Ganoderma lucidum, Ganoderma lucidum β-glucan and Ganoderma lucidum chitosan, as the main raw materials, and glycerol and carbomer as auxiliary raw materials to prepare a dressing containing polysaccharides. The dressing can be used to cover and care for non-chronic wounds (such as superficial wounds, first-degree or shallow second-degree burn wounds, surgical suture wounds, laser / photon / fruit acid peeling / microneedle treatment / micro-plastic surgery wounds), provide a microenvironment for wound healing, and promote the repair and healing of wounds. Among them, Ganoderma lucidum β-glucan contains anti-inflammatory cytokines, which plays an essential role in reversing the inflammatory process; Ganoderma lucidum chitosan has the effects of antibacterial, hemostatic, analgesic, promoting wound healing and reducing scar formation; glycerol can retain moisture for dry and scabby wounds, promote autolytic debridement and exogenous debridement, keep the wound in an appropriate moist state, and effectively avoid dry necrosis of wound tissue; carbomer has the function of adjusting the viscosity of the product and forming a film on the skin surface to absorb tissue exudate. The combined use of the four can effectively prevent wound inflammation, improve tissue regeneration ability, and accelerate wound healing.

[0032] (2) The polysaccharide-containing dressing provided by the present invention has simple ingredients and high safety, and can be widely used in the preparation of scald treatment drugs or non-chronic wound care products. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 This is a diagram of the wound healing effect in a mouse wound healing experiment;

[0034] Figure 2 This is a picture of the wound surface in the rat deep second-degree burn healing experiment. DETAILED DESCRIPTION

[0035] In order to make the technical scheme of the present invention more clearly understood by those skilled in the art, the following embodiments are listed for illustration. It should be pointed out that the following embodiments do not limit the protection scope of the present invention.

[0036] The Ganoderma lucidum β-glucan and Ganoderma lucidum chitosan used in the following examples and comparative examples were purchased from Draco Natural Products, Inc. The other raw materials, reagents or devices used, unless otherwise specified, can be obtained from conventional commercial channels or can be obtained by existing known methods.

[0037] Example 1

[0038] A polysaccharide-containing dressing comprises, by weight, 3.5 parts of ganoderma lucidum beta-glucan, 6 parts of ganoderma lucidum chitosan, 3 parts of glycerol, 0.25 parts of carbomer, 0.15 parts of triethanolamine, 0.3 parts of methylparaben and 86.8 parts of deionized water.

[0039] A method for preparing a dressing containing polysaccharides comprises the following steps: adding ganoderma lucidum beta-glucan, carbomer and triethanolamine into deionized water, preliminarily mixing, then adding ganoderma lucidum chitosan, glycerol and methyl paraben, and mixing evenly in an ultrasonic oscillator to prepare a dressing containing polysaccharides.

[0040] Example 2

[0041] A polysaccharide-containing dressing comprises, by weight, 3 parts of ganoderma lucidum beta-glucan, 6 parts of ganoderma lucidum chitosan, 3.6 parts of glycerol, 0.3 parts of carbomer, 0.15 parts of triethanolamine, 0.3 parts of methylparaben and 86.65 parts of deionized water.

[0042] A method for preparing a dressing containing polysaccharides comprises the following steps: adding ganoderma lucidum beta-glucan, carbomer and triethanolamine into deionized water, preliminarily mixing, then adding ganoderma lucidum chitosan, glycerol and methyl paraben, and mixing evenly in an ultrasonic oscillator to prepare a dressing containing polysaccharides.

[0043] Example 3

[0044] A polysaccharide-containing dressing comprises, by weight, 2 parts of ganoderma lucidum beta-glucan, 8 parts of ganoderma lucidum chitosan, 3 parts of glycerol, 0.25 parts of carbomer, 0.15 parts of triethanolamine, 0.3 parts of methylparaben and 86.3 parts of deionized water.

[0045] A method for preparing a dressing containing polysaccharides comprises the following steps: adding ganoderma lucidum beta-glucan, carbomer and triethanolamine into deionized water, preliminarily mixing, then adding ganoderma lucidum chitosan, glycerol and methyl paraben, and mixing evenly in an ultrasonic oscillator to prepare a dressing containing polysaccharides.

[0046] Example 4

[0047] A polysaccharide-containing dressing comprises, by weight, 3.5 parts of ganoderma lucidum beta-glucan, 6 parts of ganoderma lucidum chitosan, 3 parts of glycerol, 0.25 parts of carbomer, 0.15 parts of triethanolamine, 0.3 parts of benzyl alcohol and 86.8 parts of deionized water.

[0048] A method for preparing a dressing containing polysaccharides comprises the following steps: adding ganoderma lucidum beta-glucan, carbomer and triethanolamine into deionized water, preliminarily mixing, then adding ganoderma lucidum chitosan, glycerol and benzyl alcohol, mixing evenly in an ultrasonic oscillator, and preparing the dressing containing polysaccharides.

[0049] Comparative Example 1

[0050] A polysaccharide-containing dressing comprises, by weight, 3.5 parts of oat beta-glucan, 6 parts of ganoderma lucidum chitosan, 3 parts of glycerol, 0.25 parts of carbomer, 0.15 parts of triethanolamine, 0.3 parts of methylparaben and 86.8 parts of deionized water.

[0051] A method for preparing a polysaccharide-containing dressing comprises the following steps: adding oat beta-glucan, carbomer and triethanolamine into deionized water, preliminarily mixing, then adding ganoderma lucidum chitosan, glycerol and methyl paraben, and mixing evenly in an ultrasonic oscillator to prepare the polysaccharide-containing dressing.

[0052] Comparative Example 2

[0053] A polysaccharide-containing dressing comprises, by weight, 3.5 parts of yeast beta-glucan, 6 parts of ganoderma lucidum chitosan, 3 parts of glycerol, 0.25 parts of carbomer, 0.15 parts of triethanolamine, 0.3 parts of methylparaben and 86.8 parts of deionized water.

[0054] A method for preparing a polysaccharide-containing dressing comprises the following steps: adding yeast beta-glucan, carbomer and triethanolamine into deionized water, preliminarily mixing, then adding ganoderma lucidum chitosan, glycerol and methyl paraben, and mixing evenly in an ultrasonic oscillator to prepare the polysaccharide-containing dressing.

[0055] Comparative Example 3

[0056] A polysaccharide-containing dressing comprises, by weight, 3.5 parts of ganoderma lucidum beta-glucan, 6 parts of ganoderma lucidum chitosan, 3 parts of glycerol, 0.25 parts of carrageenan, 0.15 parts of triethanolamine, 0.3 parts of methylparaben and 86.8 parts of deionized water.

[0057] A method for preparing a polysaccharide-containing dressing comprises the following steps: adding ganoderma lucidum beta-glucan, carrageenan and triethanolamine into deionized water, preliminarily mixing, then adding ganoderma lucidum chitosan, glycerol and methyl paraben, and mixing evenly in an ultrasonic oscillator to prepare the polysaccharide-containing dressing.

[0058] Application Example 1

[0059] A non-chronic wound care product comprises a polysaccharide-containing dressing prepared in Example 1 and a medical non-woven fabric carrying the dressing. The specific method is as follows: the medical non-woven fabric is soaked in the dressing prepared in Example 1, taken out after ultrasonic treatment for a period of time, and freeze-dried to obtain the non-chronic wound care product.

[0060] Product effect testing

[0061] 1. Mouse wound healing experiment

[0062] By observing the local skin healing, the potential healing of the test product on the contact tissue was evaluated. Methods: 48 female SPF mice. After the quarantine, the skin was excised on the back of the anesthetized mouse under sterile conditions to create a 2.0cm*1.0cm full-thickness skin defect wound. The depth was based on reaching the fascia, and the damaged tissue was seen to leak tissue fluid. The skin wound model was induced, the wound was photographed, and the wound area was recorded, which was the area d0 at this time. The model mice were randomly divided into a model control group, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Comparative Example 1 group, Comparative Example 2 group and Comparative Example 3 group, 6 mice / group. The wounds of each group of mice were smeared with the corresponding test samples, and the mice in the model control group were treated with an equal amount of normal saline, once a day, for 15 consecutive days. The wounds of the mice were photographed (with a ruler) and measured on the 3rd, 6th, 9th, 12th and 15th days, and the wound areas were measured using software. The wound areas were recorded as area d3, area d6, area d9, area d12 and area d15, respectively.

[0063] The test samples were applied daily, and on the 0th, 3rd, 6th, 9th, 12th and 15th days after surgery, the wound color changes, exudate, granulation tissue, growth and epithelial growth were observed to evaluate the wound healing effect and calculate the wound contraction rate (%).

[0064] Wound healing rate (%) = (area d0-area dn) / area d0×100%;

[0065] Among them, area dn is area d3, area d6, area d9, area d12 and area d15.

[0066] All data were expressed as (x±s), and SPSS21.0 software was used for statistical analysis; one-way ANOVA was used for statistical analysis. The test results are shown in Table 1.

[0067] Table 1 Wound healing rate (%)

[0068]

[0069]

[0070] As shown in Table 1, the dressings provided in the examples and comparative examples all have the effect of promoting the healing of early wounds, and the healing effect of Example 1 is the most obvious (the wound healing effect diagram is shown in FIG. Figure 1 As shown), the wound healing ability of the dressing provided by Examples 1-4 is significantly better than that of Comparative Examples 1-3. The total amount of Ganoderma lucidum β-glucan and Ganoderma lucidum chitosan in Example 3 is comparable to that in Example 1, but due to different ratios, its effect is slightly worse than that of Example 1. After methyl paraben is replaced by benzyl alcohol in Example 4, its effect is also slightly worse. Although benzyl alcohol and methyl paraben are both preservatives, the inventors found that methyl paraben can not only play an antibacterial and antiseptic role, but also protect Ganoderma lucidum β-glucan and Ganoderma lucidum chitosan, which is conducive to improving the mid-term wound healing rate. After Ganoderma lucidum β-glucan is replaced by oat β-glucan and yeast β-glucan in Comparative Examples 1 and 2, although both have a certain ability to promote wound healing, their effect is obviously not as good as that of Example 1. This may be related to the special chain conformation or branching degree of Ganoderma lucidum β-glucan, and Ganoderma lucidum chitosan and Ganoderma lucidum β-glucan can work together to significantly promote the wound healing effect. Comparative Example 3 replaces carbomer with carrageenan, and its effect is obviously not as good as that of Example 1, especially in the early stage, it cannot promote wound healing well. Although carrageenan and carbomer can both be used as thickeners, and the sulfated polysaccharide structure in carrageenan can physically block viruses with sugar molecules on the surface and have an inhibitory effect on viruses, the coordination effect of carrageenan with Ganoderma lucidum β-glucan and Ganoderma lucidum chitosan is not as good as that of carbomer. Carbomer has good affinity with the skin, forms a film on the skin surface to absorb tissue exudate, and is used in combination with Ganoderma lucidum β-glucan and Ganoderma lucidum chitosan to promote the healing effect of Ganoderma lucidum β-glucan and Ganoderma lucidum chitosan on the skin, reduce the irritation of the dressing to the skin, and improve the user's experience.

[0071] 2. Experimental study on healing of deep second degree burns in rats

[0072] The polysaccharide-containing dressing prepared in Example 1 was applied to the deep II degree burn healing experiment in rats. The deep II degree burn model of rats was prepared and treated in groups: rats were used as experimental animals to make a rat burn model, and the polysaccharide-containing dressing prepared in Example 1 was evaluated for its healing effect on burn wounds. Twelve experimental rats, half male and half female, weighing 200g±10, were divided into a control group and an experimental group, with 6 rats in each group. The back of the rats was depilated with a depilatory cream, wiped clean with saline, and anesthetized with 3% sodium pentobarbital injected intraperitoneally. The deep II degree burn ulcer model of rats was prepared in the same way. At the end of the burn, the surface cortex of the burn was scraped off with a sterile blade, and the wound was wiped clean with saline. The burn wounds of the control group rats were not treated, and the experimental group was attached to the burn wounds of the rats with the non-chronic wound care product prepared in Example 1, and the wound healing was observed every day, and the non-chronic wound care product was replaced every 2d.

[0073] Healing of deep second-degree burn ulcer wounds in rats: 1 day after the burn, the wound surface of the control group was red and there was tissue fluid exudation; the dressing of the experimental group adhered to the wound surface, and there was no obvious tissue fluid exudation. Carefully open the non-chronic wound care product, the care product is easy to fall off and not easy to adhere to the wound surface, and the wound surface is only slightly red. The wound surface conditions of the rats in the control group and the experimental group are as follows Figure 2 As shown. 5 days after the scald, the control group formed a scab, but the scab was not complete; the experimental group had complete scabs, and the peripheral scabs began to peel off. 10 days after the scald, the scabs on the wound surface of the control group were hard and dark red in color, and the periphery of the scabs dissolved; most of the scabs in the experimental group fell off, forming new skin, and the wound surface began to heal. The experimental results show that the polysaccharide-containing dressing prepared in Example 1 of the present invention can significantly shorten the healing time of deep II degree burn wounds in rats.

[0074] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A dressing containing polysaccharides, characterized in that: The composition comprises, by weight, 3.5 parts of ganoderma lucidum beta-glucan, 6 parts of ganoderma lucidum chitosan, 3 parts of glycerol, 0.25 parts of carbomer, 0.15 parts of triethanolamine, 0.3 parts of methylparaben and 86.8 parts of deionized water.

2. The method for preparing the polysaccharide-containing dressing according to claim 1, characterized in that: The following steps are involved: The components are mixed to prepare a dressing containing polysaccharide.

3. The polysaccharide-containing dressing according to claim 1 is used in the preparation of scald treatment medicines or non-chronic wound care products.

4. A scald medicine, characterized in that: A dressing comprising the polysaccharide according to claim 1.

5. A non-chronic wound care product, characterized in that: The invention comprises the polysaccharide-containing dressing according to claim 1 and a medical nonwoven fabric carrying the dressing.

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