9 results about "Citrate synthase" patented technology

A nutrition recommendation system incorporating results from mitochondrial enzyme testing includes a data aggregation module arranged to receive mitochondrial enzyme testing test data from at least one of citrate synthase, Complex I, Complex II, Complex II plus Complex III, and Complex IV testing. A data processing and recommendation module receives and processes that test data, recommending at least one protocol comprising nutrient suggestions for mitochondrial health.

This invention relates to a compound microbial agent for promoting the germination of Dendrobium officinale seeds, belonging to the field of bio-fertilizer technology. It mainly includes Bacillus amyloliquefaciens, which dynamically constructs a constant microenvironment with a pH of 6.0 to 6.5 through metabolic synergy. Under acidic conditions, it induces carbonic anhydrase to generate calcium carbonate microcrystals to neutralize H⁺, and under alkaline conditions, it induces citrate synthase to secrete citrate and release H⁺, forming an "acid-base double buffer pair" with the mycelial network, thus controlling the pH fluctuation of the micro-region within ±0.3. This application also provides a method for preparing the microbial agent and its application technology, which significantly improves the germination rate and seedling quality of Dendrobium officinale seeds, while possessing advantages in ecological safety and economics, making it suitable for agricultural planting, ecological restoration, and other fields.

The present application relates to a kind of 1,3-butanediol genetically engineered bacteria.The bacteria include the 5' end untranslated region (UTR) sequence that is artificially modified, which is the UTR sequence of the gene gltA encoding citrate synthase, and a constitutive promoter sequence is added before the artificially modified UTR sequence, which can enhance the accumulation of substrate acetyl-coa, reduce the acetyl-coa into the tricarboxylic acid cycle, and improve the yield of 1,3-butanediol.And by adding other genes to strengthen the synthesis of precursors, the chassis organism is further modified, and finally the obtained strain is a high-yield 1,3-butanediol genetically engineered bacteria.

The present disclosure relates to a type II citrate synthase variant, a Corynebacterium glutamicum strain comprising the type II citrate synthase variant, and a method for producing aspartate-derived L-amino acids by using the strain.

This invention discloses a method for constructing recombinant *Escherichia coli* using combinatorial protein engineering and metabolic engineering to efficiently synthesize (–)-α-biazinool, belonging to the field of bioengineering. This invention modifies the bisabolol synthase sequence to obtain the combinatorial mutant I364N / T258S / H479A, increasing the (–)-α-biazinool yield to 2.77 g / L. It also knocks out acetyl-CoA esterase and acetyl-CoA transporter, and inserts a DAS+4 degradation tag at the end of the citrate synthase gene. Based on this, the mutant T258S / I364N / H479A is transferred into it, increasing the (–)-α-biazinool yield to 3.08 g / L. Fermentation in a 5 L fermenter synthesizes (–)-α-biazinool with a yield of 7.41 g / L. The efficient synthesis of (–)-α-biazinool using glucose as the sole carbon source is achieved using an inorganic salt medium.

This disclosure belongs to the fields of biotechnology and genetic engineering, specifically disclosing a high-throughput screening and application of citrate synthase mutants. This disclosure utilizes precise retrotron editing technology of Corynebacterium glutamicum to obtain an in situ full-genome deep mutant library of citrate synthase. By employing a lysine biosensor for high-throughput screening of citrate synthase mutants, mutants that significantly increase the yield in the production of amino acids and their derivatives can be obtained, achieving stable and efficient production of amino acids and their derivatives.

The present disclosure relates to a citrate synthase variant, a microorganism comprising the variant, and a method for producing O-acetyl-L-homoserine and L-methionine using the microorganism.