Alcaligenes family aerobic new bacterium T-4 and application thereof

A technology of alcaligenes and aerobic bacteria, applied in the direction of aerobic process treatment, bacteria, water/sludge/sewage treatment, etc., can solve the problem of few reports of small molecule degradation products, and achieve the effect of wide application prospects

Active Publication Date: 2021-11-16
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is generally believed that biodegradable plastics are converted into carbon dioxide, microbial biomass and water by microorganisms under composting conditions. CO 2 That is, if the concentration of the remaining organic components is lower than 1%, there is no need to consider it. In addition, the polyesters reported in the literature can be degraded by microorganisms in the environment, mainly by degrading the ester bond of the lipase produced by the bacteria to hydrolyze the polymer to make it Degraded into small molecular oligomers or monomers, but there are few reports on whether these bacteria can continue to degrade or metabolize small molecular degradation products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Alcaligenes family aerobic new bacterium T-4 and application thereof
  • Alcaligenes family aerobic new bacterium T-4 and application thereof
  • Alcaligenes family aerobic new bacterium T-4 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Enrichment of Compost Samples and Isolation, Purification and Preservation of Terephthalic Acid Degrading Bacteria T-4

[0029] Take 2g of the compost sample and add it to 100mL enrichment medium MMTA, shake and culture at 50°C and 180rpm for one week, detect the TPA concentration before and after the culture of the bacteria solution, if TPA is degraded, dilute the culture solution in a gradient, and spread it on the LBTA medium solid Plates were incubated at 50°C. Pick a single colony, streak it on a new LBTA medium, and further isolate and purify to obtain a single strain. The obtained single strain was re-screened with the enrichment medium, and the strain determined to degrade terephthalic acid was stored in a glycerol tube with a final concentration of 20% glycerol, and stored in a -80°C refrigerator.

Embodiment 2

[0030] Embodiment 2: the strain identification of bacterial strain T-4

[0031] (1) Morphological characteristics of bacterial colonies

[0032] After the strain T-4 was cultured in LBTA solid medium at 50°C for 3 days, the colony was off-white, round, convex and smooth, with neat edges, see figure 1 .

[0033] (2) Morphological characteristics of the bacteria

[0034] The transmission electron microscope image of the strain T-4 cultured in the improved MMTA liquid medium at 50°C and 180rpm for 18 hours. The cells are spherical or slightly ellipsoidal in shape, with a diameter of 1.08-1.4 μm. They have flagella, and the flagella are multiple and implanted in different positions. fixed, see figure 2 , where A-F represent different cells, and the magnifications of A, B, C-E, and F are 1.5k, 2.0k, 3.0k, and 4.0k, respectively.

[0035] (3) Taxonomic status of strain T-4

[0036] The bacterial genomic DNA extraction kit (Axgen) was used to extract the total DNA of strain T-4...

Embodiment 3

[0042] Example 3: Degradation of TPA by bacterial strain T-4

[0043] (1) Growth curve of strain T-4 degrading TPA

[0044] Bacterial strain T-4 was cultivated in the improved MMTA liquid medium, the filling volume of 250mL shake flask was 100mL, 1% inoculum size, 50°C and 180rpm shaking culture, and samples were taken at different time points to detect the bacterial concentration (OD 600 ), TPA concentration and pH. The concentration of terephthalic acid in each group was determined by ultraviolet-visible spectrophotometry: after the samples were centrifuged at 10,000 rcf for 2 minutes, the optical density OD of the supernatant of the bacterial liquid was detected 240 , it is necessary to make a standard curve with MMTA solutions of different terephthalic acid concentrations.

[0045] The growth curve of strain T-4 degrading TPA is as follows Figure 4 As shown, the strain T-4 grows gradually with the degradation of terephthalic acid, and the pH will increase during the de...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an aerobiotic bacterium T-4 of alcaligenes family and application thereof. The alcaligenes aerobic bacterium T-4 is preserved in CGMCC, the preservation date is September 11, 2019, and the preservation number is CGMCC No.18488. According to the invention, a single strain T-4 is adopted for aerobic culture at 50 DEG C to effectively degrade terephthalic acid, and the degradation rate reaches 100% when the initial concentration does not exceed 50mM. The strain can be used for treating terephthalic acid wastewater, can also be used for completely degrading biodegradable plastics containing terephthalic acid groups in compost or soil environment, and can also be used for other important phenyl compounds, e.g. degradation of m-phthalic acid, protocatechuic acid, benzoic acid, m-hydroxybenzoic acid, p-hydroxybenzoic acid, 4-formylbenzoic acid, bisphenol A and 4,4-diaminodiphenylmethane. The strain does not cause secondary pollution, and has wide application prospects in the environmental restoration aspect.

Description

technical field [0001] The invention belongs to the field of microorganisms and their biodegradation applications, and relates to a new aerobic bacterium T-4 of the family Alcaligenes and its application, in particular to the isolation and identification of a new aerobic bacterium T-4 of the family Alcaligenes, and its application Degradation of terephthalic acid, phthalic acid, isophthalic acid, protocatechuic acid, benzoic acid, m-hydroxybenzoic acid, p-hydroxybenzoic acid, 4-formylbenzoic acid, bisphenol A, 4 in environmental remediation , One or more applications of 4-diaminodiphenylmethane. Background technique [0002] Terephthalic acid (TPA), as an important raw material in the petrochemical industry, is mainly used in the manufacture of biodegradable polyesters, such as polyethylene terephthalate (PET), polybutyl adipate-terephthalic acid Butyl ester (PBAT) is also widely used in the production of plasticizers, polyester fibers, dyes, spices, pesticides and other co...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C02F3/02C02F3/34B09C1/10C12R1/01C02F101/38C02F101/34
CPCC02F3/02C02F3/34B09C1/10C02F2101/38C02F2101/34C02F2101/345Y02W10/10
Inventor 刘倩倩高俊飞顾群
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap