35 results about "Tissue acquisition" patented technology

Systems, devices, and methods for planning an endoscopic tissue acquisition procedure for acquiring tissue from an anatomical target are disclosed. An endoscopic system comprises a steerable elongate instrument and a processor. The steerable elongate instrument can be positioned and navigated in a patient anatomy and acquire tissue from an anatomical target via a biopsy tool associated with the steerable elongate instrument. The processor can receive an image of the anatomical target, apply the received image to a trained machine-learning (ML) model to determine a tissue acquisition plan that includes a recommended biopsy tool and operational parameters for navigating the steerable elongate instrument or maneuvering the recommended biopsy tool. The tissue acquisition plan can be presented to a user, or used to facilitate a robot-assisted tissue acquisition procedure.

The present disclosure provides methods of amplifying tissue samples derived from humans for purposes of treating certain diseases. The method comprises: isolating a small amount of associated tissue by biopsy or other tissue acquisition methods; the tissue is amplified in vitro, and meanwhile, natural functions are reserved; and re-implanting the amplified tissue to alleviate human disease. One example includes ex vivo expansion of human pancreatic islet tissue and reimplantation of the expanded cells into an individual with Type 1 diabetes for treatment and long term relief or cure of disease.

The invention discloses an adipose tissue obtaining needle capable of reducing bleeding and nerve injury, which comprises a guide rod, one end of the guide rod is fixedly communicated with a negative pressure suction bottle connecting assembly, the other end of the guide rod is fixedly communicated with a needle head, and the guide rod is provided with a plurality of water drop holes close to the needle head. A user cannot be stabbed when being accidentally touched, the sharp and solid round blunt needle penetrates through a collection part to reduce the resistance of a traditional blunt end, the phenomenon that the cortex of the collection part is damaged is avoided, and the strain of the collection part is reduced; the water drop holes are formed, stress is better dispersed, the tensile strength of the guide rod during fat collection is improved, the service life is prolonged, and compared with a traditional solid guide rod and a guide rod with the water drop holes, normal use is guaranteed, meanwhile, the weight of the guide rod is reduced, and positioning and transferring are convenient; and by arranging the guide groove, the problems that the needle deviates from the fat collecting direction, fat extraction is not uniform, and even the fat extraction position deviates are solved.

The application provides a kind of based on digestive tract tissue protein expression abundance determination method and system, applied to digestive tract tissue image processing technical field. Including: based on the PVDF membrane image after color development of digestive tract tissue acquisition in vitro, contain at least one protein standard band and the protein band of multiple target proteins;When determining the presence of electrophoresis distortion according to the band of target protein standard, construct pixel space coordinate system;The target image is obtained by angle correction and linear repair to PVDF membrane image, and the protein band corresponding to multiple target proteins in target image is in horizontal alignment state under pixel space coordinate system;Determine the development area of each target protein in target image and the irregular region of interest wrapped the protein band corresponding to multiple target proteins, and for each target protein, the expression abundance of target protein is obtained by analyzing the development area and the irregular region of interest, and the accuracy of the protein expression abundance is higher.

A tissue closure system includes an endoscope having first and second lumens therethrough, first and second control elements disposed within the first and second lumens, respectively, and a tissue engaging portion integral with a distal end of the endoscope. The tissue engaging portion includes a tissue acquisition assembly and a tissue closure assembly. The tissue acquisition assembly is controllable via the first control element, and the tissue closure assembly controllable via the second control element. The tissue engaging portion includes a tissue grasping portion for engaging first and second target tissue portions disposed adjacent an opening in target tissue. The tissue closure assembly is configured to pierce the target tissue, and to hold the first and second target tissue portions together to close the opening in the target tissue.

The invention relates to the technical field of biomedicine, in particular to a method for identifying fibroblast subsets in colorectal cancer tissue based on single cell sequencing, and provides a method for identifying fibroblast subsets in colorectal cancer tissue based on single cell sequencing. The method comprises the following steps: S1, extracting colorectal cancer tissues: obtaining tumor tissues removed from the colorectal cancer tissues, and collecting a suspension to prepare a single-cell suspension; s2, single cell sequencing analysis: constructing a high-throughput sequencing library; s3, data analysis: processing the sequencing data by using a CellRanger software pipeline, and mapping readings to a genome and a transcriptome; and carrying out data dimension reduction and visualization. S4, identification of the tumor-associated fibroblast subpopulation: after the total cell type in the colorectal cancer tumor tissue is identified, annotating the cells; and identifying differentially expressed genes by using a Seurt software package, and carrying out function enrichment analysis, so as to identify various cell types.

A method includes inserting an introducer needle into a tissue to be biopsied. A biopsy sample is obtained through the introducer needle from the tissue and the tissue proximate a tip of the introducer needle is ablated by an ablation antenna without removing the introducer needle. When removing the introducer needle from the tissue, the method may include coagulating blood in or cauterizing an insertion path in the tissue with the ablation antenna as the introducer needle is removed from the tissue. The insertion path is formed by the insertion of the introducer needle into the tissue.

The invention provides a holder, a target tissue component detection system and a target tissue component detection method. The target tissue component detection method comprises the steps that the clamping structure clamps a target tissue, and the active heating unit heats the target tissue; the first real-time temperature of the active heating unit and the second real-time temperature, detected by the passive temperature measuring unit, of the target tissue are obtained; determining a real-time temperature difference between the first real-time temperature and the second real-time temperature; generating an actual temperature difference characteristic curve of real-time temperature difference related to time; determining a plurality of first key point parameters of the actual temperature difference characteristic curve; determining a plurality of second key point parameters on each preset temperature difference characteristic curve based on the preset temperature difference characteristic curves of the plurality of different component sample tissues in the storage unit; and matching the plurality of first key point parameters with the plurality of second key point parameters, and determining a target tissue component. By determining the real-time temperature difference between the active heating unit and the target tissues, the target tissues of different types and different components can be identified and distinguished in real time.

A phantom-free calibration method for a computerized tomography scan has the steps of: scanning a plurality of homogeneous human tissues with a computerized tomography scanner and obtaining homogeneous human tissue scan information; scanning a tissue to be tested with the computerized tomography scanner and obtaining tissue to be tested scan information; calculating a spectral characteristic parameter of the computerized tomography scanner with a computing device using a model, wherein a standard tissue parameter of the homogeneous human tissues and the human tissue scan information are used in the model; and calculating a tissue parameter of the tissue to be tested with the computing device using the model, wherein the spectral characteristic parameter and the tissue to be tested scan information are inputted in the model. The phantom-free calibration method makes the tissue parameters of the tissue to be tested more accurate and the calibration process easier and more convenient.

The invention relates to a living body capillary dynamic nerve imaging tracking method, system and device and a medium, and the method comprises the steps: collecting the continuous capillary image data of a living body tissue; performing blood vessel segmentation processing on the continuous capillary image data, and identifying a capillary network skeleton for motion analysis; based on the capillary network skeleton, calculating a motion vector of the segmented continuous capillary image data, and obtaining a motion track of red blood cells in the capillary; according to the motion trail, haemodynamic parameters including the flow velocity, the direction and the blood oxygen level are generated; synchronously acquiring local neural activity data of the continuous capillary image data under the same space-time coordinate system; and according to the haemodynamic parameters and the local neural activity data, generating a tracking result of the in-vivo microvascular haemodynamics and neural activity, so as to achieve the purpose of performing synchronous tracking and dynamic correlation analysis on the microvascular haemodynamics and neural activity in the in-vivo environment.

Systems, devices, and methods for planning endoscopic ultrasound (EUS)-guided tissue acquisition (EUS-TA) from an anatomical target are disclosed. An endoscopy system comprises a steerable elongate instrument including an EUS probe to produce ultrasound scans of the anatomical target, and a tissue acquisition device to sample tissue. A processor receives image of the anatomical target including one or more EUS images converted from the ultrasound scans, apply the images to a trained machine-learning model to generate an EUS-TA plan. The EUS-TA plan may include a recommended tissue acquisition device, and recommended values of operational parameters for manipulating the tissue acquisition device, navigating the steerable elongate instrument, or positioning the EUS probe. The EUS-TA plan can be presented to a user, or used to facilitate a robot-assisted tissue acquisition procedure.

The present application relates to a kind of stitching device and suture system.Suture device includes: first base, first base is provided with first channel through its length direction, the bottom of first base is provided with needle hole with the far end of first channel communication;Tissue acquisition structure below first base, tissue acquisition structure is configured to grasp or release the human tissue to be sutured;Rotary drive structure, rotary drive structure is arranged in first base, the output end of rotary drive structure extends the far end of first base;Needle structure, needle structure includes suture, needle holder and with the detachable insertion of needle assembly one end of needle holder, the other end of needle holder is connected with the output end of rotary drive structure, needle holder and needle assembly insertion one end is pointed, suture is connected with needle assembly;And limiting structure, limiting structure is arranged in first channel, limiting structure is configured to fix or loosen needle assembly.

A medical imaging system and method for detecting temporal changes in biological tissue through coherent change detection of complex-valued microwave signals. Unlike conventional microwave imaging systems that acquire single-timepoint data, the disclosed system maintains phase coherence between temporally separated measurements using dedicated real-time calibration channels that continuously monitor and correct phase drift in the signal path, as well as physical and algorithmic registration and compensation methods. The system acquires magnitude and phase backscatter data from tissue at multiple timepoints, applies spatial registration to compensate for patient repositioning, and computes coherent differences from the registered complex data to detect changes in tissue dielectric and conductivity properties below the spatial resolution limit of single-timepoint imaging. The coherent change detection method enables identification of tissue changes and growth with sensitivity exceeding conventional temporal subtraction techniques. Applications include breast cancer screening and monitoring, intracranial hemorrhage detection, and lung cancer screening and monitoring.

Systems, devices, and methods for planning endoscopic ultrasound (EUS)-guided tissue acquisition (EUS-TA) from an anatomical target are disclosed. An endoscopy system can comprise a steerable elongate instrument including an EUS probe to produce ultrasound scans of the anatomical target, and a tissue acquisition device to sample tissue. A processor can receive one or more images of the anatomical target including one or more EUS images converted from the ultrasound scans, and apply the one or more images to a trained machine-learning model to generate an EUS-TA plan. The EUS-TA plan may include a recommended tissue acquisition device, and recommended values of operational parameters for manipulating the tissue acquisition device, navigating the steerable elongate instrument, or positioning the EUS probe. The EUS-TA plan can be presented to a user, or used to facilitate a robot-assisted tissue acquisition procedure.

The invention discloses an oral instrument, an information generation method and system thereof and a storage medium, and the method comprises the steps: determining a target intraoral tissue according to target instrument type information, obtaining at least one group of original oral data at least pointing to the target intraoral tissue according to the target intraoral tissue, and obtaining at least one group of original oral data at least pointing to the target intraoral tissue according to the original oral data; the distribution condition and / or occupation condition of the target intraoral tissue in the inner cavity space dimension are / is analyzed, at least one group of oral cavity feature data is obtained, and according to the target instrument type, oral cavity instrument information closest to the oral cavity feature data in the space dimension is determined in a target instrument information base through retrieval according to the oral cavity feature data. According to the oral instrument information generation method provided by the invention, the dependence of selection errors and clinical experience can be avoided, and the matching degree between the oral instrument information and the model or the actual condition is improved.

According to the technical scheme, the bionic hand system comprises a hand tissue obtaining module used for obtaining internal structure characteristics of the hand to obtain internal data; data acquisition is carried out on external and internal structural characteristics and forms of a human hand, a hand bone model is printed and manufactured according to the acquired data, then the hand bone works in a control driving mode, and different required grabbing postures are completed by utilizing different preset posture data, so that the hand bone model is printed and manufactured. The structure and the function of a human hand are highly simulated, so that the advantage of flexibility of the bionic hand is improved, the bionic dexterous hand which is highly anthropomorphic is constructed, the complex structure and the function of the human hand are simulated, the bionic dexterous hand adapts to a complex and changeable operation environment, and the bionic dexterous hand is realized through deep theoretical analysis, precise mechanical design and a precise control algorithm. A bionic hand prototype with high flexibility and adaptability is successfully constructed.

The present disclosure provides methods of expanding tissue samples derived from a human being for the purpose of treating certain diseases. The methods include isolation of a small amount of the relevant tissue through biopsy or other methods of tissue acquisition, expanding such tissues ex vivo while retaining natural functioning, and reimplantation of the expanded tissues to alleviating human disease. One example includes ex vivo expansion of human pancreatic islet tissue and reimplantation of the expanded cells into an individual with Type 1 Diabetes for the treatment and long term remission or cure of the disease.

The invention relates to the technical field of immunofluorescent staining, in particular to an adipose tissue whole-tissue immunofluorescent staining method based on tabletting treatment. According to the method, tissue acquisition and fixation, tissue tabletting, membrane rupture and sealing, degreasing and autofluorescence removal, fluorescent staining, sheet sealing and imaging are sequentially carried out. According to the invention, a complete tabletting-degreasing-fluorescent staining integrated process is established, a set of systematic process which is standardized, high in repeatability and adaptive to conventional experimental conditions is provided, and tabletting whole tissue staining is realized in adipose tissues for the first time. The core of the invention lies in that the thickness of the tissue is reduced by a tabletting treatment means, and a set of optimized antibody permeation, sealing, degreasing and anti-autofluorescence treatment flow is matched, so that the immunofluorescent staining of the adipose tissue is effectively realized on the premise of not depending on complex tissue transparency and special imaging equipment, and the immunofluorescent staining effect is greatly improved. The method is especially suitable for detecting deep or low-abundance targets such as nerve markers.

This invention provides a surgical field tracking and adjustment method and a surgical robot system. The surgical field tracking and adjustment method includes the following steps: acquiring preliminary information based on CT images of the surgical area; identifying tissue in the surgical field of the endoscope using machine vision; and acquiring the coordinates of the lesion tissue and the end effector of the surgical instrument in a reference coordinate system. A motion vector is determined; inverse kinematics calculations are performed based on the motion vector; and a control signal is output to drive the first robotic arm to move, thereby causing a preset object to appear at a preset position in the surgical field; wherein the preset object includes the lesion tissue and / or the end effector of the surgical instrument. This configuration, through automatic adjustment of the surgical field using an algorithm, reduces the requirements for operators and improves surgical efficiency, solving the problems of high barriers to entry, low efficiency, and long time associated with manual intervention in surgical field adjustment in existing technologies.

Systems, devices, and methods for planning an endoscopic tissue acquisition procedure for acquiring tissue from an anatomical target are disclosed. An endoscopic system comprises a steerable elongate instrument and a processor. The steerable elongate instrument can be positioned and navigated in a patient anatomy and acquire tissue from an anatomical target via a biopsy tool associated with the steerable elongate instrument. The processor can receive an image of the anatomical target, apply the received image to a trained machine-learning (ML) model to determine a tissue acquisition plan that includes a recommended biopsy tool and operational parameters for navigating the steerable elongate instrument or maneuvering the recommended biopsy tool. The tissue acquisition plan can be presented to a user, or used to facilitate a robot-assisted tissue acquisition procedure.

The present disclosure relates to the field of endoscopy. Specifically, the present disclosure relates to systems and methods for real-time visualization and sampling of target tissue within body passages. In particular, the present disclosure relates to a system that provides real-time visualization of eccentric pulmonary nodules, and which allows the location / orientation of a biopsy needle to be determined prior to its first actuation.

An aspect of the present application provides a computer-executed method for generating an image of a simulated development or treatment result of a dental condition, comprising: acquiring a first image obtained by means of superimposing a mask on a second image, the second image showing dental-related tissue of a patient; acquiring a simulation category, which represents a development or treatment result of a dental condition to be simulated; and using a trained deep neural network to generate a third image on the basis of the first image and the simulation category, a region of the third image corresponding to the mask being a simulated development or treatment result of the dental condition.

The invention discloses a method for culturing Tibetan sheep muscle cells. Relates to the technical field of cell separation culture. Comprising the following steps: tissue acquisition; cleaning and pretreating tissues; cutting and digesting tissues; collecting cells; purifying the cells; culturing cells; and changing the bottle. The cell adherence is early, the laying is fast, and the culture period is obviously shortened. The viability of the cultured cells reaches 95% or above, the cells are uniform in shape and are in a typical muscle cell fusiform or strip shape, and follow-up research and application are facilitated. And the purification effect is good: impurities such as fibroblasts and the like are effectively removed through the steps of digestion optimization and bottle replacement, and the cell purity is high. The repeatability is high, the operation process is standardized, key parameters are clear, and the consistent culture effect can be obtained among different laboratories.

A core needle biopsy device includes a body, a needle assembly and a drive assembly. The needle assembly extends distally from the body and includes a piercer, a hollow outer cannula, and a cutting element. The outer cannula defines an internal lumen. The piercer is disposed within the outer cannula. The cutting element extends along an outer surface or an inner surface of the outer cannula. The cutting element is configured to translate relative to the outer cannula. The drive assembly is configured to selectively move one or more of the piercer, the outer cannula, and the cutting element. The drive assembly is further configured to move the piercer relative to the outer cannula to open and close the internal lumen defined by the outer cannula.

The invention discloses a secondary harmonic imaging method for analyzing a depression model mouse living body hippocampus collagen structure, and belongs to the technical field of neuropathology and tissue imaging, and the method comprises the following steps: (1) sample preparation: tissue acquisition and pretreatment, and sample fixation and mounting; (2) unmarked two-photon imaging and image acquisition, including imaging system configuration and image acquisition parameter setting; (3) image analysis: a second harmonic imaging technology is adopted to directly capture inherent signals of collagenous fibers through a nonlinear optical effect, and a three-dimensional reconstruction algorithm is combined, so that the following breakthroughs are realized: unmarked imaging: a coloring agent or an antibody is not needed, and signal interference is avoided; performing high-resolution three-dimensional analysis: obtaining the spatial orientation and density distribution of collagenous fibers through two-photon laser layer scanning and Z-axis stacking; the method is used for accurately analyzing the collagen structure in the brain sea horse tissue of the depression model mouse, and overcomes the defects in three-dimensional presentation and resolution in the prior art.

The present application relates to a kind of suturing device.The suturing device includes: base, base is provided with first passage, second passage and third passage through its length direction;Suture needle assembly, suture needle assembly includes suture needle, suture needle is U-shaped, one end of U-shaped is in first passage, the other end is pointed and opposite to third passage, suture needle assembly can be extended relative to base;Tissue acquisition assembly, tissue acquisition assembly is in second passage and can be extended relative to base, tissue acquisition assembly is configured to grasp or release the human tissue to be sutured;Suture assembly, suture assembly includes suture and suture joint connected with suture, suture joint includes first end and second end, first end is used for with suture needle detachable insertion;And needle joint assembly, needle joint assembly is set in third passage and can be extended relative to base, needle joint assembly is configured to fix or loosen the second end of suture joint.

An apparatus for measuring a blood flow parameter in a blood vessel, including: an interferometric data collection apparatus including a light source and a sensor coupled to a controller, the controller being configured to: direct the light source toward a proximal side of a blood vessel; obtain interferometric data from a tissue adjacent to and outside of a distal side of the blood vessel opposite the proximal side; determine a signal modulation rate based on the interferometric data; and estimate a blood flow parameter in the blood vessel based on the signal modulation rate.

Systems, devices, and methods for determining a sampling device for use in an ultrasound-guided endoscopic tissue acquisition procedure are disclosed. An exemplary system includes an ultrasound imaging device to generate an ultrasound image of an anatomical target in a real-time ultrasound field of view (FOV); a display for displaying an ultrasound image in the ultrasound FOV; and a processor. Based on a position of the anatomical target in the ultrasound FOV, the processor predicts a nominal sampling device trajectory intersecting the anatomical target from a plurality of nominal sampling device trajectories. The plurality of nominal trajectories correspond to different hardware configurations of the plurality of nominal sampling devices. The processor determines a sampling device recommendation having a hardware configuration from the predicted nominal sampling device trajectory.

The application discloses a kind of biological partial tissue acquisition device and method, biological partial tissue acquisition device includes outer catheter, inner catheter and at least one cutting edge, inner catheter is movably nested in outer catheter, and at least one gap part is opened on the outer wall of inner catheter;Cutting edge is preformed into set shape, and the first end of cutting edge is set in gap part, and cutting edge can be switched between storage state and expansion state;When cutting edge is in storage state, cutting edge is housed in the inner cavity of outer catheter with inner catheter, and the second end of cutting edge is coplanar with the outer wall of inner catheter;When cutting edge is in expansion state, cutting edge restores to set shape, and the second end of cutting edge protrudes from the outer wall of inner catheter.The biological partial tissue acquisition device can sample more biological samples with smaller diameter inner catheter, thereby improving the use effect of biological partial tissue acquisition device.