52 results about "Genetic enhancement" patented technology

The present invention relates to compositions and methods to modulate alpha-secretase and / or to improve cognitive ability. The invention further relates the improved / enhanced cognitive ability in diseased individuals, particularly Alzheimer's Disease patients, and treatment thereof through increased sAPP production. Macrocyclic lactones (i.e. bryostatin class and neristatin class) are compounds preferred for use with the present composition. The present invention also provides methods for increasing the generation of non-amyloidogenic soluble APP comprising the activation of protein kinase C (PKC) by administering an effective amount of PKC activator(s).

The invention provides high-nucleoside-yield bacillus subtilis engineering bacterium as well as a construction method and application thereof The invention also provides a method for producing nucleoside by fermentation. The method comprises the following steps of (1) enhancing a glutamate synthase gene gltA for encoding an NCBI reference sequence WP-009967365.1 on a bacillus subtilis chromosome;and / or enhancing a glutamine synthetase gene glnA for encoding an NCBI reference sequence WP-003231737.1 on the chromosome of the bacillus subtilis; and (2) applying the gene-enhanced strain obtainedin the step (1) to fermentation production of nucleoside. The bacillus subtilis engineering bacterium provided by the invention is a high-nucleoside-yield strain, can effectively accumulate nucleoside, improves the yield of nucleoside, and lays a foundation for industrial production of nucleoside.

The invention relates to an expression vector comprising the promoter of the mCMV-IE2 gene, or a functional expression promoting fragment thereof, and / or an enhancer of the mCMV-IE2 gene, or a functional expression enhancing fragment thereof, wherein the expression vector does not contain any complete gene of the mCMV.

The invention discloses a method for enhancing secretion of glucose oxidase by coexpression of UPR (unfolded protein response) key genes and downstream target genes, and belongs to the technical field of genetic engineering. The method comprises the steps of respectively cloning and connecting pichia pastoris UPR key gene Haclp and the downstream target genes Pdi1, Erol, Lhs1, Kar2 and Sil1 to pichia pastoris expression carriers pPICZ alpha and pPIC3.5K, transforming into a Pichia pastoris GS115-pPIC9K-GOD strain of which the preservation number is CCTCC NO: M 2012266, and obtaining a strain with enhanced secretion expression of the glucose oxidase compared with the original strain by screening and identifying. The enzyme activity of the glucose oxidase expressed by the strain on a 3L of fermentation tank is 585U / mL. Therefore, good foundation is established for large-scale production of the glucose oxidase.

The invention provides gRNA for knocking out BCL11A genes or BCL11A gene enhancers, a gRNA composition, an expression vector, CRISPR-Cas9 RNP, a CRISPR-Cas9 RNP composition, a CRISPR-Cas9 system, an electrorotation method, a reagent kit and applications thereof. A CRISPR-Cas9 technique is adopted to perform targeted cutting on hemopoietic stem cells, so that the BCL11A gene expression amount is reduced, and the hemochrome expression quantity of fetuses is high. The technique is hopeful to become a new means for treating thalassaemia and sickle cell anemia. The CRISPR-Cas9 technique is adoptedto realize mutation of BCL11A genes. The design is simple, the use is convenient, the cost is low, and the efficiency is high.

The invention discloses a gene GmWRKY148 capable of improving resistance to phytophthora sojae and application of the gene, wherein the gene can enhance resistance of soybean to the phytophthora sojae. According to the disclosed gene GmWRKY148, the nucleotide sequence of the gene is shown as SEQ ID NO.1. Phenotype and phytophthora accumulation indexes of soybeans Williams with GmWRKY148 overexpression under the phytophthora stress condition indicate that the GmWRKY148 plays an important regulatory role in improving plant resistance to phytophthora. The gene is used for genetic engineering improvement of plant resistance to phytophthora root rot, it is found that the gene has the certain effect on breeding plant varieties with high resistance to phytophthora root rot, and by improving plantresistance to the phytophthora, the plant yield and quality service are improved.

One embodiment of the invention is directed to a genetically enhanced cyanobacterium for the production of a first chemical compound, comprising at least one first recombinant gene encoding a first biocatalyst for the production of the first chemical compound, wherein the gene is under the transcriptional control of a Co2+ or Zn2+-inducible promoter. Such a cyanobacterium can provide a tighter control of the production of the first chemical compound.

The present invention discloses process of constructing transforming growth factor beta2 gene modified tissue engineering cartilage. The process includes the separation, culturing and proliferation of adult stem cell from human fat, the transfection of type 5 copying defect adenovirus to the adult stem cell from human fat, the compound of the transfected cell and the polyhydroxy acetic acid / calcium alginate composite as the carrier material, and the formation of in vivo tissue engineering cartilage. Experiment shows that it is possible to form in vivo tissue engineering cartilage through inoculating adult stem cell from human fat to the polyhydroxy acetic acid / calcium alginate composite, and this shows the application foreground of gene reinforced tissue engineering in repairing cartilage defect.

The invention discloses an anti-EpCAM chimeric antigen receptor encoding gene, a preparation method, plasmid and immune cells with the gene and application of the encoding gene. The encoding gene at least comprises an EpCAM bond zone and a CTLA4 bond zone, and comprises a T2A nucleic acid artificial sequence between the two bond zones as well; the anti-EpCAM chimeric antigen receptor encoding genealso comprises a transmembrane-stimulation structure domain; and the transmembrane-stimulation structure domain is selected from all or partial segments of CD8, CD27, CD28, CD226, 4-1BB, OX40 and ICOS molecules. By adopting the anti-EpCAM chimeric antigen receptor encoding gene disclosed by the invention, the killing effects of T cells upon tumor cells can be improved.

The invention relates to application of an AtNRT1.8 gene to strengthening the resistance of crops to the stress of heavy metals or salts, in particular to application of an AtNRT1.8 gene or encoded protein or polypeptide thereof to strengthening the resistance of plants to the stress of heavy metals, salts or draught. The invention also provides a genetic engineering method for strengthening the resistance of plants to the stress of heavy metals, salts or draught by utilizing the AtNRT1.8 gene, a vector and a host cell containing the AtNRT1.8 gene, a method for preparing transgenosis plants and the transgenosis plants. The method can strength the resistance of the plants to the stress of the heavy metals, the salts and the draught and has active effects for improving the yield and properties of crops and wide application potential.

The invention discloses a Liriodendron hybrids LhMKK2 gene, expression protein of the Liriodendron hybrids LhMKK2 gene and application of the liriodendron hybrids LhMKK2 gene. The nucleotide sequence is showed in SEQID NO.1 and the amino acid sequence is showed in SEQID NO.2. On the basis of the completely established somatic embryogenesis system of the Liriodendron hybrids and the fact that part of the transcriptome data is already obtained, the full length of the Liriodendron hybrids LhMKK2 gene, through the adoption of homology-based cloning, is obtained and is named as LhMKK2. Through gene expression analysis, that the Liriodendron hybrids LhMKK2 gene is widely expressed in plants and is involved in the growth and development of plants, and the adversity stress response process are proved; through functional analysis of the gene, it is discovered that the overexpression LhMKK2 gene is capable of enhancing resistance of plants to salt stress response, influencing the growth and development of plants and providing theoretical basis for stress resistance molecular breeding of Liriodendron hybrids and can be further widely used in adversity resistance stress of plants.

The invention relates to a myogenin (MyoG) gene enhancer, belonging to the technical field of genetic engineering. The MyoG gene enhancer consists of regulatory elements, namely E-box, SRE, KLF3, E-box, Sp1, E-box, Sp1 and TEF-1. The MyoG gene enhancer is characterized in that the sequence of the regulatory elements of the MyoG gene enhancer is E-box, SRE, KLF3, E-box, Sp1, E-box, Sp1 and TEF-1; and the SE nucleic acid sequence of the MyoG gene enhancer is shown in Seq ID No: 1 and has the length of 147 bp. Due to an artificially-synthesized enhancer sequence, the expression of a MyoG gene can be promoted at the proliferation and differentiation stages of myoblasts, and the expression activity of the MyoG gene can be remarkably improved.

A novel method to enhance translocation of molecules across or into cellular membranes using a plastid protein transport gene is described. The method can also be used to incorporate substances into membranes of organisms. Nucleic acid constructs include those which express a plastid protein transport protein or its equivalent in cells of all organisms.

The present invention relates to a method for enhancing the environmental stress resistance of plants using an environmental stress resistance-controlling gene. More specifically, the invention relates to a method for enhancing the environmental stress resistance of plants and a method for producing environmental stress-resistant plants, each of the methods comprising introducing into the plants an environmental stress resistance-controlling gene derived from Arabidopsis thaliana, as well as environmental stress-resistant plants produced by the method.

The invention discloses a ginseng amino acid transport protein gene PgLHT and an encoding protein and an application thereof. The amino acid transport related gene PgLHT has a sequence as shown in SEQ ID NO.1, and the encoding protein has the sequence as shown in SEQ ID NO.2. Researches find that PgLHT genetic expression has the tissue specificity and is closely related to ginseng stress response. The PgLHT gene has obvious effect in enhancing the ginseng plant resistance and the like. Ginseng and other plants can be screened or improved through the gene or derivatives thereof, so that excellent plant varieties with strong environment adaptivity can be obtained. The organic nutrition metabolism of plants of ginseng and the like can also be promoted through adopting a tissue culture or transgene mode.

The invention discloses a coding gene of an anti-B7-H3 chimeric antigen receptor, a preparation method, a plasmid with the gene, an immune cell and an application thereof, and the coding gene of the anti-B7-H3 chimeric antigen receptor is characterized by at least comprising an artificial nucleotide sequence for expressing IL15 and CCL21. The coding gene of the anti-B7-H3 chimeric antigen receptorprovided by the invention enhances the killing effect of CIK cells on solid tumor cells.

The invention belongs to the field of biological information, and discloses a cancer classification and characteristic gene selection method, which comprises the following steps of: establishment of a primary learner: establishing T logistic regression models and a spark group lasso regularized loss function solving model corresponding to the T logistic regression models, and outputting a secondary learner training set; establishing a secondary learner: establishing a multi-response regression model and a loss function solving model corresponding to L1 regularization, and outputting a training set prediction result; and a prognosis feature selection model: establishing a prognosis feature selection SGL model. According to the cancer classification and feature gene selection method, the three standards of prediction, stabilization and selection are met, the accuracy and stability of the model on cancer classification prediction are improved through stacking integration, oncogenes and cancer-related genes are accurately selected, and the interpretability of the model is enhanced; gene and gene pathway priori knowledge are fused, and the accuracy of cancer classification and the effectiveness of feature selection are improved.

The present invention discloses application of an antigen causing antibody-dependent enhancement of Tembusu virus. The antigen causing the antibody-dependent enhancement of Tembusu virus has an aminoacid sequence shown in SEQ ID NO.1. The antigen causing the antibody-dependent enhancement of the Tembusu virus is applied to preparation of a Tembusu virus vaccine. According to the invention, the epitope protein expressed by escherichia coli is used for immunizing ducklings, so that high-level specific antibodies can be induced in the ducklings, and the antibodies can promote virus proliferationand increase the titer of the virus in the ducklings. In the future research and development of the Tembusu virus vaccine, this kind of antigen can be removed through molecular design to avoid the antibody-dependent enhancement caused by the vaccine and ensure the protection effect. Therefore, identifying the antigen causing the antibody-dependent enhancement of the Tembusu virus has an importantapplication value in developing effective and safe vaccines and preventing and controlling the epidemic of the Tembusu virus.

The invention relates to a genetically engineered bacterium for producing gibberellin GA3 at high yield, a construction method and application. According to the present invention, by enhancing the gene expressing the nitrogen source regulatory factor AreA and the gene expressing the global regulatory factor Lae1 in the gibberella zeylanica, the positive regulation effect of the gibberella zeylanica on the related gene in the gibberellin anabolism path is enhanced, the transcriptional level of the related gene is improved, and the accumulation of gibberellin GA3 is improved. According to the recombinant gibberellin GA3 high-yield recombinant gibberellin GA3 provided by the invention, compared with wild gibberellin GA3, the transformed recombinant gibberellin GA3 has the advantages that the overall transcriptional level of a GA gene cluster is enhanced, the synthesis capability of a metabolic process is improved, the transcriptional inhibition of GA3 synthesis related genes is weakened, a carbon source substance and a nitrogen source substance can be better utilized for producing gibberellin GA3, and the yield of gibberellin GA3 is improved. The level of the transformed strain with the optimal performance for producing gibberellin GA3 through fermentation is improved from 2 g / L to 3.25 g / L compared with the original strain, the production capacity of gibberellin GA3 is remarkably improved, and the strain can be applied to large-scale production of gibberellin GA3.

The invention discloses a mouse Down's syndrome key protein screening method based on a gene-enhanced skeleton particle swarm optimization feature selection algorithm, which comprises the following steps: randomly dividing an original data set into two parts, namely a training set and a test set, for original mouse cerebral cortex protein detection, which are used for optimizing a particle swarm and testing a final result; carrying out dimension extraction on the data set to initialize a population to obtain an original population; calculating the fitness of each dimension of gene in the initial population, determining whether the gene of the dimension is selected or not, calculating the accuracy of particle prediction according to the selected gene, and identifying the key category protein for detecting the Down's syndrome of the mouse. In the aspect of particle swarm optimization, compared with a traditional mouse Down's syndrome key protein screening method, the method can quickly and efficiently identify a small number of key feature subsets with good classification performance in an original data set.

The invention discloses engineering bacteria for producing L-rhamnose, and a construction method and application of the engineering bacteria. Genes associated with synthesis of the L-rhamnose are over-expressed in escherichia coli, a synthesis pathway of the L-rhamnose is enhanced, meanwhile, through blocking of a bypass path, the accumulation amount of a precursor L-lactic aldehyde is increased,a set of efficient whole-cell catalytic synthesis method of free L-rhamnose is developed, and the L-rhamnose is synthesized firstly in the escherichia coli by taking glucose as a single substrate. Inthis way, the cheap glucose can serve as raw materials, the L-rhamnose is efficiently synthesized through whole-cell catalysis of the escherichia coli, the synthesized rhamnose is in a free state, thecomplex hydrolysis step is avoided, the synthesized rhamnose can be directly used for separation purification, an obtained product does not contain other monosaccharides or proteins either, and thusthe burden of subsequent separation purification is reduced.

The invention provides application of gene enhanced immune cells in lung cancer, and belongs to the technical field of immunology. The gene enhanced immune cells provided by the invention are NK (Natural Killer) cells transfected with siRNA (Small Interference Ribonucleic Acid) of lnc-AL592494.1. The NK cells provided by the invention have stronger multiplication capacity, and have stronger killing activity on lung cancer cells.

The invention belongs to the technical field of agricultural biology and particularly relates to application of a gene BEC1019 in improvement of full rot resistance of wheat. A method for improving the full rot resistance of wheat comprises a step of silencing the gene BEC1019 in the wheat. The gene BEC1019 is an important effector gene in full rot of the wheat, the gene can be used for enhancingresistance of the wheat to multiple pathogenic bacteria, and new wheat species with durable and broad spectrum resistance can be bred.