74 results about "Agmatine" patented technology

The invention relates to the use of agmatine, in the treatment of acute neurotrauma (such as stroke) and degenerative disorders of the central and peripheral nervous system (such as dementia). The invention further provides novel compounds of general formula I (which are quinuclidine derivatives), formula II (which are norbornane derivatives), formula III (which are adamantane derivatives), and formula IV (which are phenothiazine derivatives): wherein R1, R2 and R3 are each independently hydrogen, hydroxy, substituted or unsubstituted C1-4 alkyl, substituted or unsubstituted C1-4 alkoxy, halogeno, amino, phenyl, or R4NR5; R4 and R5 are each independently hydrogen, or (CH2)n-[NH(CH2)x]y-NHR6, or (CH2)n-[NH(CH2)x]y-NH-NHR6, or (CH2)n-[NH(CH2)x]y-(NR7=)CNHR6, or (CH2)n-[NH(CH2)x]y-NH(NR7=)CNHR6 wherein n is from 0-5, y is from 0-5 and each x is independently from 1-5; R6, and R7 are each independently hydrogen, hydroxy, substituted or unsubstituted C1-4 alkyl, substituted or unsubstituted C1-4 alkoxy, or halogeno; and pharmaceutically acceptable salts and optically active isomers thereof.

Compositions and methods for treating patients suffering from a proliferation disorder characterized by an increased voltage gated ion-channel uptake are described. Included are compositions comprised of a compound selected from the group consisting of matrine, oxymatrine, artemisinin, agmatine, and vinpocetine.

The present invention relates to a topical skin care composition containing a safe and effective amount of a skin care active comprising agmatine, and its salt; a safe and effective amount of a first additional skin care active selected from the group consisting of BHT or BHA, hexamidine, cetyl pyridinium chloride, green tea catechins, phytosterols, ursolic acid, compounds derived from plant extracts, their salts and derivatives; and a dermatologically acceptable carrier for the agmatine composition. The present invention also relates to methods of using such agmatine compositions to regulate hair growth and the condition of mammalian skin. Said methods generally comprise the step of topically applying the composition to the skin of a mammal needing such treatment, a safe and effective amount of such compositions.

A cross-linkable monomer comprises a fumaric acid functional group having a first end and a second end, a first spacer group affixed to said first end and comprising at least repeating unit, a first terminal group affixed to said first spacer group, a second spacer group affixed to said second end and comprising at least one ethylene glycol repeating unit, and a second terminal group affixed to said second spacer group. A hydrogel formed by cross-linking the present monomer and a method for making the monomer. A method for forming a hydrogel, comprises the steps of a) synthesizing a copolymer of poly(propylene fumarate) (PPF) and poly(ethylene glycol (PEG) so as to produce P(PF-co-EG), b) synthesizing a PEG-tethered fumarate (PEGF), c) coupling agmatine sulfate to the PEGF to produce PEGF modified with agmatine (Agm-PEGF), and d) cross-linking the P(PF-co-EG) from step a) with Agm-PEGF from step c).

The invention provides gene transfer compositions using as the gene carrier a biodegradable polymer that contains one or more cationic alpha amino acids, such as arginine or agmatine. The compositions form a tight soluble complex with a poly nucleic acid suitable for transfecting target cells to effect translation of the cargo poly nucleic acid by the target cell. Thus, such compounds are useful both in vitro and in vivo.

The invention discloses a rapid analysis method of biogenic amine in fish. The common biogenic amine (nine types of biogenic amine comprising agmatine, histamine, tyramine, spermine, spermidine, 2-phenylethylamine, tryptamine, cadaverine and putrescine) is analyzed in a targeted manner by taking 5-sulfosalicylic acid as an extraction solvent, adopting bead agitating and grinding as an extraction method, taking benzoyl chloride as a derivatization reagent and adopting an LC (liquid chromatogram)-triple tandem quadrupole mass spectrometry. The biogenic amine is detected in a multi-reactions monitoring manner by taking ion pairs with optimum response, which are obtained by optimization, as quantitative ion pairs and taking ion pairs with following response as auxiliary qualitative ion pairs.According to the method, the sample extraction time and the biogenic amine derivation time can be greatly shortened, so that the analysis time of the whole analysis process is greatly shortened. The analysis method has the advantages of being simple in sample pretreatment, rapid in derivation, high in detection sensitivity, high in repeatability and the like.

A use method of agmatine or a pharmaceutically allowable salt thereof, and a pharmaceutical composition comprising the same are disclosed. The method and pharmaceutical composition of the present invention can effectively cure or prevent eye diseases preferably including glaucoma, retinopathy, and optic neuropathy associated with apoptosis in retinal ganglion cells (RGCs), particularly hypoxia-induced or tumor necrosis factor-α (TNF-α)-induced apoptosis.

The invention discloses a method for simultaneously determining 20 organic amines, biogenic amines and cations in a fruit juice beverage, belonging to the field of analytical chemistry. In the method, a conductivity inhibition-ion chromatography method is adopted, and under the condition of multi-stage gradient elution, the experimental determination conditions are optimized by researching a plurality of experimental influence factors, so that the method for simultaneously determining 20 organic amines, biogenic amines and cations (methylamine, dimethylamine, trimethylamine, diethanolamine, butylamine, histamine, cadaverine, putrescine, agmatine, heptanediamine, spermidine, spermine, Li+, Na+, NH4+, K+, Mg2+, Ca2+, Sr2+ and Ba2+) in the fruit juice beverage is established. The method is rapid, simple and convenient, high in high in sensitivity and good in stability, and achieves the aims of rapid analysis, green analysis and multi-component simultaneous analysis.

The invention discloses a method for synthesizing agmatine sulfate. The method comprises the following steps of: in a reaction solvent, performing a condensation reaction of 1,4-butanediamine and O-methylisourea sulfate at the temperature of between 20 DEG C below zero and 100 DEG C, utilizing the TLC to detect if the reaction is finished, filtering the reaction solution under vacuum, adding alcoholic solution of sulfuric acid into the obtained filtrate dropwise, adjusting a pH value of the filtrate to below 7, condensing the filtrate to dryness, and re-crystallizing residues to obtain the agmatine sulfate, wherein the reaction solvent is water or a C1 to C3alcohol, the adding mol ratio of the 1,4-butanediamine and the O-methylisourea sulfate is 1:1-1:2.0, and the volume dosage of the reaction solvent is 4 to 20ml/g based on the mass of the O-methylisourea sulfate. The method has the advantages of easy and safe operation, high reaction yield, low production cost, basically no emissionof three wastes, and bigger application values and social economic benefits.

The invention belongs to the technical field of biological catalysis, and relates to a biological preparation method of agmatine sulfate, which comprises the following steps: by using an L-arginine sulfate solution as a substrate raw material, carrying out decarboxylation reaction in the presence of a biocatalyst and a coenzyme to obtain agmatine sulfate, and discharging gas CO2, wherein the biocatalyst adopts L-arginine decarboxylase, and the coenzyme adopts phosphopyridoxal. The L-arginine sulfate solution used as the substrate is stirred and mixed with the L-arginine decarboxylase and the coenzyme (phosphopyridoxal) to perform decarboxylation reaction under certain conditions, thereby obtaining the agmatine sulfate. The conversion rate is greater than or equal to 98%, and the continuous production yield can reach 90%. The technical route has the advantages of simple preparation technique, high production efficiency, low cost and the like. The fermentation and conversion techniques of the recombinant L-arginine decarboxylase can achieve the industrial preparation level, thereby basically solving the problems of high chemical synthesis cost, low yield, and great environmental hidden danger and the like.

The present invention discloses a histone simulated gene vector and a preparation method and application thereof, and in particular relates to a histone imitated gene vector PCAH capable of gene delivery. The invention also provides a gene vector system which is obtained through electrostatic adsorption self-assembly between nucleic acids and a polymer synthesized from arginine and histidine basic amino acid decarboxylated products, agmatine, histamine and bis(acryloyl)cystamine. The invention also discloses the preparation method of the histone simulated gene vector and application of the histone simulated gene vector to gene therapy. The histone simulated gene vector has good cell penetration function, high cell transfection efficiency and low cytotoxicity, and provides an effective mean for gene therapy of diseases.

The invention discloses a biotransformation method of agmatine sulfate and belongs to the field of pharmaceutical intermediates. The method comprises steps as follows: L-arginine taken as a substrate and arginine decarboxylase taken as a biotransformation catalyst are subjected to a decarboxylic reaction to produce a coarse agmatine product, the coarse agmatine product is acidized with dilute sulfuric acid, and a coarse agmatine sulfate product is obtained. The method adopts the simple technological process, has no special requirement for equipment and is applicable to industrial production; compared with a simple chemical method, the method has the advantages that the reaction condition is mild, the operation is simple and convenient, the cost is lower, the pollution is low, and an enzyme catalytic reaction which is difficult to conduct in the chemical reaction can be finished.

The invention discloses a cationic high-polymer transgenic vector having good biocompatibility, degradability and high stability, and a preparation method thereof. A glucan molecule is used as a main chain, and agmatine and fatty acid bonds are respectively connected to the main chain of the glucan molecule through hydroxyl groups on the main chain. Guanylation glucan and primary amine of agmatine are subjected to nucleophilic reaction through tosylation glucan, and such copolymerization mode can not destroy the main chain of the glucan and can change the primary amine into secondary amine. Meanwhile, in order to improve the transfection efficiency, the nontoxic fatty acid is introduced to enhance the hydrophobic property of the glucan-agmatine polycation.

Compositions and methods for treating diseases or disorders associated with the metabolic syndrome using conjugates of bile acids with basic amino acids or a decarboxylated amino acid such as agmatine are provided. Further provided are bile acid-basic amino acid conjugates including chenodeoxycholic acid with an amino acid selected from arginine, lysine, histidine, ornithine or a decarboxylated amino acid such as agmatine.

The invention discloses a recombinant corynebacterium crenatum capable of producing agmatine, and application thereof, and belongs to the technical field of biology. The invention provides a recombinant corynebacterium crenatum C.crenatum SYPA5-5/pXMJ19-speA; the recombinant corynebacterium crenatum is inoculated into a fermentation culture medium containing glucose to perform shake-flask fermentation for 72 h; therefore, the yield of agmatine in fermentation liquid is up to 9.7 g/L; simultaneously, the content of a by-product, namely L-arginine, is only 0.23 g/L; the recombinant corynebacterium crenatum is inoculated into the fermentation culture medium containing glucose to perform fermentor fermentation for 72 h; therefore, the yield of agmatine in the fermentation liquid is up to 37.44g/L; simultaneously, the content of the by-product, namely L-arginine, is only 0.76 g/L; and furthermore, L-arginine does not need to be added in the whole fermentation process.

Disclosed is a performance-enhancing supplement for pulmonary administration wherein the supplement consists of a base e-liquid and a selected combination of supplement ingredients. The base liquid is selected from vegetable glycerin or propylene glycol, and the combination of ingredients is selected from at least three of pineapple lime extract, riboflavin, L-tyrosine, L-theanine, taurine, caffeine, agmatine sulfate, citrulline malate, and beetroot. The resulting performance-enhancing supplement is provided in a standard vape cartridge for use in an electronic cigarette device.

The invention discloses an arginine decarboxylase mutant and an application of the arginine decarboxylase mutant in production of agmatine, which belong to the technical field of biology. The invention provides arginine decarboxylase mutants I534D and D535W which are less influenced by product inhibition. The arginine decarboxylase mutant I534D is obtained by mutating isoleucine at the 534th siteof wild-type arginine decarboxylase of which the amino acid sequence is shown as SEQ ID NO.1 into aspartic acid. The half inhibition constant of the arginine decarboxylase can reach 0.66 mol/L and isincreased by 5.5 times compared with that of wild arginine decarboxylase; the arginine decarboxylase mutant D535W is obtained by mutating aspartic acid at the 535th site of wild type arginine decarboxylase with an amino acid sequence shown as SEQ ID NO.1 into tryptophan, the half inhibition constant of the arginine decarboxylase can reach 0.50 mol/L, and is improved by 4.2 times compared with thatof wild arginine decarboxylase.

The invention provides a synthesis method of agmatine sulfate. The method comprises the steps of carrying out addition reaction by taking 1,4-butanediamine as a raw material and a cyanamide water solution as a guanidination reagent to obtain agmatine, and forming salt with sulfuric acid to obtain agmatine sulfate. The synthesis method disclosed by the invention, by taking cyanamide water solution as the guanidination reagent, is moderate in reaction condition, easy to control, light in pollution, favorable for environmental protection, low in cost, and suitable for industrial production and application on a large scale.

The invention discloses a gene for improving the plant disease resistance and application thereof. The gene LeAct1 codes an agmatine transferase, which has a DNA (Deoxyribonucleic Acid) sequence shown as SEQ ID NO.1 in a sequence table, or has a DNA sequence capable of being hybridized with SEQ ID NO.1 under the strictly strict condition, or the gene LeAct1 codes a DNA sequence with the same amino acid sequence as the SEQ ID NO.1 in the sequence table. The gene is transformed and imported into a receiver plant genome; the agmatine transferase is expressed; and the disease resistance capability of a receiver plant can be improved. When the gene is applied to the agricultural biological technology breeding for improving the crop disease-resistant properties, the breeding period can be greatly shortened; and the breeding efficiency is improved.

The invention discloses a method for producing 1, 4-butanediamine by using recombinant escherichia coli, which belongs to the genetic engineering technology, and is characterized in that an arginine decarboxylase mutant and agmatine enzyme are successfully expressed by using a high-copy plasmid pXMJ19 to construct recombinant bacteria. Whole-cell transformation results show that the original bacteria do not have the capability of excessively accumulating 1, 4-butanediamine, and the recombinant bacteria can realize excessive accumulation of 1, 4-butanediamine; and meanwhile, based on the characteristic that the genetically engineered strain can excessively accumulate 1, 4-butanediamine, enzymatic conversion is successfully adopted to produce 1, 4-butanediamine by taking arginine as a substrate. IPTG (isopropyl-beta-d-thiogalactoside) is added in a cell culture process to induce enzyme expression, and magnesium ions and coenzyme pyridoxal phosphate are added in a transformation system according to enzymatic properties of the arginine decarboxylase. Finally, 72.6 g.L <-1 > 1, 4-butanediamine can be accumulated through 24-hour conversion, and the yield of agmatine serving as an intermediate product is 0.14 g/L.