152 results about "Tea catechin" patented technology

The invention relates to the technical field of food processing, and discloses casein-tea polysaccharide-polyphenol composite nanoparticles and a preparation method of the casein-tea polysaccharide-polyphenol composite nanoparticles, and the preparation method comprises the following steps: mixing large yellow tea polysaccharide with a casein solution to obtain a CS / TPS mixed solution; performing glycosylation modification on casein in the CS / TPS mixed solution by adopting a Maillard reaction to prepare a casein-polysaccharide copolymer solution; the preparation method comprises the following steps: mixing a plurality of catechins, dissolving the catechins in ultrapure water to obtain a mixture stock solution, mixing the mixture stock solution and a casein-polysaccharide copolymer solution according to a volume ratio of 1: 16, stirring for 1 hour for self-assembly, pre-freezing the stirred solution at-80 DEG C, and freeze-drying for 48 hours to obtain CS-TPS-MIX nano-composite powder. According to the invention, a polyphenol delivery system with a dual antioxidant synergistic mechanism is constructed through a Maillard reaction mediated nano-composite technology, and the system is stable in structure, so that the release of polyphenol is more controllable.

The invention provides a dendrobium devonianum epiphytic tea tree cultivation method, which belongs to an epiphytic plant planting technology, and is characterized in that a dendrobium devonianum root system can be closely fused with a tea tree trunk through treatment of'minimal trauma + callus inducer ', and a structural foundation is laid for material exchange. The jasmonic acid methyl ester stress with a specific concentration is applied to the dendrobium nobile in the key phenological period, the dendrobium nobile is accurately induced to synthesize and secrete beneficial secondary metabolites, the theanine content in the tea can be remarkably increased, the proportion of ester type catechin can be reduced, and therefore the fresh degree of the tea is remarkably increased, and the astringent taste of the tea is remarkably reduced. The stress resistance of the dendrobium devonianum and the tea trees treated by the method is also enhanced, and the disease occurrence rate is reduced. While high-quality dendrobium nobile is harvested, the quality and economic benefits of the produced tea leaves are synchronously improved.

The invention relates to composite cosmetic nanoparticles with an acne removing effect and a preparation method of the composite cosmetic nanoparticles, and belongs to the technical field of functional skin care products and nano entrapment. According to the nano-particles, low-molecular chitosan is used as a main carrier, and natural multifunctional acne-removing components including a white willow bark extract, a tea catechin substance, a pomegranate peel extract and a hovenia dulcis thunb fruit extract are jointly embedded, so that a stable nano-particle structure with the particle size of 50-200 nm is formed; and sodium hyaluronate is used for surface modification so as to enhance skin adhesiveness and permeability. The multi-target synergistic active component can effectively inhibit the growth of propionibacterium acnes, relieve inflammatory response, regulate sebum secretion and enhance the antioxidant repair capacity of skin. The nano-particles prepared by the invention are concentrated in particle size distribution, high in entrapment rate and good in biocompatibility, are suitable for essence, spray, mask type and gel type acne-removing skin care products, and show excellent product transformation potential.

The invention discloses a polydopamine-tea polyphenol coated copper manganese oxide composite nano-enzyme as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. According to the invention, a DA-Cu (II)-EGCG (epigallocatechin gallate) complex is formed by virtue of chelation of main components of dopamine and tea polyphenol, namely epigallocatechin gallate, and copper ions; potassium permanganate is introduced for oxidative polymerization to obtain the polydopamine-tea polyphenol / copper manganese oxide organic-inorganic hybrid nano composite material, the nano composite material is monodisperse nano particles, the average particle size is about 80 nm, the nano composite material shows excellent nano enzyme catalytic activity, active oxygen free radicals can be effectively removed, and the nano composite material can be used for preparing nano-enzyme. The material has good photo-thermal conversion performance under near-infrared light, and shows excellent photo-thermal and photodynamic synergistic antibacterial effects. The preparation method disclosed by the invention is simple, mild, environment-friendly, low in cost and convenient for batch production.

An object of the present invention is to provide a tea beverage having both a tea-like taste and a fruity aroma while containing tannin and catechin at low concentrations, a method for producing the same, and the like.SOLUTION: A tea beverage having a tannin concentration of 330mg / L or less, a caffeine concentration of 80mg / L or less, and a fruity aroma, wherein the tea beverage satisfies at least one of the following conditions (i) and (ii): (i) a roseoxide concentration of 0.0001 to 5ppm; and (ii) a citronellol concentration of 0.0001 to 10ppm.SELECTED DRAWING: None

The invention provides application of tea catechin in preparation of a medicine for preventing and treating bortezomib-induced peripheral neuropathy. A plurality of in-vitro model results show that tea catechin can be used as a copper death inhibitor, remarkably improves related indexes of bortezomib-induced peripheral neuropathy, improves survival rates of Schwanton cells and SH-SY5Y, improves mitochondrial functions and cell cycle abnormalities of the Schwanton cells, reduces intracellular copper ion, cuprous ion and ATP levels, and can be used for preparing the copper death inhibitor for the peripheral neuropathy of the Bortezomib-induced peripheral neuropathy of the Bortezomib-induced peripheral neuropathy. Meanwhile, the death of Schwann cells and SH-SY5Y induced by the copper death inducer is reduced. In-vivo experiments show that the tea catechin plays a role in protecting functional damage of the bortezomib, and the tea catechin remarkably improves mechanical hyperalgesia, gait abnormality and pathological changes of myelin sheath and axon of mice caused by peripheral neuropathy induced by the bortezomib. The tea catechin can be used as a medicine for peripheral neuropathy induced by bortezomib, and has high clinical application value and development prospect.

The invention discloses a processing method for increasing the content of methylated catechin in white tea. The processing method sequentially comprises the following steps: 1) picking fresh tea leaves; (2) properly withering the fresh leaves; according to the processing method, the content of methylated catechin in the white tea is effectively increased, so that the content of methylated catechin in the white tea is remarkably increased by 1.5-5 times, and the processing method has the advantages of being easy and convenient to operate, low in cost and the like and has wide application prospects in the field of white tea processing.

The invention discloses an areca-nut solid fertilizer for improving the content of catechin and precise nutrition of areca-nuts and application of the areca-nut solid fertilizer, and belongs to the technical field of fertilizer preparation. The preparation method comprises the following steps: mixing cassava straws, cassava residues, hermetia illucens sand, calcium superphosphate, quick lime and zymophyte, and carrying out first composting fermentation to obtain a first fermented product; and adding macroelements containing monopotassium phosphate, potassium nitrate, urea, calcium carbonate and magnesium sulfate in a specific proportion, microelements containing boron, zinc, copper, iron and manganese in a specific proportion and bacillus subtilis into the first fermented product, carrying out second composting fermentation, and carrying out aging treatment to obtain the betel nut solid fertilizer. The prepared betel nut solid fertilizer can accurately provide nutrients according to the requirements of betel nuts for various nutrient elements, and negative effects on soil due to excessive nutrient elements are avoided; meanwhile, the content of areca catechin is increased, and the nutritional value of the areca catechin is enhanced.

The invention provides a method for judging the grade of Zhenmei green tea based on chemical components and a regression equation, and belongs to the technical field of agricultural product quality detection.The method comprises the steps that data of four target chemical components in to-be-detected Zhenmei green tea is obtained, comprising 5-gallic quinic acid, epigallocatechin gallate, gallocatechin gallate and epicatechin gallate, and is prepared from 5-gallic quinic acid, epigallocatechin gallate, gallocatechin gallate and epicatechin gallate; the data of the target chemical components are input into a regression equation, a prediction grade value corresponding to the to-be-detected Zhenmei green tea is obtained through calculation, the four target chemical components are obtained through screening based on data of known different grade sample sets, and the prediction grade value of the to-be-detected Zhenmei green tea is obtained. The regression equation is obtained through ridge regression analysis training based on data and quality grades of four target chemical components of known different-grade sample sets. The method is used for solving the problem that the grade of tea is inaccurately judged through sense organs at present, and the Zhenmei green tea grade judgment method completely based on quantifiable chemical components is achieved.

The invention provides a process for processing eurotium cristatum-containing Fuzhuan tea from Zijuan tea, which comprises the following steps of: preparing raw materials, namely taking sun-dried raw Yunnan Zijuan tea as a raw material; the raw materials are signed with a long-term purchasing contract signed by the Yunnan theaceae, and a Zijuan tea growth soil detection report and a Zijuan tea detection report are provided by the Yunnan theaceae. S2, the Zijuan tea is subjected to pile fermentation, steam compression molding, eurotium cristatum growing, drying, finished product obtaining and the like in sequence, the components of the Zijuan tea comprise anthocyanin, eurotium cristatum, tea polyphenol, catechin and amino acid powder, the Zijuan tea contains high-content anthocyanin and various active ingredients beneficial to the human body, and the eurotium cristatum is introduced, so that the health care effect of the Zijuan tea is improved; the tea leaves have various health-care functions of reducing blood pressure, reducing blood sugar, reducing uric acid, losing weight and the like, can also be blended with other tea, and through special fermentation and cultivation processes, the golden Zijuan tea has unique medical and pharmacological effects, and the Zijuan Fuzhuan tea has unique taste and fragrance.

The invention discloses a preparation method and application of a functional tea extract, and aims to solve the problems that active ingredients are easy to oxidize and degrade and low in purity in existing tea leaf processing. Enzymatic browning is inhibited through liquid nitrogen quick-freezing pretreatment, the mass transfer efficiency is enhanced in combination with dynamic ultrasonic-assisted extraction, efficient enrichment is achieved by adopting a multi-stage membrane separation or resin purification technology, and the retention rate and purity of catechin substances (especially epigallocatechin gallate EGCG) are remarkably improved. According to the method, thermal degradation and oxidation loss are effectively reduced, and the obtained extract has excellent antioxidant and antibacterial properties and can be widely applied to the fields of functional food, health care products and the like.

To provide an adzuki bean-derived food material including a bile acid micelle dissolubility inhibiting action and a bile acid binding insolubilized material forming action derived from adzuki beans, and to provide a production method of the same.SOLUTION: An adzuki bean-derived food material as a food material derived from Vigna angularis contains (+)-catechin 7-O-β-D-glucopyranoside, (+)-epicatechin 7-O-β-D-glucopyranoside and (+)-catechin, and includes a bile acid micelle dissolubility inhibiting action and a bile acid binding insolubilized matter forming action. A production method of the adzuki bean-derived food material includes the steps of: an adzuki bean immersion step of immersing adzuki beans in ethanol-containing water; a liquid matter collecting step of removing adzuki beans from the immersion water after the adzuki bean immersion step, and collecting a liquid matter; and a drying step of drying the liquid matter obtained in the liquid matter collecting step so as to obtain a dried matter.SELECTED DRAWING: None

This invention relates to the field of intelligent control technology for tea processing, specifically to an adaptive optimization control method and system for tea processing parameters. The method involves performing near-infrared spectral scanning and hyperspectral imaging on incoming fresh leaves to obtain feature vectors; using a reaction kinetic model combining catechin enzymatic oxidation, chlorophyll demagnesiation, and amino acid thermal degradation with a data-driven residual correction network to calculate the state vectors of internal components; and having a near-end strategy optimization agent output the combined adjustment values ​​for the withering hot air temperature, rolling speed, and drying air inlet temperature, which are then sent to the processing equipment controller for execution. Through cross-process quality state transmission, the withering, rolling, and drying processes are linked for coordinated control. This invention dynamically tracks the state of internal components at the chemical reaction level, solving the technical problem of large quality fluctuations under heterogeneous fresh leaf conditions in existing lookup-based process parameter schemes.

The invention relates to the technical field of tea leaf processing, and discloses a tea leaf extraction process with a catechin retention rate, which comprises the following steps: S1, crushing tea leaves; s2, carrying out enzymolysis by adopting a compound enzyme solution; s3, adding the eutectic solvent; s4, performing ultrasonic-assisted extraction under the protection of nitrogen; s5, performing centrifugal separation to obtain a deep-eutectic solvent extracting solution, and adsorbing catechin in the deep-eutectic solvent extracting solution through a macroporous adsorption resin column; s6, removing impurities; and S7, carrying out vacuum rotary evaporation, and drying to obtain tea polyphenol powder with high catechin content. Compared with the prior art, the method has the advantages that the dissolution rate of the catechin is increased from the source through composite enzymolysis and mild deep-eutectic solvent extraction, fine separation is realized at normal temperature by utilizing a multi-stage membrane technology, and degradation and isomerization of the catechin caused by heat and chemical factors are avoided to the greatest extent. The total content of catechin in a final product is effectively improved.

The invention provides a fat-reducing nutritional composition containing a kelp extract and a preparation method of the fat-reducing nutritional composition, belongs to the technical field of foods, and relates to fermentation engineering and marine food manufacturing. The fat-reducing nutritional composition comprises the following components: a kelp extract, opuntia ficus-indica powder, an enhancer, a flavoring agent and a nutritional agent, wherein the enhancer is prepared from calcium beta-hydroxy-beta-methyl butyrate, catechin and betaine. The fat-reducing nutritional composition disclosed by the invention is scientific in compatibility of the components, can effectively regulate catabolism of fat, improve fat consumption, reduce fat accumulation, timely supplement nutrition, prevent muscle loss, synchronously improve the metabolic level and immunity of a body, and achieve the effects of health and fat reduction.

The application provides a method for preparing epigallocatechin gallate (EGCG) by using a dynamic axial compression column. The method comprises the following steps: taking a tea polyphenol extract as a raw material, and separating and purifying EGCG by using a dynamic axial compression column. The method can safely and efficiently prepare high-purity EGCG.

The invention relates to a novel beverage and a preparation method thereof. Comprising the following components in percentage by weight: 0.05%-0.6% of a double-petal red rose distillation extracting solution, 0.05%-0.6% of food-grade hyaluronic acid, 0.05%-0.6% of xylitol or erythritol, 0.1%-0.3% of a butterfly bean flower extracting solution, 0.05%-0.2% of a turmeric root extracting solution, 0.03%-0.1% of mint leaf essence, 0.1%-0.4% of chia seed powder, 0.2%-0.5% of aloe juice, 0.1%-0.3% of a pomegranate extracting solution, 0.05%-0.2% of a green tea extracting solution, 0.02%-0.1% of citric acid and the balance of water. Hyaluronic acid and rose hydrolat are added, so that the oral liquid has the effects of moisturizing, resisting aging, soothing liver-qi stagnation and relieving and reducing pressure after being orally taken, aloe polysaccharide and chia seeds form a stable colloidal network, satiety and skin repair capacity are improved, low sugar and low burden are achieved, pomegranate ellagic acid has the effects of resisting inflammation, natural color matching with butterfly bean flowers is achieved, visual interest is enhanced, green tea catechin and curcumin are compounded to resist oxidation, promote metabolism and strengthen immunity, and the oral liquid has the effects of promoting metabolism and improving immunity. And the defects of irritation, single nutrition and the like of the traditional beverage are overcome.

The invention provides a pharmaceutical composition containing oridonin, a pharmaceutical preparation containing the oridonin and application of the pharmaceutical preparation. The pharmaceutical composition comprises the oridonin and epigallocatechin gallate. By taking breast cancer cells MCF-7 as an in-vitro experimental model, the invention finds that compared with a control group (single medication), the composition of oridonin serving as a main component in rabdosia rubescens and epigallocatechin gallate serving as a main component in polyphenols in tea leaves has the advantages that in a specific ratio range, the content of the oridonin serving as the main component in the rabdosia rubescens and the content of the epigallocatechin gallate serving as the main component in the polyphenols in the tea leaves can be greatly stronger proliferation inhibition activity is shown on breast cancer cells. The compound can generate a synergistic inhibition effect on cancer treatment, and is suitable for preparing antitumor drugs.

A polyphenol-rich composition is orally taken to alleviate a symptom or symptom of a post-viral infection syndrome, and preferably post-COVID syndrome. Most typically, the composition comprises at least 50 wt% of total catechins, at least 20 wt% of total chlorogenic acids, and optionally up to 30 wt% of total supplemental antioxidants. The composition may be formulated from a green coffee bean extract, a green tea extract, a turmeric extract, sour cherries or an extract thereof, broccoli or an extract thereof, and kale or an extract thereof. It is worthy of notice that such compositions are effective in reducing proinflammatory cytokines and interleukinins, increasing NOHb, and reducing reactive oxygen species caused by mitochondrial dysfunction, iNOS activity, and NOX2 activity in individuals infected with SARS-CoV2.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker combination for auxiliary selection of a high-ester catechin tea tree and application thereof, and belongs to the technical field of molecular markers. The SNP molecular marker combination provided by the invention comprises three sites, namely an SNP1 (Single Nucleotide Polymorphism) located at Chr2: 197057556, an SNP2 located at Chr4: 36953566 and an SNP3 located at Chr11: 115194214; by detecting the genotypes of the SNP loci in a tea tree sample, a tea tree single plant with high ester catechin (CG, GCG and EGCG) content can be predicted and screened early, quickly and accurately, the breeding efficiency of a tea tree variety with high ester catechin content is remarkably improved, and the method has an excellent application prospect.

The invention provides a preparation method of fructus aurantii immaturus and paeonia lactiflora powder of a classic famous formula The invention also provides a method for detecting the quality of the fructus aurantii immaturus and paeonia lactiflora powder prepared by the preparation method of the classic famous prescription fructus aurantii immaturus and paeonia lactiflora powder. The invention establishes a preparation process of the immature bitter orange and Chinese herbaceous peony powder preparation, and particularly, a process route and key process parameters obtained through systematic research on immature bitter orange charcoal frying process parameters in the formula not only accord with description of the medicine in ancient books, but also can meet actual equipment conditions of modern large-scale production. Within the determined key process parameter range, the decrease amplitude of three heat-sensitive components, namely catechin, narirutin and naringin, which are easy to decompose after being heated in the immature bitter orange charcoal can be controlled within 10%, so that the effectiveness and the quality controllability of the product are ensured. The UPLC method established by the invention can more quickly realize simultaneous detection of eight components in the immature bitter orange and Chinese herbaceous peony powder, can be used as an evaluation method for the process research, and can also be used for controlling the product quality in the production of the immature bitter orange and Chinese herbaceous peony powder.

The invention relates to the technical field of plant biology, and discloses a method for improving stress resistance of secondary metabolites of tea trees. The method comprises the following steps: collecting multi-omics time sequence data of tea trees under drought stress, and constructing a time-space aligned multi-dimensional data matrix; modeling a dynamic causal regulation relationship among genes, metabolites and phenotypes by utilizing a dynamic Bayesian network; designing a multi-dimensional evaluation algorithm fusing network topology and biological effect to screen key regulatory genes; the influence on target metabolites such as catechin is quantitatively predicted through in-vivo computer disturbance simulation; and finally generating an optimal gene editing or molecular marker assisted breeding scheme. According to the method, breakthrough from correlation analysis to causal inference is achieved, and the accuracy and efficiency of collaborative improvement of the stress resistance and the secondary metabolites of the tea trees are improved.

This invention discloses a method for determining the origin of tea based on the stable isotope ratio of EGCG monomers, belonging to the field of tea origin identification technology. It aims to solve the technical problem of insufficient accuracy in existing tea origin identification methods. The method involves measuring the overall stable isotope ratio δ in the tea sample. 13 C 茶叶 δ 15 N 茶叶 δ 2 H 茶叶 δ 18 O 茶叶 and the δ¹² value of stable isotope ratios of EGCG monomers 13 C EGCG δ 2 H EGCG and δ 18 O EGCG The preparation method of EGCG monomer involves making tea powder from tea samples, extracting and purifying epigallocatechin gallate (EGCG) monomer from the tea powder, and then determining the stable isotope ratio of the EGCG monomer. Based on the above stable isotope ratio, the origin of the tea sample is identified. This invention is mainly used for the accurate identification of tea origin, providing technical support for tea traceability and quality control.

This invention provides a ready-to-drink milk tea based on a ternary complex stabilized with κ-casein genotype and its preparation method, belonging to the field of dairy processing technology. It includes a ternary complex of casein micelles containing AA genotype κ-casein, epigallocatechin gallate, and fructose. The preparation of the ready-to-drink milk tea includes the following steps: S1: raw material screening and pretreatment; S2: preparation of the ternary complex; S3: blending and processing of the ready-to-drink milk tea. By screening AA genotype κ-casein raw milk and using a specific sequence of first compounding EGCG and then grafting fructose, a casein micelle-EGCG-fructose ternary complex with uniform structure and controllable particle size is constructed. Fructose is further grafted onto the surface of the complex through glycosylation, effectively masking the excessive binding sites of proteins and polyphenols, significantly inhibiting the formation of insoluble aggregates, thereby maintaining excellent colloidal stability of the product during its shelf life.

The application provides a method for regulating synthesis of catechin in tea tree based on CsAHL20 gene and application, and belongs to the field of plant molecular biological technology. The tea tree gene CsAHL20 screened in the application can combine with the CsANR gene promoter of a key enzyme for synthesis of catechin, and inhibit accumulation of catechin content in tea tree; through verification, silencing of the gene by using a virus-induced gene silencing technology can increase catechin content in tea tree, and overexpression of the gene by using an agrobacterium-mediated transient transformation technology can reduce catechin content in tea tree, so that the CsAHL20 gene can be used for regulating synthesis of catechin in tea tree.

The invention belongs to the technical field of biological medicine, and particularly discloses a tea polyphenol self-polymerization nanoparticle for intracellular delivery of uricase and a preparation method and application thereof.The tea polyphenol self-polymerization nanoparticle comprises a tea polyphenol self-polymerization compound and uricase, the tea polyphenol self-polymerization compound is formed by preliminary self-polymerization of epigallocatechin gallate under catalysis of Mn < 2 + >, and the uricase is prepared from the tea polyphenol self-polymerization compound and the uricase; and mixing the polyphenol self-polymerized compound with uricase, and entrapping the uricase to obtain the tea polyphenol self-polymerized nanoparticles. The tea polyphenol self-polymerization nanoparticles have the functions of uricase combined delivery and synergistic treatment based on EGCG (epigallocatechin gallate) carriers (TPNs), the tertiary structure integrity of uricase can be effectively maintained, and the activity of uricase is ensured; meanwhile, uricase can be protected from being damaged by protease, and the stability of uricase is enhanced. Through the synergistic effect of uricase and TPNs in cells, both symptoms and root causes of intracellular urate crystallization and inflammatory reaction are treated, and a better scheme choice is provided for gout or hyperuricemia.

The application provides a tea polyphenol-copper nanomaterial and a construction method thereof; catechin compounds are dissolved in deionized water, an alkaline aqueous solution is added to adjust the pH value to 8-9, then a sodium diethyl dithiocarbamate aqueous solution and a copper salt aqueous solution are added respectively, and the tea polyphenol-copper nanomaterial is prepared. And the application in preparing a bacteriostatic agent is provided. The tea polyphenol-copper nanomaterial has the advantages of mild synthesis condition, fast reaction time, simple operation, low cost, strong bacteriostatic capacity, and the half-inhibitory concentration of MRSA and SE is less than 5 muM, and the tea polyphenol-copper nanomaterial can achieve high bacteriostatic effect at a low concentration. And the tea polyphenol-copper nanomaterial has good biological safety.

This invention provides a composition containing a naturally derived active ingredient that can suppress apoptosis of NK cells. [Solution] This is a composition for inhibiting apoptosis of natural killer cells, in which tea catechins are used as an active ingredient.

The invention discloses a low caffeine nerve stimulation tea wine based on litchi wood and sandalwood and a preparation process thereof, the wine is mainly prepared by blending litchi wood barrel aged tea wine and litchi wood / sandalwood barrel aged tea wine with different baking degrees, and the mass ratio of protocatechuic acid to catechin to epicatechin in the wine body is (0.15-0.28): 1: (0.15-0.25). The mass ratio of the protocatechuic acid to the alpha-santalol to the beta-santalol is 1: (0.4-0.6), the mass ratio of the quercetin to the gallic acid to the ferulic acid is (1.7-2.5): 1: (2.5-3.5), the mass ratio of the protocatechuic acid to the alpha-santalol is (1-2.5): 1, and the total content of the protocatechuic acid, the catechin, the epicatechin, the alpha-santalol, the beta-santalol, the quercetin, the gallic acid and the ferulic acid in the wine is 60-600mg / 100mL. By combining and matching the functional components in the wood and the proportion of the functional components, the antagonistic effect on caffeine nerve stimulation in the tea wine is achieved.

The invention relates to the technical field of taste substance marker combinations, in particular to a taste substance marker combination for identifying flavor and an application method of the taste substance marker combination. The content of quercetin, epigallocatechin, 3-p-coumaroylquinic acid (3-O-p-coumaroylquinic acid) and 4-p-coumaroylquinic acid (4-p-coumaroylquinic acid) in the areca-flavor Liupao tea is remarkably higher than that of aged aroma type Liupao tea, and the content of 3-hydroxybenzoic acid (3-hydroxybenzoic acid) in the areca-flavor Liupao tea is remarkably lower than that of the aged aroma type Liupao tea. According to the present invention, the taste substances of different aroma type tea leaf samples of the Liupao tea are analyzed by using the liquid chromatography-mass spectrometry instrument analysis method, the key taste difference compounds capable of distinguishing the aged aroma flavor and the areca-nut aroma flavor of the Liupao tea are screened out, and the taste difference of the Liupao tea can be distinguished through the discrimination model (such as principal component analysis PCA, partial least squares discriminant analysis PLS-DA and the like); and determining the flavor type according to an output judgment result.