48 results about "Catechuic Acid" patented technology

The invention discloses a method for determining the content of catechuic acid in tea and tea products. The method comprises the following steps: preparing a gradient standard solution by adopting resorcinol instead of catechuic acid reference substance, preparing a to-be-tested solution from tea or tea products, determining the absorbance of the gradient standard solution through a hydrochloric acid-vanillin developing system under the wavelength of 510nm, establishing a standard curve taking molarity as an x-coordinate and establishing a regression equation and then determining the absorbance of the to-be-tested solution, resolving the unit concentration of resorcinol in the to-be-tested solution from the regression equation, then calculating the content of catechuic acid in tea and tea products according to the dilution factor, resorcinol unit number contained in the unit relative molecular mass of the catechuic acid reference substance, and correction factor. The resorcinol used is stable in property, easily available and low in price, the problems that the resorcinol reference substance is limited in sources, expensive in price and high in determination cost can be solved, and the method is simple to operate, accurate in results and good in repeatability.

Provided is a microorganism that is able to efficiently produce protocatechuic acid or a salt thereof by using a saccharide as a raw material, and a method of efficiently producing protocatechuic acid or a salt thereof by using the microorganism. Provided is a transformant having protocatechuic acid producing ability, subjected to modifications of enhancement of 3-dehydroshikimate dehydratase activity; enhancement of chorismate pyruvate lyase activity; and enhancement of 4-hydroxybenzoate hydroxylase activity. Also provided is a method of producing protocatechuic acid or a salt thereof, including the step of culturing the transformant in a reaction solution containing a saccharide so as to cause the transformant to produce protocatechuic acid or a salt thereof.

The invention discloses a method for converting natural anthocyanins into protocatechuic acid by using microbial whole cells to catalyze. The method uses complete cells of specific probiotics as biocatalysts, weakly acidic phosphate buffer as a medium, and natural anthocyanin extracts as a substrate to form a biotransformation anthocyanin system. The method of the invention has mild conditions, specific substrates and products, and significantly simplifies the subsequent purification process. It is a simple strategy for using microorganisms to bioconvert protocatechuic acid to anthocyanins, and no other organic matter is added during the fermentation process, which reduces the impact on the environment. possible contamination. The content of protocatechuic acid converted into the target product can reach up to 33mg / L. The invention is of great significance for in vitro research on the mechanism of microbial metabolic conversion of anthocyanin, and also provides a theoretical basis for the direct application of natural anthocyanin conversion products to probiotic fermented food.

The invention discloses a method for improving the color and texture quality of vacuum freeze-dried recombined strawberry chips, which comprises the following steps: step 1, crushing strawberries to obtain strawberry pulp, mixing the strawberry pulp with pectin and protocatechuic acid evenly, And adjust pH to 3.0~3.8 to obtain material A; Wherein, the mass ratio of strawberry pulp and pectin is 1:0.02~0.08, the mass concentration of protocatechuic acid in material A is 0.1~1.0g / kg; Step 2, the material A is poured into a mold tray, placed in a freezer at -18°C for freeze-thaw processing to obtain block material B; step 3, coating a layer of chitosan on the surface of material B to obtain material C; step 4, placing material C in Dry in a vacuum freeze-drying box, and then pack with nitrogen filling to obtain strawberry crisps. The invention creates a brand-new recombined strawberry crisp, which effectively solves the problems of unstable storage period of the product geranium-3-glucoside and low hardness and brittleness of the product.

This invention comprises applying phytoceutical compositions to pets, which given their sedentary life and nutrition, are susceptible to a higher prevalence of cancer and degenerative diseases. The compounds contained in said compositions frequently have anti-oxidizing properties acting to inhibit harmful reactions of free radicals; and free radicals are often a cause of cancer. Said compositions comprise extracts or concentrates obtained from the enzymatic or alkaline hydrolysis of several plant sources, containing effective concentrations of two or more phenolic compounds, such as ferulic acid, coumaric acid, caffeic acid, protocathecoic acid, and vanillic acid.

The invention discloses a method for simultaneously determining twenty-two flavones and phenolic acids in citrus fruits by adopting high performance liquid chromatography-diode array detector-fluorescence detector (HPLC-DAD-FLD). The method is capable of simultaneously determining twenty-two phenolic compounds in citrus fruits such as gallic acid, synephrine, chlorogenic acid, protocatechuic acid,caffeic acid, p-coumaric acid, rhamnosylvitexin, eriocitrin, ferulic acid, rutin, benzoic acid, narirutin, naringin, hesperidin, diosmin, neohesperidin, quercetin, naringenin, kaempferol, nobiletin,hesperetin, acacetin and the like, derivatization is not needed, and the method is high in accuracy, high in sensitivity and excellent in repeatability.