63results about How to "Increased risk of contamination" patented technology

The present invention provides a cell culture data and cell image capturing and remote monitoring system that includes an imaging sensor pod having one or more cameras to capture multiple color still and/or motion images of cell cultures and cells located on a support and/or in a liquid located in a culture vessel within an incubator. The images can be taken at varying magnifications, and from different discrete locations on the support or in the vessel round the clock. The captured data and images are wirelessly transmitted to a management control unit, and/or another wirelessly connected and remotely located data transmission device such as PDA, for a researcher to review and analyze to determine the health and viability of cells, and the status of the cell culture. The image capturing and remote monitoring system taught herein alleviates the need for a researcher to be in the physical presence of a biological sample in order to visually ascertain and analyze the health and viability of the sample and status of the cell culture.

A monolithic sputtering target assembly having a one piece assembly made from the same material is disclosed. Also disclosed are other sputtering target assemblies which have a backing plate and a sputtering target blank wherein the backing plate is made from or contains a metal, such as a valve metal, cobalt, titanium, or alloys thereof. Methods of recycling target assemblies are further disclosed as well as unique methods of providing target assemblies to fabricators.

A fluidized bed process for the production of polycrystalline silicon granules supplies, in addition to reaction gas, a gas containing 99.5 to 95 mol. percent hydrogen and 0.5 to 5 mol. percent gaseous silicon compounds, and the reactor wall is maintained at the same or a higher temperature than the reaction zone, such that the deposition of silicon on reactor internals is minimized.

A device having a squeezable hollow member connected to a medication chamber in turn connected to a hollow tube fitted with an absorbent tip. The device may be used to conveniently administer a medication to an inflamed tissue. A method of providing a medication to an inflamed tissue is also provided.

The invention discloses a method for jointly stabilizing fly ash and an arsenic-containing waste and relates to a treatment process for jointly stabilizing two hazardous wastes. The method comprises the following steps of: sequentially adding the fly ash and the arsenic-containing waste into a kettle type reactor according to a dry basis mass ratio of 1:(20-40); then adding a regulator solution being 10-40 percent of the weight of solid; and mechanically stirring and reacting for 5-30 minutes at normal temperature. After reacting, the volume of the mixed wastes is reduced by 10-30 percent, and the stabilization features of the product are greatly improved. Arsenic in the arsenic-containing waste is stabilized by the utilizing the physical and chemical characteristics of small grain diameter, large specific surface area, high Ca content, strong alkalinity, gel activity, and the like of the fly ash, and meanwhile, the migratory capacities of heavy metals such as Pb, Se, Cd, Ni, and the like in the fly ash are also obviously reduced and conform to an arrival standard of a hazardous waste landfill site. The method treats wastes by using wastes, greatly reduces the treatment cost, has simple equipment and considerable economic, social and environmental benefits and can be widely popularized and applied to the treatment of the fly ash and the arsenic-containing waste so as to achieve the purpose of stabilization.

The invention relates to methods of isolating nucleic acids from a sample using a filter device comprising a plurality of membranes. The invention also provides for devices comprising a plurality of membranes and kits suitable for isolating nucleic acids from a sample.

The invention relates to the field of medical instrument in-vitro diagnostic reagents, in particular to a cleaning solution or diluent thereof special for a full-automatic chemiluminescence tester anda preparation method thereof. The cleaning solution comprises an inorganic salt, a phosphate buffer system, a surfactant 1, a surfactant 2, a preservative and water; the inorganic salt consists of sodium chloride and potassium chloride; the phosphate buffer system is one of a disodium hydrogen phosphate-potassium dihydrogen phosphate system and sodium dihydrogen phosphate-dipotassium hydrogen phosphate; the surfactant 1 is one of tween-20 and tween-80; the surfactant 2 is one of sodium dodecyl sulfate and sodium laurate; and the preservative is composed of Proclin300 and Bronidox. The cleaning solution provided by the invention has the characteristics of simple preparation process and low cost, is suitable for full-automatic chemiluminescence testers of different manufacturers, at the same time is suitable for detection of items with different detection principles, and has good accuracy, high precision and good corrosion resistance.

A method of cleaning a vessel having deposits on an interior surface includes removably bonding an ultrasonic transducer to an external wall of the vessel and using the ultrasonic transducer to produce ultrasonic energy coupled into the vessel wall such that at least a portion of the ultrasonic energy is transmitted to the interior surface.

A mirror for reflecting extreme ultraviolet light (EUV) comprising: a substrate layer; and an upper layer above the substrate layer, that reflects EUV wavelengths and refracts longer wavelengths, said upper layer being dense and hard carbon having an Sp2 to Sp3 carbon bond ratio of 0 to about 3 and a normal incidence EUV mirror comprising an optical coating on an uppermost surface which permits transmission of EUV and protects the surface from environmental degradation, said coating being dense and hard and having an Sp2 carbon bond ratio of 0 to about 3 and a thickness of 0.1 to about 5 nanometers. The invention also includes EUV mirror systems protected by a dense carbon layer and includes a multilayer EUV reflecting system having an out of band absorbing layer.

A composition containing a macrophage inhibitor may be administered in an effective amount to prevent, inhibit or reduce restenosis, thrombus or aneurysm formation in implanted polymeric vascular grafts. The composition may be administered prior to vascular graft implantation, at the same time as vascular graft implantation, following vascular graft implantation, or any combination thereof. Examplary macrophage inhibitors include bisphosphonates, anti-folate drugs and antibodies, preferably in a controlled release or liposomal formulation.

One embodiment of the invention is directed to a genetically enhanced cyanobacterium for the production of a first chemical compound, comprising at least one first recombinant gene encoding a first biocatalyst for the production of the first chemical compound, wherein the gene is under the transcriptional control of a Co²⁺ or Zn²⁺-inducible promoter. Such a cyanobacterium can provide a tighter control of the production of the first chemical compound.

The invention relates to a cell culture medium with the anti-pollution function, and belongs to the field of biotechnology. The cell culture medium disclosed by the invention aims to solve the problem that the current cell culture medium, especially a fluid culture medium cannot prevent contamination. In order to solve the problem, a method of adding 10-20 mg of ethyl ciprofloxacin to per liter of the culture medium is adopted, so that the newly prepared fluid culture medium has continuous anti-pollution capacity.

The invention relates to a method for tendering buffalo. The method comprises the following steps of: uniformly injecting exogenous enzyme liquid into buffalo according to an injection enzyme using amount of 10-80 U in 1g of buffalo and an injection volume of 0.01-0.08 mL in 1g of buffalo; standing for 3 minutes; rolling for 2 minutes; after leaching or wiping the enzyme liquid discharged to the surface, hermetically packaging with food plastic packaging bags made of polyethylene or polyvinylidene chloride; tendering for 6-8 hours under the environment of 6-12 DEG C; after tendering is finished, cooking and processing directly or storing under the condition of 0-4 DEG C for standby within 24-48 hours. According to the method, the tendering efficiency is high, the effect is obvious, and the shearing force of buffalo after being tendered is reduced by more than 45%. According to the method, influence on appearance of buffalo and product yield is slight, the freshness of the buffalo is well kept, safety and non-toxicity without bad flavor can be realized, the industrialized operability is high, and the method is suitable for tendering treatment of buffalo for cooking or deep processing.

Methods for increasing the patency of biodegradable, synthetic vascular grafts are provided. The methods include administering one or more cytokines and/or chemokines that promote outward tissue remodeling of the vascular grafts and vascular neotissue formation. The disclosed methods do not require cell seeding of the vascular grafts, thus avoiding many problems associated with cell seeding. Biodegradable, polymeric vascular grafts which provide controlled release of cytokines and/or chemokines at the site of vascular graft implantation are also provided.

Methods for treating or preventing neointima stenosis are disclosed. The methods generally involve the use of a TGFβ inhibitor, a SMAD2 inhibitor, an FGF Receptor agonist, a Let-7 agonist, or a combination thereof, to inhibit endothelial-to-mesenchymal transition (Endo-MT) of vascular endothelial cells into smooth muscle cells (SMC) at sites of endothelial damage. The disclosed methods can therefore be used to prevent or inhibit neointimal stenosis or restenosis, e.g., after angioplasty, vascular graft, or stent. Also disclosed are methods for increasing the patency of biodegradable, synthetic vascular grafts using a composition that inhibits Endo-MT. A cell-free tissue engineered vascular graft (TEVG) produced by this method is also disclosed.

The invention relates to packaging (10) for solid yeast products, such as: a supporting element (12); solid yeast products contained in one or more packages (22), supported by the supporting element (12); and an outer cover (14) retaining all of the solid yeast products contained in the packages (22) on the supporting element (12). According to the invention, all of the packages (22) located between the solid yeast products and the outer cover (14) may have a surface density no greater than 200 g/m². The invention also relates to a method for packaging solid yeast products and to a method for preserving and using solid yeast products thus packaged.

The invention provides fully-enclosed flow-stabilized and pressure-superposed energy storage water supply equipment. According to the fully-enclosed flow-stabilized and pressure-superposed energy storage water supply equipment, the problem that a common pressure-superposed water supply product has no water storage capacity or has extremely small water storage capacity is solved, and dual functionsof water storage and energy storage are introduced into a pressure-superposed water supply system; the water contention degree of a plurality of secondary water supply stations to a municipal primarywater supply pipe network is greatly reduced, the reactive power loss of water supply is greatly eliminated, the water supply efficiency is improved, and continuous, safe and high-quality water supply for users can still be realized under the condition that the flow quantity and pressure of a municipal tap water pipe network cannot meet user requirements continuously; even under the condition that the water supply of the municipal tap water pipe network is completely cut off, the water supply still can continue for a number of minutes to a number of hours; positive pressure full enclosing ofwater storage facilities can be realized, the purposes of chlorine maintaining, self cleaning and no cleaning are achieved, and the fully-enclosed sanitary safety requirement in the Chinese water supply standard is fully met; and the blank that no secondary water supply products of other forms exists between pressure-superposed water supply and water tank (pool) water storage water supply in the secondary water supply products for many years is filled up.

The invention discloses a novel PCV (porcine circovirus) mutant strain and a porcine kidney cell strain co-culturing with the novel PCV mutant strain. Cells infected by the novel PCV mutant strain have visible minimal lesions, but cell death is not caused. The sieved porcine kidney cell strain carries a PCV2, so that stable passage can be realized, a large number of viruses are generated, and the prepared viruses can be used for preventing and treating the PCV.