Diatom inspection method in medical jurisprudence

Abstract

The invention relates to the field of medical jurisprudence inspection, in particular to a diatom inspection method in medical jurisprudence. The method at least comprises the following steps: (1) microwave digestion: adding concentrated nitric acid and hydrogen peroxide solution into the inspected sample to carry out microwave digestion; (2) vacuum filtration: carrying out vacuum filtration on the digestion solution, adding ultrapure water, carrying out vacuum filtration continually until the surface of the filter membrane is approximately neutral, adding absolute ethyl alcohol, and carrying out vacuum filtration to remove moisture from the filter membrane; (3) a scanning electron microscope automatically takes pictures and stores the pictures; and (4) qualitative and quantitative analysis on diatom: carrying out inspection, classification and statistic treatment on the diatom in the on-spot pictures in an artificial identification way or computer automated identification way. The invention has the advantages of high detection sensitivity, high accuracy of qualitative and quantitative analysis, simpleness, high efficiency and environmental protection, can effectively avoid pollution, greatly improves the working environment for diatom inspection technicians in medical jurisprudence, and reduces the labor intensity, thereby having wide application prospects in drowning diagnosis practice in medical jurisprudence.

Description

technical field [0001] The invention relates to the field of forensic medical inspection, in particular to a forensic medical diatom inspection method for identifying the cause of death of corpses in water by inspecting the diatom species and content in organs, tissues and organs. Background technique [0002] The cause of death analysis of corpses in water has always been one of the difficulties in the practice of forensic medicine. Since the 1940s, many forensic scientists have confirmed on the bodies of the drowned that diatoms can enter various organs of the large circulation. Although there are still differences in the diagnostic value of diatom detection in theory, in forensic practice, diatom detection is still considered to be a relatively reliable means of diagnosing drowning, especially for the identification of the cause of death of decayed corpses in water. good identification method. Under the condition that pollution can be prevented, more diatoms of the same...

AI Technical Summary

Problems solved by technology

[0004] The traditional strong acid digestion method is open. During the digestion process, there is a danger of strong acid spraying out and burning the human body. In addition, a large amount of strong acid and corrupted test materials in forensic practice release nitrogen dioxide and malodorous gas during the digestion process, which not only pollutes the environment Moreover, the working environment of the forensic examiners is abnormally bad; and the traditional strong acid digestion method generally takes repeated centrifugation and then makes a film for observation. The repeated centrifugation process will lead to the loss of diatoms. Experiments show that centrifugation once leads to about 10% Diatom loss, increased centrifugation speed does not significantly improve diatom recovery, thus resulting in low recovery of diatom extraction from visceral tissue

[0005] At present, most forensic laboratories only use optical microscopes for qualitative and quantitative analysis of diatoms in digested tissues. However, optical microscope analysis is intensive and limited by the magnification. For micro diatoms, it is easy to miss or cannot accurately identify them species

Images