19 results about "Tissue acquisition" patented technology

The present disclosure provides methods of amplifying tissue samples derived from humans for purposes of treating certain diseases. The method comprises: isolating a small amount of associated tissue by biopsy or other tissue acquisition methods; the tissue is amplified in vitro, and meanwhile, natural functions are reserved; and re-implanting the amplified tissue to alleviate human disease. One example includes ex vivo expansion of human pancreatic islet tissue and reimplantation of the expanded cells into an individual with Type 1 diabetes for treatment and long term relief or cure of disease.

The invention discloses an adipose tissue obtaining needle capable of reducing bleeding and nerve injury, which comprises a guide rod, one end of the guide rod is fixedly communicated with a negative pressure suction bottle connecting assembly, the other end of the guide rod is fixedly communicated with a needle head, and the guide rod is provided with a plurality of water drop holes close to the needle head. A user cannot be stabbed when being accidentally touched, the sharp and solid round blunt needle penetrates through a collection part to reduce the resistance of a traditional blunt end, the phenomenon that the cortex of the collection part is damaged is avoided, and the strain of the collection part is reduced; the water drop holes are formed, stress is better dispersed, the tensile strength of the guide rod during fat collection is improved, the service life is prolonged, and compared with a traditional solid guide rod and a guide rod with the water drop holes, normal use is guaranteed, meanwhile, the weight of the guide rod is reduced, and positioning and transferring are convenient; and by arranging the guide groove, the problems that the needle deviates from the fat collecting direction, fat extraction is not uniform, and even the fat extraction position deviates are solved.

The application provides a kind of based on digestive tract tissue protein expression abundance determination method and system, applied to digestive tract tissue image processing technical field. Including: based on the PVDF membrane image after color development of digestive tract tissue acquisition in vitro, contain at least one protein standard band and the protein band of multiple target proteins;When determining the presence of electrophoresis distortion according to the band of target protein standard, construct pixel space coordinate system;The target image is obtained by angle correction and linear repair to PVDF membrane image, and the protein band corresponding to multiple target proteins in target image is in horizontal alignment state under pixel space coordinate system;Determine the development area of each target protein in target image and the irregular region of interest wrapped the protein band corresponding to multiple target proteins, and for each target protein, the expression abundance of target protein is obtained by analyzing the development area and the irregular region of interest, and the accuracy of the protein expression abundance is higher.

A tissue closure system includes an endoscope having first and second lumens therethrough, first and second control elements disposed within the first and second lumens, respectively, and a tissue engaging portion integral with a distal end of the endoscope. The tissue engaging portion includes a tissue acquisition assembly and a tissue closure assembly. The tissue acquisition assembly is controllable via the first control element, and the tissue closure assembly controllable via the second control element. The tissue engaging portion includes a tissue grasping portion for engaging first and second target tissue portions disposed adjacent an opening in target tissue. The tissue closure assembly is configured to pierce the target tissue, and to hold the first and second target tissue portions together to close the opening in the target tissue.

The invention relates to the technical field of biomedicine, in particular to a method for identifying fibroblast subsets in colorectal cancer tissue based on single cell sequencing, and provides a method for identifying fibroblast subsets in colorectal cancer tissue based on single cell sequencing. The method comprises the following steps: S1, extracting colorectal cancer tissues: obtaining tumor tissues removed from the colorectal cancer tissues, and collecting a suspension to prepare a single-cell suspension; s2, single cell sequencing analysis: constructing a high-throughput sequencing library; s3, data analysis: processing the sequencing data by using a CellRanger software pipeline, and mapping readings to a genome and a transcriptome; and carrying out data dimension reduction and visualization. S4, identification of the tumor-associated fibroblast subpopulation: after the total cell type in the colorectal cancer tumor tissue is identified, annotating the cells; and identifying differentially expressed genes by using a Seurt software package, and carrying out function enrichment analysis, so as to identify various cell types.

The invention relates to a living body capillary dynamic nerve imaging tracking method, system and device and a medium, and the method comprises the steps: collecting the continuous capillary image data of a living body tissue; performing blood vessel segmentation processing on the continuous capillary image data, and identifying a capillary network skeleton for motion analysis; based on the capillary network skeleton, calculating a motion vector of the segmented continuous capillary image data, and obtaining a motion track of red blood cells in the capillary; according to the motion trail, haemodynamic parameters including the flow velocity, the direction and the blood oxygen level are generated; synchronously acquiring local neural activity data of the continuous capillary image data under the same space-time coordinate system; and according to the haemodynamic parameters and the local neural activity data, generating a tracking result of the in-vivo microvascular haemodynamics and neural activity, so as to achieve the purpose of performing synchronous tracking and dynamic correlation analysis on the microvascular haemodynamics and neural activity in the in-vivo environment.

Systems, devices, and methods for planning endoscopic ultrasound (EUS)-guided tissue acquisition (EUS-TA) from an anatomical target are disclosed. An endoscopy system comprises a steerable elongate instrument including an EUS probe to produce ultrasound scans of the anatomical target, and a tissue acquisition device to sample tissue. A processor receives image of the anatomical target including one or more EUS images converted from the ultrasound scans, apply the images to a trained machine-learning model to generate an EUS-TA plan. The EUS-TA plan may include a recommended tissue acquisition device, and recommended values of operational parameters for manipulating the tissue acquisition device, navigating the steerable elongate instrument, or positioning the EUS probe. The EUS-TA plan can be presented to a user, or used to facilitate a robot-assisted tissue acquisition procedure.

The present application relates to a kind of stitching device and suture system.Suture device includes: first base, first base is provided with first channel through its length direction, the bottom of first base is provided with needle hole with the far end of first channel communication;Tissue acquisition structure below first base, tissue acquisition structure is configured to grasp or release the human tissue to be sutured;Rotary drive structure, rotary drive structure is arranged in first base, the output end of rotary drive structure extends the far end of first base;Needle structure, needle structure includes suture, needle holder and with the detachable insertion of needle assembly one end of needle holder, the other end of needle holder is connected with the output end of rotary drive structure, needle holder and needle assembly insertion one end is pointed, suture is connected with needle assembly;And limiting structure, limiting structure is arranged in first channel, limiting structure is configured to fix or loosen needle assembly.

A medical imaging system and method for detecting temporal changes in biological tissue through coherent change detection of complex-valued microwave signals. Unlike conventional microwave imaging systems that acquire single-timepoint data, the disclosed system maintains phase coherence between temporally separated measurements using dedicated real-time calibration channels that continuously monitor and correct phase drift in the signal path, as well as physical and algorithmic registration and compensation methods. The system acquires magnitude and phase backscatter data from tissue at multiple timepoints, applies spatial registration to compensate for patient repositioning, and computes coherent differences from the registered complex data to detect changes in tissue dielectric and conductivity properties below the spatial resolution limit of single-timepoint imaging. The coherent change detection method enables identification of tissue changes and growth with sensitivity exceeding conventional temporal subtraction techniques. Applications include breast cancer screening and monitoring, intracranial hemorrhage detection, and lung cancer screening and monitoring.

Systems, devices, and methods for planning endoscopic ultrasound (EUS)-guided tissue acquisition (EUS-TA) from an anatomical target are disclosed. An endoscopy system can comprise a steerable elongate instrument including an EUS probe to produce ultrasound scans of the anatomical target, and a tissue acquisition device to sample tissue. A processor can receive one or more images of the anatomical target including one or more EUS images converted from the ultrasound scans, and apply the one or more images to a trained machine-learning model to generate an EUS-TA plan. The EUS-TA plan may include a recommended tissue acquisition device, and recommended values of operational parameters for manipulating the tissue acquisition device, navigating the steerable elongate instrument, or positioning the EUS probe. The EUS-TA plan can be presented to a user, or used to facilitate a robot-assisted tissue acquisition procedure.

The invention relates to the technical field of immunofluorescent staining, in particular to an adipose tissue whole-tissue immunofluorescent staining method based on tabletting treatment. According to the method, tissue acquisition and fixation, tissue tabletting, membrane rupture and sealing, degreasing and autofluorescence removal, fluorescent staining, sheet sealing and imaging are sequentially carried out. According to the invention, a complete tabletting-degreasing-fluorescent staining integrated process is established, a set of systematic process which is standardized, high in repeatability and adaptive to conventional experimental conditions is provided, and tabletting whole tissue staining is realized in adipose tissues for the first time. The core of the invention lies in that the thickness of the tissue is reduced by a tabletting treatment means, and a set of optimized antibody permeation, sealing, degreasing and anti-autofluorescence treatment flow is matched, so that the immunofluorescent staining of the adipose tissue is effectively realized on the premise of not depending on complex tissue transparency and special imaging equipment, and the immunofluorescent staining effect is greatly improved. The method is especially suitable for detecting deep or low-abundance targets such as nerve markers.

The present application relates to a kind of suturing device.The suturing device includes: base, base is provided with first passage, second passage and third passage through its length direction;Suture needle assembly, suture needle assembly includes suture needle, suture needle is U-shaped, one end of U-shaped is in first passage, the other end is pointed and opposite to third passage, suture needle assembly can be extended relative to base;Tissue acquisition assembly, tissue acquisition assembly is in second passage and can be extended relative to base, tissue acquisition assembly is configured to grasp or release the human tissue to be sutured;Suture assembly, suture assembly includes suture and suture joint connected with suture, suture joint includes first end and second end, first end is used for with suture needle detachable insertion;And needle joint assembly, needle joint assembly is set in third passage and can be extended relative to base, needle joint assembly is configured to fix or loosen the second end of suture joint.

An apparatus for measuring a blood flow parameter in a blood vessel, including: an interferometric data collection apparatus including a light source and a sensor coupled to a controller, the controller being configured to: direct the light source toward a proximal side of a blood vessel; obtain interferometric data from a tissue adjacent to and outside of a distal side of the blood vessel opposite the proximal side; determine a signal modulation rate based on the interferometric data; and estimate a blood flow parameter in the blood vessel based on the signal modulation rate.

Systems, devices, and methods for determining a sampling device for use in an ultrasound-guided endoscopic tissue acquisition procedure are disclosed. An exemplary system includes an ultrasound imaging device to generate an ultrasound image of an anatomical target in a real-time ultrasound field of view (FOV); a display for displaying an ultrasound image in the ultrasound FOV; and a processor. Based on a position of the anatomical target in the ultrasound FOV, the processor predicts a nominal sampling device trajectory intersecting the anatomical target from a plurality of nominal sampling device trajectories. The plurality of nominal trajectories correspond to different hardware configurations of the plurality of nominal sampling devices. The processor determines a sampling device recommendation having a hardware configuration from the predicted nominal sampling device trajectory.

The application discloses a kind of biological partial tissue acquisition device and method, biological partial tissue acquisition device includes outer catheter, inner catheter and at least one cutting edge, inner catheter is movably nested in outer catheter, and at least one gap part is opened on the outer wall of inner catheter;Cutting edge is preformed into set shape, and the first end of cutting edge is set in gap part, and cutting edge can be switched between storage state and expansion state;When cutting edge is in storage state, cutting edge is housed in the inner cavity of outer catheter with inner catheter, and the second end of cutting edge is coplanar with the outer wall of inner catheter;When cutting edge is in expansion state, cutting edge restores to set shape, and the second end of cutting edge protrudes from the outer wall of inner catheter.The biological partial tissue acquisition device can sample more biological samples with smaller diameter inner catheter, thereby improving the use effect of biological partial tissue acquisition device.

The application relates to the technical field of biology, and particularly relates to an animal stem cell preparation method. The method comprises the following steps: S1. donor sample pretreatment: selecting healthy and suitable donor animals, selecting corresponding tissue samples according to the types of the donors, selecting fat tissue or bone marrow tissue for mammals, and selecting blastula tissue for poultry; performing sterile treatment on the tissue samples, removing connective tissue, blood vessels and other impurities, washing the tissue samples with normal saline for 3-5 times, cutting the tissue samples into 1-2 mm3 tissue blocks, and reserving the tissue blocks for use; the donor is slightly damaged and has a wide source: the application can select mammalian fat tissue, bone marrow tissue or poultry blastula tissue as the sample, the fat tissue and the blastula tissue are convenient to obtain, the damage to the donor animals is far less than that of the traditional bone marrow puncture and embryo separation method, the application is suitable for mammals and poultry, has high universality, can realize large-scale sample acquisition, and does not need complex experimental equipment, and the operations such as complex enzymolysis, centrifugation and immunomagnetic bead purification are easy to standardize.

Systems, devices, and methods for planning an ultrasound-guided tissue acquisition process are disclosed. An example system includes an ultrasound imaging device for generating an ultrasound image of an anatomical target in a real-time ultrasound field of view (FOV), a display for displaying the ultrasound image, and a controller circuit. Prior to extending the tissue sampling device into the ultrasound FOV, the controller circuit displays a graphical user interface element (UIE) indicating a predicted nominal sampling device trajectory that intersects an anatomical target in the ultrasound FOV. As the tissue sampling device extends into the ultrasound FOV, the controller circuit determines one or more real-time trajectory parameters and updates the UIE. The controller circuit displays the updated UIE to provide visual feedback as to whether the tissue sampling device will intersect the anatomical target.

The present disclosure provides methods of expanding tissue samples derived from a human being for the purpose of treating certain diseases. The methods include isolation of a small amount of the relevant tissue through biopsy or other methods of tissue acquisition, expanding such tissues ex vivo while retaining natural functioning, and reimplantation of the expanded tissues to alleviating human disease. One example includes ex vivo expansion of human pancreatic islet tissue and reimplantation of the expanded cells into an individual with Type 1 Diabetes for the treatment and long term remission or cure of the disease.