22 results about "Agmatine" patented technology

The invention discloses an application of lactobacillus mucosa LMSJ001 or a metabolite agmatine of the lactobacillus mucosa LMSJ001 in preparation of a medicine for improving intestinal aging, and the application research of the lactobacillus mucosa LMSJ001 in a constructed aging mouse model finds that the lactobacillus mucosa LMSJ001 can be used for preparing a medicine for improving intestinal aging. Therapeutic gavage and application of Lactobacillus mucosa LMSJ001 in colon organs for intervention can significantly improve aging indexes, alleviate histological aging performance, promote intestinal peristalsis of aged mice, reduce secretion of inflammatory factors of the aged mice, improve intestinal epithelial barrier functions of the aged mice and improve intestinal dryness of the aged mice. The lactobacillus mucosa LMSJ001 can generate a metabolite agmatine through arginine decarboxylase, and agmatine can improve related indexes of proliferation and differentiation of intestinal stem cells and reduce expression of related indexes of senescence. Based on the unique functions, the lactobacillus mucosa LMSJ001 or the metabolite agmatine of the lactobacillus mucosa LMSJ001 can be cooperated or complemented with other means, is used for preparing pharmaceutical compositions or detection kits and the like, and has very wide application prospects.

The invention relates to the technical field of rice stress-resistant cultivation, and discloses a rice root system salt tolerance adjusting method based on exogenous spermidine, which comprises the following core steps: in the critical period of rice root system development, directionally applying a spermidine solution subjected to purity verification to a rice root system area, by accurately regulating and controlling the application concentration, frequency, duration and application environment conditions of spermidine, the osmotic regulation capability and the anti-oxidation system function of a rice root system in a high-salt environment with the soil conductivity larger than or equal to 2 dS / m are improved, the salt tolerance of the root system is remarkably improved, and then growth and development of rice are guaranteed. The spermidine is a straight-chain polyamine compound with good biocompatibility, the chemical purity is not lower than 98%, no heavy metal is left, and the contents of lead, cadmium and mercury are all smaller than or equal to 0.1 mg / kg. According to the invention, precise targeted regulation and control of the salt tolerance of the rice root system are realized, and the regulation effect is stable and remarkable.

The invention provides an engineering bacterium for producing spermidine and a preparation method of the engineering bacterium, and relates to the field of genetic engineering. According to the invention, arginine decarboxylase, aspartate kinase, aspartate-semialdehyde dehydrogenase, spermidine efflux protein (MdtI / MdtJ), carboxylaminopropyl agmatine dehydrogenase (CAPADH), carboxylaminopropyl agmatine decarboxylase (CAPADC) and aminopropyl agmatine urea hydrolase (APAUH) are subjected to overexpression in escherichia coli by using a gene engineering technology, and then the escherichia coli is subjected to enzyme deactivation, so that the escherichia coli is obtained. The genetically engineered bacterium capable of producing the spermidine can be obtained by simultaneously knocking out a spermidine transport system (PotBCAD), a spermidine-N-acetyltransferase (spermidine-N-acetyltransferase) and a homoserine dehydrogenase (homoserine dehydrogenase), so that the genetically engineered bacterium capable of producing the spermidine can be obtained. The engineering bacterium greatly reduces the production cost of spermidine, has a wide application prospect, and lays a foundation for green production of spermidine.

The invention relates to the technical field of membranes, in particular to an antibacterial nanofiltration membrane and a preparation method thereof. The method comprises the following steps: carrying out polyethyleneimine / tannic acid pretreatment on a polyethersulfone ultrafiltration membrane base material; sequentially carrying out first interfacial polymerization on a water phase containing piperazine, agmatine sulfate and taurine and an organic phase containing trimesoyl chloride; carrying out secondary water-phase re-leaching by adopting a taurine solution; preparing a crosslinking anchoring agent which retains 20%-22% of maleimide double bonds; the composite material and trimesoyl chloride are jointly used for anchoring-densification treatment; and finally, carrying out acid / alkali alternation and thermocuring. Through unique step design and functional component cooperation, a stable antibacterial and anti-pollution structure is constructed in the separation layer, and the problems that a traditional nanofiltration membrane is prone to biological pollution and an antibacterial agent is prone to loss are solved. The obtained membrane has the advantages of long-acting antibiosis, high flux recovery rate, high ion selectivity and excellent acid and alkali resistant cycle stability.

The invention relates to a method for obtaining crystallised foetal bovine serum by adding agmatine that, at a specific concentration, acts to increase cell production and increase protein expression

The invention belongs to the field of plant planting, and discloses a method for improving salt tolerance of wheat, which specifically comprises the following steps: 1) seed soaking: soaking wheat seeds with agmatine solution; and 2) cultivation: placing the wheat seeds soaked in the step 1) in a salt stress environment for germination culture. The concentration of the agmatine solution in the step 1) is 0.5-5 [mu] M. According to the method for improving the salt tolerance of the wheat, the wheat seeds are soaked through the small molecule compound agmatine, the germination capacity of the wheat seeds in the salt stress environment is enhanced, seedling growth is promoted, the method has the advantages of being simple in use method and low in cost, and reliable technical support is provided for wheat planting in saline-alkali soil. Meanwhile, the invention provides a new application of agmatine in preparation of a reagent for improving the salt tolerance of wheat, the application range of agmatine is expanded, and a new biological regulation agent choice is provided for salt-resistant cultivation of crops.

The invention provides a quality identification method and application of nettle mongolica for preparing an anti-hyperuricemia drug. The content of three anti-hyperuricemia active components, namely chlorogenic acid, New Zealand vitexin 2 and isovitexin, in nettle mongolica to be identified needs to be detected; the nettle at least meets one of the conditions that the contents of chlorogenic acid, New Zealand vitexin 2 and isovitexin are respectively greater than or equal to 2.0 mg / g, 1.5 mg / g and 1.5 mg / g, and when the total content of the three components is greater than or equal to 5.0 mg / g, the nettle can be used for preparing the anti-hyperuricemia medicine, the quality is high, and the application cost can be reduced. The three compound components have inhibition results on hyperuricemia key target proteins XOD and GLUT9, and the inhibition effect of the isovitexin on the two proteins is the best in the three compound components and is significant, so that the three compounds, especially the isovitexin, of the isovitexin, the New Zealand vitexin 2 and the chlorogenic acid, have the significant inhibition effect on the two proteins, and have the significant inhibition effect on the hyperuricemia key target proteins XOD and GLUT9 in the hyperuricemia key target proteins XOD and GLUT9 in the hyperuricemia key target proteins XOD and GLUT9 in the hyperuricemia key target proteins. The compound has a wide application prospect in preparation of anti-hyperuricemia drugs.

The present application provides a composition, a compound, a wolfberry agmatine enriched part and its neuroprotective use. The wolfberry agmatine enriched part extracted from wolfberry fruit has excellent neurocyte protection effect and prevention and treatment effect on Parkinson's disease. The wolfberry agmatine enriched part has simple extraction process and excellent effect, and its neuroprotective effect is not only superior to wolfberry fruit water extract and wolfberry polysaccharide enriched part, but also superior to each wolfberry agmatine monomer.

Provided herein is an exercise supporting nutritional supplement with an associated delivery platform, method, and system. As disclosed the exercise supporting nutritional supplement may comprise some or all of Agmatine Sulfate, Betaine Anhydrous, Choline (as Choline Bitartrate), Citrulline, Creatine Monohydrate, Beta Alanine, L-Tyrosine, Choline Bitartrate, Bovine Collagen, Caffeine Anhydrous, Pink Himalayan Salt, and Black Pepper Extract.

The invention belongs to the technical field of gene engineering, and particularly relates to an acyltransferase gene and application thereof. The invention provides an acyltransferase gene and application thereof. The nucleotide sequence of the acyltransferase gene HOVUSG0796800 is as shown in SEQ ID NO. 1 (sequence identifier number 1). Results of the invention show that the expression quantity of HOVUSG0796800 is significantly increased after a silicon fertilizer is applied, and the gene has significant positive correlation with the content of coumaroyl agmatine, the content of feruloyl tyramine, the content of feruloyl putrescine and the content of coumaroyl tyramine. In-vitro enzyme activity experiments prove that the gene can effectively regulate and control synthesis of the phenol amine substances. Therefore, the HOVUSG0796800 gene has an important function in plant phenol amine metabolism, and has a good application prospect in the aspects of enhancing crop stress resistance and improving plant quality.

The invention relates to a tetramethyl rhodamine (TAMRA)-labeled agmatine (AGM) fluorescent probe as well as a preparation method and application thereof, and relates to the technical field of biological medicines. A fluorescent group TAMRA is connected with AGM through a solid-phase synthesis method, the high-purity TAMRA-AGM probe is obtained after purification and identification, the high-purity TAMRA-AGM probe has similar biological characteristics with unmodified agmatine, and on the cellular level, the probe can effectively enter various types of intestinal epithelial cells in a short time under the condition of low dosage and is co-located on mitochondria; in a tissue level, the probe can effectively enter intestinal epithelial cells in a body and can carry out visual observation and semi-quantitative analysis on the intestinal epithelial cells; the method realizes visual tracking and accurate positioning of agmatine in cell and tissue levels, provides a key research tool for deeply illuminating the action mechanism of AGM in prevention and treatment of sepsis intestinal injury and other diseases, and has important scientific value and application prospect.

A cosmetic composition is disclosed comprising i) at least one of methionine and its derivatives selected from methionine, S-adenosylmethionine, and S-adenosylmethioninamine; and ii) at least one of additional compounds selected from ornithine, agmatine, and their derivatives; wherein the weight ratio of the amount of said methionine and its derivatives to the amount of said additional compound is from 1:30 to 16:1.

The application belongs to the field of genetic engineering and biological catalysis, and discloses an arginine decarboxylase mutant derived from Bacillus licheniformis and application of the arginine decarboxylase mutant in production of agmatine. The application takes wild-type arginine decarboxylase BLSpeA of Bacillus licheniformis DW2 as a parent, and obtains a mutant BLspeA with improved catalytic performance through site-directed mutagenesis A157V . The results show that the mutant BLspeA A157V of the application has an efficiency of 68.13% in converting L-arginine into agmatine in a catalytic system, which is about 19% higher than that of the wild-type arginine decarboxylase, indicating that the mutant has potential superiority in producing agmatine and has good industrial application prospect.

In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, the disclosure, in one aspect, relates to unit dose compositions comprising (a) L-ornithine or a pharmaceutically acceptable salt thereof; (b) L-norvaline or a pharmaceutically acceptable salt thereof; (c) agmatine or a pharmaceutically acceptable salt thereof; and (d) at least one of the following components: tongkat ali, Tribulus terrestris, horny goat weed, Panax notoginsing, maca root, lycopene, ashwagandha, or any combination thereof. In one aspect, the unit dose compositions can increase or enhance blood flow in a subject. In another aspect, the unit dose compositions can treat erectile dysfunction in a subject.

The application relates to the field of biotechnology and discloses a method for enhancing the white powdery disease resistance of wheat. The application also discloses the application of agmatine and a solution thereof in improving the white powdery disease resistance of wheat, wherein the concentration of the agmatine is 5-200 micromoles per liter. The method for enhancing the white powdery disease resistance of wheat is simple in operation and low in cost.

A solid supplement composition comprising at least one non-ester nitrate compound and at least one isolated amino acid compound, wherein the at least one isolated amino acid compound is agmatine, wherein the at least one isolated amino acid compound is a separate compound than the at least one non-ester nitrate compound, and wherein the solid supplement composition comprises an amount of nitrate ion (NO3—) that is at least about 0.05625 mg. A semisolid supplement composition comprising at least one non-ester nitrate compound and at least one isolated amino acid compound, wherein the at least one isolated amino acid compound is agmatine, wherein the at least one isolated amino acid compound is a separate compound than the at least one non-ester nitrate compound, and wherein the semisolid supplement composition comprises an amount of nitrate ion (NO3—) that is at least about 0.05625 mg.

The invention discloses a method for simultaneously and accurately determining the content of various biogenic amines in baijiu, the various biogenic amines such as nicotinamide, beta-phenylethylamine, agmatine, putrescine and the like in the baijiu are detected on the basis of a gas chromatography-triple quadrupole mass spectrometry technology, 1, 7-diaminoheptane is added as an internal standard in the pretreatment process, N, N-dimethylformamide is used as an internal standard, and the content of various biogenic amines in the baijiu is accurately determined by using N, N-dimethylformamide as an external standard. According to the method, a mixture of N, O-bis (trimethylsilyl) trifluoroacetamide and trimethylchlorosilane is used as a derivatization reagent, the pretreatment process is effectively simplified, and accurate, rapid and high-sensitivity determination of multiple biogenic amines in the Baijiu is realized by controlling the conditions of gas chromatography and triple quadrupole mass spectrometry. According to the method, the baijiu sample does not need to be extracted by an organic solvent and the pH value is not required to be adjusted, the pretreatment operation is extremely simple, the biogenic amine sensitivity and the quantitative accuracy are high, the stability and the reliability are good, and the phenomena that the pretreatment operation is complicated and the detection accuracy is poor when the biogenic amine in the baijiu is quantified are effectively improved.

The invention relates to a method for obtaining crystalline fetal calf serum by adding agmatine with a specific concentration. The method plays a role in increasing the cell yield and increasing the protein expression quantity per cell. The method also involves reconstitution into a specific form of liquid for use of the liquid without losing its original properties, allowing storage without ultra-low temperature chains and reducing the risk of contamination, thereby allowing precise dosing of the amount to be used.

An anti-anxiety composition includes 2 grams of Beta Alanine; 0.75 grams of agmatine sulfate; 0.75 grams of taurine; 0.75 grams of magnesium citrate; and 100 mg of l-theanine. A method for decreasing anxiety in a subject includes administering to the subject a drinkable liquid that includes 0.25-2 grams of Beta Alanine.

The present invention concerns novel spermidine and agmatine producing bacterial strains belonging to the species Bacillus subtilis, Bacillus pumilus and Bacillus altitudinis and preparations thereof as well as food, feed, pharmaceutical, cosmetical, agricultural and laundry compositions containing such strains or preparations thereof and concerns further generally the use of strains of the species Bacillus pumilus and Bacillus altitudinis to produce spermidine or agmatine, in particular in food, feed, pharmaceutical, cosmetical, agricultural, cleaning and laundry compositions.

The present invention relates to a test device for detecting agmatine in a liquid sample and a method of use thereof, wherein the test device comprises in order: a sample pad, a conjugate pad, a membrane, and an absorbent pad, wherein: the conjugate pad comprises an agmatine detection antibody conjugate comprising gold nanoparticles coupled to one or more agmatine detection antibodies via one or more first linkers, wherein the one or more agmatine detection antibodies selectively bind to agmatine, and the agmatine detection antibody conjugate is mobile; the membrane comprises in order from the conjugate pad: a test zone and a control zone, wherein the test zone comprises an agmatine carrier protein conjugate comprising agmatine coupled to a carrier protein via a second linker, and the control zone comprises a secondary antibody, wherein the secondary antibody is immobilized on the membrane, and the secondary antibody binds to the agmatine detection antibody.

The present invention relates to a method for obtaining crystallized fetal bovine serum by adding agmatine, which has the effect of increasing cell production and increasing protein expression per cell at a specific concentration. Furthermore, the method, with respect to a specific reconstitution form into a liquid, is shown for use without losing its original properties, allowing for storage without a cryogenic chain, reducing the risk of contamination, and enabling precise dosage.