Cell culture medium with anti-pollution capacity

A technology of culture medium and liquid culture medium, which is applied in the field of biological science, can solve the problems of increasing user operation steps, increasing user operation difficulty, etc., and achieves the effect of reducing operation steps.

Inactive Publication Date: 2014-12-10
YUNNAN ANIMAL SCI & VETERINARY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this way, the user's operation steps will be increased, or the user's operation difficulty will be increased.

Method used

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  • Cell culture medium with anti-pollution capacity
  • Cell culture medium with anti-pollution capacity
  • Cell culture medium with anti-pollution capacity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Prepare 1 liter of MEM (Minimum Essential Medium, minimum nutrient requirements) dry powder medium: prepare MEM powder medium according to the following formula, seal it after preparation, and store it in the dark at 4°C.

[0016]

[0017]

Embodiment 2

[0019] Prepare 1 liter of 1-fold MEM: add the formula product of Example 1 into a 1 liter Erlenmeyer flask, add 900 ml of ultrapure water, mix well, and allow all ingredients to dissolve completely. The pH was adjusted to 7.0 with 1M NaOH and 1M HCl, and the volume was adjusted to 1 L with ultrapure water. Then sterilize by filtration through a 0.22 micron filter. Finally, aliquot and seal, and store at 4°C in the dark.

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Abstract

The invention relates to a cell culture medium with the anti-pollution function, and belongs to the field of biotechnology. The cell culture medium disclosed by the invention aims to solve the problem that the current cell culture medium, especially a fluid culture medium cannot prevent contamination. In order to solve the problem, a method of adding 10-20 mg of ethyl ciprofloxacin to per liter of the culture medium is adopted, so that the newly prepared fluid culture medium has continuous anti-pollution capacity.

Description

technical field [0001] The invention relates to a cell culture medium with anti-pollution ability, belonging to the technical field of biological science. Background technique [0002] Currently commercially available cell culture media are divided into two types, one is liquid culture medium and the other is dry powder culture medium. The former is packaged aseptically, but it has no anti-pollution ability; the latter is not packaged aseptically, but after preparation, it must be sterilized by filtration or autoclaved, and it does not have anti-pollution ability itself. In order to prevent contamination, users generally add antibacterial agents to prevent contamination during use, or operate as carefully as possible to avoid contamination. In this way, the user's operation steps will be increased, or the user's operation difficulty will be increased. Therefore, many users hope to have a cell culture medium that can maintain anti-pollution ability for a long time. Conten...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00
Inventor 高林李华春何于雯姚俊廖德芳朱建波
Owner YUNNAN ANIMAL SCI & VETERINARY INST
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