Kit for and method for content determination of acetyl coenzyme A
A technology of acetyl coenzyme and determination method, applied in biochemical equipment and methods, determination/inspection of microorganisms, etc., can solve the problems of low content of acetyl coenzyme A, low sensitivity, difficult detection, etc., to simplify detection steps and shorten reaction time. , to measure the effect of simple and quick
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Embodiment 1
[0069] A kit for measuring acetyl-CoA content, comprising the following reagents:
[0070] Reagent 1, in liquid form, stored at 4°C, consists of polyvinylpyrrolidone (PVP) with a mass of 2.5g, mercaptoethanol with a volume of 37uL, a concentration of 100mM, phenylmethylsulfonyl fluoride (PMSF) with a volume of 500uL and a volume of Dissolve 5mL of glycerol in Tris-HCl buffer solution with a concentration of 50mM, pH=7.5, and a volume of 100mL, and place it in a reagent bottle;
[0071] Reagent 2, in powder form, stored at -20°C, composed of malate dehydrogenase (MDH) with a mass of 0.3mg, placed in a 1.5mLEP tube;
[0072] Reagent 3, in liquid form, stored at 4°C, composed of citrate synthase with a volume of 10uL, placed in a 1.5mLEP tube;
[0073] Reagent 4, in powder form, stored at -20°C, composed of 30 mg of malic acid and 7.5 mg of oxidized coenzyme I (NAD), placed in a 30 mL reagent bottle;
[0074] Reagent 5, in liquid form, stored at 4°C, consists of a Tris-HCl buff...
Embodiment 2
[0108] A kit for measuring acetyl-CoA content, comprising the following reagents:
[0109] Reagent 1, in liquid form, stored at 4°C, consists of polyvinylpyrrolidone (PVP) with a mass of 2.5g, mercaptoethanol with a volume of 37uL, a concentration of 100mM, phenylmethylsulfonyl fluoride (PMSF) with a volume of 500uL and a volume of Dissolve 5mL of glycerol in Tris-HCl buffer solution with a concentration of 50mM, pH=7.5, and a volume of 100mL, and place it in a reagent bottle;
[0110] Reagent 2, in powder form, stored at -20°C, composed of malate dehydrogenase (MDH) with a mass of 0.3mg, placed in a 1.5mLEP tube;
[0111] Reagent 3, in liquid form, stored at 4°C, composed of citrate synthase with a volume of 10uL, placed in a 1.5mLEP tube;
[0112] Reagent 4, in powder form, stored at -20°C, composed of 30 mg of malic acid and 7.5 mg of oxidized coenzyme I (NAD), placed in a 30 mL reagent bottle;
[0113] Reagent 5, in liquid form, stored at 4°C, consists of a Tris-HCl buff...
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