83 results about "Genetic enhancement" patented technology

The invention provides recombinant Escherichia coli. The recombinant Escherichia coli comprises the following multiple modifications: knocking out genes such as iclR, lpxM, ldhA, nadR, ptsP and the like; the expression of genes such as narJ, setA and the like is enhanced. The Escherichia coli disclosed by the invention can rapidly intake a glucose raw material, and can rapidly grow under various culture conditions. Therefore, the method has potential application value in the field of fermentation.

The invention relates to the technical field of genetic engineering, and discloses a genetically engineered bacterium for producing L-arginine as well as a construction method and application of the genetically engineered bacterium. Escherichia coli is taken as a starting strain, and the following treatment is performed: overexpressing genes gdhA and gdh of glutamate dehydrogenase, promoting generation of glutamic acid, overexpressing and coding arginine transporter gene argO to enhance extracellular transport of arginine, introducing exogenous argJ to replace a linear synthesis pathway in large intestines, improving the utilization rate of acetyl coenzyme A and accelerating the synthesis efficiency of arginine; a glutamine synthetase gene glnA is over-expressed, and glutamine synthesis is enhanced; the pckA is overexpressed to promote ATP synthesis; meanwhile, speA and adiA genes for coding the arginine decarboxylase are deleted, so that further synthesis of putrescine from L-arginine is inhibited, and the L-arginine accumulation capability of the strain is improved. Through the combined action of the treatment, a genetically engineered bacterium with extremely high L-arginine production yield is finally obtained, and the shake flask fermentation yield can reach 13.7 g / L.

The invention discloses an Lhfpl5 gene enhancer and application thereof, and belongs to the field of gene engineering. According to the invention, the Lhfpl5 gene enhancer is found, the enhancer can obviously enhance the expression of the Lhfpl5 gene or other genes in inner ear internal and external hair cells, and the gene enhancer can be used for gene therapy of deafness by utilizing the capability of specifically enhancing the gene expression in the inner ear internal and external hair cells; the recombinant DNA containing the Lhfpl5 gene enhancer, or a carrier and other biological materials are prepared through integration, gene expression regulation and control research is carried out, and the application prospect is good.

The invention belongs to the technical field of synthetic biology, and particularly relates to recombinant escherichia coli for efficiently producing O-acetyl-L-homoserine as well as a construction method and application of the recombinant escherichia coli. According to the invention, CRISPR-Cas9 gene editing is combined with metabolic modification, so as to precisely integrate OAH biosynthesis key nodes: introducing and overexpressing a mutant metX gene at a genome pseudogene site to enhance the expression of homoserine acetyltransferase, knocking out byproducts such as poxB and mgsA to synthesize genes, and carrying out high-yield synthesis of OAH. Corynebacterium glutamicum thrA (anti-feedback inhibition), aspB and bacillus subtilis acsA-acuA genes (construction of an acetic acid switch) are introduced, gene expression of gltA, tpiA and the like is optimized to increase supply of precursors such as acetyl CoA and the like, and finally a stable strain without plasmids, antibiotics and inducers is obtained. The production cost is effectively reduced, and the method has the advantage of high genetic stability and shows a good application prospect in OAH industrial production.

The invention discloses a liriodendron hybrids LhCOLD1 gene as well as an expression protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the LhCOLD1 gene disclosed by the invention is shown as SEQ ID NO. 1, and the amino acid sequence of the expression protein of the LhCOLD1 gene is shown as SEQ ID NO. 2. The method comprises the following steps: constructing an overexpression vector of a hybrid liriodendron LhCOLD1 gene, and transforming the overexpression vector into arabidopsis thaliana and hybrid liriodendron calluses; and cultivating and screening to obtain a transgenic line with improved low-temperature stress resistance. After the LhCOLD1 transgenic arabidopsis thaliana plant is subjected to low-temperature treatment at-10 DEG C for 3 hours and the LhCOLD1 transgenic arabidopsis thaliana plant recovers to grow at room temperature for two weeks, the growth of the LhCOLD1 transgenic After the LhCOLD1 transgenic hybrid liriodendron tulipifera strain is treated at the low temperature of-10 DEG C for 1.5 hours, the influence of low-temperature stress on the growth is small. The result shows that the LhCOLD1 gene enhances the tolerance of the plant to low-temperature stress.

The present invention discloses a Mitf gene enhancer and its application in improving the gene-specific expression of cochlear stria vascular cells, and relates to the field of gene expression and regulation technology. The nucleotide sequence of the enhancer is shown in SEQ ID No. 1. The mouse embryo microinjection system was used to confirm that the sequence is an enhancer, which can significantly enhance the transcription activity of the EGFP protein gene in the cochlear stria vascular cells. Compared with other sequences of similar size, the enhancer sequence fragment can drive the reporter gene or Mitf to be specifically expressed in the cochlear stria vascular cells and the expression level is significantly enhanced. The enhancer is suitable for biological materials such as recombinant DNA, vectors or adenoviruses, and is used to promote the transcription of the EGFP gene or other genes in the cochlear stria vascular cells.

The invention discloses a soybean Glyma. 03G22970 gene enhancer regulatory element and a sgRNA (small guide ribonucleic acid) molecule of the soybean Glyma. 03G22970 gene enhancer regulatory element. The enhancer regulatory element is an MHS2 regulatory element, and the nucleotide sequence of the MHS2 regulatory element is as shown in SEQ ID NO: 1; the sgRNA molecule has at least one of nucleotide sequences as shown in SEQ ID NO: 3-4, and optionally has at least one of nucleotide sequences as shown in SEQ ID NO: 2 and SEQ ID NO: 5. The MHS2 regulatory element can be used as an enhancer of the Glyma. 03G22970 gene, so that the expression quantity of the Glyma. 03G22970 gene is reduced, and the oil content of soybeans is increased; the sgRNA molecule can partially or completely delete the sequence of the MHS2 regulatory element, and has the advantages of low miss rate, high editing efficiency and the like.

The invention discloses a method for producing phloretin by using saccharomyces cerevisiae, and belongs to the technical field of microbial genetic engineering. A natural metabolic phloretin path in a plant is successfully constructed in the saccharomyces cerevisiae BY4741, synthesis of phloretin in microorganisms is achieved, synthesis of the phloretin in the microorganisms is enhanced by enhancing synthesis path genes of tyrosine and phenylalanine in the saccharomyces cerevisiae, including elimination of tyrosine feedback inhibition and introduction of exogenous genes, synthesis of prerequisite tyrosine and phenylalanine is enhanced, and the yield of the phloretin is increased. And the yield of the phloretin is further improved by enhancing the expression of a key gene in a phloretin synthesis pathway. The yield of phloretin synthesized from the beginning by the engineering yeast can reach 246.21 mg / L, the synthesis problem of phloretin serving as a carbohydrase inhibitor is effectively solved, and a foundation is laid for synthesizing phloretin through efficient fermentation of microorganisms.

The invention discloses a genetically engineered bacterium for efficiently producing D-pantothenic acid as well as a construction method and application of the genetically engineered bacterium, the construction method comprises the following steps: (a) taking corynebacterium glutamicum DPA2 as a chassis bacterium, enhancing the expression of at least one pantothenic acid main pathway gene in a chassis bacterium genome, and introducing plasmid pEC-XK99E-panBCilvC to obtain an engineered strain for modifying the pantothenic acid main pathway gene; (b) knocking out at least one heteroacid pathway gene in the engineering strain obtained in the step (a), carrying out in-situ integration on a panBCC gene at an alaT gene locus, and introducing a plasmid pEC-XK99E-panBCilvC to obtain a heteroacid pathway modified engineering strain; and (c) knocking out the pyruvic acid shunt gene pyc in the heteroacid pathway modified strain obtained in the step (b), enhancing the expression of an odx gene, and introducing a plasmid pEC-XK99E-panBCilvC to obtain the engineering strain for regulating and controlling the pyruvic acid shunt. The genetically engineered bacterium has the beneficial effects that the genetically engineered bacterium for efficiently producing D-pantothenic acid is obtained, and the yield and the conversion rate are higher; the level of the modified strain in the aspect of fermentation production of D-pantothenic acid is obviously improved.

Levels of expression of antibiotic resistance genes are increased up to six-fold by inserting a proteasome-targeting tag into transgenes expressed in eukaryotic cells. Various selectable marker proteins are combined with different destabilization domains, leading to up to 70% increase in transgene expression. The increase in expression varies highly depending on the engineered construct and the lines cells used. Increase in expression drives exosome loading of cargo proteins in some aspects. By increasing expression and by editing trafficking signals of cargo proteins, proteins that normally locate to the ER can be trafficked to exosomes. This disclosure discloses efficient exosome delivery of a wide variety of engineered proteins, including modified antigen proteins of SARS-CoV-2 and influenza, and other proteins such as a modified alpha galactosidase A, an extracellular domain of vascular endothelial growth factor fused to a constant region of a human immunoglobulin heavy chain, and modified trastuzumab heavy and light chains.

The invention discloses a soybean Glyma. 05g029800 gene enhancer regulatory element and a sgRNA (small guide ribonucleic acid) molecule of the soybean Glyma. 05g029800 gene enhancer regulatory element. The enhancer regulatory element is an MHS beta regulatory element, and the nucleotide sequence of the MHS beta regulatory element is as shown in SEQ ID NO: 1; the sgRNA molecule has at least one of nucleotide sequences as shown in SEQ ID NO: 3-5, and at least one of nucleotide sequences as shown in SEQ ID NO: 2 and SEQ ID NO: 6. The MHS beta regulatory element can be used as an enhancer of the Glyma. 05g029800 gene, and after the MHS beta regulatory element is knocked out, the expression quantity of the Glyma. 05g029800 gene is reduced, and the nodulation quantity of soybeans is increased; the sgRNA molecule can enable a nucleotide sequence of an MHS beta regulation element to be deleted in a large fragment mode, and has the advantages of being low in off-target rate, high in editing efficiency and the like.

The invention belongs to the technical field of plant genetic engineering and crop breeding, and particularly relates to application of wheat lectin receptor-like kinase TaSIT2 and a coding gene TaSIT2 thereof in improving stripe rust resistance of crops. A wheat lectin receptor-like kinase gene TaSIT2 is analyzed and screened by using a reverse genetics method, a TaSIT2 gene editing wheat plant is obtained by using an agrobacterium-mediated genetic transformation method through a gene editing technology, the resistance of the transgenic plant to wheat stripe rust physiological races is remarkably enhanced, and the wheat stripe rust physiological races can be obtained. It is shown that knockout of the TaSIT2 gene enhances the resistance of wheat to stripe rust, it is determined that the wheat lectin receptor-like kinase TaSIT2 gene plays a negative regulation role in wheat stripe rust resistance, and a new technical thought and gene resources are provided for wheat stripe rust resistance variety cultivation from the perspective of molecular biology. And more paths are provided for cultivating lasting broad-spectrum disease-resistant varieties.

The invention discloses a method for improving the yield of L-threonine by enhancing Escherichia coli electronic respiration transfer and energy transfer efficiency, and belongs to the technical field of genetic engineering. According to the invention, a cytochrome bo oxidase subunit CyoA mutant derived from escherichia coli, a cytochrome bo oxidase subunit CyoB mutant derived from pseudomonas aeruginosa, a cytochrome bd oxidase cydAB gene cluster derived from escherichia coli and a polyphosphate kinase PPK mutant derived from radiation-resistant coccus are introduced, amn genes are knocked out, and a cytochrome bo oxidase gene is obtained. The electron transfer efficiency in escherichia coli and the production efficiency of energy ATP (adenosine triphosphate) are enhanced, and the production efficiency of L-threonine and the carbon yield are further improved. Finally, the obtained engineering strain ECTHRS-5 is subjected to fed-batch fermentation in a 5L fermentation tank to accumulate 160.8 g / L of L-threonine, and the sugar-acid conversion rate is 0.62 g / g.

The application belongs to the technical field of genetic engineering, and relates to a gene editing method for enhancing low-temperature tolerance of fish by targeting a trpv1 gene and application. The application precisely edits a fish trpv1 gene by using CRISPR-Cas9 technology, blocks negative signal transduction induced by low temperature, and releases the inhibition on related metabolic pathways. The application provides a brand-new molecular target for improvement of low-temperature tolerance of fish and cold-resistant breeding of aquatic products, and has important application value.

This invention discloses a method and its application for silencing plant disease-susceptible genes by loading double-stranded RNA (dsRNA) onto a three-dimensional mesoporous carrier of nanolayered carbon microcrystals, belonging to the field of nanopesticide formulation technology. After amino-functionalization modification, the carrier covalently couples with terminally carboxylated and 2′-O-methyl-modified dsRNA, achieving highly efficient loading (loading rate > 95%) through synergistic effects of π-π stacking, significantly improving the stability, leaf permeability, and resistance to nuclease degradation of the dsRNA. After delivering the dsRNA targeting the disease-susceptible gene to plant cells, it can specifically activate RNA interference, efficiently silencing the disease-susceptible gene and enhancing plant immunity. Further encapsulation in biodegradable polymer microcapsules can form a long-acting formulation with sustained-release properties. This invention provides an efficient, stable, and environmentally friendly RNA delivery strategy for plant disease control.

The invention discloses an immune tolerance realization method based on immune cell negative selection, which comprises the following steps: step 1, separating mononuclear cells from peripheral blood of a patient, and inducing and differentiating the mononuclear cells into DCs in vitro; 2, introducing the Aire gene into DCs by using a virus vector adeno-associated virus (AAV) or a lentivirus; 3, over-expressing Aire protein by the modified DCs, so that the modified DCs can express a plurality of autoantigens; and step 4, injecting the DCs subjected to Aire overexpression back into the body of the patient, and inducing immune tolerance. The preparation method has the beneficial effects that the immune regulation function of DCs is enhanced through the Aire gene, the activity of autoreactive T cells is inhibited, and the damage to pancreatic beta cells is reduced; dependence on a traditional immunosuppressor is reduced, and side effects are reduced; a novel treatment method is provided for autoimmune diseases such as type 1 diabetes, and high safety and specificity are achieved.

The invention discloses application of a soybean GmDsPTP12 gene in regulation and control of aluminum toxicity stress tolerance of plants, a nucleotide sequence of a CDS open reading frame of the GmDsPTP12 gene is as shown in SEQ ID NO: 1, and an amino acid sequence of a protein coded by the GmDsPTP12 gene is as shown in SEQ ID NO: 2. It is found that the soybean GmDsPTP12 gene can regulate and control the aluminum toxicity tolerance of soybeans, the aluminum toxicity tolerance of the soybeans is enhanced by overexpressing the soybean GmDsPTP12 gene, and the aluminum toxicity tolerance of the soybeans can be reduced by knocking out or interfering the expression of the soybean GmDsPTP12 gene.

The invention discloses a construction method and application of an ectoine-producing genetically engineered bacterium, and belongs to the field of biosynthesis. Escherichia coli BL21 is used as an original strain, lacI genes of the Escherichia coli BL21 are knocked out, an expression system regulated and controlled by tryptophan is constructed, and synthesis of a product ectoine can be regulated and controlled under the condition that an inducer is not added. According to the invention, a phosphotransferase system of Escherichia coli BL21 is modified, and a glucose readifier protein coding gene Zmglf is integrated at a ptsG gene locus, so that the inhibition effect of carbon catabolism is relieved, and the cost of a culture medium is reduced. Through overexpression of a phosphoenolpyruvate carboxylase coding gene ppc and an aspartate semialdehyde dehydrogenase coding gene asd, the accumulation of precursor substances oxaloacetic acid and L-aspartate-beta-semialdehyde in the growth and metabolism process is enhanced, and the synthesis level of the product ectoine is improved.

The invention is applicable to the technical field of biology, and provides application of a Mup3 recombinant protein in treatment of type 2 diabetes mellitus, and the amino acid sequence of the Mup3 recombinant protein is shown as SEQ ID NO.1 in a sequence table; the Mup3 recombinant protein provided by the invention is small in side effect, and the mechanism of the Mup3 recombinant protein playing a role is to directly inhibit the gluconeogenesis gene and enhance the insulin sensitivity, so that the hypoglycemic effect is obvious. The compound can play a role in treatment of type 2 diabetes mellitus, and can be further applied to prevention and treatment of other related diseases by deep research on enhancement of an insulin signal channel.

The present invention discloses a construction method and application of a genetically engineered bacterium producing ectoine, and belongs to the field of biosynthesis. The present invention uses Escherichia coli BL21 as a starting strain, knocks out the lacI Gene, construct tryptophan-regulated expression system, can regulate the synthesis of product ectoine without adding inducer. The present invention transforms the phosphotransferase system of Escherichia coli BL21. ptsG Glucose facilitator protein encoding gene Zmglf , relieving the inhibitory effect of carbon catabolism and reducing the cost of culture medium. ppc and aspartate semialdehyde dehydrogenase encoding genes asd , which enhanced the accumulation of precursor substances oxaloacetate and L-aspartate-β-semialdehyde during growth metabolism and increased the synthesis level of the product ectoine.

The invention relates to a Rhodococcus ruber SD3 locen-2 protease-like protein 2 gene enhanced strain, which is a recombinant strain obtained by improving the expression of a gene s2plp2 of R.ruber SD3 in a R.ruber SD3 wild strain. The Rhodococcus ruber SD3 locen-2 protease-like protein 2 gene enhanced strain has the advantages that the expression of the gene s2plp2 of the R.ruber SD3 is improved; the method for constructing the enhanced strain comprises the following steps: carrying out PCR (Polymerase Chain Reaction) amplification on a gene s2plp2 of R.ruber SD3, and constructing a recombinant plasmid pNV18-s2plp2 carrying the gene s2plp2; the method comprises the following steps: carrying out recombinant escherichia coli expression on recombinant escherichia coli to obtain plasmids, transforming the plasmids into E. coli Top 10 competent cells, culturing a large amount of recombinant escherichia coli, extracting the plasmids, and electrically transforming the extracted plasmids into R.ruber SD3 competent cells to obtain the R.ruber s2plp2 gene enhanced strain R.ruber s2plp2-E. ruber s2plp2-E. ruber s2plp2-E. ruber s2plp2-E. ruber s2plp2-E. ruber s2plp2-E. ruber s2plp2. Compared with an R.ruber SD3 wild strain, the expression quantity of the s2plp2 gene in the R.ruber SD3 s2plp2 gene enhanced strain is obviously enhanced by 3.35 times, and the R.ruber SD3 s2plp2 gene enhanced strain shows stronger tolerance to phenol stress.

The present invention relates to methods and compositions for gene therapy of retinitis pigmentosa related to mutations in pre-mRNA processing factor 31 (PRPF31).

The present invention provides a benzoxazole small molecule compound, which is an inhibitor of the Zeste gene enhancer homolog 2 (EZH2) and can be used to prevent and / or treat EZH2-mediated related diseases, such as malignant tumors.

The invention belongs to the technical field of gene engineering, and particularly relates to a salt stress forward regulation gene BcCRK35 and application thereof. The nucleotide sequence of the BcCRK35 gene is as shown in SEQ ID NO. 1; the amino acid sequence is as shown in SEQ ID NO. 2; the gene can be used for regulating and controlling the salt tolerance of saccharomycetes and regulating and controlling the salt tolerance of non-heading Chinese cabbages. The gene is over-expressed in yeast, so that the tolerance of the yeast to salt stress is enhanced; the gene is knocked out or silenced, so that the salt tolerance of the non-heading Chinese cabbage is reduced. According to the invention, overexpression of constructed BcCRK35 yeast proves that the gene plays a positive regulation role in salt stress. Meanwhile, after the BcCRK35 gene is silenced, the tolerance of a silenced plant to salt stress is reduced. The invention lays a theoretical foundation for researching a plant salt stress response mechanism, also provides a gene resource for cultivating a new variety of salt-tolerant non-heading Chinese cabbage, and has a relatively good potential application value.

The application relates to the biotechnology field and discloses a drought stress related alfalfa gene and application thereof, and the MsCAS15 gene is cloned from alfalfa, the nucleotide sequence of which is shown as SEQ ID NO. 1, and the encoded dehydrin is shown as SEQ ID NO. 2. The protein is rich in glycine and lysine and has the functions of protecting cell membranes and biological macromolecules. The application obtains a transgenic plant overexpressing the MsCAS15 by constructing a recombinant expression vector containing the gene and using an agrobacterium-mediated method. The experimental results show that overexpression of the gene enhances the drought resistance of the plant, and the specific performance is that the seed germination rate of the transgenic plant is higher than that of the wild type under osmotic stress, and under the natural drought condition of soil, the wilting degree of the transgenic plant leaves is reduced, and the growth state is better than that of the wild type. The application provides an effective gene resource for cultivating new drought-resistant crop varieties.

The present invention relates to a preparation method for an amide compound and an application thereof in the field of medicine. Specifically, provided by the present invention is an amide small molecule compound which is an inhibitor of Zeste gene enhancer homolog 2 (EZH2) and which may be used to prevent and / or treat EZH2-mediated related diseases, comprising tumors, myeloproliferative diseases or autoimmune diseases.

The invention discloses a multiple PCR (Polymerase Chain Reaction) Panel for detecting favorable allelic variation of wheat yield related traits and application of the multiple PCR Panel. The multiplex PCR Panel takes 9 genetic synergistic sites related to wheat yield formation as targets and is used for performing targeted detection on corresponding favorable allelic variation. The nucleotide sequences of the primer group are as shown in SEQ ID No. 21 to SEQ ID No. 38. The PCR Panel can simultaneously and accurately analyze excellent allelic variation of a grain weight synergistic site, a grain width synergistic site and a plant height related site in a single reaction, and compared with a traditional marker detection mode taking a single gene or a single character as a target, the PCR Panel has the advantage that the analysis efficiency of multi-site genetic information can be remarkably improved. The multiple PCR Panel system is embedded into a conventional wheat breeding process, a cooperative breeding mode of'precise complementary matching of parents, primary screening of low-generation basic agriculture and targeted molecular selection of middle-generation and high-generation 'is constructed, rapid selection, polymerization and fixation of multiple sites of yield-related traits are realized, the breeding period is shortened, and the new germplasm creation efficiency is improved.

The invention discloses a mitochondrial transcriptional regulatory factor Mt-CREB and application thereof in screening and preparing a drug for resisting brain aging. According to the present invention, based on the characteristic that the mitochondrial circular DNA has the conservative cAMP reaction element sequence CRE, the CREBWT capable of selectively identifying and combining the CRE or the amino acid RRPSYR mutant CREBDIEDML of the CREBWT at the 130-135th site is adopted as the key element of the gene enhancement regulation, and the CREBWT or the CREBDIEDML is selectively positioned to the mitochondria by using the mitochondrial targeting sequence so as to achieve the detection and the regulation of the mitochondrial gene expression enhancement; on one hand, the invention discloses an amino acid sequence of the Mt-CREBDIEDML, a nucleotide sequence for coding the Mt-CREBDIEDML, an expression vector containing the nucleotide sequence, and an application of the Mt-CREBDIEDML in preparation of a drug for resisting brain aging; on the other hand, the invention discloses a fluorescence and / or chemiluminiscence detection method for screening the anti-brain aging drugs by using the Mt-CREBWT. Through research on biochemical indexes, immunohistochemistry, gene expression level and the like, a new way is provided for preparing and screening anti-brain aging drugs and treating memory decline caused by aging.

The invention relates to the technical field of gene engineering, in particular to a Cilp gene enhancer and application thereof. Through integration and verification of an ATAC-seq map, a single cell transcriptome data set and multi-omics data, the Cilp gene enhancer is obtained through screening, and a mouse inner ear microinjection system is utilized to further prove that the Cilp gene enhancer can significantly enhance the transcriptional activity of an EGFP protein gene in cochlea type 2 neuronal cells. Compared with other sequences with similar sizes, the Cilp gene enhancer provided by the invention can drive specific expression of a reporter gene or a Cilp gene in cochlear neurons, especially type 2 neurons, and the gene expression level is remarkably improved. The Cilp gene enhancer provided by the invention is suitable for biological materials such as recombinant DNA (deoxyribonucleic acid), vectors or adenoviruses, can be used for promoting transcription of EGFP (enhanced green fluorescent protein) genes or other genes in cochlea neurons, and provides a new targeting tool for gene therapy of hereditary hearing loss.

The invention provides a method for enhancing insect resistance of a tobacco plant. The method comprises the step of reducing the expression level of an NtCYP94B3-1 gene and / or an NtCYP94B3-2 gene in tobacco. Through the NtCYP94B3-1 and NtCYP94B3-2 of a targeted jasmonic acid signal pathway, single-gene or multi-gene inhibition expression or knockout is carried out in a plant, and it is found that the insect resistance, especially the resistance to prodenia litura and tobacco hornworm, of the knockout plant can be greatly enhanced; meanwhile, after the gene is knocked out, the growth of tobacco and the yield and quality of flue-cured tobacco leaves are not influenced, and the gene has a wide application prospect in the aspects of molecular breeding and the like.