121 results about "Total cell" patented technology

The present invention relates, generally, to a method and apparatus for electrowinning metals, and more particularly to a method and apparatus for copper electrowinning using the ferrous/ferric anode reaction. In general, the use of a flow-through anode—coupled with an effective electrolyte circulation system—enables the efficient and cost-effective operation of a copper electrowinning system employing the ferrous/ferric anode reaction at a total cell voltage of less than about 1.5 V and at current densities of greater than about 26 Amps per square foot (about 280 A/m²), and reduces acid mist generation. Furthermore, the use of such a system permits the use of low ferrous iron concentrations and optimized electrolyte flow rates as compared to prior art systems while producing high quality, commercially saleable product (i.e., LME Grade A copper cathode or equivalent), which is advantageous.

The present invention relates to a polyurethane foam dressing material with improved moisturizing rate. More precisely, the present invention relates to a hydrophilic dressing material in which a wound contact layer (10) having a sponge structure composed of multiple open cells (12) and pores (15) passing through the cells (12) is laminated with a protective film (20). The lamination is characteristically performed at 150˜250° C. under a pressure of preferably 0.25˜1 kgf/cm², the moisturizing rate of the wound contact layer (10) is 300˜1200% and the pore area (membrane opening) takes 10˜35% of the total cell area.

The polyurethane foam dressing material of the present invention prevents the invasion of foreign materials, releases absorbed exudation after changing it into water vapor or arresting the absorbed exudation inside the foam to maintain constant moisturizing, has excellent exudation absorption capacity, has a wound healing effect resulting from not adhering to a wound, is easy to change, and enhances the wound healing effect by maintaining optimum moisturizing conditions resulting from the improved moisturizing rate.

A process is provided for growing microflora of the order Thraustochytriales such as Thraustochytrium, Schizochytrium, and mixtures thereof, in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate. In a preferred embodiment, the process produce, microflora having a cell aggregate size useful for the production of food products for use in aquaculture. Further provided is a food product which includes Thraustochytrium, Schizochytrium, and mixture thereof, and a component selected from flaxseed, reapedeed, soybean and avocado meal. Such a food product includes a balance of long chain and short chain omega-3 highly unsaturated fatty acids. A method for increasing omega-3 highly unsaturated fatty acid content of eggs by feeding a feed to poultry. The feed contains the microorganisms of the order Thraustochytriales having a omega-3 highly unsaturated fatty acid content of more than 6.7 percent of total cell dry weight. The microorganisms include strains of Schizochytrium and Thraustochytrium such as ATCC No.(s) 20888 and 20889, and ATCC No.(s) 20890, 20891 and 20892, respectively. Also provided are eggs containing an increased amount of these fatty acids.

The present invention provides a method of efficiently expanding non-adhesive adult stem cells in vitro. More specifically, the present invention provides a method of expanding non-adhesive adult stem cells, comprising culturing non-adhesive adult stem cells in the co-presence of adhesive feeder cells and non-adhesive non-stem cells in serum-free medium, wherein the ratio of non-adhesive adult stem cell count to total cell count in the medium is kept low; non-adhesive adult stem cells obtained by the expansion method and differentiated cells thereof, and a composition comprising them; and a serum-free medium comprising a specified factor, and a kit for its preparation and the like.

The invention discloses a method for simultaneous and efficient amplification of CD<3+>CD<56+>CIK cells and CD<3->CD<56+>NK cells. The method comprises the steps as follows: the concentration of separated PBMC (peripheral blood mononuclear cells) is adjusted by a serum-free medium containing autologous plasma, an Anti-CD16 antibody, IL-2 and IL-15 are added, and then the mixture is transferred into a T175 culture flask for culture; an Anti-CD3 antibody and an Anti-CD137 antibody are added; a serum-free medium containing the autologous plasma, IL-2 and IL-15 is supplemented every two days according to the cell growth condition; the cell concentration is controlled to be about 1.5*10<6>/ml; and after culture is performed for 14-21 days, large quantities of high-purity CD<3+>CD<56+>CIK cells and CD<3->CD<56+>NK cells can be obtained simultaneously, and the total cell quantity can reach an effective value of the cell quantity required for adoptive cellular immunotherapy clinically for tumor. The method for simultaneous and efficient amplification of the CD<3+>CD<56+>CIK cells and the CD<3->CD<56+>NK cells is simple, convenient, effective and high in cell killing activity.

The invention belongs to the technical field of biology and relates to a method for producing a microecological foliar fertilizer by utilizing probiotics and microalgae. The method comprises the following steps of: firstly, culturing to obtain liquid culture prepared by bacillus, lactic acid bacteria and rhodopseudomonas palustris with the cell concentration being 10 billion/ml and saccharomycetes and chlorella with the cell concentration being 2 billion/ml respectively, then preparing liquid culture with four types of probiotics and one type of chlorella, and mixing the liquid cultures according to respective volume ratio to obtain the liquid microecological foliar fertilizer with the total cell concentration being more than 5 billion/ml. The method has the advantages that the used strains and chlorella are all probiotics which can be eaten by human, and are safe and non-toxic; the microecological foliar fertilizer integrates the advantages of promoting the plant growth of the probiotics, the chlorella and metabolites thereof; and the microecological foliar fertilizer is diluted with water by 100 times every 10 days and is sprayed on plant leaves, and the plant yield is increasedby more than 30% in 60 days. The method has an important application value in aspect of improving the yield and the quality of crops such as grain and the like.

The invention discloses a distribution method of PCIs (Physical Cell Identities) in a long-term evolution network. The method comprises the following steps of: firstly, partitioning two respectively continuous PCI spaces in a PCI range, wherein one is used for distributing PICs in a picocell, and the other is used for distributing PCIs in a micro base cell; distributing a PCI space large enough to the micro base cell so that the PCI multiplexing distance in the micro base cell is far enough, which means the multiplication of ring number of enough macrocell clusters forming the micro base by the cell diameter, and the enough large PCI space means that the number of the distributed PCIs is at least equal to the total cell number of the enough macrocell clusters. On the premise that the macrocell clusters and the picocell are ensured to use different PCI spaces, the PCI multiplexing distance of the macrocell is far enough, PCI conflict probability among picocells is lower, and resources are preserved for network expansion.

The invention discloses an establishment method for a total-cell three-dimensional two-phase model of a proton exchange membrane fuel cell. The model construction method comprises the steps of constructing a global conservation equation, constructing a liquid water distribution relevant conservation equation, constructing an electrochemical reaction relevant conservation equation, introducing a catalyst layer agglomerate model and constructing an expression of conservation equation source items. Relevant equations are established according to the steps, and through numerical solving calculation, gas concentration distribution, liquid water volume fraction distribution, membrane water distribution and cell performance in the proton exchange membrane fuel cell under a catalyst particle volume fraction and an electrolyte volume fraction in catalyst layers can be obtained. Most traditional models ignore liquid water in channels or do not consider the influence of gas-liquid phase change inthe channels. According to the model, a liquid water saturation equation is solved in the channels, and the gas-liquid phase change process is considered; and after liquid water distribution in the channels is obtained, the influence of liquid water on gas flowing in the channels is reflected through correction of a viscosity value in a gas momentum equation, and therefore a good data basis is provided for further research.

The present invention relates, generally, to a method and apparatus for electrowinning metals, and more particularly to a method and apparatus for copper electrowinning using the ferrous/ferric anode reaction and a flow-through anode, such as, for example, a dimensionally stable carbon, carbon composite, metal-graphite, or stainless steel anode. In general, the use of a flow-through anode—coupled with an effective electrolyte circulation system—enables the efficient and cost-effective operation of a copper electrowinning system employing the ferrous/ferric anode reaction at a total cell voltage of less than about 1.5 V and at current densities of greater than about 26 Amps per square foot (about 280 A/m2), and reduces acid mist generation. Furthermore, the use of such a system permits the use of low ferrous iron concentrations and optimized electrolyte flow rates as compared to prior art systems while producing high quality, commercially saleable product (i.e., LME Grade A copper cathode or equivalent), which is advantageous.

The invention discloses a culture method and serum-free culture medium composition of NK cells. The serum-free culture medium composition of the NK cells comprises an induction culture medium, a proliferation culture medium and an activation culture medium; the induction culture medium comprises a basal culture medium and an induction component, the proliferation culture medium comprises a basal culture medium and a proliferation culture component, and the activation culture medium comprises a basal culture medium and an activation component. The culture method of the NK cells comprises the step of performing cell culture by sequentially using the induction culture medium, the proliferation culture medium and the activation culture medium in stages. The serum-free culture medium composition of the NK cells comprises the induction culture medium, the proliferation culture medium and the activation culture medium which are designed for the different influence requirements of the induction, proliferation and activation periods of the NK cells, and the cultured NK cells are superior to those cultured through an existing technology on the aspects such as the total cell number, the proliferation speed, the amplification times and the killing activity on tumor cells.

The present invention relates, generally, to a method and apparatus for electrowinning metals, and more particularly to a method and apparatus for copper electrowinning using the ferrous/ferric anode reaction and a flow-through anode, such as, for example, a dimensionally stable carbon, carbon composite, metal-graphite, or stainless steel anode. In general, the use of a flow-through anode—coupled with an effective electrolyte circulation system—enables the efficient and cost-effective operation of a copper electrowinning system employing the ferrous/ferric anode reaction at a total cell voltage of less than about 1.5 V and at current densities of greater than about 26 Amps per square foot (about 280 A/m2), and reduces acid mist generation. Furthermore, the use of such a system permits the use of low ferrous iron concentrations and optimized electrolyte flow rates as compared to prior art systems while producing high quality, commercially saleable product (i.e., LME Grade A copper cathode or equivalent), which is advantageous.

A method of designing the layout of an integrated circuit (IC) by deriving an analytical constraint for a cut-based placement partitioner using analytical optimization, and placing cells on the IC with the cut-based placement partitioner using the analytical constraint. Quadratic optimization may be used to determine a desired ratio of a cell area of a given partition to a total cell area (the balance parameter), and placing may be performed using multilevel bisection partitioning constrained by the balance parameter. This implementation may include a determination of an aspect ratio for an entire partitioning region of the integrated circuit, and a "center-of-mass" coordinate of the cells based on the quadratic optimization, which are then used to define a placement rectangle having the same aspect ratio, and centered on the center-of-mass coordinate. This placement rectangle is used to derive the balance parameter. The placement rectangle has a total area equal to a total moveable cell area, and the balance parameter is computed by calculating the ratio of a left portion of the placement rectangle which lies in the left partition to the total area of the placement rectangle. The multilevel partitioner then places a proportionate number of the cells in the left partition based on the balance parameter.

The invention discloses a method for simultaneously inducing and amplifying V alpha<24+>iNKT cells and CD<3->CD<56+>NK cells. The method comprises the steps as follows: PBMC (peripheral blood mononuclear cells) are separated from peripheral blood, the concentration of the PBMC is adjusted to 2*10<6>/ml by a serum-free medium containing autologous plasma; an Anti-CD<16> antibody, -GalCer, IL-2, IL-18 and IL-21 are added, and then the mixture is transferred into a culture flask for culture; an Anti-CD3 antibody is added in a cell suspension in 24 hours; a serum-free medium containing IL-2, IL-18 and IL-21 is supplemented every two days according to the cell growth condition; the cell concentration is controlled to be 1.5*10<6>/ml; and after continuous culture is performed for 14-21 days, large quantities of high-purity V alpha<24+>iNKT cells and CD<3->CD<56+>NK cells can be obtained simultaneously, and the total cell quantity can reach an effective value of the cell quantity required for adoptive cellular immunotherapy clinically for tumor. The method for simultaneous and efficient amplification of the V alpha<24+>iNKT cells and the CD<3->CD<56+>NK cells is simple, convenient and effective.

The invention relates to an extracorporal building method of a tumor microenvironment and an application of the method. By using the method, a three-dimensional stent similar with a cell internal microenvironment is built and total cells of tumor tissues are inoculated to promote recovery of physiological activity of all cells, so that a tumor microenvironment is built for drug screening for individual-based tumor treatment.

The invention belongs to the field of cell culture, particularly relates to a method for in-vitro culture and enrichment of CD8+ T cells, and more particularly relates to a method for directional amplification of a large amount of CD8+ T cells in peripheral blood mononuclear cells of a human body by using an irritant. The T cells obtained by the technical scheme are large in proliferation number and high in activity; the ratio of the CD8+ T cells to CD4+ T cells can be increased to 2-5 : 1 from about 1 : 2 in a normal physiological status; and in a target culture, the content of central memory T cells exceeds 30% based on the total cell number.

The invention discloses a swine in-vitro embryo culture solution. The swine in-vitro embryo culture solution disclosed by the invention is obtained through adding ligustrazine, with the final concentration of 0.05-5.00 microgram/milliliter, into an embryo culture solution NCSU-23. A traditional Chinese medicine monomer component, namely ligustrazine, is added into the swine in-vitro embryo culture solution disclosed by the invention; shown by experiments, through carrying out in-vitro culture on the swine parthenogenetic activated embryos and the swine in-vitro fertilized embryos by using the culture solution, the development efficiency of the embryos and the number of the cell masses and total cells in blastulae can be effectively increased. The swine in-vitro embryo culture solution has the advantages that a foundation is laid for the development of other biotechnologies, medical research and animal husbandry, and meanwhile, a certain theoretical basis for clarifying the development mechanism of early swine embryos is provided.

The invention relates to a method for carrying out automatic classified counting on cells in human blood, which comprises the following steps: (1) generating a two-dimensional scatter diagram on two one-dimensional data; (2) carrying out smooth treatment on the two-dimensional scatter diagram so as to make points at the border of the class and the main body part of the class continuous, and eliminating distant discrete points; (3) clustering and searching a class center by taking the two-dimensional scatter diagram after smooth treatment as a foundation, and classifying and labeling the cells; (4) counting the cells in the positions with the same label, and calculating the percentage of each class of cells accounting for the total cells. The method disclosed by the invention can be used for carrying out adaptive classification based on data characteristics, the border of each class can be automatically adjusted according to the characteristics of the scatter diagram of a blood sample, the defects that fixed classification cannot be adjusted according to blood characteristics and the efficiency of manual border adjustment is low are overcome, and the method can be used in an automatic blood cell analyzer for rapid analysis.

The present invention is perfusion culture medicine method and apparatus for hematopoietic cell. The apparatus includes at least stirring bioreactor and perfusing culture cavity connected via pipeline to the stirring bioreactor. The perfusion culture process includes first static perfusion culture until reaching higher cell density, and subsequent stirring suspension culture to eliminate the cell density gradient and accelerate cell growth. Test result shows that the initial culture period has cloning in great amount of total cell as well as CFU-Mix, CFU-GM and CD34+ cell, and the subsequent stirring suspension culture stage has further cloning of total cell as well as CFU-Mix, CFU-GM and CD34+ cell. The said method has greater cloning than single stirring suspension culture.

The invention provides a method for inducing bone mesenchymal stem cells to differentiate into osteoblast. The method is characterized in that little osteoblast is added in a bone mesenchymal stem cell culture system to form an osteogenesis micro-environment; the quantity of the osteoblast is more than 30% of the total cell quantity preferably. The method firstly uses the osteogenesis micro-environment provided by little osteoblast, induces the BMSCs to form mature bone tissue in vitro by a co-culture method and has a wide prospect in the field of bone tissue restoration and reconstruction.