26 results about "Catechuic Acid" patented technology

The application belongs to the field of enzyme engineering and biotechnology, and discloses a 4-hydroxybenzoic acid hydroxylase mutant and application thereof in synthesis of gallic acid or a salt thereof. The 4-hydroxybenzoic acid hydroxylase mutant is obtained through directional evolution modification and high-throughput screening, and has an increased protocatechuate hydroxylase activity by more than 4 times. The 4-hydroxybenzoic acid hydroxylase mutant is expressed by using E. coli, and the obtained recombinant E. coli can efficiently catalyze the conversion of protocatechuate into gallic acid. The 4-hydroxybenzoic acid hydroxylase mutant is expressed in the recombinant E. coli for producing protocatechuate, and the obtained recombinant E. coli can de novo synthesize gallic acid from glucose as a substrate, and the yield reaches 58.65 g / L, and the residual amount of protocatechuate is only 0.33 g / L. The application solves the problem of a large amount of residual protocatechuate in the fermentation process of the existing strains, and can provide an efficient enzyme element for the creation of gallic acid industrial strains.

The application discloses an aluminum profile spraying powder coating and a preparation method thereof, and belongs to the technical field of paint. The application introduces modified resin, so that the coating has excellent weather resistance, and adds specific proportion of proto-catechuic acid, 2-(4-thiazolyl) benzimidazole and 8-hydroxyquinoline as an antibacterial agent, and chemically bonds the antibacterial agent to the resin base material, so that efficient, long-lasting and broad-spectrum antibacterial effect is realized, and more significant antibacterial purpose is realized, and the problems of easy migration and easy failure of the surface spraying antibacterial agent are effectively solved. The application optimizes the overall formula, and the components interact with each other, so that the coating has excellent surface flatness and apparent quality, and the defects of pinholes and orange peel commonly existing in traditional coatings are avoided; the overall antibacterial and long-lasting antibacterial properties are significant, and the application is particularly suitable for buildings, medical treatment and household aluminum profiles with high sanitary requirements.

The invention relates to the technical field of liqueur, in particular to liqueur and a preparation method thereof.The liqueur is prepared by soaking various medicinal and edible raw materials such as kudzuvine roots, rice, barbary wolfberry fruits, smoked plums, grapes, mulberries, poria cocos, Chinese yams, rhizoma polygonati, jujubes and ginseng in baijiu base liquor according to a specific proportion. According to the present invention, the modern analysis technology shows that the wine contains 25 active components such as resveratrol, protocatechuic acid, oleanolic acid, quercetin, kaempferol and the like, and the components achieve the synergistic effect, such that the product has effects of tumor resistance, liver protection, blood sugar reduction, blood fat reduction, oxidation resistance, inflammation resistance, nerve protection and other health-care functions, and the product has characteristics of definite component, synergistic effect, high safety, and no toxic-side effect. The preparation process is stable and controllable, and is suitable for large-scale production.

The invention provides a medicament for treating a milk disease of eriocheir sinensis and a preparation method of the medicament. The medicament is prepared from the following raw materials in parts by weight: 6-7 parts of traditional Chinese medicine components, 2 parts of allicin and 1 part of an additive, wherein the traditional Chinese medicine components comprise coptis chinensis, golden cypress, schisandra chinensis, astragalus membranaceus and gallnut, and the additives comprise epsilon-polylysine, protocatechuic acid and rosmarinol. According to the medicament for treating the milk disease of the eriocheir sinensis, the efficacy limitation of a single natural medicine is broken through through a technology of combining the compound traditional Chinese medicine components with the allicin and the additive, the application of the natural medicine of aquatic products is promoted, a culture mode is assisted to be transformed to be green and efficient, and a brand new thought is provided for treating the milk disease.

The present invention belongs to the field of traditional Chinese medicine detection technology, and specifically relates to a method for constructing a characteristic spectrum of a tsaoko kernel and its preparation and a method for distinguishing the same from counterfeit products, comprising the following steps: (1) preparing a test solution; (2) taking the test solution and detecting it by high performance liquid chromatography, with a wavelength of 238-242 nm, octadecylsilane bonded silica gel as a filler, a mobile phase comprising an aqueous solution containing phosphoric acid and acetonitrile, and a gradient elution program comprising: 0 → 20 min → 30 min → 50 min, and the volume percentage of acetonitrile in the mobile phase being: 4% → 19% → 34% → 45%. The characteristic spectrum obtained by the method has a stable baseline, good characteristic peak shape, high separation, and can accurately locate the peak positions of protocatechuic acid, proanthocyanidin B2, and citral (neral, geranial), fully reflecting the integrity and characteristic properties of the tsaoko kernel and its preparation, and providing a basis for quality detection and control of the tsaoko kernel and its preparation.

The application provides a cool polyamide fiber and a preparation method thereof, and relates to the technical field of fiber materials. The preparation method of the cool polyamide fiber is as follows: 96-100 parts of polyamide 56 chips, 2-3 parts of surface-treated jade powder and 5-6 parts of a phosphine sapphyrin silicon-containing flame retardant are mixed at high speed, extruded and granulated to prepare polyamide master batches; the polyamide master batches are melt-spun to prepare polyamide fibers; the polyamide fibers are sequentially finished with an aqueous formaldehyde solution and proto-catechuic acid, and then washed, dried and cooled to prepare the cool polyamide fiber. The cool polyamide fiber provided in the application has the functions of coolness, bacteriostasis and flame retardation.

The invention belongs to the technical field of enzyme engineering and biology, and discloses a 4-hydroxybenzoic acid hydroxylase mutant and application thereof in synthesis of gallic acid or gallic acid salt. The 4-hydroxybenzoic acid hydroxylase mutant is obtained through directed evolution modification and high-throughput screening, and the protocatechuic acid hydroxylase activity of the 4-hydroxybenzoic acid hydroxylase mutant is improved by more than 4 times; escherichia coli is utilized to express the 4-hydroxybenzoic acid hydroxylase mutant, and the obtained recombinant Escherichia coli can efficiently catalyze and convert protocatechuic acid into gallic acid; the 4-hydroxybenzoic acid hydroxylase mutant is expressed in recombinant escherichia coli for producing protocatechuic acid, the obtained recombinant escherichia coli can be used for synthesizing gallic acid from the beginning by taking glucose as a substrate, the yield reaches 58.65 g / L, and the residual amount of protocatechuic acid is only 0.33 g / L. The problem that a large amount of by-product protocatechuic acid is left in the fermentation process of existing strains is solved, and an efficient enzyme element can be provided for creation of gallic acid industrial strains.

The application discloses application of protocatechuic acid ethyl ester as a feed additive to improving egg laying rate of laying hens. The protocatechuic acid ethyl ester is added into feed of the laying hens as the feed additive, so that the anti-aging ability and production performance of the aging laying hens can be improved, and the feed additive is simple to feed, and can be uniformly mixed in the daily ration and fed, and the storage method of the feed additive is simple, and the feed additive can be well stored at normal temperature, and the feed additive does not lose biological activity, has no toxic side effect, has no residual harm, and is safe and effective.

This invention discloses a genetically engineered bacterium for synthesizing protocatechuic acid and its applications, belonging to the field of gene recombination technology. The genetically engineered bacterium for synthesizing protocatechuic acid disclosed in this invention is a recombinant *Corynebacterium glutamicum* strain based on *Corynebacterium glutamicum* ATCC13032, or a *Corynebacterium glutamicum* strain modified with a chassis microbial substrate, including the shikimic acid pathway; and / or the DAHP pathway; and / or the 4-HBA pathway. The highest concentration of protocatechuic acid obtained by fermentation of the recombinant *Corynebacterium glutamicum* constructed in this invention can reach 85.16 g / L, which is the highest yield of protocatechuic acid synthesized de novo by biological methods reported in related studies to date. This has great potential for the industrial application of high-yield protocatechuic acid by biological methods. The genetically engineered bacterium constructed in this invention is safe and non-toxic, and can efficiently produce protocatechuic acid using glucose as a substrate through microbial fermentation. Furthermore, the culture medium composition is simple, batches are stable, and production costs are low.

The invention relates to an asymmetric adhesion bionic hydrogel dressing and a preparation method thereof. The preparation method is characterized by comprising the following steps: (1) dissolving acrylamide and acrylic acid in water; then adding N, N '-methylene bisacrylamide and N, N, N', N '-tetramethylethylenediamine, then adding FeCl3 and ammonium persulfate, and carrying out crosslinking to obtain upper-layer hydrogel; (2) adding protocatechuic acid, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide into the hydroxypropyl chitosan solution, so as to obtain an adhesive layer; and (3) coating one surface of the upper-layer hydrogel with the adhesion layer, and curing at room temperature to obtain the asymmetric adhesion bionic hydrogel dressing. The asymmetric adhesion bionic hydrogel dressing has the advantages that one surface of the prepared asymmetric adhesion bionic hydrogel dressing has good adhesion performance and is used for being adhered to a wound, and the other surface of the asymmetric adhesion bionic hydrogel dressing is low in adhesion performance; the adhesive composition can be firmly adhered to tissues and can prevent the generation of bad adhesion.

The invention provides a recombinant saccharomyces cerevisiae strain for improving the yield of aromatic acid as well as a construction method and application of the recombinant saccharomyces cerevisiae strain. A saccharomyces cerevisiae strain PC01 for synthesizing protocatechuic acid and p-coumaric acid is used as a chassis, fbp1, pfk1, pfk2 and shb17 are knocked out, and an endogenous escape path and invalid cycle of saccharomyces cerevisiae are blocked. Meanwhile, a p-coumaric acid synthesis pathway is knocked out, so that the inhibition of p-coumaric acid on the growth of the recombinant saccharomyces cerevisiae is relieved. Then, overexpressing a protocatechuic acid synthetase gene so as to enhance the metabolic flux of the protocatechuic acid, and overexpressing Aro3D154N, Aro4K229L, EcAroB and EcAroD so as to enhance the synthesis of the main carbon flow to the protocatechuic acid. And finally, integrating other aromatic acid metabolic pathways to realize biosynthesis of salicylic acid, p-hydroxybenzoic acid and gallic acid. And the universality of the engineering strain is verified. The recombinant strain provided by the invention takes glucose as a carbon source, so that not only is the production cost reduced, but also the stable hereditary character can be maintained, and the high yield of aromatic acid is ensured.

The invention provides recombinant escherichia coli for synthesizing vanillyl alcohol as well as a construction method and application of the recombinant escherichia coli. Wherein the recombinant escherichia coli comprises a host bacterium, and the host bacterium overexpresses a DAHP synthetase encoding gene AroGfbr and a 3-dehydroshikimic acid dehydratase encoding gene QuiC on an escherichia coli genome by using a pTrc promoter, so that sufficient supply of protocatechuic acid is realized; an alcohol dehydrogenase encoding gene ADH4 or ADH6 is introduced, so that the conversion speed of a toxic intermediate 3, 4-dihydroxybenzaldehyde is accelerated, and the fermentation density of the strain is improved; genes mtn and luxs of a methylation cofactor SAM regeneration pathway are over-expressed on a genome, so that the methylation efficiency is improved. Therefore, the recombinant Escherichia coli provided by the invention can efficiently synthesize vanillyl alcohol through the combination of the transformation strategies, and can efficiently accumulate 1.66 g / L vanillyl alcohol in a shake flask, and the yield of a 3L fermentation tank reaches 6.21 g / L. The method for producing vanillyl alcohol by using the recombinant strain provided by the invention has the advantages of low cost, controllable process, simplicity and convenience in separation and extraction, and facilitation of industrial production.

The present invention discloses a method for establishing a UPLC fingerprint of Weimaining capsules, comprising the following steps: (1) preparing a test solution; (2) respectively examining the precision of an ultra-high performance liquid chromatograph, the repeatability of a test solution preparation method, and the stability of the test solution; (3) subjecting the test solution to gradient elution in a chromatographic column, and scanning the test solution using an ultra-high performance liquid chromatograph to obtain a chromatogram of the test solution; (4) identifying the common peak components of the chromatogram using reference substances such as gallic acid, protocatechuic acid, protocatechuic aldehyde, proanthocyanidin B2, epicatechin, and rutin. (5) establishing a fingerprint. The ultra-high performance liquid chromatograph used in the present invention has high precision, and the test solution preparation method provided has good repeatability. The fingerprint established based on the above two points has strong stability, and the speed of establishing the fingerprint is fast, the sensitivity is high, and the separation of the chromatographic peaks is good.

The present invention discloses a method for constructing a characteristic spectrum of golden buckwheat. The characteristic spectrum has gallic acid, protocatechuic acid, protocatechuic aldehyde, catechin, epicatechin, 4-coumaric acid, and procyanidin B2 as characteristic components. By using an efficient detection method, the common peaks of golden buckwheat samples of different origins are confirmed to construct a characteristic spectrum. The method can distinguish the counterfeit products of golden buckwheat, Smilax glabra and Smilax china, and with the flavonoids contained in golden buckwheat as representative components, it can more comprehensively reflect the product quality. The method is suitable for rapid and comprehensive quality control of golden buckwheat medicinal materials, decoction pieces, extracts, standard decoctions, formula granules, and benchmark samples, preparation intermediates, and finished products of golden buckwheat classic prescriptions. The method is efficient, easy to operate, and suitable for quality monitoring of continuous production.

The invention relates to the technical field of traditional Chinese medicine detection, and particularly provides a construction method and a content determination method of specific chromatograms of a Guangxi kadsura pepper stem aqueous extract and a preparation thereof, and the method comprises the following steps: (1) preparing a reference substance solution; (2) preparing a test solution; (3) detecting by adopting an ultra-high performance liquid chromatography to obtain seven or more common characteristic peaks, and realizing effective separation of the characteristic peaks containing gallic acid, protocatechuic acid, gallocatechin, protocatechualdehyde, catechin and epicatechin, so that the peak shape is good, and the base line is stable; and (4) taking catechin as an internal reference substance, establishing relative correction factors of protocatechuic acid, epicatechin and catechin, and establishing a content determination method of quantitative analysis of multi-components by single marker of three components. According to the method constructed by the invention, the content of the characteristic chromatogram and the content of the characteristic components can be simultaneously detected, the measured components can be completely separated, the detection time is short, and a basis is provided for quality detection and control of the Guangxi kadsura pepper stem aqueous extract and preparations thereof.

The invention relates to the technical field of food preservation, and particularly discloses a screening method and application of plant lactobacillus Q9 with an agaricus bisporus postharvest preservation function. And a screening step: separating suspected lactic acid bacteria from traditional fermented milk in Gannan in Qinghai-Tibet Plateau, carrying out Gram-positive and catalase-free activity identification, screening strains which grow well and are high in polyphenol conversion rate by taking agaricus bisporus characteristic polyphenols such as gallic acid and protocatechuic acid as substrates, and carrying out sensory evaluation to screen out the plant lactobacillus Q9. When the Q9 bacterial suspension is applied, the bacterial suspension containing 1.5 * 10 < 8 > CFU / mL of Q9 is sprayed on the surface of agaricus bisporus, browning can be inhibited, the weight loss rate and the respiration rate can be reduced, the growth of pseudomonas can be inhibited, the related enzyme activity can be regulated and controlled, the shelf life can be prolonged, and a safe and efficient biological fresh-keeping scheme is provided for the postharvest fresh-keeping of agaricus bisporus.

The invention discloses an aluminum profile spraying powder coating and a preparation method thereof, and belongs to the technical field of coatings. The modified resin is introduced, so that the coating has excellent weather resistance, protocatechuic acid, 2-(4-thiazolyl) benzimidazole and 8-hydroxyquinoline in a specific proportion are added and compounded to serve as an antibacterial agent, the antibacterial agent is chemically bonded into a resin base material, the efficient, lasting and broad-spectrum antibacterial effect is achieved, the more remarkable antibacterial purpose is achieved, and the coating has the good application prospect. The problems that an antibacterial agent sprayed on the surface is prone to migration and failure are effectively solved. According to the invention, the whole formula is optimized, and the components interact with one another, so that the coating has excellent surface flatness and apparent quality, and the common defects of pinholes and orange peels in the traditional coating are avoided; the antibacterial aluminum profile has remarkable antibacterial property and lasting antibacterial property on the whole, and is particularly suitable for buildings, medical treatment and home furnishing aluminum profiles with high sanitary requirements.

The invention relates to the technical field of food additives and controlled release, in particular to a sodium chloride controlled-release microcapsule with a three-layer structure and a preparation method of the sodium chloride controlled-release microcapsule. The microcapsule comprises: a core layer: a sodium chloride crystal; the inner-layer composite wall is prepared from mung bean protein, chitosan and polyacrylic acid; the middle layer composite wall is a protocatechuic acid layer; the outer-layer composite wall is prepared from sodium alginate, calcium ions and protocatechuic acid gel. The preparation method of the sodium chloride controlled-release microcapsule with the three-layer structure comprises the following steps: S1, preparing a solution; s2, preparing a ternary composite wall material solution; s3, coating the sodium chloride core with the ternary composite wall material; s4, carrying out crosslinking treatment on protocatechuic acid; s5, spray drying; and S6, carrying out protocatechuic acid cross-linking treatment for the second time and coating an outer layer. A unique three-layer structural design is adopted, the dual goals of high stability in a non-oral environment and accurate response release in an oral environment are achieved, and the functionality and safety of the product are improved.

The present invention discloses a method for constructing a characteristic spectrum of a butterfly fruit medicinal material, comprising: preparing a reference solution, wherein the reference solution comprises gallic acid, protocatechuic acid, dihydromyricetin, p-hydroxycinnamic acid, vinazenin-II, and myricetin; taking the butterfly fruit medicinal material to prepare a test solution; performing high-performance liquid chromatography analysis on the reference solution and the test solution to obtain a reference chromatogram and a test sample chromatogram, and establishing a characteristic spectrum; wherein the conditions of the high-performance liquid chromatography analysis include: using an octadecylsilane bonded silica gel chromatographic column, acetonitrile as mobile phase A, and formic acid aqueous solution as mobile phase B. The present invention establishes an HPLC characteristic spectrum of the butterfly fruit, combines similarity evaluation of the characteristic spectrum with chemometric analysis to analyze the quality of butterfly fruit medicinal materials from different origins, screens out differential markers, and determines their content.

The invention relates to the technical field of antibacterial dressings, and discloses an antibacterial dressing based on tricholoma mongolicum polysaccharide and a preparation method thereof.The antibacterial dressing based on the tricholoma mongolicum polysaccharide is prepared from sodium alginate, hyaluronic acid, glycerin, tricholoma mongolicum polysaccharide sustained-release microcapsules and deionized water; wherein the tricholoma mongolicum polysaccharide sustained-release micro-capsule is an inclusion compound formed by taking mussel mucin and protocatechuic acid modified cellulose as wall materials and tricholoma mongolicum polysaccharide as a core material; the protocatechuic acid modified cellulose is prepared by reacting protocatechuic acid with sodium carboxymethyl cellulose; the tricholoma mongolicum polysaccharide is prepared through multi-enzyme auxiliary extraction, the antibacterial dressing prepared through the method has an excellent and lasting antibacterial effect, and the healing process of skin wounds can be remarkably accelerated.

This invention discloses an HPLC method for the simultaneous determination of five components—protocatechuic acid, verbascoside, argentin, verbascoside, and argentin—in Compound Euonymus fortunei Capsules. The chromatographic conditions used are as follows: an Acclaim 120-C18 column (250 mm × 4.6 mm, 5 μm) is used, with acetonitrile-0.2% phosphoric acid solution as the mobile phase, gradient elution, and a flow rate of 1 ml / min. ‑1 The column temperature was 30℃, and the detection wavelength was 254nm. Under these chromatographic conditions, the linear ranges for the five components were: protocatechuic acid Y = 11726348.35x + 87150.17; (R 2 =0.9999), verbascoside Y = 27134X - 0.5781; (R 2 =0.9994), stigmoside Y = 29605X + 14.0030; (R 2 =0.9996), verbascoside Y = 51970X - 25.7380; (R 2 =0.9991), styracin Y = 61726X + 7.1802; (R 2 =0.9991). The chromatographic peaks of protocatechuic acid, verbascoside, gentianin, verbascoside, and gentianin were well separated, and the concentration and peak area showed a good linear relationship. This method is simple, rapid, accurate, and reproducible, and can provide a theoretical basis for the quality of Compound Euonymus fortunei Capsules.

The application provides an Escherichia coli (E. coli) recombinant strain for producing 2-pyrone-4,6-dicarboxylic acid. Escherichia coli The recombinant strain is obtained by overexpressing an exogenous 3-dehydroshikimic acid dehydrase gene on the genome of a 3-dehydroshikimic acid-producing recombinant strain, or further overexpressing a protocatechuate-4,5-dioxygenase gene, or further overexpressing a 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase gene, so as to realize high-efficiency de novo synthesis of 2-pyrone-4,6-dicarboxylic acid. The strain utilizes an inorganic salt culture medium, takes glucose as a carbon source, and produces 2-pyrone-4,6-dicarboxylic acid through batch feeding fermentation, so that the production cost is low, and the above-mentioned recombinant strain does not contain a plasmid and has high genetic stability.

The present application provides a kind of content determination method of Fuyanxiao preparation, belong to the technical field of drug detection, the Fuyanxiao preparation is made of seven medicinal materials such as Oxalis corniculata L., Herba Patriniae, Radix Trichosanthis, Radix et Rhizoma Rhei, Cortex Moutan, Atractylodes, Linder, etc., the content determination method is using high performance liquid chromatography method same chromatographic condition is carried out content determination to 14 kinds of components in product, such as gallic acid, protocatechuic acid, epicatechin, aloe-emodin glycoside, isorhamnetin, swertisin, emodin glycoside, chrysophanol glycoside, physcion glycoside, aloe-emodin, rhein, emodin, chrysophanol, physcion.The present application increases the content determination of gallic acid, protocatechuic acid, epicatechin, aloe-emodin glycoside, isorhamnetin, swertisin, emodin glycoside, chrysophanol glycoside, physcion glycoside, aloe-emodin, rhein, chrysophanol, physcion compared with prior art, improves the requirement of product quality detection, is favorable for guaranteeing drug quality and clinical efficacy.

A method for collecting blood and preparing serum from giant freshwater prawns includes: feeding the prawns a nutrient-rich diet under low-temperature conditions, collecting blood from their second walking legs, and obtaining purified serum through blood coagulation, serum precipitation, and further processing; the nutrient diet includes at least a protocatechuic acid-chitosan oligosaccharide derivative, which is obtained by grafting protocatechuic acid onto chitosan oligosaccharide. This invention preserves the life of individual giant freshwater prawns during blood collection and increases the amount of blood and serum collected.

The invention relates to the technical field of screening of pharmacodynamic substances of natural products, and particularly discloses a method for screening a hypoglycemic core pharmacodynamic substance from brown coal source fulvic acid. The method integrates a double-layer progressive screening strategy and a double verification mechanism, and comprises the following steps: firstly, carrying out double screening through chemical analysis and network pharmacology in combination with topology parameters and literature evidences so as to lock a core pharmacodynamic compound; performing quantitative screening based on the target interaction network to determine a core target; finally, verification is completed through molecular docking and in-vitro alpha-glucosidase inhibition experiments. According to the method, nine core pharmacodynamic compounds such as gallic acid and protocatechuic acid are successfully screened, and a scientific basis and a technical path are provided for clarification of a multi-target synergistic effect mechanism and development of hypoglycemic functional products.

The application discloses a kind of benzoxazine resin based on protocatechuic acid and synthesis and application thereof, with protocatechuic acid as raw material, first with furfurylamine to carry out amidation, then with furfurylamine and paraformaldehyde one-pot synthesis protocatechuic acid-based benzoxazine monomer (NDBA-fa);The resin monomer can be cured to form protocatechuic acid benzoxazine resin alone.Use NDBA-fa to enhance the mass content of bisphenol A-aniline benzoxazine (BA-a) is 7.5%, and the glass transition temperature of the cured resin T g , thermal decomposition temperature T d5% 、T d10% , carbon residue rate is increased by 21 ℃, 14 ℃, 23 ℃, 16.8%, and the heat release capacity HRC is decreased by 76.3 Jg ‑1 K ‑1 The protocatechuic acid-based benzoxazine monomer provided by the application can be used as a new modifier, which can effectively improve the thermal stability and flame retardant property of BA-a. The application range of biological resources is also widened.