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47results about How to "Prolong digestion time" patented technology

Diatom inspection method in medical jurisprudence

ActiveCN101776623ADoes not affect detectabilityDoes not affect species identificatioMaterial analysis using wave/particle radiationPreparing sample for investigationFiltrationScanning electron microscope
The invention relates to the field of medical jurisprudence inspection, in particular to a diatom inspection method in medical jurisprudence. The method at least comprises the following steps: (1) microwave digestion: adding concentrated nitric acid and hydrogen peroxide solution into the inspected sample to carry out microwave digestion; (2) vacuum filtration: carrying out vacuum filtration on the digestion solution, adding ultrapure water, carrying out vacuum filtration continually until the surface of the filter membrane is approximately neutral, adding absolute ethyl alcohol, and carrying out vacuum filtration to remove moisture from the filter membrane; (3) a scanning electron microscope automatically takes pictures and stores the pictures; and (4) qualitative and quantitative analysis on diatom: carrying out inspection, classification and statistic treatment on the diatom in the on-spot pictures in an artificial identification way or computer automated identification way. The invention has the advantages of high detection sensitivity, high accuracy of qualitative and quantitative analysis, simpleness, high efficiency and environmental protection, can effectively avoid pollution, greatly improves the working environment for diatom inspection technicians in medical jurisprudence, and reduces the labor intensity, thereby having wide application prospects in drowning diagnosis practice in medical jurisprudence.
Owner:GUANGZHOU CRIMINAL SCI & TECH RES INST

Extraction method of apostichopus japonicus body-wall total RNA

InactiveCN101864414AMeet Gene Expression AnalysisFulfil requirementsDNA preparationWater bathsTotal rna
The invention discloses a high-extraction-purity, good-integrity and high-yield extraction method of apostichopus japonicus body-wall total RNA (Ribonucleic Acid). The method comprises the following steps of: quickly freezing apostichopus japonicus body-wall tissue in liquid nitrogen; grinding and putting the frozen tissue in lysate to homogenate, centrifugate and take supernatant fluid; adding chloroform to the supernatant fluid and centrifugating to take the supernatant fluid to another centrifuge tube; then, adding a high-salt solution and isopropyl alcohol and filtering precipitation with ethanol; dissolving the precipitation with DEPC (diethypyrocarbonate) treated water to have constant volume; sequentially adding a DNA enzyme buffer solution without RNA enzymes, DNA enzymes without RNA enzymes and an RNA enzyme inhibitor to the dissolved solution to mix; carrying out a water bath at 37 DEG C to obtain DNA lysate; adding phenol and chloroform in a ratio of 5:1 to the DNA lysate to mix, centrifugate and take supernatant fluid; adding a glycogen solution, a potassium acetate solution and pre-cooling ethanol to the supernatant fluid; mixing and staying over night; centrifugating to discard the supernatant fluid; washing and dying the precipitation with ethanol; dissolving the solution with the DEPC treated water to have constant volume of 20 mu l; and storing at 80 DEG C below zero.
Owner:DALIAN OCEAN UNIV

Preparation method for producing autoclaved aerated concrete block by using antimony ore tailing

InactiveCN104478329AProlong digestion timeReduce condensation thickeningSolid waste managementCeramicwareDry weightSlurry
The invention discloses a preparation method for producing an autoclaved aerated concrete block by using antimony ore tailings. The preparation method comprises the following steps: (1) drying and grinding the antimony ore tailings, and then screening to obtain standby antimony ore tailings with particle diameters less than or equal to 0.150 mm; (2) mixing and stirring the antimony ore tailings obtained from the step (1) with quicklime powder, fly ash, cement, gypsum and water, wherein raw materials are as follows in percentage by weight: 65%-75% of the antimony ore tailings, 13%-23% of the fly ash, 5% of the quicklime powder, 2% of the gypsum and 5% of the cement, and the water-material ratio is 0.60-0.68; then adding a foaming aluminum powder suspension, wherein the weight of aluminum powder contained in the foaming aluminum powder suspension accounts for 0.10%-0.15% of the dry weight of the raw materials; (3) foaming a mixed slurry obtained from the step (2) into a blank, and curing; (4) carrying out autoclaved curing on the blank obtained from the step (3) to obtain the autoclaved aerated concrete block. The preparation method disclosed by the invention can be used for saving the energy resources and the resources by substituting the antimony ore tailings for sand, achieves the harmless and recycling utilization of the antimony ore tailings, accords with the national circular economy industrial policy and achieves outstanding economic benefits, social benefits and environmental benefits.
Owner:WUHAN UNIV OF TECH

Collecting and conveying pipeline segmented pipe cleaning system and method

The invention discloses a collecting and conveying pipeline segmented pipe cleaning system and method. The collecting and conveying pipeline segmented pipe cleaning system comprises at least one intermediate pipe cleaning system arranged along a collecting and conveying trunk line, and the intermediate pipe cleaning system comprises a trunk pipe cleaner staying / transmitting system, a large bypasssystem, a pipe cleaner indicating system and a logic control system. Compared with the prior art, the segmented pipe cleaning system has the positive effects that on the basis of accumulated liquid and slug injection load process analysis, pipeline section plug flow digestion is carried out in a sectional pipe cleaning mode; and by means of inverse-sequence pipe cleaning, the volume of the pipeline after the downstream advance pipe cleaning is utilized to the largest extent, so that the upstream incoming liquid receiving is achieved, the plug flow load of the end point section is reduced, andextension of a large-scale section plug flow catcher is avoided. The pipe cleaning system is simple in structure; and compared with addition of a conventional cleaning station, according to the the collecting and conveying pipeline segmented pipe cleaning system, a pipe cleaner ball collecting barrel, a ball serving barrel and accessory facilities can be avoided, and the system has advantages in large-caliber and high-pressure pipeline engineering projects.
Owner:CHINA PETROLEUM ENG & CONSTR +1

Integrated dry-wet anaerobic digestion device

The invention discloses an integrated dry-wet anaerobic digestion device. The integrated dry-wet anaerobic digestion device comprises a digestion tank, and a feed pipe and a bottom sullage discharge pipe are arranged at the bottom of the digestion tank. The integrated dry-wet anaerobic digestion device is characterized in that a dry anaerobic region, a three-phase separation region and a wet anaerobic region are arranged in the digestion tank from bottom to top; a stirrer is arranged in the dry anaerobic region and carries out material stirring, large-size organic materials are decomposed into small-size materials in the dry anaerobic region, and a great number of biogas bubbles are generated; the large-size solid materials return to the dry anaerobic region through the three-phase separation region, and the small-size materials and liquid phase enter the wet anaerobic region; a biogas aerator pipe is arranged in the wet anaerobic region, an upper discharge pipe is arranged on the upper portion of the wet anaerobic region, water is output through the upper discharge pipe in an overflow mode, the biogas generated under the anaerobic condition enters a top air chamber in the upper portion of the wet anaerobic region, and a top biogas pipe is arranged at the top of the top air chamber. The integrated dry-wet anaerobic digestion device is high in organic matter degradation rate, high in biogas content, low in biogas residue yield, high in outlet water quality, capable of automatically and continuously producing biogas with high-concentration organic solid waste and particularly suitable for anaerobic digestion production of the organic materials which are low in water content and high in impurity content.
Owner:SHANGHAI MUNICIPAL ENG DESIGN INST GRP

Amnion cell separation and collection purification set

An amnion cell separation and collection purification set relates to an amnion cell separation set. The amnion cell separation and collection purification set is used for solving the problems that during amnion cell separation, an amnion is often accumulated in a digestion container, so that a lot of cells are remained among amnion tissues, the amnion cells cannot be efficiently collected, and the obtained cell line is unpurified. The set comprises an amnion cell fixing rotary frame, an amnion tissue digester, an amnion cell separator, a disposable screwed filter, a disposable screwed injector and a disposable screwed centrifuge tube; a threaded circular rod is arranged on the top of a hollow object placing groove of the amnion cell fixing rotary frame; the threaded circular rod is externally connected with a T-shaped handle; a thin plate is arranged in the object placing groove; the amnion tissue digester is of a hollow cube structure; a top cover is arranged on the top of the amnion tissue digester; a liquid inlet screwed opening is formed in one side of the upper part of the amnion tissue digester; a liquid discharge screwed opening is formed in the bottom of the amnion tissue digester; the amnion cell separator is of a hollow cylinder structure; a top cover is arranged on the top of the amnion cell separator; a liquid inlet screwed opening is formed in the side face of the upper part of the amnion cell separator; a liquid discharging screwed opening is formed in the bottom of the amnion cell separator. The amnion cell separation and collection purification set is used for separating and collecting amniotic epithelial cells.
Owner:天晴干细胞股份有限公司

Comprehensive utilization method for potato residues, potato juice and modified starch based on juicing of potatoes

PendingCN107259444APromote staple foodPromote the level of industrializationFood ingredientsSolubilityDigestion
The invention discloses a comprehensive utilization method for potato residues, potato juice and modified starch based on juicing of potatoes. The method comprises the following steps: with cleaned, sorted and sieved potatoes as a raw material, carrying out juicing on the potatoes in the process of extraction of starch from the potatoes; separating starch so as to obtain potato residues and potato juice; and separately conveying the potato residues and the potato juice to respective treating zones for treatment via pipelines or conveyer belts. According to the invention, juicing is carried out on the basis of extraction of starch from the potatoes and then comprehensive treatment is carried out on the products, i.e., the potato residues, the potato juice and the starch, so the industrialization level of the potato industry is improved and application of potatoes as staple food grain is promoted. In the process of processing, flour and potato modified starch adsorb and bond with water molecules, and cross-linking and coagulation occur, so a novel crystal structure is formed; the novel crystal structure is low in solubility in water, enzymatic hydrolysis is reduced, and digestion and absorption are slowed down; and thus, the composition of starch in wheaten food can be changed, and rise in blood sugar and the reaction of plasma insulin after meal are delayed.
Owner:张保华

Separation and purification method of eimeria tenella sporozoite and inoculation method

The invention provides a separation and purification method of eimeria tenella sporozoite and an inoculation method, and relates to the technical field of biology. The separation and purification method comprises the following steps: taking infected chicken manure, adding water, and filtering; centrifuging, adding a saturated saline solution into the precipitate, centrifuging, and adding water into the supernate to obtain an oocyst diluent; centrifuging, and adding chloramine T solution into precipitate to obtain sporulated oocyst suspension; centrifuging, adding saturated saline solution into the precipitate for resuspending, centrifuging and collecting oocyst precipitate; adding a sodium hypochlorite solution for resuspension, and collecting oocysts; and shaking and crushing, stopping shaking when all sporangia escapes, centrifuging to obtain sporangia, adding pancreatin digestive juice for digestion, filtering, centrifuging, and collecting precipitate to obtain eimeria tenella sporozoites. According to the inoculation method, the sporangiums or sporozoites are accurately injected into the middle sections of muscular stomachs and small intestines of the chickens, and successful infection is achieved. The purification degree of the sporangiums and sporozoites obtained by the purification method is high, the experimental operation is simple and convenient, and the infection success rate after injection inoculation reaches 100%.
Owner:SOUTH CHINA AGRI UNIV

A kind of forensic medicine diatom test method

The invention relates to the field of forensic medical inspection, and relates to a forensic medical diatom inspection method. It at least includes the following steps: (1) Microwave digestion: add concentrated nitric acid and hydrogen peroxide solution to the test sample and then carry out microwave digestion; (2) Vacuum suction filtration: carry out vacuum suction filtration on the digestion solution, and then Add ultra-pure water to continue the suction filtration to make the surface of the filter membrane close to neutral, and finally add absolute ethanol to remove the water in the filter membrane by suction filtration; (3) Scanning electron microscope automatically takes pictures and stores the images (5) Qualitative and quantitative analysis of diatoms: using manual identification Check, classify and statistically process the diatoms in the captured field of view images by means of automatic recognition by computer. The invention has high detection sensitivity, can effectively avoid pollution, is simple, efficient, environmentally friendly, and has accurate qualitative and quantitative analysis, greatly improves the working environment of forensic diatom inspection technicians, reduces labor intensity, and has good application in the practice of forensic drowning diagnosis prospect.
Owner:GUANGZHOU CRIMINAL SCI & TECH RES INST

Integrated dry-wet anaerobic digestion method

The invention discloses an integrated dry-wet anaerobic digestion method; a dry anaerobic area, a three-phase separation area and a wet anaerobic area are arranged in a digestion tank respectively from bottom to top, the dry anaerobic area is provided with a stirrer that provides stirring for material, large-particle-size organic material is degraded into small-particle-size material in the dry anaerobic area, with mass methane bubbles generated, large-particle-size solid material is returned from the three-phase separation area to the dry anaerobic area, small-particle-size solid material and liquid material are fed into the wet anaerobic area, the wet anaerobic area is provided with a methane aerating pipe, the upper portion of the wet anaerobic area is provided with an upper discharge pipe, water overflows from the upper discharge pipe, methane produced by anaerobic digestion enters a top gas chamber above the wet anaerobic area, and the top of the top gas chamber is provided with a top methane pipe. An integrated dry-wet anaerobic digestion apparatus provided herein is high in organics degradation rate, high in methane production, low in residue production and good in effluent quality, is available for automatic continuous methane production from high-concentration organic solid waste, and is suitable particularly for the anaerobic digestive production of low-water-content high-impurity-content organic materials.
Owner:SHANGHAI MUNICIPAL ENG DESIGN INST GRP

Method and device for quickly and accurately resolving and measuring total nitrogen with portable pressure cooker combined with colorimetric tubes

The invention belongs to the technical field of environmental protection, and relates to a method and a device for quickly and accurately resolving and measuring total nitrogen with a portable pressure cooker combined with colorimetric tubes. According to the method and the device, the colorimetric tubes can be vertically and uniformly distributed in the portable pressure cooker, so that samples can sufficiently contact with high temperature steam; sufficient digestion of potassium persulfate is achieved, so that the problem of excessive white light absorption caused by incomplete digestion ofthe potassium persulfate is solved; the method and the device can also fix the plugs of the colorimetric tubes, so that the measurement error caused by loosening or ejection of the plugs of the colorimetric tubes in the high-temperature and high-pressure environment is prevented; the invention takes full advantage of the existing pressure cooker and the colorimetric tubes in the total nitrogen measuring labor, so that idle and waste of resources are not incurred; the problem of excessive white light absorption caused by incomplete digestion can be prevented to the greatest extent; in this way, a lot of manpower and material resources in the exploration process of the operator can be saved; and the invention has an important meaning in total nitrogen measurement.
Owner:HUNAN UNIV

An integrated wet and dry anaerobic digestion method

The invention discloses an integrated dry-wet anaerobic digestion method. A dry anaerobic zone, a three-phase separation zone and a wet anaerobic zone are respectively arranged inside the digestion tank from bottom to top; the dry anaerobic zone is provided with stirring The agitator realizes the material stirring, and the organic material with a large particle size is degraded into a small-size material in the dry anaerobic zone, and a large amount of biogas bubbles are generated at the same time; the solid-phase material with a large particle size returns to the dry-type anaerobic zone through the three-phase separation zone. anaerobic zone, while the small particle size and liquid phase enter the wet anaerobic zone; the wet anaerobic zone is provided with a biogas aeration pipe, and the upper part of the wet anaerobic zone is provided with an upper discharge pipe, and the upper discharge pipe is used to overflow The way the water is discharged, the biogas produced by anaerobic enters the top air chamber above the wet anaerobic zone, and the top biogas pipe is set on the top of the top air chamber. The integrated dry-wet anaerobic digestion device provided by the present invention has high organic matter degradation rate, high biogas output, low biogas residue output, and good effluent quality, which can realize automatic and continuous biogas production of high-concentration organic solid waste, and is especially suitable for low moisture content and impurities. Anaerobic digestion of organic materials with high content.
Owner:SHANGHAI MUNICIPAL ENG DESIGN INST GRP
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