Adipose-derived stem cell filler and preparation method thereof

Abstract

The invention discloses an adipose-derived stem cell filler. The adipose-derived stem cell filler comprises adipose-derived stem cells, fibro stem cells, fibroblast and nutrients for cell growing, wherein the densities of the adipose-derived stem cells, the fibro stem cells and the fibroblast are 1*10<7> to 5*10<7> per ml, 0.5*10<7> to 1*10<7> per ml and 0.5*10<7> to 1*10<7> per ml respectively, and the concentration of the nutrients for cell growing is 20*30 mg / ml. The invention further discloses a preparation method of the adipose-derived stem cell filler. The filler provided by the invention can improve defective tissue repairing quality through the synergistic effect of the adipose-derived stem cells, the fibro stem cells, the fibroblast and the nutrients.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to an adipose stem cell filler and a preparation method thereof. Background technique

[0002] Human tissue is usually damaged due to aging and trauma. For example, with the increase of age, the signs of aging are gradually obvious, and wrinkles and depressions of different degrees will appear on the face due to the loss of fat, which will affect the beauty of the face. At present, fillers are usually used to repair defective tissues, and the fillers are mainly paraffin, silica gel, animal collagen, polyacrylamide gel, hyaluronic acid gel, cell growth factors, and the like.

[0003] However, paraffin, silica gel, animal collagen, and polyacrylamide gel are prone to rejection due to non-human body substances. Hyaluronic acid gel is decomposed and absorbed due to metabolism, which cannot achieve the purpose of long-term filling. The time of staying is quite short, and the purpose of stim...

Examples

Embodiment 1

[0018] The adipose stem cell filler includes adipose stem cells, fibroblast stem cells, fibroblasts, and cell growth nutrients, wherein the densities of adipose stem cells, fibroblast stem cells, and fibroblasts are 1×10 7 -5×10 7 pcs / ml, 0.5×10 7 -1×10 7 pcs / ml, 0.5×10 7 -1×10 7 cells / ml, the concentration of cell growth nutrients is 20-30mg / ml.

[0019] In this example, the densities of adipose stem cells, fibroblasts, and fibroblasts were 3×10 7 pcs / ml, 0.8×10 7 pcs / ml, 0.8×10 7 cells / ml, the concentration of cell growth nutrients is 25mg / ml.

[0020] In this embodiment, the preparation method of the fat stem cell filler includes the following steps: fully dissolving the cell growth nutrient in water, adding fat stem cells, fibroblast stem cells, and fibroblasts, and resuspending each cell to prepare the fat stem cell filler.

[0021] Among them, in the process of obtaining the fat stem cells, the method of digestion with mixed collagenase is used to obtain them. M...

Embodiment 2

[0035] Adipose stem cell fillers include adipose stem cells, fibroblast stem cells, fibroblasts, and cell growth nutrients, wherein the densities of adipose stem cells, fibroblast stem cells, and fibroblasts are 2×10 7 pcs / ml, 0.6×10 7 pcs / ml, 0.6×10 7 cells / ml, the concentration of cell growth nutrients is 25mg / ml.

[0036] In this embodiment, the preparation method of the fat stem cell filler includes the following steps: fully dissolving the cell growth nutrient in water, adding fat stem cells, fibroblast stem cells, and fibroblasts, and resuspending each cell to prepare the fat stem cell filler.

[0037] Among them, in the process of obtaining the fat stem cells, the method of digestion with mixed collagenase is used to obtain them. Mixed collagenase is composed of type Ⅱ collagenase and papain, and its concentration is 0.1% (g / ml) when digesting adipose tissue. The preparation method of mixed collagenase 1% (g / ml) mother solution is as follows: add 0.5g type II collage...

technical field

[0001] The invention relates to the field of biotechnology, in particular to an adipose stem cell filler and a preparation method thereof. Background technique

[0002] Human tissue is usually damaged due to aging and trauma. For example, with the increase of age, the signs of aging are gradually obvious, and wrinkles and depressions of different degrees will appear on the face due to the loss of fat, which will affect the beauty of the face. At present, fillers are usually used to repair defective tissues, and the fillers are mainly paraffin, silica gel, animal collagen, polyacrylamide gel, hyaluronic acid gel, cell growth factors, and the like.

[0003] However, paraffin, silica gel, animal collagen, and polyacrylamide gel are prone to rejection due to non-human body substances. Hyaluronic acid gel is decomposed and absorbed due to metabolism, which cannot achieve the purpose of long-term filling. The time of staying is quite short, and the purpose of stim...

Examples

Embodiment 1

[0018] The adipose stem cell filler includes adipose stem cells, fibroblast stem cells, fibroblasts, and cell growth nutrients, wherein the densities of adipose stem cells, fibroblast stem cells, and fibroblasts are 1×10 7 -5×10 7 pcs / ml, 0.5×10 7 -1×10 7 pcs / ml, 0.5×10 7 -1×10 7 cells / ml, the concentration of cell growth nutrients is 20-30mg / ml.

[0019] In this example, the densities of adipose stem cells, fibroblasts, and fibroblasts were 3×10 7 pcs / ml, 0.8×10 7 pcs / ml, 0.8×10 7 cells / ml, the concentration of cell growth nutrients is 25mg / ml.

[0020] In this embodiment, the preparation method of the fat stem cell filler includes the following steps: fully dissolving the cell growth nutrient in water, adding fat stem cells, fibroblast stem cells, and fibroblasts, and resuspending each cell to prepare the fat stem cell filler.

[0021] Among them, in the process of obtaining the fat stem cells, the method of digestion with mixed collagenase is used to obtain them. M...

Embodiment 2

[0035] Adipose stem cell fillers include adipose stem cells, fibroblast stem cells, fibroblasts, and cell growth nutrients, wherein the densities of adipose stem cells, fibroblast stem cells, and fibroblasts are 2×10 7 pcs / ml, 0.6×10 7 pcs / ml, 0.6×10 7 cells / ml, the concentration of cell growth nutrients is 25mg / ml.

[0036] In this embodiment, the preparation method of the fat stem cell filler includes the following steps: fully dissolving the cell growth nutrient in water, adding fat stem cells, fibroblast stem cells, and fibroblasts, and resuspending each cell to prepare the fat stem cell filler.

[0037] Among them, in the process of obtaining the fat stem cells, the method of digestion with mixed collagenase is used to obtain them. Mixed collagenase is composed of type Ⅱ collagenase and papain, and its concentration is 0.1% (g / ml) when digesting adipose tissue. The preparation method of mixed collagenase 1% (g / ml) mother solution is as follows: add 0.5g type II collage...