50results about How to "Short digestion time" patented technology

The invention discloses an umbilical cord tissue mesenchymal stem cell isolated culture method. The method comprises the steps of A, sample collection and cell isolated culture; B, subculture, wherein after being cultured, primary cells are subcultured continuously when cell confluence reaches over 80%; C, growth curve drawing; D, cell detection, wherein the third generation of cells are adopted for detection, well digested third generation of umbilical cord mesenchymal stem cells are prepared and washed with a buffer solution, then antibody labeling is conducted on cells, incubation washing is conducted, and then detection is conducted. Two types of collagenase are used at the same time, and digestion of umbilical cord tissue is conducted with hyaluronidase and neutral protease or accutase, so that tissue blocks are quickly and fully digested.

The invention discloses a forensic medical diatom inspection method based on mixed acid digestion. The forensic medical diatom inspection method comprises the steps of optical microscope inspection and scanning electron microscope inspection. The optical microscope inspection at least comprises the steps of: (1) performing a blank test; (2) performing mixed acid digestion by adding concentrated nitric acid, concentrated hydrochloric acid and a hydrogen peroxide solution in a sample to be tested and digesting in a manner of heating in water bath; (3) centrifuging and precipitating; (4) coating on a sheet and sealing by using epoxy resin; and (5) performing diatom qualitative and quantitative analysis. The scanning electron microscope inspection at least comprises the steps of: (1) performing a blank test; (2) performing mixed acid digestion by adding concentrated nitric acid, concentrated hydrochloric acid and a hydrogen peroxide solution in a sample to be tested and digesting in a manner of heating in water bath; (3) performing vacuum filtration; (4) automatically taking pictures and storing images by a scanning electron microscope; and (5) performing diatom qualitative and quantitative analysis. The forensic medical diatom inspection method based on the mixed acid digestion, disclosed by the invention, is high in sensitivity, efficient, safe, environment-friendly and exact in qualitative and quantitative analysis, and has good application prospect in the forensic medical drowning diagnosis practice.

The invention provides a method for measuring the total content of nitrogen of fresh water quality. The method comprises the steps of sampling, preparing an alkaline potassium persulfate solution, preparing a potassium nitrate standard solution, preparing a hydrochloric acid solution, drawing a calibration curve and detecting a water sample to be measured. According to the method, a polytetrafluoroethylene decomposition bottle is adopted to replace a colorimetric tube, so that the decomposition process is relatively sealed and relatively fully carried out, and the decomposition time is relatively short; cross contamination is not caused between the inside and the outside of the decomposition bottle, and interference is eliminated, so that the result is relatively accurate, and the operation is relatively simple and relatively convenient; furthermore, an opening or a plug of the colorimetric tube is prevented from being broken in the decomposition process, so that the problem of analyzing by sampling again is avoided. Furthermore, after the colorimetric tube is replaced by the decomposition bottle, and a pipette is also used, the sample can be analyzed at the constant volume without being transferred into the colorimetric tube after being decomposed, so that the method is convenient, fast and easy to operate, and the accuracy is improved.

The invention relates to a method for extracting sub-totipotent stem cells from a chorion of a fetal surface of a placenta. The method comprises the steps of removing an amnion and stagnated blood from the placenta, and then repetitively washing the surface of the placenta to perform sterilization; shearing the chorion of the fetal surface in a glass utensil, removing placenta lobule tissues remained on the surface as much as possible, and shearing the chorion into small blocks; washing small tissue blocks by using a screen and a great amount of normal saline, and removing residual blood cells; performing tissue digestion: performing oscillatory digestion in a constant temperature shaker by using mixed enzymes; adding a proper amount of FBS for termination after digestion, performing filtration through a filter screen, and adding a great amount of normal saline to wash filter residues to obtain cells as many as possible; performing centrifugation, abandoning supernatant, adding saline water for washing and performing centrifugation to obtain mononuclear cells; performing magnetic bead sorting (OCT-4 positive, Nanog positive and STRO-1 negative) to obtain target cells. The invention further relates to the sub-totipotent stem cells obtained by adopting the method and pharmaceutical use thereof. The sub-totipotent stem cells have excellent characteristics.

The invention relates to a method for digesting a sample in the process of measuring chemical oxygen demand (COD). The method comprises the following steps of: taking 10.00ml of COD standard sample, and taking distilled water in blank; adding 5.00ml of 0.0250mol/L potassium dichromate standard solution into a water sample; respectively adding 3.3ml of 50g/L manganese sulfate solution, 1.0ml of 50g/L cerium sulfate solution and 18ml of concentrated sulfuric acid, and mixing; putting into a 160 DEG C drying box, and heating for 30 minutes; cooling to room temperature, adding 3 drops of 1,10-phenanthroline-ferrous indicator, titrating by using 0.02510mol/L ammonium ferrous sulfate, and taking a point at which the color of solution is turned from yellow to blue green to red brown as a finishing point; and recording the consumption milliliter number V1 of ammonium ferrous sulfate standard titration solution and a blank test titration consumption milliliter number V0. By the method for digesting the sample, the common drying box is used for heating, the operation process is simple and is easy to master, acid consumption is lower than that in a classical method, digestion time is short, and batch processing can be realized.

The invention belongs to the field of sludge treatment and relates to a municipal sludge energy treatment system. The system comprises an acidogenic reactor and a methanogenic reactor; and first-level membrane separation equipment and second-level membrane separation equipment are arranged between the acidogenic reactor and a methanogenic reactor. The sludge passes through the acidogenic reactor and is hydrolyzed to generate acid in the methanogenic reactor, and then passes through the first-level membrane separation equipment to be separated into first concentrated solution and first penetrating fluid. The first concentrated solution reflows to the acidogenic reactor, and the first penetrating fluid enters the second-level membrane separation equipment to be separated into second concentrated solution and second penetrating fluid; and the second concentrated solution enters the methanogenic reactor to be fermented to generate methane. By organically combining the two-phase anaerobic digestion technology with the membrane separation technology, the municipal sludge energy treatment system realizes the combination of a physical dialysis method and a kinetic control method and really realizes the separation of fermentative bacteria bacteria and methanogenic bacteria. Therefore, the bacteria in two phases can exert the maximum activity, and the operating stability and energy efficiency of the whole system are improved.

The invention belongs to the technical field of cell separation, and particularly relates to a separation method for islet cells of a mouse. The method comprises the following steps: narcotizing the mouse, disinfecting the skin and cutting along the center of the abdomen to fully expose the pancreas; shearing the ligament organization connected with the pancreas of the spleen, clamping to close the junction of the splenic vein and the spleen and cutting the heart broken, inserting a needle at the intersection of the splenic vein and the portal vein to the splenic vein, and injecting collagenase filling liquid; immersing the filled pancreas of the spleen into the collagenase filling liquid after the pancreas of the spleen is dissociated from the abdominal cavity; performing water bath slaking on the pancreas collagenase solution, performing centrifugal separation after adding Hank's liquid and taking the sediment; and performing heavy-suspending sedimentation, filtration and centrifugal treatment on the sediment to obtain the islet cells of the mouse. The separation and purification method for the islet cells of the mouse, provided by the invention, is simple and feasible in operation, and the separated islet cells have no obvious differences with those obtained by a CBD method in forms and functions, so that the selectable technologies for separation of pancreas islet of the mouse are enriched, and the separative power is improved.

The invention relates to a non-toxic special pesticide for rodent and a preparation method thereof. The non-toxic special pesticide for rodent of the invention is made of such components according to the weight proportions as cement of 60-80, edible fat of 20-40. In addition, anti-corrosion agent, weight proportion regulating agent, seasoning agent, oil antioxidant agent, favoring agent, food flavor and so on can also be added. The preparation method is as follows: 1. the edible fat is cut into particle to be refined to have liquid oil out by ordinary refine method, then the liquid oil is separated from oil residue after the temperature of the liquid oil is increased to 85-95 DEG C; 2. the cement powder is added to the liquid oil and the mixture is stirred sufficiently to get a slurry matter, and the heating is not stopped until the temperature is increased to 105 DEG C; 3. the separated oil residue is added to the slurry matter and then stirred sufficiently after the temperature of the slurry matter decreased to below 95 DEG C. 4. the obtained material is cooled, made into grain and packed.

The invention discloses a method of analyzing rare earth element contents in crude oil by microwave digestion ICP-MS (Inductively coupled plasma mass spectrometry), and belongs to the field of detection of rare earth element contents in crude oil. The method specifically includes the following steps: using a dispersant to dissolve a crude oil sample to enable all components in the crude oil to beuniformly distributed; configuring a sample digestion solution; configuring a standard solution used for testing; configuring an internal standard solution; and detecting the contents of rare earth elements in the crude oil. The method uses the dispersant to dissolve the crude oil sample to enable all the components in the crude oil to be uniformly distributed, then completely digest the sample through a nitric acid-hydrogen peroxide digestion system under high temperature and high pressure, and has the characteristics of small reagent use amounts, short digestion time, low impurity interference, simple and convenient operation, good repeatability and the like.

The invention discloses a water quality COD (chemical oxygen demand) on-line analyzer digestor which comprises a first electromagnetic valve, a second electromagnetic valve, a temperature sensor, a digestion tube and a shell, wherein the shell is internally provided with the digestion tube; the digestion tube is provided with the temperature sensor; two ends of the digestion tube are respectively connected with the first electromagnetic valve and the second electromagnetic valve; the first electromagnetic valve is arranged on the lower end outside the shell; the second electromagnetic valve is arranged on the upper end outside the shell; and a micro wave radiator is arranged outside the digestion tube and inside the shell. The water quality COD on-line analyzer digestor provided by the invention is heated up by adopting micro waves, can prevent failure caused by oxidation and corrosion of heating wires, is heated uniformly with high heating efficiency, and has good digestion consistence.

The invention relates to a method for detecting crude proteins in castor-oil plant seeds or castor bean meal. The method comprises the following steps: adding copper sulfate, potassium sulfate and concentrated sulfuric acid into a sample to be detected, fully mixing the sample to be detected and the added substances to make the sample to be detected completely wet by the concentrated sulfuric acid; and adding hydrogen peroxide, heating all above materials, carrying out full digestion, and detecting the content of the crude proteins in the above obtained digestion solution. Compared with the prior art, the method disclosed in the invention has the advantages of small relative error, short digestion time, and increase of the digestion speed on the premise of guaranteeing the accuracy. The method allows the digestion time to be short and the detection speed to be fast during the determination of the crude proteins in high-oil plants, and the use amount of a catalyst in the method is very small, so the method is of great realistic significance ecologically and economically.

The invention relates to an ultraviolet oxidation based digestion method for EDTMPA (ethylenediamine tetramethylenephosphonic acid) in wastewater. According to the method, sodium persulfate is added to the wastewater containing EDTMPA, the wastewater is irradiated by a 10W low-voltage mercury lamp, and digestion rate of organophosphorus EDTMPA reaches 96% or higher. The method is simple to operate, low in energy consumption, energy-saving and environmentally friendly, digestion is fast, and pH regulation is not required.

The invention discloses a human adipose derived stromal cell separation and culture method which includes the steps: firstly, fat collection; secondly, removing impurities in fat; thirdly, digestion by mixed enzyme; fourthly, collecting and centrifuging; fifthly, culture and passage; sixthly, digestion and freezing. According to the human adipose derived stromal cell separation and culture method,separation is implemented by the mixed enzyme, digestion time is short, acquired cells cannot be further purified by a lymphocyte separation medium and a filter screen, and liquid needs to be changedafter the cells fits with wall. According to the method, a separation process is low in cost, used reagents and materials are less in separation, operation processes are simplified, pollution risks in the operation process are reduced, animal source serums are omitted in the whole separation and culture process, cell toxicity is reduced, and a culture period is short by the aid of the mixed enzyme and a serum-free culture system.

The invention discloses a renal tubular epithelial cell separation and culture method which comprises the following steps: S1, mechanical tissue separation: taking a renal tissue sample, separating a cortex part, and then cutting into pieces; s2, enzymolysis: digesting with a mixed enzyme, and centrifuging; and S3, cell culture: resuspending the cell precipitate by using a complete culture medium, and inoculating the cell precipitate into a culture bottle. Wherein mechanical separation is simple, and mechanical damage is reduced to the maximum extent. The best digestion efficiency is achieved through mild enzymolysis, the obtained cells are high in activity, the activity of the obtained cells can reach 50% or above, the number of the cells at least reaches 1 * 10 < 6 >/g, the cells have high proliferation performance and high initial inoculation density, the cell proliferation environment pressure is small, a simple and convenient nutrient medium rich in nutrition is added, substrate layer planking is not needed, and the cost is low. The cell proliferation speed is at least one time faster than that reported in the literature; and if 1 * 10<6> cells are inoculated in a T75 bottle, complete convergence can be achieved within 72 hours.

The invention relates to a method for determining COD through rapid digestion. The method comprises the steps of carrying out rapid digestion on a water sample in a phosphoric acid medium under the condition of refluxing in a manner of taking a potassium dichromate solution as an oxidant and taking a MgSO4-MnSO4 mixture as a catalyst, cooling the water sample to room temperature, then, carrying out titration by using a sodium thiosulfate solution till an end by using ferroin as an indicator, and figuring out a COD value according to an equation. According to the method, sulfuric acid-silver sulfate is not used as a cocatalyst, and mercuric sulfate is not used as a chloride ion masking agent, so that the residual of heavy metals in the digested sample is greatly lowered, and aftertreatment on detected water samples and environmental protection are facilitated; meanwhile, the digestion time is short, the accuracy of detection is high, the operation is facilitated, and the labor intensity is lowered, so that the method can serve as a routine method for detecting the COD.

The invention relates to a method for rapidly determining the content of total nitrogen in water by using resorcinol. The method comprises the following steps: step 1, preparing a digestion solution; weighing 10 g of sodium hydroxide solid and 40 g of potassium persulfate solid, dissolving with deionized water, and diluting to 1000 ml; 2, digesting the sample; putting 1ml of the sample into a colorimetric tube; adding 1ml of digestion solution into the colorimetric tube, controlling the digestion temperature at 105-110 DEG C, and digesting for 10 minutes; then taking out the colorimetric tube, adding 1ml of digestion solution into the colorimetric tube, and digesting at 105-110 DEG C for 5-20 minutes; 3, testing the absorbance of the sample; transferring 1.0 ml of the digested sample into a 10ml color distinguishing tube, adding 1ml of a resorcinol solution with the mass fraction of 0.5% and 3ml of concentrated sulfuric acid, and fully mixing the solution; after the sample is cooled to the room temperature, diluting to a 10ml scale line, and measuring the absorbance; and 4, comparing the absorbance of the sample with the standard curve, and checking the total nitrogen content of the sample. The digestion solution is prepared and mixed in advance, so that the digestion reaction rate is increased, the reaction is sufficient, the stability of experimental data is good, and the applicability is high.

The invention provides a detection method for measuring tin content in soil by an inductively coupled plasma mass spectrometry. The method comprises the following steps: preparation of a standard usesolution, preparation of a standard solution, preparation of a test solution, preparation of a reagent blank solution, determination and the like.