Patents
Literature
4 results about "Aspartate kinase" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Aspartate kinase (aspartokinase, aspartic kinase) is an enzyme that catalyzes the phosphorylation of the amino acid aspartate. This reaction is the first step in the biosynthesis of three essential amino acids: methionine, lysine, and threonine, known as the "aspartate family". The gene for aspartokinase is present only in microorganisms and plants; it is not present in animals, which must obtain aspartate-family amino acids in their diet.
An engineered bacterium for producing ectoine, its preparation method and application
ActiveCN117417873BStable fermentationIncrease production intensityPyruvate synthesisMalate quinone oxidoreductase
This invention provides an engineered bacterium for producing ectoine, its preparation method, and its application. The engineered bacterium is prepared using a method comprising the following steps: using *Corynebacterium glutamicum* as the starting strain, feedback inhibition by aspartate kinase LysC is relieved, the activity of the gene lysE encoding lysine efflux permease is reduced, and the ectoine synthesis gene cluster ectABC is expressed. Based on this, the activity of any one or at least two of the following—diaminobutyrate acetyltransferase EctA, pyruvate carboxylase Pyc, or aspartate kinase LysC—is enhanced, and / or the activity of any one or at least two of the following—maloquinone oxidoreductase Mqo or homoserine dehydrogenase Hom—is reduced to further increase ectoine yield.
Owner:CATAYA BIO (SHANGHAI) CO LTD
An engineered bacterium for producing ectoine, its preparation method and application
This invention provides an engineered bacterium for producing ectoine, its preparation method, and its application. The engineered bacterium is prepared using the following steps: using Corynebacterium glutamicum as the starting strain, feedback inhibition of aspartate kinase LysC is relieved, the activity of the gene lysE encoding lysine efflux permease is reduced, and the ectoine synthesis gene cluster ectABC is expressed; based on this, the activity of any one or at least two combinations of aspartate semialdehyde dehydrogenase Asd, aspartate transaminase AspB, or 6-phosphogluconate dehydrogenase Gnd is enhanced to further increase the yield of ectoine.
Owner:CATAYA BIO (SHANGHAI) CO LTD
Construction, screening and application of aspartate kinase lysc full gene deep mutation
This disclosure provides the construction of a LysC scanning library for aspartate kinase using Retrotron editing technology, the screening of mutants, and their applications, belonging to the fields of biotechnology and genetic engineering technology. Specifically, this disclosure constructs a mutant library of an in situ whole-genome deep mutant library of aspartate kinase using novel Retrotron editing technology. The mutants in the library can significantly increase the production of amino acids such as lysine, threonine, and isoleucine.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI