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Method for preparing high-purity tea polyphenol in centrifuging, extracting and separating mode

A centrifugal extractor and tea polyphenol technology, applied in the field of separation, can solve the problems of low yield of active ingredients, heavy metal residues in products, high energy consumption, etc., achieve high catechin content and EGCG content, separation efficiency and production efficiency The effect of high height and small equipment footprint

Inactive Publication Date: 2012-08-08
TEA RES INST CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

The commonly used preliminary impurity removal treatment method is centrifugation. Although this method has the advantages of simple equipment and easy operation, it has problems such as low efficiency of material and liquid treatment and low yield of active ingredients.
In addition, when the above three types of methods are applied, too many organic solvents such as ethyl acetate and chloroform are often used, or Al 3+ , Ca 2+ , Fe 2+ , Mg 2+ , Zn 2+ 、Ba 2+ and other metal ions, there are problems such as difficulty in solvent recovery, heavy metal residues in the product, cumbersome and complicated process, high energy consumption, and easy environmental pollution.

Method used

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  • Method for preparing high-purity tea polyphenol in centrifuging, extracting and separating mode

Examples

Experimental program
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Effect test

Embodiment 1

[0013] Weigh 50g of tea leaves, add 500ml of deionized water (90°C) to extract for 45 minutes, take out the extract solution; then add 300ml of deionized water (90°C) to extract for 45 minutes; combine the extracts, and pump through a 300-mesh filter cloth Filtration and 0.2um ceramic membrane treatment to obtain tea polyphenol microfiltrate.

[0014] Pump the tea polyphenol microfiltrate into the first-stage centrifugal extractor, and at the same time pump an equal volume of dichloromethane solvent, turn on the centrifugal extractor at a speed of 3000rpm; then pump the extracted and separated solution into the second-stage centrifugal extractor, and simultaneously Pump in an equal volume of ethyl acetate solvent, turn on the centrifugal extractor at a speed of 3000rpm; collect and combine the ethyl acetate phase solution, concentrate in a vacuum, and spray dry to obtain high-purity tea polyphenols, in which the purity of tea polyphenols is 97.35%, and caffeine Content 0.84%. ...

Embodiment 2

[0016] Weigh 500g of tea leaves, add 5000ml of deionized water (80°C) to extract for 35 minutes, take out the extract; then add an equal volume of deionized water (80°C) to extract for 35 minutes; combine the extracts, pass through a 200-mesh filter cloth Suction filtration and 0.15um ceramic membrane treatment to obtain tea polyphenol microfiltrate.

[0017] Pump the tea polyphenol microfiltrate into the first-stage centrifugal extractor, and at the same time pump an equal volume of dichloromethane solvent, turn on the centrifugal extractor at a speed of 2500rpm; then pump the extracted and separated solution into the second-stage centrifugal extractor, and simultaneously Pump in an equal volume of ethyl acetate solvent, turn on the centrifugal extractor at a speed of 2500rpm; collect and combine the ethyl acetate phase solution, vacuum concentrate and spray dry to obtain high-purity tea polyphenols, wherein the purity of tea polyphenols is 95.67%, and caffeine Content 0.96%....

Embodiment 3

[0019] Weigh 1000g tea leaves, add 10000ml deionized water (95℃) for extraction for 45 minutes, take out the extract solution; then add 5000ml deionized water (95℃) for extraction for 45 minutes; then add 3000ml deionized water (95℃) for extraction Extract for 30 minutes; combine the extracts, filter through a 250-mesh filter cloth, and treat with a 0.10um ceramic membrane to obtain a tea polyphenol microfiltrate.

[0020] Pump the tea polyphenol microfiltrate into the first-stage centrifugal extractor, and at the same time pump an equal volume of dichloromethane solvent, turn on the centrifugal extractor at a speed of 5000rpm; then pump the extracted and separated solution into the second-stage centrifugal extractor, and simultaneously Pump in an equal volume of ethyl acetate solvent, turn on the centrifugal extractor at a speed of 5000rpm; collect and combine the ethyl acetate phase solution, concentrate in a vacuum, and spray dry to obtain high-purity tea polyphenols, in whi...

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Abstract

The invention discloses a method for preparing high-purity tea polyphenol in centrifuging, extracting and separating mode. In the existing method, excessive organic solvents or metal ions are frequently used, and problems that solvents are difficult to recover, heavy metal residues exist in products, the process is fussy and complicated, energy loss is large, environment pollution can be caused easily and the like can be caused. The method combines membrane separation and two-level centrifugal extraction for using, adopts the new process of: tea-hot water digestion- ceramic membrane microfiltration-primary centrifugal extraction-secondary centrifugal extraction-concentration-spray drying-high-purity tea polyphenol, adopts a ceramic membrane filtering method to process tea leach liquor, adopts a two-level centrifugal extractor as a key separation device, respectively performs caffeine removing, catechins purification and the like, and uses vacuum concentration extract liquor and spray drying to obtain high-purity tea polyphenol products. The method leads separation efficiency and production efficiency of tea polyphenol to be high, greatly reduces using amount of organic solvents and can obtain high-purity tea polyphenol products.

Description

technical field [0001] The invention relates to a separation method, in particular to a method for preparing high-purity tea polyphenols through centrifugal extraction and separation. Background technique [0002] Tea polyphenols are the main natural active ingredients in tea, which have various physiological activities and pharmacological effects such as anti-oxidation, anti-radiation, antibacterial and anti-inflammatory, anti-cancer, and anti-cardiovascular and cerebrovascular diseases. At present, the extraction methods of tea polyphenols mainly include the following three methods: solvent extraction, ion precipitation and column separation. The extraction of tea polyphenols is mainly based on tea extraction, and the solution after extraction usually requires preliminary impurity removal treatment. The commonly used preliminary impurity removal treatment method is centrifugation. Although this method has the advantages of simple equipment and easy operation, it has probl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/82A61P9/00A61P17/16A61P31/04A61P35/00A61P39/06
Inventor 江和源张建勇江用文王岩叶葵
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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