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Ferrous ion diagnosis/determination reagent kit and method for determining ferrous ion concentration

A technology of ferrous ion and kit, applied in the field of measuring ferrous ion concentration, ferrous ion diagnosis/measurement kit

Inactive Publication Date: 2008-12-17
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there is still a reaction between the application of chromazurin S and high iron

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The ferrous ion diagnostic / determination reagent in this embodiment is a single reagent, including:

[0043] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0044] Stabilizer 500mmol / L

[0045] Reduced coenzyme 0.25mmol / L

[0046] Aconitic acid 10mmol / L

[0047] Coenzyme A 5mmol / L

[0048] Carbon dioxide 30mmol / L

[0049] Aconitase 6000U / L

[0050] Citrate synthase 12000U / L

[0051] Pyruvate dehydrogenase 18000U / L

[0052] After all the reagents are dissolved and prepared, they are divided into bottles and freeze-dried to prepare dry powder reagents; before use, add pure water and use after reconstitution.

[0053] Set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test sub-wavelength 405nm, the volume ratio of the measured ferrous ion sample to the reagent is 1 / 25. The reaction direction is negative reaction (declining reaction), the delay time is about 2 minute...

Embodiment 2

[0056] The ferrous ion diagnostic / determining reagent in this embodiment is a dual reagent, including:

[0057] Reagent 1

[0058] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0059] Reduced coenzyme 0.25mmol / L

[0060] Aconitic acid 10mmol / L

[0061] Coenzyme A 5mmol / L

[0062] Carbon dioxide 30mmol / L

[0063] Reagent 2

[0064] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0065] Stabilizer 500mmol / L

[0066]Aconitase 6000U / L

[0067] Citrate synthase 12000U / L

[0068] Pyruvate dehydrogenase 18000U / L

[0069] After all the reagents are dissolved and prepared, they are divided into bottles to form a liquid double reagent, which can be used directly.

[0070] Set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test sub-wavelength 405nm, volume of ferrous ion sample and reagent 1, reagent 2 The ratio is 2 / 20 / 5, the reaction direction is negative r...

Embodiment 3

[0073] The ferrous ion diagnostic / determination reagent in this embodiment is three reagents, including:

[0074] Reagent 1

[0075] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0076] Stabilizer 500mmol / L

[0077] Reduced coenzyme 0.25mmol / L

[0078] Aconitic acid 10mmol / L

[0079] Coenzyme A 5mmol / L

[0080] Carbon dioxide 30mmol / L

[0081] Reagent 2

[0082] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0083] Stabilizer 500mmol / L

[0084] Citrate synthase 12000U / L

[0085] Pyruvate dehydrogenase 18000U / L

[0086] Reagent 3

[0087] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0088] Stabilizer 500mmol / L

[0089] Aconitase 6000U / L

[0090] After all the reagents are dissolved and prepared, they are divided into bottles to form three liquid reagents, which can be used directly.

[0091] When determining the ferrous ion concentration, set on the automatic biochemical analyzer: temperature 37℃, reaction time 10...

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Abstract

The invention relates to a kit for diagnosing / mensurating ferrous ions by utilizing the technologies of the enzymic colorimetric method and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the ferrous ions, and belongs to the technology field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, cis-aconitic acid, coenzyme A, carbon dioxide, reduced coenzyme, aconitase, citrate synthetase, pyruvate dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, an enzymatic reactions occurs, then the reactant is placed under an ultraviolet / visible light analyzer, and the velocity of the decrease in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the ferrous ions.

Description

Technical field [0001] The present invention relates to a ferrous ion diagnostic / determination kit. At the same time, the present invention also relates to a method for determining the concentration of ferrous ion, which belongs to the technical field of medical / food / environmental inspection and determination. Background technique [0002] The binding force of iron and total iron is extremely important in the process of human metabolism. Iron is an indispensable substance in the normal physiological process of the human body. The human body contains about 3 to 5g of iron, 70% of which are found in red blood cell hemoglobin; 25% are distributed in the mononuclear macrophage system (ie, reticuloendothelial system, ferritin and hemosiderin in liver, spleen, and bone marrow); 4.9% Distributed in myoglobin, cytochrome, iron-containing enzymes; 0.1% is the iron in circulating plasma. [0003] Iron in the human body comes from food and is mainly absorbed in the duodenum and upper small ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/84G01N21/31C12Q1/26C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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