68results about How to "Digest thoroughly" patented technology

The invention discloses a measuring method for quickly measuring the contents of heavy metals in tobacco by using a microwave digestion / ICP-MS (Inductively Coupled Plasma Mass Spectrometry) method. According to the measuring method, a microwave digestion pretreatment method is adopted, an ICP-MS is adopted to measure the contents of arsenic, plumbum, cadmium, chromium, copper, zinc, nickel and mercury elements in a digestion liquid, 75As, 208Pb, 111Cd, 53Cr, 63Cu, 66Zn, 60Ni and 202Hg are selected as mass numbers of the to-be-measured elements to reduce mass spectrum interference, and an internal standard element Rh is added in an off-line manner to inhibit the matrix effect. For to-be-measured ions with a certain mass-to-charge ratio, mass spectrum signal response is directly proportional to the number of ions entering the ICP-MS, the concentration of the elements in a sample is measured by measuring the number of mass spectrum signals, and the contents of the elements are calculated by a standard curve method. The method is quick and simple, can efficiently and accurately measure the contents of heavy metals in tobacco and tobacco products, and provides an effective scheme for finding out the content level of heavy metals in cigarettes in China to reduce harm to human health.

The invention discloses an isolated culture method of human amnia mesenchymal stem cells, which comprises the following steps: carrying out human placenta amnia tissue acquisition, amnia tissue disinfection, washing, shredding and mixed collagenase digestion to obtain mesenchymal stem cells, and carrying out mesenchymal stem cell in-vitro culture. The method can obtain abundant mesenchymal stem cells, which have the advantages of uniform and stable phenotype and high multiplication capacity and have the multidirectional differentiation potential, from the human amnia tissues; and the culture medium in-vitro amplification is performed to purify the cells, thereby satisfying the quantity for cryopreservation or clinical use. The method has the advantages of short cell culture period and high repetitiveness. The stem cells obtained by the method have the phenotypes of CD105<+>, CD90<+> and CD73<+>.

The invention relates to the field of medical jurisprudence inspection, in particular to a diatom inspection method in medical jurisprudence. The method at least comprises the following steps: (1) microwave digestion: adding concentrated nitric acid and hydrogen peroxide solution into the inspected sample to carry out microwave digestion; (2) vacuum filtration: carrying out vacuum filtration on the digestion solution, adding ultrapure water, carrying out vacuum filtration continually until the surface of the filter membrane is approximately neutral, adding absolute ethyl alcohol, and carrying out vacuum filtration to remove moisture from the filter membrane; (3) a scanning electron microscope automatically takes pictures and stores the pictures; and (4) qualitative and quantitative analysis on diatom: carrying out inspection, classification and statistic treatment on the diatom in the on-spot pictures in an artificial identification way or computer automated identification way. The invention has the advantages of high detection sensitivity, high accuracy of qualitative and quantitative analysis, simpleness, high efficiency and environmental protection, can effectively avoid pollution, greatly improves the working environment for diatom inspection technicians in medical jurisprudence, and reduces the labor intensity, thereby having wide application prospects in drowning diagnosis practice in medical jurisprudence.

The invention relates to a low-consumption and environment-protection method for extracting chitin and bioactive substances from shrimp shell, which comprises the steps that: the fresh shrimp shell is arranged in a microwave oven for heating, then is arranged in the NaOH solution and boiled for 1 to 2 hours, then is immersed in the HCI solution for immersion; the shrimp shell is treated again with the NaOH solution, and is arranged in the sunshine and heavily sunned for decoloration after immersed in the HCI solution, thus the chitin product is obtained; when protein powder is extracted, protein is separated from the alkali treatment solution through the physical process of freezing and thawing; and when astaxanthin product is extracted, papain is adopted for degradation, then astaxanthin is obtained through ethanol extraction. The invention has the advantages of high material utilization ratio of shrimp shell, less acid and alkali consumption, less dosage of organic solvent, complete separation of various useful substances in shrimp shell, high purity of the obtained product, no discharge of waste acid, waste alkali and harmful organic solvent during reaction process, and effective use of various treatment solutions generated during reaction.

The invention discloses a sample processing device for soil heavy metal detection and a processing method thereof. The processing device comprises a hopper, a screen-out device, a preprocessing device, an allocation device, a sample rack and a digestion device. The screen-out device comprises a separating base, a positive electrode suction device, a screen-out fan and a collecting groove. The preprocessing device comprises a drying chamber, an electric heating wire, a lifting crushing screen and a sample suction port. The suction pump of the allocation device is connected with the sample suction port through a pipeline. The allocation device is connected with a metering valve. The sample rack comprises a rotating base used for rotating the sample rack at a fixed angle. A plurality of sample tubes are circumferentially arranged on the rotating base. An ultrasonic generator is arranged at the connection between the sample tubes and the rotating base. The digestion device comprises a storage tank and a digestion liquid peristaltic pump. In a word, the sample processing device has the advantage of novel structure, perfect method, good processing effect, small detection error and the like.

The invention belongs to the technical field of insect feed additives, and particularly discloses an insect additive for poultry and livestock feeds and application of the insect additive. The additive comprises the following components in part by weight: 20 to 50 parts of insect albumen powder, and 1 part of chitin and antimicrobial peptide. The chitin and the antimicrobial peptide which are extracted from insects are used for compounding the additive for the feeds, and the quality of the feed is improved, so that the quality of raised animals is effectively improved, and safe and healthy meat and fish or egg products are produced.

The invention discloses a method for detecting chromium, nickel, arsenic, selenium, cadmium and lead elements in cigarette ashes. The method is characterized in that cigarette ashes are collected by using a cigarette ash plate, a cigarette ash sample is dispelled by adopting a microwave digestion method, and the contents of chromium, nickel, arsenic, selenium, cadmium and lead elements in the cigarette ashes are detected by inductively coupled plasma mass spectrometry. The cigarette ashes are collected by adopting the cigarette ash plate, independent collection of each cigarette ash is achieved on a conventional smoking machine, and loss of cigarette ashes and cross contamination of different cigarette ashes in the smoking process are effectively avoided. The sample is processed by adopting the microwave digestion method, so that the risk caused by use of a perchloric acid in the traditional electric hot plate digestion mode is avoided, the dosage of the acid is reduced, the digestion ability is improved, the method is good in digestion effect, less in element loss, and simple and easy to operate, and the used mixed acid system is applicable to treatment of the cigarette ash sample, and thorough to digest. The interference is effectively corrected by adopting inductively coupled plasma mass spectrum parameters, and the method is high in sensitivity, good in repeatability, high in recovery rate, and accurate in result.

The invention relates to a material analysis method, in particular to an alloy chemical analysis method. A dissolution method of a high carbon ferrochrome sample comprises the following steps: firstly, placing the high carbon ferrochrome sample to be measured into a microwave digestion tank, then adding a mixture of concentrated hydrochloric acid, concentrated nitric acid and hydrofluoric acid, and finally sealing the microwave digestion tank and putting into a microwave digestor for dissolution, wherein, process parameters obtained during the dissolution in the microwave digestor are as follows: the power is 400-1600w, the temperature is increased from normal temperature to 195 DEG C and the digestion time is 65-110min. 10-25mL of the mixture of the concentrated hydrochloric acid, the concentrated nitric acid and the hydrofluoric acid are needed when 0.0500-0.2000g of the high carbon ferrochrome sample is added. As microwave digestion method is adopted in the alloy chemical analysis method, the microwave activation characteristic causes the sample to be dissolved more completely and the sample can be dissolved without high-temperature fuming or fusion.

The invention provides a decomposing treatment method for medical garbage which which mainly comprises the following steps, first, loading the medical garbage into a degradation apparatus, charging oxygenizing bactericidal agent and amendment, then carrying out warming, pressurizing germicidal treatment at the temperature of 150-280 deg. C, the pressure of 0.2-1.2 MPa, finally opening the discharge port of the degradation apparatus, so as to discharge the residue out of the degradation apparatus for sorting treatment.

The invention provides a procuring and separating culture method of human adipose-derived stem cells and a construction method of a pool of stem cells. The procuring and separating culture method comprises the following steps: collecting human fat tissues; treating the human fat tissues; digesting human fat stem cells; and culturing the human fat stem cells. The construction method of the pool ofstem cells comprises the following steps: procuring, separating and culturing the human fat stem cells; numbering the procured human fat stem cells with IDs, recording and procuring donor informationof the human fat stem cells, and linking the ID numbers to the donor information to form a cell information file and storing the cell information file in a database to form the human fat pool of stemcells. The procuring and separating culture method of human adipose-derived stem cells, provided by the invention, not only is more thorough in fat tissue effect, but also is short in culture time. Inthe culture process, a special culture medium can realize quicker cell proliferation, the purity of stem cells is higher, and in the cell culture process, stem cell differentiation is avoided, and inthe cell cryopreservation process, the survival rate is higher.

The invention discloses a nitrogen blowing instrument. The nitrogen blowing instrument comprises a bracket, a shell on which a pipeline is placed, a blowing needle and a heating device, wherein the shell with the pipeline is fixed on the upper part of the bracket, the heating device is fixed at the lower part of the bracket, the lower surface of the shell with the pipeline is provided with the detachable blowing needle, the blowing needle is connected with the pipeline, and the pipeline and the blowing needle are made from polytetrafluoroethylene and copolymer thereof. The invention further discloses a wet digestion method using the nitrogen blowing instrument. The method comprises the following steps: 1) placing a sample and a reaction reagent in a digestion cup; 2) placing the digestion cup on the heating device of the nitrogen blowing instrument; 3) placing the blowing needle of the nitrogen blowing instrument in the digestion cup and introducing a protection gas; and 4) setting or changing the heating temperature of the heating device according to a preset digestion temperature according to the types of the sample and the reaction reagent. The nitrogen blowing instrument disclosed by the invention is simple in structure, and is capable of effectively preventing explosion when applied to wet digestion, the security of the operation is improved, the secondary pollution of metal can be effectively avoided, and the accuracy of the detection result is guaranteed.

The invention relates to a method for detecting the contents of chromium, nickel, arsenic, selenium, cadmium and lead in a cigarette butt, which is characterized in that a cigarette butt collecting method is specified; a cigarette butt sample is decomposed by using micro-wave; and the contents of chromium, nickel, arsenic, selenium, cadmium and lead in the cigarette butt are detected through the inductively coupled plasma mass spectrometry. According to the invention, the disadvantages of the cigarette butt collecting method in the existing detection technology are overcome; the cigarette butt collecting method is perfect; the cigarette butt sample is decomposed by using micro-wave, therefore, the use amount of acid and the decomposition time are effectively reduced; the method disclosed by the invention is good in decomposing effect, little in element loss and simple to operate; in a mixed acid decomposing system, the cigarette butt sample is thoroughly decomposed; decomposing solution is clear and transparent and free from precipitation; parameters of the inductively coupled plasma mass spectrometry are adopted; the matrix effect is effectively reduced; and the method disclosed by the invention is high in sensitivity, good in repeatability, high in recovery rate and accurate in result.

The invention provides a set of fermented Chinese herbal medicine composition for non-antibiotic breeding of live pigs and a preparation method for the fermented Chinese herbal medicine composition. According to the Chinese herbal medicine composition, through tonifying qi, nourishing blood, nourishing yin and tonifying yang, healthy qi is compromised and complemented, and healthy energy is strengthened; pathogenic factors are guided and eliminated through sweating, purgation and toxin counteracting, and diseases are prevented from happening and developing, so that all fights in a body stop, and a harmonious condition is achieved. The healthy level of a sow is nursed from a source sow, so that newborn piglets are healthy in this way. Combined with health nursing of piglets, nursery pigs and fattening pigs, the piglets live through a conservation period safely, health of the middle pigs is ensured, and growth of fattening pigs is promoted. Occurrence of diseases in all growing periods of the live pigs is prevented, so that the purposes that pig herd is healthy and no antibiotic is added in the whole breeding process are achieved. At the same time, the Chinese herbal medicine composition can further promote the digestion and absorption efficiency of the live pigs to feed, feed conversion rate is improved, the economical benefit is improved, the effects of non-antibiotic breeding, ecological breeding, and economical breeding are achieved truly.

The invention relates to an M-preferred monosaccharide exo-algin lyase Aly-6 as well as a coding gene and application thereof. An amino acid sequence of the M-preferred monosaccharide exo-algin lyaseAly-6 is shown in SEQ ID NO. 2; and a nucleotide sequence for encoding the Aly-6 is shown in SEQ ID NO. 1. The invention discloses for the first time that an M-preferred monosaccharide exo-algin lyaseAly-6 is obtained from a genome of the flammeovirga yaeyamensis MY04. The enzyme is a monosaccharide exo-algin lyase and has an obvious M-preferred feature; the enzyme Aly-6 can continuously degradethe guluronic acid fragment from the algin in a monosaccharide excision manner, and can also continuously degrade the mannuronic acid fragment in a monosaccharide excision manner; and the enzyme Aly-6is easier to degrade the mannosolic acid fragment. The sequence characteristics, substrate degradation pattern and oligosaccharide formation characteristics of the enzyme are significantly differentfrom the sequence characteristics, substrate degradation pattern and oligosaccharide formation characteristics of the existing known algin lyase. The physical and chemical properties are stable, the activity is high, and the potential for industrial application is appreciable.

The invention discloses a method for microwave digestion of a glass body. The method comprises the following steps that 1, a glass body sample is put in a digestion tank, ultrapure water is added to make the glass body sample immersed in the ultrapure water, nitric acid, perchloric acid, hydrofluoric acid and ultrapure water are added in sequence, and the digestion tank is tightly closed and is put in a microwave digestion instrument for heating; 2, the digestion tank is taken out and cooled to room temperature, a digestion tank cover is opened, the ultrapure water is added, and the digestion tank is tightly closed and is put in the microwave digestion instrument for heating again; 3, the step 2 is repeated; 4, the digestion tank is taken out and cooled to room temperature, and the heavy metal content of a digestion solution is detected. The method determines metals of the glass body, achieves accurately determination of trace elements, reduces the requirement of a digestion device, adopts microwaves to digest the sample and performs direct determination through instruments without independent acid catching. The method has the advantages of including simple steps, making sample digestion complete and being good in practicability and low in reagent consumption, and meanwhile the sensitivity and accuracy of element analysis are improved.

The invention discloses a pretreatment method for detecting metal elements in kapok powder. The pretreatment method comprises the steps that 1, kapok is crushed and then put into a digestion tank, and nitric acid and hydrogen peroxide are added into the digestion tank; 2, the digestion tank is put into a digestion instrument for microwave digestion; 3, digestion sample liquid in the digestion tank is transferred into a polytetrafluoroethylene crucible, the digestion tank is washed with ultrapure water, all washing liquid is gathered into the polytetrafluoroethylene crucible, and heating is performed for removing the acid; 4, when the polytetrafluoroethylene crucible is cooled to room temperature, the liquid in the polytetrafluoroethylene crucible is all transferred into a volumetric flask, the polytetrafluoroethylene crucible is washed with the ultrapure water, and all the washing liquid is gathered into the volumetric flask, and constant volume is performed with nitric acid with the mass fraction of 2%. Compared with traditional open type methods such as an ashing method and an acid heating digestion method, the pretreatment method has the advantages of being short in time consumption, little in pollution and reagent amount consumption, thorough in digestion and good in reproducibility, and the problem that organic substances left in sample decomposition disturb detection can be effectively solved.

The invention discloses a method for performing a hot frowsty treatment on steel slag by applying a steel slag frowsty pot. The method includes the steps that 1, the slag is filled, a cover is sealed,a slag bag with the solid hot steel slag is placed in a pot body, locked, and sealed; 2, exhaust gas hot frowsty is performed, the hot frowsty is peformed first, that is, water is sprayed to generatea large amount of steam; then voltage stability control is carried out for the first time; then a second hot frowsty is carried out, and the exhaust gas containing the two components of SO2 and CO2 is introduced into the slag bag; and then a secondary voltage stability control is carried out; 3, replacement and decompression are carried out, low-temperature inert gas is introduced into the pot body, and an exhaust port is opened to perform air exchange in the pot body until the concentrations of combustible gas and residual exhaust gas in the pot body are both lower than a set value; then aninert gas introduction device is closed, and exhaust is continued until pressure difference between the inside and outside of the pot body is zero; and 4, the slag is discharged, the water is drained,that is, the end cover is opened, the slag bag is taken out, and draining is carried out. According to the method, the exhaust gas containing SO2 and CO2 is utilized for decomposition, and the decomposition is more thorough.

The invention discloses a method for separating and culturing trigeminal root ganglion neurons of a mammal in vitro and application of the method, and belongs to the technical field of cell biology and neurobiology. The method comprises the following steps: placing the trigeminal root ganglion neurons of the mammal in a culture dish containing L-15 culture solution for washing treatment, cutting the trigeminal root ganglion neurons into pieces and then carrying out digestion treatment with type 11 collagenase, trypsin, and trypsin-EDTA in sequence, filtering with a cell strainer and then carrying out density gradient centrifugation for the filtrate, suspending and inoculating the obtained product to a wrapped cell culture device, and culturing with a basic neuronal medium. The method greatly reduces the damage to the neurons in the tissue processing process, removes the impurity contamination in the digestion process like cell debris and other cells, greatly improves the purity of the neurons, can obtain a million of pure trigeminal ganglion neurons, improves the yield and the quality of the trigeminal ganglion neurons, and is suitable for the in-vitro separation and culturing of the trigeminal root ganglion neurons of the mammal.

The invention provides a gastrointestinal dynamic simulation device. The device comprises a stomach simulation cavity, an intestine simulation cavity, a blood simulation cavity, a liquid storage device and samplers. A medicine basket in which medicines can be put is arranged in the stomach simulation cavity, and artificial gastric juice and artificial intestinal juice can be injected into the cavity. A simulated intestinal wall is attached and embedded between the blood simulation cavity and the intestine simulation cavity, and PH regulating liquid can be injected into the intestine simulationcavity. Human tissue fluid or simulated blood can be injected into the blood simulation cavity. Media in the cavities are sampled through the samplers on the stomach simulation cavity and the blood simulation cavity. The device is simple in structure and convenient to use, a dissolution medium for simulating artificial gastric juice and intestinal juice is added, medicine absorption testing is carried out by simulating the intestinal wall and sampling under the action of an air bag and a stirrer, in-vitro and in-vivo correlation is greatly improved, and the device can be used for predicting in-vitro release and in-vivo absorption of solid oral preparations.

The invention relates to a method for accurately measuring the selenium content in wine. The method comprises the following steps of (1) pretreating the wine: heating a wine sample to be measured in water bath, adding a nitric acid solution and hydrogen peroxide for carrying out cold digestion, carrying out heating digestion after staying overnight until the solution is clarified, and adding hydrochloric acid for reduction to obtain a treated sample; (2) adopting a hydride generation-atomic fluorescence spectroscopy to measure a standard selenium solution and the treated sample obtained in the step (1); using a fluorescence signal to quantify, and drawing a standard work curve to quantitatively analyzing the sample to be measured; calculating to obtain the total selenium content of the sample to be measured according to a formula (1): X=(C-C0) X V / v (1), wherein X is the selenium content (mu g / L) in the sample to be measured, C is the selenium concentration (mu g / L) in the treated sample, C0 is the selenium concentration (mu.g / L) of the sample through blank determination, V is the total volume (mL) of the measured liquid, and v is the sample weighting volume (mL). The method is moderate, more thorough in digesting organic matters, less in loss, non-toxic, non-pollution, simple and easy to operate, good in repeatability, and high in accuracy.

The invention provides a coal chemical wastewater biological treatment system based on sludge catalytic ozonation and a treatment method thereof. The system comprises an anoxic / aerobic biological treatment system for first-grade predenitrification, a sludge catalytic ozonation multiphase reaction system and a second-grade anoxic denitrification / aerobic biological treatment system. The method comprises the following steps: subjecting to-be-treated coal chemical wastewater to anoxic / aerobic biological treatment of first-grade predenitrification, subjecting one part of treated sludge to refuxing and the other part of the treated sludge to ozone catalytic digestion, mixing a supernatant generated by ozone catalytic digestion with the sewage obtained by anoxic / aerobic biological treatment of first-grade predenitrification, and subjecting the obtained mixture to second-grade anoxic denitrification / aerobic biological treatment so as to obtain effluent water, wherein tail gas generated by catalytic digestion is used for aeration treatment for second-grade anoxic denitrification / aerobic biological treatment. According to the invention, a sludge catalytic ozonation multiphase reactor is adopted, so highly-efficient and low-cost oxidation digestion of the sludge can be realized, and functions of a novel method are guaranteed to be realized.

The invention discloses a method for quick detection of contents of microelements in xuefuzhuyu pills. A microwave digestion pretreatment method is adopted, a flame atomic absorption spectrometer is used to measure contents of copper, zinc, iron, magnesium and calcium in digestion liquid, and the degree of selective absorption of light of characteristic spectral lines by to-be-measured metal elements is proportional to the concentrations of the elements in a solution within a certain range. By measurement of absorbance of ground state atoms of the elements under the selected spectral lines, the concentrations of the elements in a sample are measured, and a standard curve method is adopted to measure the contents of the elements. According to the method, the contents of the microelements in the xuefuzhuyu pills can be detected quickly, simply, efficiently and accurately, and a certain basis is provided for discussion of a relationship between the microelements and functions of the xuefuzhuyu pills.

The invention discloses a determination method for the lead content in polydimethylsiloxane and emulsion thereof. At a pre-treatment phase, a sample is prepared by adopting a method of performing microwave digestion for mixed acid dissolution of hydrofluoric acid and nitric acid and the sample is determined by a graphite furnace atomic absorption spectrophotometer; meanwhile, two types of matrix modifiers including palladium nitrate and diammonium phosphate are added. The lead content in the polydimethylsiloxane and the emulsion thereof can be rapidly and accurately determined. Compared with traditional dry-process digestion pre-treatment and no hydrofluoric acid, the determination method has the advantages of less sampling amount, short reaction time, complete digestion, less loss of elements to be determined and easiness for operation. By adopting the determination method, after characteristics of the polydimethylsiloxane and the emulsion thereof are determined by adopting optimized related temperature raising procedure, charring temperature and atomization temperature of a graphite furnace atomic absorption spectrometry, the two types of matrix modifiers are used for eliminating interference; a recycling rate is high and a determined result is accurate.

The invention discloses a detection method of lead content of food. The method comprises the following steps: (1) pretreating a sample; (2) preparing a blank solution and a standard solution; and (3) detecting lead content. By the detection method, food is completely degraded, acid consumption is low, and content of lead in food can be rapidly and accurately detected in batches.

The invention discloses an adipose-derived stem cell filler. The adipose-derived stem cell filler comprises adipose-derived stem cells, fibro stem cells, fibroblast and nutrients for cell growing, wherein the densities of the adipose-derived stem cells, the fibro stem cells and the fibroblast are 1*10<7> to 5*10<7> per ml, 0.5*10<7> to 1*10<7> per ml and 0.5*10<7> to 1*10<7> per ml respectively, and the concentration of the nutrients for cell growing is 20*30 mg / ml. The invention further discloses a preparation method of the adipose-derived stem cell filler. The filler provided by the invention can improve defective tissue repairing quality through the synergistic effect of the adipose-derived stem cells, the fibro stem cells, the fibroblast and the nutrients.

The invention relates to a process for preparing a fertilizer product by resourceful treatment of fermentation waste residues of tetracyclic antibiotics. The process comprises the following steps: (1)heating, stirring, activating, size mixing and dispersing the fermentation waste residues containing tetracyclic antibiotics to obtain waste residue slurry; (2) adding a digestion agent and a coupling agent into the waste residue slurry, carrying out a digestion reaction, and carrying out solid-liquid separation after the reaction is completed to respectively obtain a recyclable solid raw material and a filtrate; (3) carrying out substance recycling on the filtrate part, wherein high-temperature energy generated in the digestion reaction process can be returned to the step (1) to realize energy recycling; and (4) blending, stirring and granulating the recyclable solid raw material and auxiliary materials to obtain the fertilizer product. Compared with the prior art, the process has the advantages that antibiotic residues and resistance genes in the tetracyclic antibiotic fungi residues can be effectively removed, the environmental risk is reduced, in addition, filtrate recycling and energy recycling are achieved, energy is saved, consumption is reduced, three-waste pollution is effectively reduced, and green cyclic development is achieved.

The invention discloses a dental pulp stem cell separation and culture method. The method comprises steps as follows: separation of dental pulp tissue, enzymolysis of the dental pulp tissue with IV type collagenase, V type collagenase and pancreatin mixed enzyme with the mass volume concentration of 0.05%-0.5%, primary culture of dental pulp stem cells and adding of cell growth factors, glutamine,decavitamin, insulin, tremella polysaccharide, a codonopsis tangshen extract, a dendrobium officinale extract and other nutritional ingredients. The method has the advantages as follows: the dental pulp tissue is more thoroughly digested, and the total number of the obtained dental pulp stem cells is larger; the total number of the obtained dental pulp stem cells is increased, the cell viabilityof the dental pulp stem cells is increased, besides, the passage time of the stem cells is shortened, and the isolated culture cycle of dental pulp stem cells is greatly shortened.

The invention provides a traditional Chinese medicine composition for treating a cad pig and a preparation method thereof. According to the traditional Chinese medicine composition, a traditional Chinese medicine composition 1 has the efficacy of relieving exterior syndrome by diaphoresis, resolving the flesh and dispelling the wind, invigorating pulse-beat, supporting Yang and resolving Qi, and the like; a traditional Chinese medicine composition 2 has the efficacy of clearing away heat and toxic materials, cooling blood and purging intense heat, and the like; a traditional Chinese medicine composition 3 has the efficacy of warming middle-Jiao to dispel cold, tonifying Qi and nourishing spleen, promoting transportation and transformation, and the like; aiming at the qi-blood deficiency type cad pig and the Qi-stagnancy and blood stasis type cad pig, the whole formula adopts therapeutic principles of tonifying middle-Jiao and Qi, drying the spleen to promote the stomach, promoting flow of qi and blood and attacking accumulation to urge congestion according to the holism concept and the differential treatment of the Chinese veterinarian theory; treatment aiming at the pathogenesis and the symptom is carried out on a cad inducing factor, and the growth and development of pigs are promoted, a damaged organ can be quickly repaired, no drug residue exists, recrudescence is avoided, the immunocompetence of the swinery is enhanced and the self-repairing capability and adaptive capacity to environment are realized.

The invention discloses a method for preparing an antioxidative peptide liquid of collagen gel with combination of ultrasonic treatment and simulative digestion and belongs to the technical field of food processing technology and protein utilization. According to the adopted preparation process, the collagen gel is prepared from pepsin-soluble collagen taken as a raw material through dissolution, dialysis, ultrasonic treatment and self-assembly under a water bath, an in-vitro simulative gastrointestinal digestion technology is used, a bionic dynamic rat gastric digestion system capable of simulating peristole frequency and amplitude and simulating a secretory gland to secrete gastric digestive juice is used, then intestinal digestion is simulated, and two-step enzyme digestion is adopted by means of pepsin-trypsin. The enzyme degradation rate is increased greatly, and the rate of utilization of the raw material is increased. A dynamic model is used for simulating gastrointestinal digestion processes and is closer to a human body simulative digestion environment in an environment at 37 DEG C, the process is more efficient, and the method is safer and more reliable as compared with an artificial synthesis method and a fermentation method for antioxidative peptides.