Anti-pollution acne-sensitive skin repairing composition containing golden camellia flower extract and application of anti-pollution acne-sensitive skin repairing composition

Abstract

The invention discloses a camellia nitidissima flower extract-containing anti-pollution acne-sensitive skin repairing composition and application thereof, and relates to the technical field of cosmetics. The invention provides a camellia nitidissima flower extract-containing anti-pollution acne-sensitive skin repairing composition. The camellia nitidissima flower extract-containing anti-pollution acne-sensitive skin repairing composition is prepared from the following components in parts by weight: 1 to 5 parts of camellia nitidissima flower extract, 1 to 5 parts of seaweed oligosaccharide, 0.01 to 1 part of o-cymene-5-ol and 0.01 to 1 part of tocopherol retinoate. According to the anti-pollution acne-sensitive skin repairing composition containing the camellia nitidissima flower extract, four components including the camellia nitidissima flower extract, the seaweed oligosaccharide, the o-cymene-5-ol and the tocopherol retinoate in proper parts by weight are selected to cooperate with one another, so that the composition has better anti-pollution and antibacterial effects and has very good acne removing, repairing and soothing effects.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, in particular to an anti-pollution and acne-sensitive skin repairing composition containing golden camellia flower extract and application thereof. Background Art

[0002] Acne-prone skin is characterized by clogged pores and excessive oil secretion, often accompanied by inflammatory acne and papules, and prone to redness, swelling and uneven skin. Sensitive skin, on the other hand, is highly sensitive to external stimuli, often with discomfort such as stinging, tightness, and redness, and reacts strongly to the ingredients in skin care products. When these two skin types are combined, the resulting acne-sensitive skin puts individuals in a more serious predicament. This type of skin is prone to acne and has severe inflammation, leading to repeated attacks and no time to recover. At the same time, the superimposed sensitivity makes many ordinary skin care products unusable, resulting in a lack of effective treatment methods, increasing the psychological burden and inferiority complex, and affecting the quality of life.

[0003] Pollution from the external environment, especially air pollution, including cooking fumes (COFs), haze (PM2.5), cigarette smoke (CS), and bacteria that grow when indoor air conditioners are not cleaned for a long time, all of which cause obvious harm to acne-prone skin. Fumes produced during cooking can clog pores, and the chemicals released at the same time may cause skin inflammation and promote the formation of acne; PM2.5 particles in haze not only easily penetrate the skin, causing oxidative stress, but also degrade the skin barrier, increasing the risk of allergies; toxins in cigarette smoke can also irritate the skin, causing the reproduction of Propionibacterium acnes and worsening acne; and air conditioners that are not cleaned for a long time breed mold and bacteria, further irritating sensitive skin, causing allergies and acne to worsen. Especially for office workers who use air conditioners for cooling in summer and heating in winter, it seems that they cannot avoid this negative impact. The combined effect of these environmental factors makes the troubles of acne-prone skin more and more serious, and the double pressure makes it more difficult for the skin to recover.

[0004] At present, most products are still focused on simple anti-inflammatory and repairing, without paying attention to the importance of "protection". External pollution exists in all aspects of life, especially air pollution, which can be said to be completely unavoidable, and its harm to acne is not taken seriously. Only by repairing acne and resisting external pollution can we truly achieve effective acne removal, not only preventing acne from occurring, but also preventing existing acne from getting worse, thus restoring skin health. Summary of the invention

[0005] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide an anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract, which has anti-pollution, antibacterial, and excellent acne-removing, repairing and soothing effects, and its application.

[0006] To achieve the above-mentioned purpose, the technical solution adopted by the present invention is: a composition for anti-pollution and acne-sensitive skin repair containing Camellia chrysantha extract, comprising the following components in parts by weight: 1-5 parts of Camellia chrysantha extract, 1-5 parts of seaweed oligosaccharides, 0.01-1 parts of o-cymene-5-ol, and 0.01-1 parts of tocopherol retinoic acid ester.

[0007] Camellia chrysantha extract contains a large amount of flavonoids and polyphenols. For example, camellia glycoside A and catechins. It has significant antioxidant properties and can neutralize free radicals, thereby reducing oxidative damage caused by harmful substances in the air such as cooking fumes (COFs), haze (PM2.5) and cigarette smoke (CS). At the same time, Camellia chrysantha extract has anti-inflammatory effects, which can relieve the inflammatory response of the skin caused by environmental pollution. It also has antibacterial properties, which helps to inhibit the growth of pathogens in indoor air-conditioned environments, thereby further reducing the risk of acne formation. Seaweed oligosaccharides are a type of oligosaccharide obtained by degrading natural seaweed polysaccharides, with the advantages of low molecular weight and good water solubility. It has significant antioxidant and anti-inflammatory properties, and seaweed oligosaccharides contain many carboxyl and hydroxyl groups, which can form hydrogen bonds or electrostatic interactions with pollutants in the air, thereby promoting the adsorption of pollutants on the skin surface. This property allows seaweed oligosaccharides to capture and remove pollutants on the skin surface to a certain extent, thereby reducing their damage to the skin. Due to its hydrophilicity and adhesion, seaweed oligosaccharides can also form a protective film on the skin surface, blocking direct contact with external pollutants, preventing pollutants in the air from entering the skin and clogging pores, playing a protective and repairing role.

[0008] o-Cymene-5-ol is a water-soluble cymene with a wide range of bactericidal properties. It has a good killing effect on bacteria produced by unclean air-conditioning wind. At the same time, it can also improve the hydration of the skin, enhance the barrier function of the stratum corneum, and further resist the invasion of harmful substances from the outside world. It also has a certain effect of softening cuticles and increasing the shedding of old and waste epithelial cells, which can effectively remove acne, and can promote the penetration of effective ingredients. It has a certain whitening effect and can improve acne marks. Tocopheryl retinoic acid ester is an innovative ingredient that combines vitamin E and vitamin A. It not only has the antioxidant effect of vitamin E, but also has the beneficial effects of vitamin A on improving skin texture and inhibiting keratinization around the hair, while also having the effects of oil control and whitening. This ingredient also has significant anti-inflammatory properties, which can effectively relieve skin discomfort caused by environmental irritation or inflammation, and promote skin repair and regeneration.

[0009] The anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract of the present invention is prepared by selecting appropriate weight portions of four components, namely Camellia chrysantha extract, algae oligosaccharides, o-cymene-5-ol and tocopherol retinoic acid ester, to form a protective film, improve skin hydration, enhance the barrier function of the stratum corneum, and block direct contact with external pollutants; secondly, after a small amount of external pollutants invade, the various components work together to reduce oxidative damage caused by harmful substances in the air such as cooking fumes (COFs), haze (PM2.5) and cigarette smoke (CS); thirdly, the various components of the present invention work together to have a better anti-inflammatory effect, can effectively relieve skin discomfort caused by environmental stimulation or inflammation, and promote skin repair and regeneration.

[0010] Preferably, the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract comprises the following components in parts by weight: 2-4 parts of Camellia chrysantha extract, 2-4 parts of seaweed oligosaccharides, 0.05-0.5 parts of o-cymene-5-ol, and 0.05-0.5 parts of tocopherol retinoic acid ester.

[0011] During the actual research and development process, the inventors found that the weight proportions of the four components, camellia chrysanthemi extract, algae oligosaccharides, o-cymene-5-ol, and tocopherol retinoic acid ester, will affect the performance of the final product. When the four components, camellia chrysanthemi extract, algae oligosaccharides, o-cymene-5-ol, and tocopherol retinoic acid ester, are within the above range, the anti-pollution, antibacterial, acne-removing, and repairing and soothing effects are better.

[0012] Preferably, the weight ratio of the Camellia chrysantha extract to seaweed oligosaccharides is (1-2):1; further preferably, the weight ratio of the Camellia chrysantha extract to seaweed oligosaccharides is 1:1.

[0013] Preferably, the weight ratio of o-cymene-5-ol and tocopheryl retinoic acid ester is (1-2):1; more preferably, the weight ratio of o-cymene-5-ol and tocopheryl retinoic acid ester is 1:1.

[0014] During the actual research and development process, the inventors found that the weight ratio of Camellia chrysantha extract and seaweed oligosaccharides, and the weight ratio of o-cymene-5-ol and tocopherol retinoic acid ester in the anti-pollution and acne-repairing composition containing Camellia chrysantha extract will further affect the performance of the final product. When the weight ratio is within the above range, the anti-pollution, antibacterial, acne-removing, and repairing and soothing effects are better.

[0015] Preferably, the seaweed oligosaccharide comprises at least one of seaweed oligosaccharide-I, seaweed oligosaccharide-II, seaweed oligosaccharide-III and seaweed oligosaccharide-IV. Further preferably, the seaweed oligosaccharide is seaweed oligosaccharide-III.

[0016] The seaweed oligosaccharide of the present invention is polymerized from 4 to 9 monosaccharide units, and has a molecular weight of 500Da-1000Da. The inventors found in the actual research and development process that the seaweed oligosaccharide is polymerized from 4 to 9 monosaccharide units, and when the molecular weight is 500Da-1000Da, the short-chain oligosaccharides polymerized from 4 to 9 monosaccharide units are easier to be absorbed by the skin and can quickly penetrate into the surface of the skin. They can absorb moisture and form a protective film on the skin, improving skin feel and moisture content. Too many monosaccharide units are more suitable for forming a high-viscosity colloidal substance, which can form a stronger barrier on the skin surface to prevent water loss, and is more suitable for dry skin care, but for acne-prone skin, its skin feel can easily become a skin burden, and the effect is not what acne-sensitive skin needs most, so the acne-removing and repairing effect will be much worse.

[0017] Preferably, the preparation method of the Camellia chrysantha extract comprises the following steps: mixing Camellia chrysantha powder with ethanol aqueous solution, then extracting at 55-65°C for 25-35 minutes, then ultrasonically extracting at 55-65°C for 55-65 minutes, then concentrating the extract and drying to obtain the Camellia chrysantha extract.

[0018] Preferably, the volume percentage of ethanol in the ethanol aqueous solution is 60-80%; and / or the weight ratio of the Camellia chrysantha powder to the ethanol aqueous solution is 1:(5-20); and / or the ultrasonic power is 280-300W; and / or the temperature of the concentrated extract is 45-55°C.

[0019] Further preferably, the weight ratio of the Camellia chrysantha powder to the ethanol aqueous solution is 1:(5-10).

[0020] Preferably, the preparation method of the Camellia chrysantha powder is: collect Camellia chrysantha flowers, wash them, put them into a constant temperature oven at 60-70°C and dry them to constant weight, then grind and crush them and pass them through a 100-150 mesh sieve, collect the sieved material to obtain Camellia chrysantha powder.

[0021] In addition, the present invention provides the use of the anti-pollution and acne-sensitive skin repairing composition containing the Camellia chrysantha extract in the preparation of skin products.

[0022] Furthermore, the present invention provides a skin product, which includes the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract; the skin product is one of lotion, emulsion, cream, mask, essence, and spray.

[0023] In addition, the present invention provides a skin product, which comprises the following components in percentage by mass: 1-5% of an anti-pollution and acne-sensitive skin repairing composition containing a Camellia chrysantha extract, 1-40% of a cosmetic base, and the remainder being water.

[0024] The invention provides a skin product, which is an essence, comprising the following components in percentage by mass: 1-5% of a composition for anti-pollution and repairing acne-sensitive skin containing a golden camellia flower extract, 0.05-0.5% of a thickener, 0.5-1% of a moisturizer, 0.5-5% of an emulsifier, 0.5-3% of a preservative, 0.01-0.3% of a pH regulator, and the remainder being water.

[0025] Optionally, the essence comprises at least one of the following (a)-(e):

[0026] (a) the thickener comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol;

[0027] (b) the moisturizing agent comprises at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-propylene glycol, 1,2-butylene glycol, glycerol, budding septin polysaccharide, ceramide, and D-panthenol;

[0028] (c) the pH adjusting agent comprises at least one of arginine, tromethamine, and EDTA-disodium;

[0029] (d) the emulsifier comprises at least one of ethylhexylglycerin, dimethicone, steareth-2, steareth-2, caprylic capric triglyceride, polymethylsilsesquioxane, cetearyl glucoside, cetearyl olive oil ester, and C14-22 alcohol / C12-20 alkyl glucoside;

[0030] (e) The preservative includes at least one of 1,2-hexanediol, 1,2-pentanediol and p-hydroxyacetophenone.

[0031] Furthermore, the present invention provides a method for preparing the essence, comprising the following steps:

[0032] (1) Mix the humectant and thickener with water, heat to 85±2° C., homogenize at 1200 rpm for 4 min, and keep warm for later use to obtain prefabricated component A;

[0033] (2) After heating the emulsifier to 85±2° C., homogenize at a speed of 1200 rpm for 4 minutes. After the homogenization is completed, keep the temperature for later use to obtain the prefabricated B component;

[0034] (3) Mix the preservatives and heat to 60±2°C to melt to obtain a prefabricated C component;

[0035] (4) Component A and component B are mixed at 80° C. and homogenized at 1200 rpm for 6 min until a uniform liquid is formed. The mixture is cooled to 40° C., an anti-pollution and acne-repairing composition containing Camellia chrysantha extract is added, and stirring is continued for 8 min. Finally, a pH adjuster is added to adjust the pH to 6.0, and stirring is stopped. The mixture is discharged to obtain an essence.

[0036] Compared with the prior art, the beneficial effects of the present invention are as follows: the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract of the present invention, by selecting appropriate weight portions of four components of Camellia chrysantha extract, seaweed oligosaccharides, o-cymene-5-ol, and tocopherol retinoic acid ester to work together, first, can form a protective film, improve skin hydration, enhance the barrier function of the stratum corneum, and block direct contact with external pollutants; second, after a small amount of external pollutants invade, the various components work together to reduce oxidative damage caused by harmful substances in the air such as cooking fumes (COFs), haze (PM2.5) and cigarette smoke (CS); third, the various components of the present invention work together to have a better anti-inflammatory effect, can effectively relieve skin discomfort caused by environmental irritation or inflammation, and promote skin repair and regeneration. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 The graphs show the inhibitory effects of the compositions prepared in the examples and comparative examples on bacteria and fungi in unclean air conditioners; wherein (a) shows the inhibitory effects on bacteria in unclean air conditioners, and (b) shows the inhibitory effects on fungi in unclean air conditioners;

[0038] Figure 2 These are the effects pictures of removing acne, improving red blood streak and repairing of the essence prepared in the blank application example, implementation application example 1 and comparison application example 1. DETAILED DESCRIPTION

[0039] In order to better illustrate the purpose, technical scheme and advantages of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are the same batch of raw materials.

[0040] The raw materials used in the examples and comparative examples of the present invention are described below, but are not limited to the following raw materials:

[0041] Seaweed oligosaccharide-I, from Haisheng Health Biotechnology Co., Ltd.;

[0042] Seaweed oligosaccharide-II, from Haisheng Health Biotechnology Co., Ltd.;

[0043] Seaweed oligosaccharide-III, from Haisheng Health Biotechnology Co., Ltd.;

[0044] Seaweed oligosaccharide-IV, from Haisheng Health Biotechnology Co., Ltd.;

[0045] o-Cymene-5-ol was obtained from Guangzhou Tongjun Pharmaceutical Technology Co., Ltd.;

[0046] Tocopheryl retinoate was from Shanghai Keqin Technology Co., Ltd.

[0047] Camellia chrysantha extract 1: homemade, the preparation method is as follows: collect Camellia chrysantha flowers, wash and put them into a 65°C constant temperature oven to dry to constant weight, grind and crush them, pass them through a 150-mesh sieve, collect the sieved material to obtain Camellia chrysantha powder; add 8 times the volume fraction of 70% ethanol aqueous solution to the Camellia chrysantha powder, and extract at 60°C for 30 minutes; then extract at an ultrasonic power of 280W and a temperature of 60°C for 1 hour; filter after the extraction, collect the extract, and concentrate the extract by rotary evaporation at 50°C. After the concentration, place it in an ultra-low temperature freeze dryer to dry and freeze, and obtain Camellia chrysantha extract 1;

[0048] Camellia chrysantha extract 2: homemade, the preparation method is as follows: collect Camellia chrysantha flowers, wash and put them into a 65°C constant temperature oven to dry to constant weight, grind and crush them, pass them through a 100-mesh sieve, collect the sieved material to obtain Camellia chrysantha powder; add 15 times the volume fraction of 65% ethanol aqueous solution to the Camellia chrysantha powder, and extract at 65°C for 25 minutes; then extract at an ultrasonic power of 290W and a temperature of 55°C for 1 hour; filter after the extraction, collect the extract, and concentrate the extract by rotary evaporation at 45°C. After the concentration, place it in an ultra-low temperature freeze dryer to dry and freeze, and obtain Camellia chrysantha extract 2;

[0049] Camellia chrysantha extract 3: homemade, the preparation method is as follows: collect Camellia chrysantha flowers, wash and put them into a 65°C constant temperature oven to dry to constant weight, grind and crush them, pass them through a 100-mesh sieve, collect the sieved material to obtain Camellia chrysantha powder; add 20 times the volume fraction of 80% ethanol aqueous solution to the Camellia chrysantha powder, and extract at 60°C for 30 minutes; then extract at an ultrasonic power of 300W and a temperature of 65°C for 1 hour; filter after the extraction, collect the extract, and concentrate the extract by rotary evaporation at 55°C. After the concentration, place it in an ultra-low temperature freeze dryer to dry and freeze, and obtain Camellia chrysantha extract 3;

[0050] Camellia extract, manufacturer Jiangsu Jufeng Chemical Technology Co., Ltd.;

[0051] Xinqiong hexasaccharide, manufactured by Blue Brain Technology (Xiamen) Co., Ltd., is composed of 6 monosaccharide units;

[0052] Totarol, manufacturer: Tesco Chemical (Hubei) Co., Ltd.;

[0053] Bakuchiol, manufactured by Shaanxi Fuheng Biotechnology Co., Ltd.;

[0054] Examples and Comparative Examples

[0055] The present invention provides an anti-pollution and acne-sensitive skin repairing composition containing camellia chrysantha extract. The components and weight portions of the anti-pollution and acne-sensitive skin repairing composition containing camellia chrysantha extract are shown in Tables 1 and 2.

[0056] Table 1

[0057]

[0058]

[0059] Table 2

[0060]

[0061] Table 3

[0062]

[0063]

[0064] Application Examples 1-10 and Comparative Application Examples 1-8 use the compositions of Examples 1-10 and Comparative Examples 1-8 respectively, Application Example 1 uses the composition of Example 1, one to one, and Application Example 11 uses the composition corresponding to Example 1.

[0065] The present invention provides a method for preparing the essence, comprising the following steps:

[0066] (1) Mix the humectant and thickener with water, heat to 85±2° C., homogenize at 1200 rpm for 4 min, and keep warm for later use to obtain prefabricated component A;

[0067] (2) After heating the emulsifier to 85±2° C., homogenize at a speed of 1200 rpm for 4 minutes. After the homogenization is completed, keep the temperature for later use to obtain the prefabricated B component;

[0068] (3) Mix the preservatives and heat to 60±2°C to melt to obtain a prefabricated C component;

[0069] (4) Component A and component B are mixed at 80° C. and homogenized at 1200 rpm for 6 min until a uniform liquid is formed. The mixture is cooled to 40° C., an anti-pollution and acne-repairing composition containing Camellia chrysantha extract is added, and stirring is continued for 8 min. Finally, a pH adjuster is added to adjust the pH to 6.0, and stirring is stopped. The mixture is discharged to obtain an essence.

[0070] The preparation methods of the remaining application examples, comparative application examples, and blank application examples are the same as that of application example 1, except that no relevant components are added if they are not present.

[0071] Performance Test-1 The inhibitory effect of the composition on external cooking fumes (COFs), haze (PM2.5) and cigarette smoke (CS).

[0072] Pollutants in the air are one of the main causes of recurrent acne and skin sensitivity in people with acne-sensitive skin. These pollutants can induce keratinocyte death, causing the skin condition to become worse. Therefore, by measuring the effect of the embodiment and the comparative example on the activity of keratinocytes containing pollutants, if the cell activity is improved compared with the group containing only pollutants, it proves that the composition has an anti-pollution effect, and the stronger the cell activity, the better the anti-pollution effect.

[0073] Experimental methods: Haze particles (PM2.5) were collected for 24 hours using a Th-150c III mid-flow atmospheric sampler with a quartz filter membrane; smoking volunteers were recruited to generate cigarette smoke in a closed room, and the smoke (CS) was collected using an atmospheric sampler with a quartz filter membrane; cooking fume (COF) was also collected using an atmospheric sampler with a quartz filter membrane during the cooking process. The samples were immediately stored at -20°C after collection.

[0074] The quartz filter of the atmospheric sampler containing these air pollutants was then cut into pieces and extracted by microwave ultrasound using n-hexane / dichloromethane (1 / 1, v / v) for 24 h. The organic extract was concentrated to about 1 mL using a rotary evaporator, and the pollutants were diluted with dimethyl sulfoxide (DMSO) and stored at -20 °C.

[0075] Human keratinocytes HaCaT were revived and cultured for 24 hours using MEM containing 10% FBS as a culture medium. The anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract of the embodiments and comparative examples of the present invention was diluted to 2% (v / v) using DMSO. The cells in the sample group were treated with PM2.5 (100 μg / mL), CS (100 μg / mL) and COF (10 mg / mL) stimulant solutions and solutions of each embodiment and comparative example for 24 hours, and the control group was only treated with PM2.5, CS and COF stimulant solutions for 24 hours. Different compositions were configured as sample solutions and co-cultured with HaCaT cells, and the cells were harvested. The cell activity of HaCaT cells in the sample group and the control group was detected using the MTT kit method.

[0076] Cell activity improvement rate (%) = (cell activity of sample group - cell activity of control group) / cell activity of control group × 100%; the obtained results are shown in Table 4.

[0077] Table 4

[0078]

[0079]

[0080] It can be seen from the above table that the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract prepared in the embodiment of the present invention has an improvement rate of cellular activity of PM2.5, CS and COF of ≥20%.

[0081] From the comparison of Examples 1-5, it can be seen that the weight ratio of Camellia chrysantha extract and seaweed oligosaccharides, and the weight ratio of o-cymene-5-ol and tocopherol retinoic acid ester in the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract will further affect the performance of the final product. When the weight ratio of the Camellia chrysantha extract and seaweed oligosaccharides is (1-2):1; and / or the weight ratio of o-cymene-5-ol and tocopherol retinoic acid ester is (1-2):1, the cell activity improvement rate of PM2.5, CS, and COF is better. When the weight ratio of the Camellia chrysantha extract and seaweed oligosaccharides is 1:1; and / or the weight ratio of o-cymene-5-ol and tocopherol retinoic acid ester is 1:1, the cell activity improvement rate of PM2.5, CS, and COF reaches the best.

[0082] From the comparison of Example 1 and Examples 6-7, it can be seen that the extraction method of Camellia chrysantha will affect the cell activity improvement rate of PM2.5, CS, and COF. When the weight ratio of Camellia chrysantha powder to ethanol aqueous solution is 1: (5-10), the cell activity improvement rate of PM2.5, CS, and COF is better.

[0083] From the comparison between Example 1 and Examples 8-10, it can be seen that the type of the seaweed oligosaccharide will affect the improvement rate of cell activity for PM2.5, CS, and COF. When the seaweed oligosaccharide is seaweed oligosaccharide-III, the improvement rate of cell activity for PM2.5, CS, and COF is better.

[0084] From the comparison of Example 1 and Comparative Examples 1-8, it can be seen that the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract of the present invention comprises four components, namely Camellia chrysantha extract, algae oligosaccharides, o-cymene-5-ol and tocopherol retinoic acid ester, which work synergistically and are indispensable.

[0085] Performance Test-2 Inhibitory effect of the composition on bacteria and fungi in dirty air conditioners.

[0086] Common bacteria in unclean air conditioners include Staphylococcus, Escherichia coli, and molds, such as Penicillium and black mold. These microorganisms multiply in a humid environment, releasing allergens and toxins, which may lead to increased skin inflammation and the formation of acne. For acne-sensitive skin, the presence of bacteria and fungi will aggravate sebum secretion, induce pore blockage and infection, thereby causing acne and skin discomfort, leading to worsening skin conditions and increasing the risk of recurrence of acne. The antibacterial and anti-pollution capabilities of the composition can be judged by the killing effect of the composition on harmful bacteria and fungi in the air conditioner. The stronger the killing effect, the stronger the anti-pollution ability.

[0087] Experimental method: Prepare meat extract peptone liquid medium (LB medium), prepare potato glucose broth (PDB medium), wipe the fan blades of the dirty air conditioner with a disposable cotton swab, and put them into LB medium and PDB medium respectively. LB medium is used to culture the bacteria contained in the air conditioner for 48 hours, and PDB medium is used to culture the fungi contained in the air conditioner for 48 hours to form a bacterial solution. The composition of the embodiment and comparative example is prepared into a 1% sample solution with DMSO for standby use. 180 μL of bacterial solution is added to a 96-well plate, and then 40 μL of each composition is added. The control group is set without adding the composition. After culturing for 24 hours, the OD value of each well is measured with an enzyme marker. The smaller the OD value, the clearer the liquid, the worse the activity of the bacteria, and the better the antibacterial effect of the composition.

[0088] Inhibition rate (%) = (OD value of control group - OD value of sample group) / OD value of control group × 100%; the results are shown in Table 5.

[0089] Table 5

[0090]

[0091]

[0092] It can be seen from the above table that the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract prepared in the embodiment of the present invention has an inhibition rate of ≥60% on bacteria and fungi in unclean air conditioners. Figure 1 The figures show the inhibitory effects of the compositions prepared in the examples and comparative examples on bacteria and fungi in unclean air conditioners; wherein, (a) is a figure showing the inhibitory effects on bacteria in unclean air conditioners, and (b) is a figure showing the inhibitory effects on fungi in unclean air conditioners, respectively presenting the inhibitory effects of the anti-pollution and acne-sensitive skin repairing compositions containing Camellia chrysantha extract prepared in Examples 1-10 and Comparative Examples 1-8 on bacteria and fungi.

[0093] From the comparison of Examples 1-5, it can be seen that the weight ratio of Camellia chrysantha extract and seaweed oligosaccharides, and the weight ratio of o-cymene-5-ol and tocopherol retinoic acid ester in the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract will further affect the performance of the final product. When the weight ratio of the Camellia chrysantha extract and seaweed oligosaccharides is (1-2):1; and / or the weight ratio of o-cymene-5-ol and tocopherol retinoic acid ester is (1-2):1, the inhibition rate of bacteria and fungi in unclean air conditioners is better. When the weight ratio of the Camellia chrysantha extract and seaweed oligosaccharides is 1:1; and / or the weight ratio of o-cymene-5-ol and tocopherol retinoic acid ester is 1:1, the inhibition rate of bacteria and fungi in unclean air conditioners reaches the best.

[0094] From the comparison of Example 1 and Examples 6-7, it can be seen that the extraction method of Camellia chrysantha will affect the inhibition rate of bacteria and fungi in unclean air conditioners. When the weight ratio of Camellia chrysantha powder to ethanol aqueous solution is 1: (5-10), the inhibition rate of bacteria and fungi in unclean air conditioners is higher.

[0095] From the comparison between Example 1 and Examples 8-10, it can be seen that the type of seaweed oligosaccharide has little effect on the inhibition rate of bacteria and fungi in unclean air conditioners.

[0096] From the comparison of Example 1 and Comparative Examples 1-8, it can be seen that the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract of the present invention comprises four components, namely Camellia chrysantha extract, algae oligosaccharides, o-cymene-5-ol and tocopherol retinoic acid ester, which work synergistically and are indispensable.

[0097] Performance Test-3 The composition has acne-removing, repairing and soothing effects on acne-sensitive skin.

[0098] This effect example tests the improvement effects of the application example, comparative application example and blank application example on human skin TEWL, acne count and red blood streak, and respectively detects the effects of the essence prepared by the application example of the present invention on barrier repair, acne removal and sensitivity relief of acne-sensitive skin.

[0099] The specific test method is as follows: According to the "Technical Specification for Safety of Cosmetics" (2015), volunteers with sensitive skin aged 18-50 (lactic acid stinging score > 3) were selected and randomly divided into 20 groups, with 5 people in each group. The volunteers had obvious acne, blackheads, and papules. Under normal circumstances, the volunteers continued to use the product on their entire face for 14 days; every morning and evening, after cleansing and moisturizing, the volunteers applied an appropriate amount of essence on the face and massaged for absorption. The acne area can be overlapped and the entire face is used once in the morning and evening, and a follow-up visit was conducted after 14 days.

[0100] On the day of the volunteer visit, the volunteers washed their faces with water in the test area without applying any products, and sat in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 20 minutes. The improvement of facial acne indicators was quantified and photographed using instruments, including acne counting (counting the total number of comedones and papules, using VISIA and doctor evaluation), Tewameter TM Hex test for transepidermal water loss TEWL, and MX 18 test for red blood streaks.

[0101] Improvement rate before and after use = |mean value before use - mean value after use| / mean value before use × 100%; the calculation results are shown in Table 6. The improvement rate presented in Table 6 is the improvement rate of the follow-up visit on the 14th day.

[0102] Table 6

[0103]

[0104]

[0105] It can be seen from the above table that the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract prepared in the embodiment of the present invention has a good effect of improving acne, repairing and soothing, and improving red blood streaks when applied to essence. Figure 2 These are the effects pictures of removing acne, improving red blood streak and repairing of the essence prepared in the blank application example, implementation application example 1 and comparison application example 1.

[0106] From the comparison of Examples 1-5, it can be seen that the weight ratio of Camellia chrysantha extract and seaweed oligosaccharides, and the weight ratio of o-cymene-5-ol and tocopherol retinoic acid ester in the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract will further affect the performance of the final product. When the weight ratio of the Camellia chrysantha extract and seaweed oligosaccharides is (1-2):1; and / or the weight ratio of o-cymene-5-ol and tocopherol retinoic acid ester is (1-2):1, the effects of improving acne, repairing and soothing, and improving red blood streaks are better. When the weight ratio of the Camellia chrysantha extract and seaweed oligosaccharides is 1:1; and / or the weight ratio of o-cymene-5-ol and tocopherol retinoic acid ester is 1:1, the effects of improving acne, repairing and soothing, and improving red blood streaks are best.

[0107] From the comparison of Example 1 and Examples 6-7, it can be seen that the extraction method of Camellia chrysantha will affect the effects of improving acne, repairing and soothing, and improving red blood streaks. When the weight ratio of Camellia chrysantha powder to ethanol aqueous solution is 1: (5-10), the effects of improving acne, repairing and soothing, and improving red blood streaks are better.

[0108] From the comparison between Example 1 and Examples 8-10, it can be seen that the type of the seaweed oligosaccharide will affect the effects of improving acne, repairing and soothing, and improving red blood streaks. When the seaweed oligosaccharide is seaweed oligosaccharide-III, the effects of improving acne, repairing and soothing, and improving red blood streaks are better.

[0109] From the comparison of Example 1 and Comparative Examples 1-8, it can be seen that the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract of the present invention comprises four components, namely Camellia chrysantha extract, algae oligosaccharides, o-cymene-5-ol and tocopherol retinoic acid ester, which work synergistically and are indispensable.

[0110] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A composition containing golden camellia extract for anti-pollution and acne-sensitive skin repair, characterized in that: The invention comprises the following components in parts by weight: 1-5 parts of golden camellia extract, 1-5 parts of seaweed oligosaccharide, 0.01-1 parts of o-cymene-5-ol and 0.01-1 parts of tocopherol retinoic acid ester.

2. The anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 2-4 parts of golden camellia extract, 2-4 parts of seaweed oligosaccharide, 0.05-0.5 parts of o-cymene-5-ol and 0.05-0.5 parts of tocopherol retinoic acid ester.

3. The anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract according to claim 1, characterized in that: The weight ratio of the Camellia chrysantha extract to seaweed oligosaccharide is (1-2):

1.

4. The anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract according to claim 1, characterized in that: The weight ratio of o-cymene-5-ol and tocopheryl retinoic acid ester is (1-2):

1.

5. The anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract according to claim 1, characterized in that: The seaweed oligosaccharide includes at least one of seaweed oligosaccharide-I, seaweed oligosaccharide-II, seaweed oligosaccharide-III and seaweed oligosaccharide-IV.

6. The anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract according to claim 1, characterized in that: The preparation method of the camellia chrysantha extract comprises the following steps: mixing camellia chrysantha powder with ethanol aqueous solution, then extracting at 55-65°C for 25-35min, then ultrasonically extracting at 55-65°C for 55-65min, then concentrating the extract and drying to obtain the camellia chrysantha extract.

7. The anti-pollution and acne-sensitive skin repairing composition containing the Camellia chrysantha extract according to claim 6, characterized in that: In the ethanol aqueous solution, the volume percentage of ethanol is 60-80%; And / or, the weight ratio of the Camellia chrysantha powder to the ethanol aqueous solution is 1:(5-20); And / or, the ultrasonic power is 280-300W; And / or, the temperature of the concentrated extract is 45-55°C.

8. Use of the anti-pollution and acne-sensitive skin repairing composition containing the Camellia chrysantha extract as claimed in any one of claims 1 to 7 in the preparation of skin products.

9. A skin care product, characterized in that: The skin product comprises the anti-pollution and acne-sensitive skin repairing composition containing Camellia chrysantha extract as claimed in any one of claims 1 to 7; the skin product is one of lotion, emulsion, cream, mask, essence and spray.

10. The skin care product according to claim 9, wherein: The skin product comprises the following components in percentage by mass: 1-5% of the anti-pollution and acne-sensitive skin repairing composition containing golden camellia extract, 1-40% of the cosmetic base, and the remainder of water.

Images