Method for accurately measuring selenium content in wine

A technology for accurate determination and content, applied in the field of analytical chemistry, can solve problems such as equipment or personnel damage, and achieve the effects of high accuracy, good repeatability, and simple and easy operation.

Inactive Publication Date: 2016-12-21
哈尔滨中科龙祥科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for the detection of total selenium in wine, many methods in China do not mention that nitric acid and ethanol will react under heating conditions (CH 3 CH 2 OH+HNO 3 →CH 3 CH 2 -O-NO 2 +H 2 O), the severity of this reaction may cause damage to equipment or personnel

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  • Method for accurately measuring selenium content in wine
  • Method for accurately measuring selenium content in wine
  • Method for accurately measuring selenium content in wine

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Determination of total selenium content in embodiment 1 selenium-enriched ganoderma lucidum wine

[0027] The steps of the pretreatment method for the determination of total selenium content in selenium-enriched Ganoderma lucidum wine were optimized. The optimization process and results are as follows:

[0028] Measure 50 mL of wine sample into a conical flask, heat it in a water bath at 80°C for 1 to 2 hours, and take the end point of the water bath when the wine smell is basically gone, add 8 mL of nitric acid, shake well and let it stand for 30 minutes, then add 2 mL of hydrogen peroxide drop by drop, every Drop 0.5mL, shake well after each drop and let stand for 2min, cold digest for 6h, heat digest at 80°C for 30min, heat digest at 90°C for 30min, heat digest at 100°C for 1-2h until clarification, add 5mL after cooling After reducing with hydrochloric acid for 3 hours, transfer the solution in the Erlenmeyer flask to a 25mL colorimetric tube for testing.

[0029] ...

example 2

[0039] Determination of total selenium content in example 2 selenium-enriched wheat wine

[0040] The implementation conditions are the same as those in Example 1, and Table 4 and Table 5 are the measurement results of total selenium in selenium-enriched wheat wine and the measurement results of standard addition recovery respectively.

[0041] Table 4 Determination results of total selenium in wine samples

[0042]

[0043]

[0044] Table 5 The recovery and determination results of selenium-enriched wheat wine

[0045]

[0046] It can be seen from Table 5 that the recovery rate of the addition of selenium-enriched wheat wine is 79% to 88%, and the recovery effect is ideal, indicating that the pretreatment method and determination method are reliable.

[0047] To sum up, the present invention can fully digest the organic matter in the wine and fully reduce the selenium-containing compounds, so as to facilitate the detection of the total selenium content. This is of ...

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Abstract

The invention relates to a method for accurately measuring the selenium content in wine. The method comprises the following steps of (1) pretreating the wine: heating a wine sample to be measured in water bath, adding a nitric acid solution and hydrogen peroxide for carrying out cold digestion, carrying out heating digestion after staying overnight until the solution is clarified, and adding hydrochloric acid for reduction to obtain a treated sample; (2) adopting a hydride generation-atomic fluorescence spectroscopy to measure a standard selenium solution and the treated sample obtained in the step (1); using a fluorescence signal to quantify, and drawing a standard work curve to quantitatively analyzing the sample to be measured; calculating to obtain the total selenium content of the sample to be measured according to a formula (1): X=(C-C0) X V / v (1), wherein X is the selenium content (mu g / L) in the sample to be measured, C is the selenium concentration (mu g / L) in the treated sample, C0 is the selenium concentration (mu.g / L) of the sample through blank determination, V is the total volume (mL) of the measured liquid, and v is the sample weighting volume (mL). The method is moderate, more thorough in digesting organic matters, less in loss, non-toxic, non-pollution, simple and easy to operate, good in repeatability, and high in accuracy.

Description

technical field [0001] The invention relates to a quantitative determination method in the field of analytical chemistry, in particular to a method for accurately determining the selenium content in wine. Background technique [0002] Selenium is one of the essential trace elements in the human body. Since Schwartz discovered that Se has a nutritional effect in 1957, other functions of Se have also been unearthed. Se, as an essential component of glutathione peroxidase (GSH-Px) in the human body, has the functions of anti-oxidation and eliminating free radicals, so as to delay aging and improve immunity. In daily life, people's demand for wine accounts for a large proportion. At the same time, with the rise of health wine, selenium-enriched wine also has a certain market prospect. When producing selenium-enriched wine, it is also necessary to measure the selenium content in it, so as to judge whether the selenium intake can enable consumers to reach the recommended dietary ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6404G01N2021/6417
Inventor 王张民
Owner 哈尔滨中科龙祥科技有限公司
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