49 results about "Genetic enhancement" patented technology

The invention provides recombinant Escherichia coli. The recombinant Escherichia coli comprises the following multiple modifications: knocking out genes such as iclR, lpxM, ldhA, nadR, ptsP and the like; the expression of genes such as narJ, setA and the like is enhanced. The Escherichia coli disclosed by the invention can rapidly intake a glucose raw material, and can rapidly grow under various culture conditions. Therefore, the method has potential application value in the field of fermentation.

The invention belongs to the technical field of synthetic biology, and particularly relates to recombinant escherichia coli for efficiently producing O-acetyl-L-homoserine as well as a construction method and application of the recombinant escherichia coli. According to the invention, CRISPR-Cas9 gene editing is combined with metabolic modification, so as to precisely integrate OAH biosynthesis key nodes: introducing and overexpressing a mutant metX gene at a genome pseudogene site to enhance the expression of homoserine acetyltransferase, knocking out byproducts such as poxB and mgsA to synthesize genes, and carrying out high-yield synthesis of OAH. Corynebacterium glutamicum thrA (anti-feedback inhibition), aspB and bacillus subtilis acsA-acuA genes (construction of an acetic acid switch) are introduced, gene expression of gltA, tpiA and the like is optimized to increase supply of precursors such as acetyl CoA and the like, and finally a stable strain without plasmids, antibiotics and inducers is obtained. The production cost is effectively reduced, and the method has the advantage of high genetic stability and shows a good application prospect in OAH industrial production.

The invention discloses a genetically engineered bacterium for efficiently producing D-pantothenic acid as well as a construction method and application of the genetically engineered bacterium, the construction method comprises the following steps: (a) taking corynebacterium glutamicum DPA2 as a chassis bacterium, enhancing the expression of at least one pantothenic acid main pathway gene in a chassis bacterium genome, and introducing plasmid pEC-XK99E-panBCilvC to obtain an engineered strain for modifying the pantothenic acid main pathway gene; (b) knocking out at least one heteroacid pathway gene in the engineering strain obtained in the step (a), carrying out in-situ integration on a panBCC gene at an alaT gene locus, and introducing a plasmid pEC-XK99E-panBCilvC to obtain a heteroacid pathway modified engineering strain; and (c) knocking out the pyruvic acid shunt gene pyc in the heteroacid pathway modified strain obtained in the step (b), enhancing the expression of an odx gene, and introducing a plasmid pEC-XK99E-panBCilvC to obtain the engineering strain for regulating and controlling the pyruvic acid shunt. The genetically engineered bacterium has the beneficial effects that the genetically engineered bacterium for efficiently producing D-pantothenic acid is obtained, and the yield and the conversion rate are higher; the level of the modified strain in the aspect of fermentation production of D-pantothenic acid is obviously improved.

Levels of expression of antibiotic resistance genes are increased up to six-fold by inserting a proteasome-targeting tag into transgenes expressed in eukaryotic cells. Various selectable marker proteins are combined with different destabilization domains, leading to up to 70% increase in transgene expression. The increase in expression varies highly depending on the engineered construct and the lines cells used. Increase in expression drives exosome loading of cargo proteins in some aspects. By increasing expression and by editing trafficking signals of cargo proteins, proteins that normally locate to the ER can be trafficked to exosomes. This disclosure discloses efficient exosome delivery of a wide variety of engineered proteins, including modified antigen proteins of SARS-CoV-2 and influenza, and other proteins such as a modified alpha galactosidase A, an extracellular domain of vascular endothelial growth factor fused to a constant region of a human immunoglobulin heavy chain, and modified trastuzumab heavy and light chains.

The invention belongs to the technical field of plant genetic engineering and crop breeding, and particularly relates to application of wheat lectin receptor-like kinase TaSIT2 and a coding gene TaSIT2 thereof in improving stripe rust resistance of crops. A wheat lectin receptor-like kinase gene TaSIT2 is analyzed and screened by using a reverse genetics method, a TaSIT2 gene editing wheat plant is obtained by using an agrobacterium-mediated genetic transformation method through a gene editing technology, the resistance of the transgenic plant to wheat stripe rust physiological races is remarkably enhanced, and the wheat stripe rust physiological races can be obtained. It is shown that knockout of the TaSIT2 gene enhances the resistance of wheat to stripe rust, it is determined that the wheat lectin receptor-like kinase TaSIT2 gene plays a negative regulation role in wheat stripe rust resistance, and a new technical thought and gene resources are provided for wheat stripe rust resistance variety cultivation from the perspective of molecular biology. And more paths are provided for cultivating lasting broad-spectrum disease-resistant varieties.

The invention discloses a method for improving the yield of L-threonine by enhancing Escherichia coli electronic respiration transfer and energy transfer efficiency, and belongs to the technical field of genetic engineering. According to the invention, a cytochrome bo oxidase subunit CyoA mutant derived from escherichia coli, a cytochrome bo oxidase subunit CyoB mutant derived from pseudomonas aeruginosa, a cytochrome bd oxidase cydAB gene cluster derived from escherichia coli and a polyphosphate kinase PPK mutant derived from radiation-resistant coccus are introduced, amn genes are knocked out, and a cytochrome bo oxidase gene is obtained. The electron transfer efficiency in escherichia coli and the production efficiency of energy ATP (adenosine triphosphate) are enhanced, and the production efficiency of L-threonine and the carbon yield are further improved. Finally, the obtained engineering strain ECTHRS-5 is subjected to fed-batch fermentation in a 5L fermentation tank to accumulate 160.8 g / L of L-threonine, and the sugar-acid conversion rate is 0.62 g / g.

The application belongs to the technical field of genetic engineering, and relates to a gene editing method for enhancing low-temperature tolerance of fish by targeting a trpv1 gene and application. The application precisely edits a fish trpv1 gene by using CRISPR-Cas9 technology, blocks negative signal transduction induced by low temperature, and releases the inhibition on related metabolic pathways. The application provides a brand-new molecular target for improvement of low-temperature tolerance of fish and cold-resistant breeding of aquatic products, and has important application value.

This invention discloses a method and its application for silencing plant disease-susceptible genes by loading double-stranded RNA (dsRNA) onto a three-dimensional mesoporous carrier of nanolayered carbon microcrystals, belonging to the field of nanopesticide formulation technology. After amino-functionalization modification, the carrier covalently couples with terminally carboxylated and 2′-O-methyl-modified dsRNA, achieving highly efficient loading (loading rate > 95%) through synergistic effects of π-π stacking, significantly improving the stability, leaf permeability, and resistance to nuclease degradation of the dsRNA. After delivering the dsRNA targeting the disease-susceptible gene to plant cells, it can specifically activate RNA interference, efficiently silencing the disease-susceptible gene and enhancing plant immunity. Further encapsulation in biodegradable polymer microcapsules can form a long-acting formulation with sustained-release properties. This invention provides an efficient, stable, and environmentally friendly RNA delivery strategy for plant disease control.

The invention discloses application of a soybean GmDsPTP12 gene in regulation and control of aluminum toxicity stress tolerance of plants, a nucleotide sequence of a CDS open reading frame of the GmDsPTP12 gene is as shown in SEQ ID NO: 1, and an amino acid sequence of a protein coded by the GmDsPTP12 gene is as shown in SEQ ID NO: 2. It is found that the soybean GmDsPTP12 gene can regulate and control the aluminum toxicity tolerance of soybeans, the aluminum toxicity tolerance of the soybeans is enhanced by overexpressing the soybean GmDsPTP12 gene, and the aluminum toxicity tolerance of the soybeans can be reduced by knocking out or interfering the expression of the soybean GmDsPTP12 gene.

The invention relates to a Rhodococcus ruber SD3 locen-2 protease-like protein 2 gene enhanced strain, which is a recombinant strain obtained by improving the expression of a gene s2plp2 of R.ruber SD3 in a R.ruber SD3 wild strain. The Rhodococcus ruber SD3 locen-2 protease-like protein 2 gene enhanced strain has the advantages that the expression of the gene s2plp2 of the R.ruber SD3 is improved; the method for constructing the enhanced strain comprises the following steps: carrying out PCR (Polymerase Chain Reaction) amplification on a gene s2plp2 of R.ruber SD3, and constructing a recombinant plasmid pNV18-s2plp2 carrying the gene s2plp2; the method comprises the following steps: carrying out recombinant escherichia coli expression on recombinant escherichia coli to obtain plasmids, transforming the plasmids into E. coli Top 10 competent cells, culturing a large amount of recombinant escherichia coli, extracting the plasmids, and electrically transforming the extracted plasmids into R.ruber SD3 competent cells to obtain the R.ruber s2plp2 gene enhanced strain R.ruber s2plp2-E. ruber s2plp2-E. ruber s2plp2-E. ruber s2plp2-E. ruber s2plp2-E. ruber s2plp2-E. ruber s2plp2. Compared with an R.ruber SD3 wild strain, the expression quantity of the s2plp2 gene in the R.ruber SD3 s2plp2 gene enhanced strain is obviously enhanced by 3.35 times, and the R.ruber SD3 s2plp2 gene enhanced strain shows stronger tolerance to phenol stress.

The present invention provides a benzoxazole small molecule compound, which is an inhibitor of the Zeste gene enhancer homolog 2 (EZH2) and can be used to prevent and / or treat EZH2-mediated related diseases, such as malignant tumors.

The invention belongs to the technical field of gene engineering, and particularly relates to a salt stress forward regulation gene BcCRK35 and application thereof. The nucleotide sequence of the BcCRK35 gene is as shown in SEQ ID NO. 1; the amino acid sequence is as shown in SEQ ID NO. 2; the gene can be used for regulating and controlling the salt tolerance of saccharomycetes and regulating and controlling the salt tolerance of non-heading Chinese cabbages. The gene is over-expressed in yeast, so that the tolerance of the yeast to salt stress is enhanced; the gene is knocked out or silenced, so that the salt tolerance of the non-heading Chinese cabbage is reduced. According to the invention, overexpression of constructed BcCRK35 yeast proves that the gene plays a positive regulation role in salt stress. Meanwhile, after the BcCRK35 gene is silenced, the tolerance of a silenced plant to salt stress is reduced. The invention lays a theoretical foundation for researching a plant salt stress response mechanism, also provides a gene resource for cultivating a new variety of salt-tolerant non-heading Chinese cabbage, and has a relatively good potential application value.

The application relates to the biotechnology field and discloses a drought stress related alfalfa gene and application thereof, and the MsCAS15 gene is cloned from alfalfa, the nucleotide sequence of which is shown as SEQ ID NO. 1, and the encoded dehydrin is shown as SEQ ID NO. 2. The protein is rich in glycine and lysine and has the functions of protecting cell membranes and biological macromolecules. The application obtains a transgenic plant overexpressing the MsCAS15 by constructing a recombinant expression vector containing the gene and using an agrobacterium-mediated method. The experimental results show that overexpression of the gene enhances the drought resistance of the plant, and the specific performance is that the seed germination rate of the transgenic plant is higher than that of the wild type under osmotic stress, and under the natural drought condition of soil, the wilting degree of the transgenic plant leaves is reduced, and the growth state is better than that of the wild type. The application provides an effective gene resource for cultivating new drought-resistant crop varieties.

The present invention relates to a preparation method for an amide compound and an application thereof in the field of medicine. Specifically, provided by the present invention is an amide small molecule compound which is an inhibitor of Zeste gene enhancer homolog 2 (EZH2) and which may be used to prevent and / or treat EZH2-mediated related diseases, comprising tumors, myeloproliferative diseases or autoimmune diseases.

The invention discloses a multiple PCR (Polymerase Chain Reaction) Panel for detecting favorable allelic variation of wheat yield related traits and application of the multiple PCR Panel. The multiplex PCR Panel takes 9 genetic synergistic sites related to wheat yield formation as targets and is used for performing targeted detection on corresponding favorable allelic variation. The nucleotide sequences of the primer group are as shown in SEQ ID No. 21 to SEQ ID No. 38. The PCR Panel can simultaneously and accurately analyze excellent allelic variation of a grain weight synergistic site, a grain width synergistic site and a plant height related site in a single reaction, and compared with a traditional marker detection mode taking a single gene or a single character as a target, the PCR Panel has the advantage that the analysis efficiency of multi-site genetic information can be remarkably improved. The multiple PCR Panel system is embedded into a conventional wheat breeding process, a cooperative breeding mode of'precise complementary matching of parents, primary screening of low-generation basic agriculture and targeted molecular selection of middle-generation and high-generation 'is constructed, rapid selection, polymerization and fixation of multiple sites of yield-related traits are realized, the breeding period is shortened, and the new germplasm creation efficiency is improved.

The invention relates to the technical field of gene engineering, in particular to a Cilp gene enhancer and application thereof. Through integration and verification of an ATAC-seq map, a single cell transcriptome data set and multi-omics data, the Cilp gene enhancer is obtained through screening, and a mouse inner ear microinjection system is utilized to further prove that the Cilp gene enhancer can significantly enhance the transcriptional activity of an EGFP protein gene in cochlea type 2 neuronal cells. Compared with other sequences with similar sizes, the Cilp gene enhancer provided by the invention can drive specific expression of a reporter gene or a Cilp gene in cochlear neurons, especially type 2 neurons, and the gene expression level is remarkably improved. The Cilp gene enhancer provided by the invention is suitable for biological materials such as recombinant DNA (deoxyribonucleic acid), vectors or adenoviruses, can be used for promoting transcription of EGFP (enhanced green fluorescent protein) genes or other genes in cochlea neurons, and provides a new targeting tool for gene therapy of hereditary hearing loss.

The invention discloses an application of an OsHDT702 gene in regulating and controlling the salt tolerance of rice. According to the application of the OsHDT702 gene in regulating and controlling the salt tolerance of the rice, the nucleotide sequence of the OsHDT702 gene is as shown in SEQ ID NO. 1. Through overexpression of the OsHDT702 gene, the salt tolerance of the rice is reduced; the salt tolerance of the rice is enhanced by knocking out the OsHDT702 gene. The method for improving the salt tolerance of the rice comprises the step that the OsHDT702 gene in the application is knocked out through a CRISPR / Cas9 technology, and the nucleotide sequence of a target spot is shown as SEQ ID NO.9. In the application, the OsHDT702 gene and the encoded protein thereof can regulate and control the salt tolerance of the rice. According to the application, the salt tolerance of the rice can be obviously improved by knocking out part of exon sequences in the gene, and the salt tolerance of the rice is obviously reduced by overexpressing the gene.

This invention discloses a genetically engineered strain of *Saccharomyces cerevisiae* and its application in cholesterol synthesis. The strain was obtained through multiple genetic engineering steps, starting with *Saccharomyces cerevisiae* SquP1: DHCR24 gene was integrated at the YPL062W site to achieve 7-dehydrocholesterol synthesis; the ERG6 promoter was replaced with the ERG7 promoter and the DWF5 gene was integrated to achieve cholesterol synthesis; the TGL3, TGL4, and ROX1 genes were knocked out to enhance cholesterol accumulation; the FLX1 and ZWF1 genes were integrated to strengthen cofactor supply; the HXK1 promoter was replaced and the ERG11 gene was integrated to optimize the synthesis pathway; and auxotrophic genes were reintroduced to obtain the CC37N strain. This strain achieved a maximum yield of 6.23 g / L in a 5L bioreactor fermentation, providing an excellent strain for efficient cholesterol biosynthesis.

The invention belongs to the technical field of genetic engineering, and particularly relates to an acetoin-producing lactic acid bacteria engineering bacterium, a construction method and application. The invention provides a lactobacillus engineering bacterium for producing acetoin. The basic bacterium of the lactobacillus engineering bacterium is lactobacillus casei; a lactic dehydrogenase gene 1 and a lactic dehydrogenase gene 2 are knocked out from a genome of the lactobacillus casei. The lactobacillus casei is taken as a basic bacterium, has the characteristics of GRAS safety, acid resistance and direct application potential in a food system, and can be used for flavor improvement, probiotic metabolism and postbiotic development. According to the invention, key lactic acid metabolic enzyme genes in lactobacillus casei are knocked out, an acetoin generation pathway is enhanced, acetoin can be stably and efficiently produced, and the engineering bacterium has good metabolic stability in a fermentation system and can be widely applied to the fields of food fermentation, natural perfume production and the like.

The invention relates to the field of plant genetic engineering, and discloses a method for enhancing citrus canker resistance by a citrus CsKCS6 gene. The citrus CsKCS6 gene is silenced, the resistance of citrus plants to citrus canker is improved, and the method specifically comprises the following steps: (1) cloning a VIGS fragment of the citrus CsKCS6 gene; (2) constructing an expression vector of VIGS of the CsKCS6 gene; and (3) transforming the citrus by the VIGS expression vector to obtain a citrus CsKCS6 gene silenced VIGS plant. The citrus CsKCS6 gene is obtained through cloning for the first time, the transcriptional level of citrus CsKCS6 is silenced and reduced through VIGS, and the function of CsKCS6 in citrus canker susceptibility resistance is verified; the method has a significant application value for citrus canker-resistant breeding, lays a foundation for citrus canker-resistant genetic breeding, and greatly promotes the development and application of citrus canker-resistant genetic engineering.

The invention discloses a pseudo-ginseng NBS-LRR coding gene PnCNL11 as well as application and a verification method of the pseudo-ginseng NBS-LRR coding gene PnCNL11. The nucleotide sequence of the pseudo-ginseng NBS-LRR coding gene PnCNL11 is SEQ ID NO: 1. The invention further discloses application of the NBS-LRR coding gene PnCNL11 of the pseudo-ginseng in improving the resistance of the pseudo-ginseng to pathogenic bacteria of root rot. The application verification method comprises the following steps: separating the NBS-LRR coding gene PnCNL11, verifying that the PnCNL11 has a positive regulation effect on infection of pathogenic bacteria of the root rot of the panax notoginseng by utilizing heterologous overexpression of tobacco and an RNAi interference experiment in the panax notoginseng, and enhancing the resistance of the root rot of the panax notoginseng by utilizing the NBS-LRR coding gene PnCNL11 of the panax notoginseng.

The application provides application of a white birch BpPIF4 gene in improving drought resistance of the white birch and belongs to the technical field of molecular biology.The application constructs an overexpression vector of the BpPIF4 gene, carries out genetic transformation on the white birch by using an agrobacterium infection method, screens a transgenic line overexpressing the BpPIF4 gene, and realizes overexpression of the white birch BpPIF4 gene.The application enhances the drought resistance of the white birch by overexpressing the white birch BpPIF4 gene, and can be used for cultivating forest trees with excellent quality.The BpPIF4 gene provided by the application can participate in drought resistance of the white birch, can be used for research on biological stress response of forest plants, and provides a reference resource for research on functions of a plant bHLH gene family.

The present invention relates to the field of tumor treatment technology, and specifically discloses the use of an HVEM inhibitor in the preparation of a drug for treating acute lymphoblastic leukemia. The present invention utilizes CRISPR / Cas9 gene editing technology to knock out the HVEM gene on tumor cells, thereby enhancing the body's anti-tumor immune response and significantly inhibiting tumor growth. Mechanistically, the present invention demonstrates that the anti-tumor immune response mediated by HVEM deficiency depends on CD8 + T cells, and HVEM, through its CRD1 domain, enables tumor cells to escape the body's immune surveillance, thereby promoting tumor progression. Furthermore, by knocking out the HVEM gene on tumor cells and combining it with doxorubicin chemotherapy, mice inoculated with acute lymphoblastic leukemia can achieve disease-free survival and induce immune memory in the body, effectively preventing tumor recurrence. This provides a new drug target for immunotherapy combined with chemotherapy for acute lymphoblastic leukemia.

The present application relates to the field of plant disease and pest control, in particular to the field of crop gene editing biological breeding. Specifically, it relates to a mutant maize zinnia-glucoside-oxygen-methyltransferase gene family, a coding protein and a method and use for controlling hemipteran pests, especially controlling aphid damage to plants. For the first time, the present application simultaneously targets and edits multiple paralogous genes of the maize zinnia-glucoside-oxygen-methyltransferase gene family through multi-target gene editing technology, thereby enhancing the aphid resistance level of maize plants throughout the whole growth cycle and the whole plant. The technical solution of the present application solves the problems in the prior art such as poor effect of chemical control on aphids, serious environmental pollution, and invalidity of Bt insecticidal proteins against aphids; can widely create new anti-aphid maize germplasm resources, solve the problem of lack of aphid-resistant maize germplasm, and provide an efficient, environmentally friendly and low-cost aphid control solution for maize production, which has important production application value.

This invention belongs to the fields of plant genetic engineering and crop breeding technology, specifically relating to wheat lectin receptor kinase TaSIT2 and its encoding gene. TaSIT2 Applications in improving crop resistance to stripe rust. This invention utilizes reverse genetics to analyze and screen wheat lectin receptor kinase genes. TaSIT2 Through gene editing technology and Agrobacterium-mediated genetic transformation, we can obtain... TaSIT2 Gene-edited wheat plants showed significantly enhanced resistance to wheat stripe rust races, indicating that knockout... TaSIT2 Genes enhanced wheat resistance to stripe rust and identified wheat lectin receptor kinases. TaSIT2 Genes play a negative regulatory role in wheat resistance to stripe rust, providing new technical ideas and gene resources for breeding wheat varieties resistant to stripe rust from a molecular biology perspective, and offering more pathways for breeding varieties with long-lasting and broad-spectrum disease resistance.

The application discloses a soybean Glyma.03G22970 gene enhancer regulatory element and an sgRNA molecule thereof. The enhancer regulatory element is an MHS2 regulatory element, and the nucleotide sequence of the MHS2 regulatory element is shown as SEQ ID NO:1. The sgRNA molecule has at least one of the nucleotide sequences shown as SEQ ID NO:3-4, and optionally has at least one of the nucleotide sequences shown as SEQ ID NO:2 and SEQ ID NO:5. The MHS2 regulatory element can be used as an enhancer of the Glyma.03G22970 gene, reduces the expression amount of the Glyma.03G22970 gene, and improves the oil content of soybean. The sgRNA molecule can make the sequence of the MHS2 regulatory element partially or completely missing, and has the advantages of low off-target rate, high editing efficiency and the like.

The invention discloses a Gjb2 gene enhancer and application thereof, and belongs to the field of gene engineering, the enhancer can significantly enhance the expression of a Gjb2 gene or other genes (such as a GFP gene) in an inner ear supporting cell, and can be used for gene therapy of deafness by utilizing the ability of the enhancer to specifically enhance the gene expression in the inner ear supporting cell; the Gjb2 gene enhancer is integrated and prepared into recombinant DNA containing the Gjb2 gene enhancer, a carrier or an adenovirus and other biological materials, gene expression regulation and control research is carried out, and the application prospect is good.

The present invention discloses a method for predicting key transcription factors based on enhancer and promoter data, which comprises the following steps: obtaining a FASTA format file of a gene enhancer sequence; obtaining a FASTA format file of a gene promoter sequence based on the positional information of the gene transcription start site; importing the FASTA format files of the gene enhancer sequence and the promoter sequence into the transcription factor online prediction system PROMO respectively, to obtain a transcription factor that targets the enhancer and a transcription factor that targets the promoter; screening out integrated transcription factors that simultaneously target the enhancer and the promoter; constructing a protein interaction network of the integrated transcription factors; screening transcription factors in core positions as key transcription factors that regulate gene expression. The present invention can accurately and quickly predict key transcription factors that regulate gene expression by integrating enhancer and promoter data, and has higher prediction accuracy.

The invention belongs to the technical field of microbial metabolism and genetic engineering, and relates to construction and application of engineering bacteria for producing L-homoserine. The construction method of the high-yield L-homoserine engineering bacteria provided by the invention is characterized in that escherichia coli (E.coli Bio-67727) for producing threonine is used as an original strain; a homoserine kinase coding gene thrB and a homoserine O-succinyltransferase coding gene metA are knocked out, and the expression of aspartate kinase I ThrA, aspartate semialdehyde dehydrogenase Asdbs, aspartate kinase III LysCpa, glucokinase Glk and a homoserine extracellular transport protein RhtA is enhanced. The engineering bacteria constructed by the invention can accumulate 156.2 g / L of L-homoserine after being fermented for 80 hours. The engineering bacterium does not carry plasmids, induction is not needed in the fermentation process, control is easy, and application to industrial production is facilitated.

The application discloses a method for enhancing the resistance of late-kumquat to citrus canker by a citrus CsATL66 gene, and relates to the technical field of plant genetic engineering, and the method comprises the following steps: cloning a VIGS fragment of the citrus CsATL66 gene and constructing a VIGS expression vector of a CsATL66 coding sequence, and then transforming the citrus by using an Agrobacterium tumefaciens mediation method, so that the transcription level of the CsATL66 in the transgenic citrus is successfully reduced, and a VIGS plant in which the citrus CsATL66 gene is silenced is obtained. The citrus CsATL66 gene is cloned for the first time, the transcription level of the citrus CsATL66 is reduced by using VIGS silencing, and the function of the CsATL66 in the resistance and susceptibility of the citrus to the citrus canker is verified. The application has great application value for the breeding of the citrus to resist the citrus canker, lays a foundation for the genetic breeding of the citrus to resist the citrus canker, and greatly promotes the development and application of the gene engineering of the citrus to resist the citrus canker.