49 results about "Genetic enhancement" patented technology

The present invention relates to compositions and methods to modulate alpha-secretase and/or to improve cognitive ability. The invention further relates the improved/enhanced cognitive ability in diseased individuals, particularly Alzheimer's Disease patients, and treatment thereof through increased sAPP production. Macrocyclic lactones (i.e. bryostatin class and neristatin class) are compounds preferred for use with the present composition. The present invention also provides methods for increasing the generation of non-amyloidogenic soluble APP comprising the activation of protein kinase C (PKC) by administering an effective amount of PKC activator(s).

The invention discloses a method for enhancing secretion of glucose oxidase by coexpression of UPR (unfolded protein response) key genes and downstream target genes, and belongs to the technical field of genetic engineering. The method comprises the steps of respectively cloning and connecting pichia pastoris UPR key gene Haclp and the downstream target genes Pdi1, Erol, Lhs1, Kar2 and Sil1 to pichia pastoris expression carriers pPICZ alpha and pPIC3.5K, transforming into a Pichia pastoris GS115-pPIC9K-GOD strain of which the preservation number is CCTCC NO: M 2012266, and obtaining a strain with enhanced secretion expression of the glucose oxidase compared with the original strain by screening and identifying. The enzyme activity of the glucose oxidase expressed by the strain on a 3L of fermentation tank is 585U/mL. Therefore, good foundation is established for large-scale production of the glucose oxidase.

The invention discloses a gene GmWRKY148 capable of improving resistance to phytophthora sojae and application of the gene, wherein the gene can enhance resistance of soybean to the phytophthora sojae. According to the disclosed gene GmWRKY148, the nucleotide sequence of the gene is shown as SEQ ID NO.1. Phenotype and phytophthora accumulation indexes of soybeans Williams with GmWRKY148 overexpression under the phytophthora stress condition indicate that the GmWRKY148 plays an important regulatory role in improving plant resistance to phytophthora. The gene is used for genetic engineering improvement of plant resistance to phytophthora root rot, it is found that the gene has the certain effect on breeding plant varieties with high resistance to phytophthora root rot, and by improving plantresistance to the phytophthora, the plant yield and quality service are improved.

One embodiment of the invention is directed to a genetically enhanced cyanobacterium for the production of a first chemical compound, comprising at least one first recombinant gene encoding a first biocatalyst for the production of the first chemical compound, wherein the gene is under the transcriptional control of a Co²⁺ or Zn²⁺-inducible promoter. Such a cyanobacterium can provide a tighter control of the production of the first chemical compound.

The invention discloses a Liriodendron hybrids LhMKK2 gene, expression protein of the Liriodendron hybrids LhMKK2 gene and application of the liriodendron hybrids LhMKK2 gene. The nucleotide sequence is showed in SEQID NO.1 and the amino acid sequence is showed in SEQID NO.2. On the basis of the completely established somatic embryogenesis system of the Liriodendron hybrids and the fact that part of the transcriptome data is already obtained, the full length of the Liriodendron hybrids LhMKK2 gene, through the adoption of homology-based cloning, is obtained and is named as LhMKK2. Through gene expression analysis, that the Liriodendron hybrids LhMKK2 gene is widely expressed in plants and is involved in the growth and development of plants, and the adversity stress response process are proved; through functional analysis of the gene, it is discovered that the overexpression LhMKK2 gene is capable of enhancing resistance of plants to salt stress response, influencing the growth and development of plants and providing theoretical basis for stress resistance molecular breeding of Liriodendron hybrids and can be further widely used in adversity resistance stress of plants.

A novel method to enhance translocation of molecules across or into cellular membranes using a plastid protein transport gene is described. The method can also be used to incorporate substances into membranes of organisms. Nucleic acid constructs include those which express a plastid protein transport protein or its equivalent in cells of all organisms.

The present invention relates to a method for enhancing the environmental stress resistance of plants using an environmental stress resistance-controlling gene. More specifically, the invention relates to a method for enhancing the environmental stress resistance of plants and a method for producing environmental stress-resistant plants, each of the methods comprising introducing into the plants an environmental stress resistance-controlling gene derived from Arabidopsis thaliana, as well as environmental stress-resistant plants produced by the method.

The invention discloses a coding gene of an anti-B7-H3 chimeric antigen receptor, a preparation method, a plasmid with the gene, an immune cell and an application thereof, and the coding gene of the anti-B7-H3 chimeric antigen receptor is characterized by at least comprising an artificial nucleotide sequence for expressing IL15 and CCL21. The coding gene of the anti-B7-H3 chimeric antigen receptorprovided by the invention enhances the killing effect of CIK cells on solid tumor cells.

The invention belongs to the field of biological information, and discloses a cancer classification and characteristic gene selection method, which comprises the following steps of: establishment of a primary learner: establishing T logistic regression models and a spark group lasso regularized loss function solving model corresponding to the T logistic regression models, and outputting a secondary learner training set; establishing a secondary learner: establishing a multi-response regression model and a loss function solving model corresponding to L1 regularization, and outputting a training set prediction result; and a prognosis feature selection model: establishing a prognosis feature selection SGL model. According to the cancer classification and feature gene selection method, the three standards of prediction, stabilization and selection are met, the accuracy and stability of the model on cancer classification prediction are improved through stacking integration, oncogenes and cancer-related genes are accurately selected, and the interpretability of the model is enhanced; gene and gene pathway priori knowledge are fused, and the accuracy of cancer classification and the effectiveness of feature selection are improved.

The invention relates to a genetically engineered bacterium for producing gibberellin GA3 at high yield, a construction method and application. According to the present invention, by enhancing the gene expressing the nitrogen source regulatory factor AreA and the gene expressing the global regulatory factor Lae1 in the gibberella zeylanica, the positive regulation effect of the gibberella zeylanica on the related gene in the gibberellin anabolism path is enhanced, the transcriptional level of the related gene is improved, and the accumulation of gibberellin GA3 is improved. According to the recombinant gibberellin GA3 high-yield recombinant gibberellin GA3 provided by the invention, compared with wild gibberellin GA3, the transformed recombinant gibberellin GA3 has the advantages that the overall transcriptional level of a GA gene cluster is enhanced, the synthesis capability of a metabolic process is improved, the transcriptional inhibition of GA3 synthesis related genes is weakened, a carbon source substance and a nitrogen source substance can be better utilized for producing gibberellin GA3, and the yield of gibberellin GA3 is improved. The level of the transformed strain with the optimal performance for producing gibberellin GA3 through fermentation is improved from 2 g/L to 3.25 g/L compared with the original strain, the production capacity of gibberellin GA3 is remarkably improved, and the strain can be applied to large-scale production of gibberellin GA3.

The invention provides application of gene enhanced immune cells in lung cancer, and belongs to the technical field of immunology. The gene enhanced immune cells provided by the invention are NK (Natural Killer) cells transfected with siRNA (Small Interference Ribonucleic Acid) of lnc-AL592494.1. The NK cells provided by the invention have stronger multiplication capacity, and have stronger killing activity on lung cancer cells.

The invention belongs to the technical field of agricultural biology and particularly relates to application of a gene BEC1019 in improvement of full rot resistance of wheat. A method for improving the full rot resistance of wheat comprises a step of silencing the gene BEC1019 in the wheat. The gene BEC1019 is an important effector gene in full rot of the wheat, the gene can be used for enhancingresistance of the wheat to multiple pathogenic bacteria, and new wheat species with durable and broad spectrum resistance can be bred.

The present invention belongs to the field of agricultural biotechnology and particularly relates to an application of gene BE1019 in improving resistance to powdery mildew of wheat. The method for improving the resistance to the powdery mildew of the wheat comprises a step of silencing the gene BE1019 in the wheat. The BEC1019 gene is an important effector gene of wheat powdery mildew, can be used to enhance resistance of wheat to a variety of pathogenic micro-organisms and cultivates new wheat varieties with durable and broad-spectrum resistance.