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Method for preparing high-purity monomer epigallocatechin gallate from processed leftovers of tea leaves

A technology for leftovers and catechins, applied in the direction of organic chemistry, etc., can solve the problems of poor separation effect, inability to form a process, ideal yield, etc., and achieve the effects of high product purity, less equipment, and elimination of chloroform residues

Inactive Publication Date: 2012-10-03
GUANGXI JIKANG BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0023] The above methods all have shortcomings, some have ideal yield, some have poor separation effect, some have high cost, and some need to use special materials
In addition, these studies generally only focus on distinctive innovations, but cannot form a complete set of processes.

Method used

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  • Method for preparing high-purity monomer epigallocatechin gallate from processed leftovers of tea leaves
  • Method for preparing high-purity monomer epigallocatechin gallate from processed leftovers of tea leaves
  • Method for preparing high-purity monomer epigallocatechin gallate from processed leftovers of tea leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Dry tea leftovers (green tea, not black tea), 100g, crushed into 50 mesh, add 2000ml of water, warm to 60℃ soak, adjust PH=6, add a mixed enzyme composed of cellulase, protease and pectinase (Ratio 1:1:1), the compound enzyme dosage is 0.5% of the dry weight of the tea, stirred for 60 min, filtered, and repeated extraction 3 times. The combined filtrate was extracted with ethyl acetate and then concentrated and dried in vacuo. 21.8 g of tea polyphenols with a content of 18.1% were obtained. According to the dry tea containing 30% of tea polyphenols and 22% of catechins, the extraction rate of tea polyphenols is 13.2%. The content of catechins is 8.1%, and the extraction rate is 8%.

Embodiment 2

[0081] Dry fresh tea leaves at 60°C, take 100g of dry weight, smash into 20 meshes, add 1000ml of water, warm to 40°C and soak, adjust PH=3, add mixed enzyme composed of cellulase, protease and pectinase (proportion 5:0.1:5), the dosage of the complex enzyme is 0.1% of the dry weight of the tea, stir for 90min, filter, and repeat the extraction 3 times. The combined filtrate is extracted with ethyl acetate and then concentrated and dried in vacuo. 34.6 g of tea polyphenols with a content of 17.5% were obtained. According to the dry tea containing 36% of tea polyphenols and 28% of catechins, the extraction rate of tea polyphenols is 16.8%. The content of catechin is 7.6%, and the extraction rate is 9.4%.

Embodiment 3

[0083] 100g of dry tea leaves the same as in Example 1, crushed into 80 meshes, added 1500ml of water, heated to 50°C and soaked, adjusted PH=6.5, and added a mixed enzyme composed of cellulase, protease and pectinase (ratio 0.1: 5:5) The dosage of the compound enzyme is 1% of the dry weight of the tea, stirring for 30min, filtering, and repeating the extraction 3 times. The combined filtrate is extracted with ethyl acetate and then concentrated and dried in vacuo. 26.5 g of tea polyphenols with a content of 16.9% were obtained, and the extraction rate of tea polyphenols was 14.9%. The content of catechin is 6.97%, and the extraction rate is 8.4%.

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Abstract

The invention relates to a method for preparing high-purity monomer epigallocatechin gallate from processed leftovers of tea leaves. The method comprises the following steps of: extracting tea polyphenol, namely performing enzymolysis of the processed leftovers, which serve as raw materials, of fresh tea leaves, pruned tea leaves and non-fermented tea leaves by adding complex enzyme which consists of cellulose, protease and pectinase to extract the tea polyphenol; and separating a catechin out for purifying, namely precipitating the tea polyphenol by using a mixed precipitant which consists of CaCl2 and ZnCl2, performing primary separation by a macroporous resin chromatographic column, and performing secondary purification by the macroporous resin chromatographic column to obtain the high-purity monomer epigallocatechin gallate (EGCG). According to the method, chloroform is not used, the join from the extraction process to the refining and purification processes is compact, and the quality and yield of the product are high.

Description

Technical field [0001] The present invention relates to plant extracts, especially tea extracts, in particular to a method for preparing high-purity monomer catechins from leftovers of tea processing. Background technique [0002] As a traditional health beverage of the Chinese nation, tea has a history of four to five thousand years. my country is a big country in the production of tea with abundant tea resources, with an annual output of about 600,000 tons of finished tea. [0003] The development of new uses of tea and the comprehensive utilization of tea, especially the production of high-value-added fine chemicals using low-grade tea or tea processing leftovers, is an important research topic. In the early 1960s, Japanese scientists discovered that tea extract contained an antioxidant active ingredient. Scientists from various countries have conducted in-depth studies and proved that it is a class of polyphenol compounds, namely tea polyphenols (TP). Tea polyphenols is a gen...

Claims

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Application Information

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IPC IPC(8): C07D311/62C07G99/00
Inventor 李健王家祥易建平
Owner GUANGXI JIKANG BIO TECH
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