20 results about "Citrate synthase" patented technology

The application belongs to the technical field of genetic engineering, and particularly relates to a citrate synthase gene AtCS The application relates to the application of a citrate synthase gene in regulating the anti-aging ability of seeds and a method for screening seed anti-aging agents. AtCS The application provides a citrate synthase gene AtCS The nucleotide sequence of the citrate synthase gene is shown as SEQ ID NO. 1. The citrate synthase gene AtCS (referred to as AtCS gene) encodes a citrate synthase located in mitochondria. AtCS The gene plays a key role in regulating the active oxygen homeostasis and anti-aging ability of seeds. AtCS The germination rate of the seed of the gene knockout mutant significantly decreases after aging, membrane lipid peroxidation products accumulate, and the active oxygen scavenging system is damaged. The mutant can be used for screening seed anti-aging agents, and provides a new solution for seed anti-aging.

The invention discloses a recombinant microorganism for producing hydroxytyrosol as well as a construction method and application of the recombinant microorganism. According to the recombinant microorganism, 3-deoxy-D-arabinoheptulose-7-phosphate synthetase aroGfbr, chorismate mutase / pre-benzoic acid dehydrogenase tyrAfbr, 4-hydroxyphenylacetic acid 3-hydroxylase HpaBC, phenylpyruvate decarboxylase ARO10 and alcohol dehydrogenase ADH6 are subjected to overexpression, and the recombinant microorganism can be used for preparing the recombinant microorganism. The genome of the gene naturally comprises a citrate synthase coding gene gltA and a tyrosine aminotransferase coding gene tyrB; the original promoter of the gltA and / or the original promoter of the tyrB are / is replaced by a time sequence promoter PrrnC-37. When the recombinant microorganism is used for producing hydroxytyrosol, the production efficiency can be improved, and the production cost of hydroxytyrosol can be reduced.

The invention discloses a genetically engineered bacterium for synthesizing L-glutamine by taking glucose as a carbon source and application of the genetically engineered bacterium. The genetically engineered bacterium takes escherichia coli as a chassis strain; according to the invention, a citrate synthase gene gltA, an aconitic acid hydratase gene can, an isocitrate dehydrogenase gene icd, a glutamate dehydrogenase gene gdh, a glutamine synthetase gene glnA and ammonium transporter genes amt and amtb can be overexpressed. Compared with the prior art, the invention provides a new strain and a new method for biosynthesizing L-glutamine, and the new strain and the new method have the significances of economy, scientificity, low cost, simple process and large-scale production.

The present disclosure relates to a modified polypeptide with attenuated activity of citrate synthase, a microorganism producing leucine comprising the modified polypeptide, and a method for producing an L-amino acid using the microorganism.

The application provides a citrate synthase mutant, a valine-producing strain and a construction method and application thereof. The serine (Ser) at the 243rd position of the citrate synthase mutant is mutated into tyrosine (Tyr), the glycine (Gly) at the 315th position is mutated into isoleucine (Ile), the arginine (Arg) at the 325th position is mutated into proline (Pro), and the asparagine (Asn) at the 370th position is mutated into tyrosine (Tyr), which are used for constructing a valine-producing strain. The new strain does not contain plasmids, has no growth defects, and does not need to be induced, and has the advantages of good genetic stability, high fermentation conversion rate and the like, and is an excellent strain capable of stably producing valine. The strain efficiently synthesizes valine from scratch with glucose as a substrate, and the yield of valine is significantly improved.

This invention discloses a laccase derived from *Coprinus gracilis*, its recombinant expression strain, and a highly efficient expression method, belonging to the fields of microbiology and bioengineering technology. Using *Aspergillus niger* MA70.15 as the experimental strain, this invention employs a random integration strategy to achieve pC3-P... citA -SP GlaA - lcc9‑ T trpC ‑pyrG The expression vector was integrated into the upstream region of the promoter of the host bacterium citrate synthase in the form of nine consecutive copies. To investigate the effects of microparticles and osmotic regulators in the fermentation medium on laccase production by recombinant Aspergillus niger, this invention added different types and concentrations of microparticles and osmotic regulators, resulting in a laccase activity of 1566.7 U / L in shake-flask fermentation of recombinant strain AnRcgL1 and 1961 U / L in a 1 L fermenter, successfully achieving efficient recombinant expression of laccase Lcc9 in Aspergillus niger.

The invention discloses a genetically engineered bacterium for producing monensin and application of the genetically engineered bacterium, and belongs to the technical field of metabolic engineering. The invention provides a recombinant cinnamon streptomyces capable of increasing the yield of monensin, the recombinant cinnamon streptomyces simultaneously overexpresses phosphofructokinase (pfk), citrate synthase (cs), methylmalonyl coenzyme A isomerase (mutB) and isobutyryl coenzyme A mutase (icm), and the result shows that the recombinant cinnamon streptomyces overexpresses acetyl coenzyme A synthesis and energy supply of the monensin. According to the method, synthesis precursors (methylmalonyl CoA and ethylmalonyl CoA) are increased, the yield of monensin can be increased without increasing the supply of the precursors, the yield can reach 20.4 g / L, the production cost is effectively reduced, and the production benefit is improved.

A nutrition recommendation system incorporating results from mitochondrial enzyme testing includes a data aggregation module arranged to receive mitochondrial enzyme testing test data from at least one of citrate synthase, Complex I, Complex II, Complex II plus Complex III, and Complex IV testing. A data processing and recommendation module receives and processes that test data, recommending at least one protocol comprising nutrient suggestions for mitochondrial health.

This invention relates to a compound microbial agent for promoting the germination of Dendrobium officinale seeds, belonging to the field of bio-fertilizer technology. It mainly includes Bacillus amyloliquefaciens, which dynamically constructs a constant microenvironment with a pH of 6.0 to 6.5 through metabolic synergy. Under acidic conditions, it induces carbonic anhydrase to generate calcium carbonate microcrystals to neutralize H⁺, and under alkaline conditions, it induces citrate synthase to secrete citrate and release H⁺, forming an "acid-base double buffer pair" with the mycelial network, thus controlling the pH fluctuation of the micro-region within ±0.3. This application also provides a method for preparing the microbial agent and its application technology, which significantly improves the germination rate and seedling quality of Dendrobium officinale seeds, while possessing advantages in ecological safety and economics, making it suitable for agricultural planting, ecological restoration, and other fields.

The invention belongs to the technical field of microorganisms, and particularly relates to a method for improving Iturin A production capacity of bacillus and a bacillus mutant strain. According to the specific technical scheme, the method comprises the following steps: carrying out gene modification on bacillus, and reducing the strength of a citrate synthase CitZ promoter in the bacillus, so as to obtain a bacillus mutant strain. According to the method, a promoter with proper strength is used for replacing a tricarboxylic acid cycle key rate-limiting enzyme citrate synthase CitZ promoter, and a fermentation regulation strategy of metabolic restriction-exogenous compensation is supplemented, so that the fermentation synthesis efficiency of the iturin A is remarkably improved finally. The method has universality, the yield of the iturin A produced by the bacillus can be increased by about 30% or above, and the method has important application value.

The application discloses a recombinant corynebacterium glutamicum with high yield of tetrahydropyrimidine and application thereof, and belongs to the technical field of bioengineering. S301Y By overexpressing aspartate kinase Lyc and aspartate semialdehyde dehydrogenase Asd, the supply of precursor aspartate semialdehyde can be increased. By inserting a repressor protein BetI binding sequence in the dapA promoter region of dihydropyridine dicarboxylic acid synthetase and overexpressing the repressor protein BetI, the content of by-product L-lysine in the fermentation liquor can be greatly reduced. By inserting a repressor protein LmrA binding sequence in the gltA promoter region of citrate synthase and overexpressing the repressor protein LmrA, the imbalance between cell growth and acid fermentation is solved, the supply of precursors oxaloacetate and acetyl CoA is increased, the yield of tetrahydropyrimidine is greatly improved, the yield is as high as 45.5 g / L, and the yield rate is as high as 0.25 g / g glucose.

The present application relates to a kind of 1,3-butanediol genetically engineered bacteria.The bacteria include the 5' end untranslated region (UTR) sequence that is artificially modified, which is the UTR sequence of the gene gltA encoding citrate synthase, and a constitutive promoter sequence is added before the artificially modified UTR sequence, which can enhance the accumulation of substrate acetyl-coa, reduce the acetyl-coa into the tricarboxylic acid cycle, and improve the yield of 1,3-butanediol.And by adding other genes to strengthen the synthesis of precursors, the chassis organism is further modified, and finally the obtained strain is a high-yield 1,3-butanediol genetically engineered bacteria.

Provided are a theanine-producing strain and use thereof in tea fermentation production. A Corynebacterium glutamicum is proposed, which includes an alanine decarboxylase CsAlaDC mutant. The theanine-producing strain is obtained by taking the Corynebacterium glutamicum as a starting strain, knocking out in sequence an α-ketoglutarate dehydrogenase E1 subunit gene odhA, a glutamate external transporter gene Ncg11221 and a lactate dehydrogenase gene ldh; and / or expressing a citrate synthase gene gltA, a pyruvate kinase gene pyk and a glutamate dehydrogenase gene gdh; and / or overexpressing an alanine dehydrogenase alaA and integrating a γ-glutamine synthetase GMAS into a cg1960 pseudogene locus of the Corynebacterium glutamicum.

The present disclosure relates to a type II citrate synthase variant, a Corynebacterium glutamicum strain comprising the type II citrate synthase variant, and a method for producing aspartate-derived L-amino acids by using the strain.

This invention discloses a method for constructing recombinant *Escherichia coli* using combinatorial protein engineering and metabolic engineering to efficiently synthesize (–)-α-biazinool, belonging to the field of bioengineering. This invention modifies the bisabolol synthase sequence to obtain the combinatorial mutant I364N / T258S / H479A, increasing the (–)-α-biazinool yield to 2.77 g / L. It also knocks out acetyl-CoA esterase and acetyl-CoA transporter, and inserts a DAS+4 degradation tag at the end of the citrate synthase gene. Based on this, the mutant T258S / I364N / H479A is transferred into it, increasing the (–)-α-biazinool yield to 3.08 g / L. Fermentation in a 5 L fermenter synthesizes (–)-α-biazinool with a yield of 7.41 g / L. The efficient synthesis of (–)-α-biazinool using glucose as the sole carbon source is achieved using an inorganic salt medium.

This disclosure belongs to the fields of biotechnology and genetic engineering, specifically disclosing a high-throughput screening and application of citrate synthase mutants. This disclosure utilizes precise retrotron editing technology of Corynebacterium glutamicum to obtain an in situ full-genome deep mutant library of citrate synthase. By employing a lysine biosensor for high-throughput screening of citrate synthase mutants, mutants that significantly increase the yield in the production of amino acids and their derivatives can be obtained, achieving stable and efficient production of amino acids and their derivatives.

A nutrition recommendation system incorporating results from mitochondrial enzyme testing includes a data aggregation module arranged to receive mitochondrial enzyme testing test data from at least one of citrate synthase, Complex I, Complex II, Complex II plus Complex III, and Complex IV testing. A data processing and recommendation module receives and processes that test data, recommending at least one protocol comprising nutrient suggestions for mitochondrial health.

The present disclosure relates to a citrate synthase variant, a microorganism comprising the variant, and a method for producing O-acetyl-L-homoserine and L-methionine using the microorganism.

The present invention relates to ancestor citrate synthase proteins capable of simultaneously forming at least 3 different complexation states. The invention also relates to compositions comprising the ancestor citric acid synthase protein, uses of the ancestor citric acid synthase protein or compositions, nucleic acids encoding the ancestor citric acid synthase protein, and methods of purifying the ancestor citric acid synthase protein.