Product
Patsnap Eureka
For R&D, Patsnap Eureka makes reading and utilizing patents & technical documents easy.
Patsnap Eureka AIR
Designed for self-driven R&D workflows. Generate viable solutions, solve complex R&D challenges, empower your innovation with AI.
Patsnap Eureka Materials
Designed for material experts only. Revolutionize your material R&D, from search, analyze, to developing new materials.
Feature
TechResearch
Generate reliable direction feasibility study reports for your R&D in just a few steps.
TechSeek
Discover and master advanced knowledge NOW. Basics, ideas, possibilities, all at once.
TechMind
As an expert in R&D Theories, TechMind can generates customized viable solutions instantly.
TechRisk
Analyze your overall solution with one click, know your potential R&D risks in advance.
TechMonitor
Get weekly tech updates, stay abreast of the latest tech innovations and key insights.
Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
50results about How to "Short digestion time" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Detection method of total phosphorus content in water
InactiveCN106841062AReduce human errorImprove accuracyPreparing sample for investigationColor/spectral properties measurementsPotassium persulfatePhosphate
The invention provides a detection method of total phosphorus content in water. The detection method comprises the following steps: (1) determining the absorbance of a phosphate standard solution by adopting a spectrophotometric method and drawing a working curve; (2) providing a water body test sample to be detected and adding a potassium persulfate solution into the water body test sample to be detected; carrying out microwave sealed digestion; oxidizing all total phosphorus in the water body test sample to be detected into orthophosphate, so as to obtain a digestion solution; (3) adding ascorbic acid into the digestion solution and uniformly mixing for 30s to 60s; adding a molybdate solution and carrying out color development reaction to obtain a reaction solution; (4) providing a blank water sample and referring to operation of steps (2) and (3) to obtain a blank reaction solution; (5) carrying out spectrophotometric absorbance determination on the reaction solution and the blank water sample to obtain test sample absorbance and blank absorbance respectively; referring to the working curve and calculating to obtain the total phosphorus content in the water body test sample to be detected.
Owner:GUIGANG BATIAN ECOLOGY
Umbilical cord tissue mesenchymal stem cell isolated culture method
ActiveCN105238751AShort digestion timeIncrease profitSkeletal/connective tissue cellsAntibody labelingSample collection
The invention discloses an umbilical cord tissue mesenchymal stem cell isolated culture method. The method comprises the steps of A, sample collection and cell isolated culture; B, subculture, wherein after being cultured, primary cells are subcultured continuously when cell confluence reaches over 80%; C, growth curve drawing; D, cell detection, wherein the third generation of cells are adopted for detection, well digested third generation of umbilical cord mesenchymal stem cells are prepared and washed with a buffer solution, then antibody labeling is conducted on cells, incubation washing is conducted, and then detection is conducted. Two types of collagenase are used at the same time, and digestion of umbilical cord tissue is conducted with hyaluronidase and neutral protease or accutase, so that tissue blocks are quickly and fully digested.
Owner:SHENZHEN HORNETCORN BIOTECH
Forensic medical diatom inspection method based on mixed acid digestion
ActiveCN102507585AAvoid damageReduce lossesPreparing sample for investigationMaterial analysis by optical meansEpoxyFiltration
The invention discloses a forensic medical diatom inspection method based on mixed acid digestion. The forensic medical diatom inspection method comprises the steps of optical microscope inspection and scanning electron microscope inspection. The optical microscope inspection at least comprises the steps of: (1) performing a blank test; (2) performing mixed acid digestion by adding concentrated nitric acid, concentrated hydrochloric acid and a hydrogen peroxide solution in a sample to be tested and digesting in a manner of heating in water bath; (3) centrifuging and precipitating; (4) coating on a sheet and sealing by using epoxy resin; and (5) performing diatom qualitative and quantitative analysis. The scanning electron microscope inspection at least comprises the steps of: (1) performing a blank test; (2) performing mixed acid digestion by adding concentrated nitric acid, concentrated hydrochloric acid and a hydrogen peroxide solution in a sample to be tested and digesting in a manner of heating in water bath; (3) performing vacuum filtration; (4) automatically taking pictures and storing images by a scanning electron microscope; and (5) performing diatom qualitative and quantitative analysis. The forensic medical diatom inspection method based on the mixed acid digestion, disclosed by the invention, is high in sensitivity, efficient, safe, environment-friendly and exact in qualitative and quantitative analysis, and has good application prospect in the forensic medical drowning diagnosis practice.
Owner:GUANGZHOU CRIMINAL SCI & TECH RES INST
Method for measuring total content of nitrogen of fresh water quality
InactiveCN104076007AShort digestion timeThe result is accuratePreparing sample for investigationColor/spectral properties measurementsDecompositionEnvironmental chemistry
The invention provides a method for measuring the total content of nitrogen of fresh water quality. The method comprises the steps of sampling, preparing an alkaline potassium persulfate solution, preparing a potassium nitrate standard solution, preparing a hydrochloric acid solution, drawing a calibration curve and detecting a water sample to be measured. According to the method, a polytetrafluoroethylene decomposition bottle is adopted to replace a colorimetric tube, so that the decomposition process is relatively sealed and relatively fully carried out, and the decomposition time is relatively short; cross contamination is not caused between the inside and the outside of the decomposition bottle, and interference is eliminated, so that the result is relatively accurate, and the operation is relatively simple and relatively convenient; furthermore, an opening or a plug of the colorimetric tube is prevented from being broken in the decomposition process, so that the problem of analyzing by sampling again is avoided. Furthermore, after the colorimetric tube is replaced by the decomposition bottle, and a pipette is also used, the sample can be analyzed at the constant volume without being transferred into the colorimetric tube after being decomposed, so that the method is convenient, fast and easy to operate, and the accuracy is improved.
Owner:江苏省水产技术推广站
Method for extracting sub-totipotent stem cell from chorion of fetal surface of placenta
ActiveCN105200007AReduce processingReduce manpowerNervous disorderGenetic material ingredientsFiltrationMagnetic bead
The invention relates to a method for extracting sub-totipotent stem cells from a chorion of a fetal surface of a placenta. The method comprises the steps of removing an amnion and stagnated blood from the placenta, and then repetitively washing the surface of the placenta to perform sterilization; shearing the chorion of the fetal surface in a glass utensil, removing placenta lobule tissues remained on the surface as much as possible, and shearing the chorion into small blocks; washing small tissue blocks by using a screen and a great amount of normal saline, and removing residual blood cells; performing tissue digestion: performing oscillatory digestion in a constant temperature shaker by using mixed enzymes; adding a proper amount of FBS for termination after digestion, performing filtration through a filter screen, and adding a great amount of normal saline to wash filter residues to obtain cells as many as possible; performing centrifugation, abandoning supernatant, adding saline water for washing and performing centrifugation to obtain mononuclear cells; performing magnetic bead sorting (OCT-4 positive, Nanog positive and STRO-1 negative) to obtain target cells. The invention further relates to the sub-totipotent stem cells obtained by adopting the method and pharmaceutical use thereof. The sub-totipotent stem cells have excellent characteristics.
Owner:BOYALIFE
Method for digesting sample in process of measuring chemical oxygen demand (COD)
InactiveCN102109433AEasy to operateReduce acid consumptionPreparing sample for investigationTesting waterEnvironmental chemistryDigestion
The invention relates to a method for digesting a sample in the process of measuring chemical oxygen demand (COD). The method comprises the following steps of: taking 10.00ml of COD standard sample, and taking distilled water in blank; adding 5.00ml of 0.0250mol / L potassium dichromate standard solution into a water sample; respectively adding 3.3ml of 50g / L manganese sulfate solution, 1.0ml of 50g / L cerium sulfate solution and 18ml of concentrated sulfuric acid, and mixing; putting into a 160 DEG C drying box, and heating for 30 minutes; cooling to room temperature, adding 3 drops of 1,10-phenanthroline-ferrous indicator, titrating by using 0.02510mol / L ammonium ferrous sulfate, and taking a point at which the color of solution is turned from yellow to blue green to red brown as a finishing point; and recording the consumption milliliter number V1 of ammonium ferrous sulfate standard titration solution and a blank test titration consumption milliliter number V0. By the method for digesting the sample, the common drying box is used for heating, the operation process is simple and is easy to master, acid consumption is lower than that in a classical method, digestion time is short, and batch processing can be realized.
Owner:PETROCHINA CO LTD
Municipal sludge energy treatment system
ActiveCN101905945AAchieve separationHigh activityWaste based fuelBiological sludge treatmentSeparation technologyKinetic control
The invention belongs to the field of sludge treatment and relates to a municipal sludge energy treatment system. The system comprises an acidogenic reactor and a methanogenic reactor; and first-level membrane separation equipment and second-level membrane separation equipment are arranged between the acidogenic reactor and a methanogenic reactor. The sludge passes through the acidogenic reactor and is hydrolyzed to generate acid in the methanogenic reactor, and then passes through the first-level membrane separation equipment to be separated into first concentrated solution and first penetrating fluid. The first concentrated solution reflows to the acidogenic reactor, and the first penetrating fluid enters the second-level membrane separation equipment to be separated into second concentrated solution and second penetrating fluid; and the second concentrated solution enters the methanogenic reactor to be fermented to generate methane. By organically combining the two-phase anaerobic digestion technology with the membrane separation technology, the municipal sludge energy treatment system realizes the combination of a physical dialysis method and a kinetic control method and really realizes the separation of fermentative bacteria bacteria and methanogenic bacteria. Therefore, the bacteria in two phases can exert the maximum activity, and the operating stability and energy efficiency of the whole system are improved.
Owner:广州微启生物科技有限公司
Separation and purification method for islet cells of mouse
InactiveCN103013907ARich optional technologyConfiguration concentration is lowVertebrate cellsArtificial cell constructsWater bathsPurification methods
The invention belongs to the technical field of cell separation, and particularly relates to a separation method for islet cells of a mouse. The method comprises the following steps: narcotizing the mouse, disinfecting the skin and cutting along the center of the abdomen to fully expose the pancreas; shearing the ligament organization connected with the pancreas of the spleen, clamping to close the junction of the splenic vein and the spleen and cutting the heart broken, inserting a needle at the intersection of the splenic vein and the portal vein to the splenic vein, and injecting collagenase filling liquid; immersing the filled pancreas of the spleen into the collagenase filling liquid after the pancreas of the spleen is dissociated from the abdominal cavity; performing water bath slaking on the pancreas collagenase solution, performing centrifugal separation after adding Hank's liquid and taking the sediment; and performing heavy-suspending sedimentation, filtration and centrifugal treatment on the sediment to obtain the islet cells of the mouse. The separation and purification method for the islet cells of the mouse, provided by the invention, is simple and feasible in operation, and the separated islet cells have no obvious differences with those obtained by a CBD method in forms and functions, so that the selectable technologies for separation of pancreas islet of the mouse are enriched, and the separative power is improved.
Owner:SHANGHAI INST FOR ENDOCRINE & METABOLIC DISEASES
Method for obtaining analogous retinal tissues rich in cone and rod cells by using human induced pluripotent stem cells
ActiveCN108795864AShort digestion timeHigh activityNervous system cellsArtificially induced pluripotent cellsMatrigelOptic tectum
The invention discloses a mmethod for obtaining analogous retinal tissues rich in cone and rod cells by using human induced pluripotent stem cells. The method comprises the following steps: digestinghiPSCs to obtain cell pellets, and then carrying out suspension cultivation on the cell pellets to obtain an embryoid body; reinoculating the embryoid body into a culture dish enveloped by Matrigel, and carrying out induced differentiation inside an induced cultivation solution to obtain nerve retina (NR) and RPE; stirring up the NR and the RPE, carrying out suspension cultivation to obtain 3D analogous retina comprising NR tissues, then carrying out continuous suspension cultivation inside an optimized culture solution without retinoic acid, carrying out NR differentiation on all retinal cells, comprising highly matured photoreceptor cells, Rhodopsin positive rod cells, L / M OPSIN positive red and green cone cells and S-OPSIN positive blue cone cells, and thus particularly obtaining the analogous retinal tissues rich in red and green cone and rod cells.
Owner:ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV +1
Mixed enzyme for skin tissue dissociation, preparation method of mixed enzyme, dissociation kit and dissociation method
ActiveCN112708610AHigh activityImprove experimental efficiencyCell dissociation methodsEpidermal cells/skin cellsDNA - Deoxyribonucleic acidDeoxyribonuclease I
The invention discloses a mixed enzyme for skin tissue dissociation. The mixed enzyme is composed of collagenase II, trypsin and deoxyribonuclease I, wherein the concentration of the collagenase II is 2-4 mg / mL; the concentration of the trypsin is 1-3 mg / mL; and the concentration of the deoxyribonuclease I is 0.01-0.05 mg / mL. Besides, the invention further discloses a dissociation method for the skin tissue. The skin tissue is pretreated with a specific buffer solution, and tissue dissociation is conducted through the specific mixed enzyme, so that a skin tissue single-cell suspension with large number of single cells, good activity, few fragments and low clustering rate can be efficiently, rapidly and stably obtained. The screened and optimized mixed enzyme and concentration have higher pertinence and higher efficiency; more costs and time are saved compared with those in a method of combining a few collagenases in a broad spectrum; and a good tool is provided for single-cell related research of a skin tissue sample which is always difficult to dissociate.
Owner:上海伯豪生物技术有限公司
Method for determining lead content of feed mildew preventive
InactiveCN105628633AImprove stabilityGood precisionColor/spectral properties measurementsPotassium ferricyanideDigestion
The invention discloses a method for determining the lead content of a feed mildew preventive. According to the method, wet digestion is conducted on the feed mildew preventive by using nitric acid, hydrochloric acid and hydrofluoric acid to obtain a digestion solution, hydroboron, potassium ferricyanide, sodium hydroxide and a lead stabilizer are prepared into a lead determination reagent, the digestion solution and the lead determination reagent are carried into a chemical atomizer for hydride generation, hydride is led to an atomic absorption spectrophotometer by using carrier gas to perform flame atomization, then the absorbance value of a lead element at the position of 283.3 nm wavelength is determined, and the lead content of the feed mildew preventive is obtained through calculation based on an established standard curve. The method enables the detection limit of the lead to reach the level ppb and can achieve trace detection, and the relative error of a parallel determination result is lower than 10%, the accuracy of the detection result is good, and the detection data is more real and reliable.
Owner:CHONGQING MINTAI NEW AGROTECH DEV GRP CO LTD
Rapid detection method for fertilizer nutrients
InactiveCN102426128ARich varietyGood compatibilityPreparing sample for investigationPotassiumNitrogen
The present invention discloses a rapid detection method for fertilizer nutrients. According to the method, 1-5 mL of concentrated sulfuric acid is added during the sample digesting process, 0-2 mL of perchloric acid (or hydrogen peroxide) is added as a digestion agent, and the resulting digested solution requiring detection can be used for colorimetric determinations or turbidimetric determinations of nitrogen, phosphorus and potassium. The method of the present invention has advantages of wide application range, stable reagent, low cost, easy availability, short digestion time, complete digestion, convenient operation, high accuracy, time saving, labor saving, and the like. With the method of the present invention, the nitrogen content, the phosphorus content and the potassium content in the fertilizer can be rapidly and efficiently detected, such that the rapid detection requirements of the fertilizer sample during the fertilizer production process and the fertilizer sale process are met, and the purposes of time saving, labor saving, convenience, rapidness, accuracy and reliability are achieved.
Owner:赵玉正
Agent special for killing rodent and method for preparing the same
InactiveCN101112198AA large digestive systemShort digestion timeBiocidePest attractantsSlurryFood flavor
The invention relates to a non-toxic special pesticide for rodent and a preparation method thereof. The non-toxic special pesticide for rodent of the invention is made of such components according to the weight proportions as cement of 60-80, edible fat of 20-40. In addition, anti-corrosion agent, weight proportion regulating agent, seasoning agent, oil antioxidant agent, favoring agent, food flavor and so on can also be added. The preparation method is as follows: 1. the edible fat is cut into particle to be refined to have liquid oil out by ordinary refine method, then the liquid oil is separated from oil residue after the temperature of the liquid oil is increased to 85-95 DEG C; 2. the cement powder is added to the liquid oil and the mixture is stirred sufficiently to get a slurry matter, and the heating is not stopped until the temperature is increased to 105 DEG C; 3. the separated oil residue is added to the slurry matter and then stirred sufficiently after the temperature of the slurry matter decreased to below 95 DEG C. 4. the obtained material is cooled, made into grain and packed.
Owner:王及伟 +1
Method of analyzing rare earth element contents in crude oil by microwave digestion ICP-MS (Inductively coupled plasma mass spectrometry)
InactiveCN110568054AReduce dosageShort digestion timeMaterial analysis by electric/magnetic meansRare-earth elementInternal standard
The invention discloses a method of analyzing rare earth element contents in crude oil by microwave digestion ICP-MS (Inductively coupled plasma mass spectrometry), and belongs to the field of detection of rare earth element contents in crude oil. The method specifically includes the following steps: using a dispersant to dissolve a crude oil sample to enable all components in the crude oil to beuniformly distributed; configuring a sample digestion solution; configuring a standard solution used for testing; configuring an internal standard solution; and detecting the contents of rare earth elements in the crude oil. The method uses the dispersant to dissolve the crude oil sample to enable all the components in the crude oil to be uniformly distributed, then completely digest the sample through a nitric acid-hydrogen peroxide digestion system under high temperature and high pressure, and has the characteristics of small reagent use amounts, short digestion time, low impurity interference, simple and convenient operation, good repeatability and the like.
Owner:CHINA PETROLEUM & CHEM CORP +1
Water quality COD (chemical oxygen demand) on-line analyzer digestor
InactiveCN103257071AAvoid failureHeating evenlyPreparing sample for investigationBiochemical engineeringWater quality
The invention discloses a water quality COD (chemical oxygen demand) on-line analyzer digestor which comprises a first electromagnetic valve, a second electromagnetic valve, a temperature sensor, a digestion tube and a shell, wherein the shell is internally provided with the digestion tube; the digestion tube is provided with the temperature sensor; two ends of the digestion tube are respectively connected with the first electromagnetic valve and the second electromagnetic valve; the first electromagnetic valve is arranged on the lower end outside the shell; the second electromagnetic valve is arranged on the upper end outside the shell; and a micro wave radiator is arranged outside the digestion tube and inside the shell. The water quality COD on-line analyzer digestor provided by the invention is heated up by adopting micro waves, can prevent failure caused by oxidation and corrosion of heating wires, is heated uniformly with high heating efficiency, and has good digestion consistence.
Owner:NANJING NORMAL UNIVERSITY
Method for detecting crude proteins in castor-oil plant seeds or castor bean meal
ActiveCN107356702AShort digestion timeGuaranteed accuracyChemical analysis using titrationPreparing sample for investigationChemistryDigestion
The invention relates to a method for detecting crude proteins in castor-oil plant seeds or castor bean meal. The method comprises the following steps: adding copper sulfate, potassium sulfate and concentrated sulfuric acid into a sample to be detected, fully mixing the sample to be detected and the added substances to make the sample to be detected completely wet by the concentrated sulfuric acid; and adding hydrogen peroxide, heating all above materials, carrying out full digestion, and detecting the content of the crude proteins in the above obtained digestion solution. Compared with the prior art, the method disclosed in the invention has the advantages of small relative error, short digestion time, and increase of the digestion speed on the premise of guaranteeing the accuracy. The method allows the digestion time to be short and the detection speed to be fast during the determination of the crude proteins in high-oil plants, and the use amount of a catalyst in the method is very small, so the method is of great realistic significance ecologically and economically.
Owner:新疆农业科学院生物质能源研究所
Method for in-vitro isolated culture of chicken fallopian tube epithelial cells
InactiveCN105695396AGood effectFast growthCulture processEpidermal cells/skin cellsParenchymaDigestion
The invention provides a method for in-vitro isolated culture of chicken primary fallopian tube epithelial cells.The method includes the following steps that 1, tissue is aseptically separated from the neck of a fallopian funnel, close to a follicle, of a laying hen; 2, the tissue obtained after separation is digested by pancreatic enzymes and EDTA jointly, and the chicken fallopian tube epithelial cells are obtained; 3, at the temperature of 37 DEG C, the chicken fallopian tube epithelial cells are incubated for 2 h; 4, the cells obtained after incubation in the step 3 are cultured.The primary chicken fallopian tube epithelial cells obtained after isolated culture in the method are good in effect, high in growth speed and good in state; digestion time is short, the result is good, fewer parenchyma cells exist, contamination to fibroblasts is avoided, and healthy epithelioid cell populations can be obtained.
Owner:HENAN AGRICULTURAL UNIVERSITY
Ultraviolet digestion method for EDTMPA (ethylenediamine tetramethylenephosphonic acid)
InactiveCN108190999ANo significant effect on the digestion rateSimple methodWater/sewage treatment by irradiationSpecific water treatment objectivesLow voltageWastewater
The invention relates to an ultraviolet oxidation based digestion method for EDTMPA (ethylenediamine tetramethylenephosphonic acid) in wastewater. According to the method, sodium persulfate is added to the wastewater containing EDTMPA, the wastewater is irradiated by a 10W low-voltage mercury lamp, and digestion rate of organophosphorus EDTMPA reaches 96% or higher. The method is simple to operate, low in energy consumption, energy-saving and environmentally friendly, digestion is fast, and pH regulation is not required.
Owner:SHANDONG UNIV +1
Human primary gastric cancer cell separation and culture method
InactiveCN108070560AHigh purityA large amountCell dissociation methodsCell culture supports/coatingTissue ProcessorDigestion
The invention provides a human primary gastric cancer cell separation and culture method. The method comprises the following steps of (1) disinfecting a tissue processing device; (2) using a pair of operating scissors for separating a gastric cancer tissue, placing the tissue into a precooled sterile PBS liquid containing double antibodies, and washing for multiple times to remove bloodstains andnecrotic tissues; (3) cutting up the tissues, and using preheated IV type collagenase and trypsin for digesting respectively; (4) terminating digestion, centrifuging, and removing a supernatant; (5) after carrying out cell counting, inoculating into a coated culture flask; (6) culturing at 37 DEG C in an environment with 5 percent of CO2; (7) purifying the cells; (8) subculturing the tissues; (9)observing cell morphology; (10) determining cell survival rate; (11) carrying out immunological identification. The invention provides the human primary gastric cancer cell separation and culture method which is simple to operate, the number of the obtained cells is large, the survival rate is high, the method is the ideal human gastric cancer cell primary separation and culture method, and a reliable cell resource is provided for experiments.
Owner:JIANGYIN CHI SCI
Human adipose derived stromal cell separation and culture method
InactiveCN109797136ALow toxicityShort digestion timeSkeletal/connective tissue cellsLYMPHOCYTE SEPARATION MEDIUMDigestion
The invention discloses a human adipose derived stromal cell separation and culture method which includes the steps: firstly, fat collection; secondly, removing impurities in fat; thirdly, digestion by mixed enzyme; fourthly, collecting and centrifuging; fifthly, culture and passage; sixthly, digestion and freezing. According to the human adipose derived stromal cell separation and culture method,separation is implemented by the mixed enzyme, digestion time is short, acquired cells cannot be further purified by a lymphocyte separation medium and a filter screen, and liquid needs to be changedafter the cells fits with wall. According to the method, a separation process is low in cost, used reagents and materials are less in separation, operation processes are simplified, pollution risks in the operation process are reduced, animal source serums are omitted in the whole separation and culture process, cell toxicity is reduced, and a culture period is short by the aid of the mixed enzyme and a serum-free culture system.
Owner:湖南南华生物技术有限公司
Agent special for killing rodent and method for preparing the same
InactiveCN100490651CA large digestive systemShort digestion timeBiocideAnimal repellantsPreservativeSlurry
Owner:王及伟 +1
Renal tubular epithelial cell separation and culture method
PendingCN114045255AIncrease cell viabilityProliferate fastCell dissociation methodsArtificial cell constructsRenal Tubular Epithelial CellsNutrition
The invention discloses a renal tubular epithelial cell separation and culture method which comprises the following steps: S1, mechanical tissue separation: taking a renal tissue sample, separating a cortex part, and then cutting into pieces; s2, enzymolysis: digesting with a mixed enzyme, and centrifuging; and S3, cell culture: resuspending the cell precipitate by using a complete culture medium, and inoculating the cell precipitate into a culture bottle. Wherein mechanical separation is simple, and mechanical damage is reduced to the maximum extent. The best digestion efficiency is achieved through mild enzymolysis, the obtained cells are high in activity, the activity of the obtained cells can reach 50% or above, the number of the cells at least reaches 1 * 10 < 6 > / g, the cells have high proliferation performance and high initial inoculation density, the cell proliferation environment pressure is small, a simple and convenient nutrient medium rich in nutrition is added, substrate layer planking is not needed, and the cost is low. The cell proliferation speed is at least one time faster than that reported in the literature; and if 1 * 10<6> cells are inoculated in a T75 bottle, complete convergence can be achieved within 72 hours.
Owner:广州华越肾科再生医学科技有限公司
Method for determining COD through rapid digestion
InactiveCN106092719APrevent splashResidue reductionChemical analysis using titrationPreparing sample for investigationPhosphoric acidDigestion
The invention relates to a method for determining COD through rapid digestion. The method comprises the steps of carrying out rapid digestion on a water sample in a phosphoric acid medium under the condition of refluxing in a manner of taking a potassium dichromate solution as an oxidant and taking a MgSO4-MnSO4 mixture as a catalyst, cooling the water sample to room temperature, then, carrying out titration by using a sodium thiosulfate solution till an end by using ferroin as an indicator, and figuring out a COD value according to an equation. According to the method, sulfuric acid-silver sulfate is not used as a cocatalyst, and mercuric sulfate is not used as a chloride ion masking agent, so that the residual of heavy metals in the digested sample is greatly lowered, and aftertreatment on detected water samples and environmental protection are facilitated; meanwhile, the digestion time is short, the accuracy of detection is high, the operation is facilitated, and the labor intensity is lowered, so that the method can serve as a routine method for detecting the COD.
Owner:贵州开磷质量检测中心有限责任公司
Forensic medical diatom inspection method based on mixed acid digestion
ActiveCN102507585BAvoid damageReduce lossesPreparing sample for investigationMaterial analysis by optical meansEpoxyFiltration
Owner:GUANGZHOU CRIMINAL SCI & TECH RES INST
Technology and process for treating organic solid waste through self-balancing fermentation and incineration
PendingCN112159823AImprove utilization efficiencyAchieve self-balancingWaste based fuelFermentationProcess engineeringEnvironmental engineering
The invention relates to a technology and a process for treating organic solid waste through self-balancing fermentation and incineration. According to the technology, organic solid waste is adopted to cooperate with anaerobic fermentation, biogas generated after fermentation is purified and then used by a subsequent incineration system, and remaining yeast residues (containing yeast liquid) are mixed with a certain amount of auxiliary materials and then incinerated. Due to the fact that the materials are fully fermented before incineration, macromolecular combustible components in the materials are converted into micromolecular fuel, the utilization efficiency of the energy conversion process is improved, part of the generated biogas can cover fuel consumption needed by the system, part of the generated biogas is used for power consumption of a power generation coverable system, and therefore self-balancing of system energy is achieved.
Owner:CHONGQING UNIV
Compound enzyme digestive juice as well as preparation method and application thereof
ActiveCN112481190AShort digestion timeEasy to operateCell dissociation methodsVertebrate cellsSucroseRibonuclease
The invention provides a compound enzyme digestive juice as well as a preparation method and an application thereof. The digestive juice comprises the following components with final concentrations: 100-200U / mL of type IV collagenase, 1-10U / ml of type II dispersive enzyme, 50-200U / ml of deoxyribonuclease I, 0.1-0.5 mM of PMSF, 10wt%-20wt% of ethylene glycol, 5wt%-10wt% of polysucrose, and sterilewater serving as a solvent, wherein the pH value of the digestive juice is 7.4-8.0. The preparation method comprises the following steps: preparing materials according to the final concentration of each component of the digestive juice; dissolving type IV collagenase, type II dispersive enzyme, deoxyribonuclease I, PMSF and polysucrose in sterile water, adding ethylene glycol, and uniformly mixing; and adjusting the pH value to 7.4-8.0, filtering and sterilizing to obtain the product. The compound enzyme digestive juice and the preparation method thereof are applied to the organoids technology. The digestive juice disclosed by the invention not only can be used for obtaining complete organoid, but also can be used for obtaining cell clusters with uniform dispersion sizes, and is short in digestion time, convenient to operate and stable in effect.
Owner:ACCURATE INT BIOTECHNOLOGY (GUANGZHOU) CO LTD
Method for rapidly determining total nitrogen content in water by using resorcinol
PendingCN114235732AImprove stabilityImprove test efficiencyMaterial analysis by observing effect on chemical indicatorPreparing sample for investigationDigestionSulfuric acid
The invention relates to a method for rapidly determining the content of total nitrogen in water by using resorcinol. The method comprises the following steps: step 1, preparing a digestion solution; weighing 10 g of sodium hydroxide solid and 40 g of potassium persulfate solid, dissolving with deionized water, and diluting to 1000 ml; 2, digesting the sample; putting 1ml of the sample into a colorimetric tube; adding 1ml of digestion solution into the colorimetric tube, controlling the digestion temperature at 105-110 DEG C, and digesting for 10 minutes; then taking out the colorimetric tube, adding 1ml of digestion solution into the colorimetric tube, and digesting at 105-110 DEG C for 5-20 minutes; 3, testing the absorbance of the sample; transferring 1.0 ml of the digested sample into a 10ml color distinguishing tube, adding 1ml of a resorcinol solution with the mass fraction of 0.5% and 3ml of concentrated sulfuric acid, and fully mixing the solution; after the sample is cooled to the room temperature, diluting to a 10ml scale line, and measuring the absorbance; and 4, comparing the absorbance of the sample with the standard curve, and checking the total nitrogen content of the sample. The digestion solution is prepared and mixed in advance, so that the digestion reaction rate is increased, the reaction is sufficient, the stability of experimental data is good, and the applicability is high.
Owner:山东中节能天融环保技术有限公司
Method for extracting subtotipotent stem cells from placental fetal face chorion
ActiveCN105200007BReduce processingReduce manpowerNervous disorderGenetic material ingredientsFiltrationMagnetic bead
Owner:BOYALIFE
Detection method for measuring tin content in soil by an inductively coupled plasma mass spectrometry
InactiveCN110308197AProlong digestion timeShort digestion timePreparing sample for investigationMaterial analysis by electric/magnetic meansInductively coupled plasma mass spectrometryTandem mass spectrometry
The invention provides a detection method for measuring tin content in soil by an inductively coupled plasma mass spectrometry. The method comprises the following steps: preparation of a standard usesolution, preparation of a standard solution, preparation of a test solution, preparation of a reagent blank solution, determination and the like.
Owner:福建省农业科学院农业质量标准与检测技术研究所
Large-scale preparation method of placenta mesenchymal stem cells
InactiveCN101921728AEasy to drawCutting saves time and effortEmbryonic cellsGerm cellsMesenchymal stem cellPollution
The invention relates to a preparation method of placenta mesenchymal stem cells. The method routinizes the preparation process, simplifies operation steps, shortens the operation time and reduces the workload, thereby preventing pollution to a great degree, reducing reagent and material required in the preparation process and increasing the yield of mesenchymal stem cells.
Owner:青岛奥克生物开发有限公司
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Patsnap Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com