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Enhancement of stress tolerance in plants

A technology of plant cells and transgenic plants, which is applied in the field of gene expression changes and can solve problems such as improving tolerance

Inactive Publication Date: 2010-02-10
ARBORGEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, along with improved tolerance to cold, drought and salt load, expression of Arabidopsis CBF1 in transgenic plants was accompanied by negative effects on plant growth and yield under normal growing conditions

Method used

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  • Enhancement of stress tolerance in plants
  • Enhancement of stress tolerance in plants
  • Enhancement of stress tolerance in plants

Examples

Experimental program
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Effect test

example 1

[0113] Preparation of DNA constructs containing the Arabidopsis CBF2 gene

[0114] Plasmid pMEN068 containing the Arabidopsis CBF2 (AtCBF2) gene (GenBank Accession No. AF074601) and the Arabidopsis rd29A promoter driving AtCBF2 expression was obtained from Mendel Biotechnology, Inc. The rd29A::CBF2::E9ter fragment from the pMEN68 plasmid was cloned into the ArborGen backbone pWVR5. The backbone vector pWVR5 is the pBI121 vector with the 35S promoter GUS sequence removed and the NOS promoter replaced by the UBQ10 promoter from Arabidopsis (Clontech laboratories, Palo Alto, CA (Palo Alto CA)) (Sun, C.W and Callis, J (1997) Plant J., 11:101-111). Next, the pABTV14 plasmid was obtained. The rd29A::CBF2::E9ter sequence was then digested with Kpn I / / Pst I from the pABTV14 plasmid and subcloned into the pAGF243 plasmid (US Patent Application No. 10 / 946622) to obtain the pABCTE01 plasmid ( Figure 1A, SEQ ID NO: 12) with the ArborGen pollen control cassette PRMC2::barnase H102E (US ...

example 2

[0116] Transgenic Arabidopsis plants expressing the CBF2 gene have enhanced freezing tolerance

[0117] Arabidopsis plants were transformed with plasmid pMEN068 containing the rd29A promoter and the AtCBF2 gene using vacuum invasion as described by P. J. Green P. J. Plant Physiol. 119:331-342 (1999). T2 seeds of transgenic Arabidopsis plants were germinated on Petri dishes on agar containing 1×MS salts and 0.5% sucrose plus kanamycin (35 μg / ml) at room temperature. In the case of germination of wild-type control Arabidopsis seeds, no kanamycin was added to the agar. After 21 days of growth at 22°C under constant light, new plant seedlings grown on agar in petri dishes were grown in the light at 4°C to induce the rd29A promoter, and then exposed to -10°C for a period of time in the dark. 8 hours. After freezing stress, the plants were transferred to a growth chamber (22°C) to allow them to recover. After 2 days of recovery at 22°C, the number of plants surviving the freezing...

example 3

[0125] Transformation of Eucalyptus IPB1 clone

[0126] Eucalyptus IPB1 clones were transformed with plasmids pABCTE01 (SEQ ID NO: 12) and pABCTE03B (SEQ ID NO: 13) according to the protocol described in US Patent Application No. 10 / 981742.

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Abstract

Novel dehydrin promoters isolated from Eucalyptus dunnii and Eucalyptus macarthurii are cold-inducible and can be used for driving CBF genes in plants, including trees, to enhance tolerance to freezing temperatures or water stress and reduce undesirable effects associated with CBF gene expression.

Description

technical field [0001] The present invention relates to plant biotechnology and alteration of gene expression in transformed plants. More specifically, the present invention relates to enhancing the stress tolerance of industrially concerned plants by regulating the expression of genes encoding C-repeat-binding factors (CBF), while reducing the methods that require undesired effects associated with gene expression. [0002] This application claims priority to US Provisional Application 60 / 908,940, filed March 29, 2007. Background technique [0003] Low temperature stress is a major environmental factor that not only limits the area where plants can grow but also affects the quantity and quality of crops. Worldwide, crop yield losses due to cold damage amount to approximately $2 billion per year. In Florida, occasional freezes have forced the citrus belt to migrate further south, while in California, citrus crops have at times suffered severe damage from winter freezes in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29A01H5/00C12N15/82
CPCC07K14/415C12N15/8273C12N15/8237
Inventor 张春生金伯利·安·温克勒萨曼莎·阿比盖尔·米勒特雷莎·巴拉斯柯克·福茨赵垣玛丽昂·伍德
Owner ARBORGEN
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