19 results about "Total cell" patented technology

The invention provides a battery diagnosis apparatus and a battery diagnosis method. The battery diagnosis apparatus includes: a data obtaining unit configured to obtain a first target full battery cell curve representing a correspondence relationship between a voltage and a capacity factor of a target cell while a first electrical stimulus is applied to the target cell; and a control circuit configured to generate an estimated total cell curve based on the first target total cell curve and the overpotential curve. The control circuit determines a first set of performance factors as a primary estimate of charge / discharge performance of the target cell by applying the cell diagnostic logic to the estimated full battery curve. The control circuit determines a second set of performance factors as a secondary estimate of charge / discharge performance of the target cell by applying a factor correction model to the first set of performance factors. The second performance factor group comprises an estimation result of the negative pole load capacity of the target single body.

Systems and methods provide a total cell capacity estimation process that accounts for variability of spectral efficiency (SE) within a cell and enables optimized cell adjustments. A network device obtains field data of user equipment (UE) devices for a cell and assigns, based on the field data, multiple zones within the cell. Each of the multiple zones corresponds to a different link adaptation setting for UE devices. The network device computes a total data transfer capacity in a band for each zone of the multiple zones, and estimates a current available remaining data transfer capacity for each zone of the multiple zones based on the field data and the total data transfer capacity for each zone.

Embodiments of the present application provide a kind of detection method, device, electronic equipment and storage medium of the sampling harness of power battery, wherein the method comprises: obtaining the total cell string number and cell voltage of battery module;Extract the cell string number corresponding to the cell voltage;Determine whether the cell string number is less than or equal to the total cell string number;If yes, obtain the first on-off information of the sampling harness according to the cell string number and the total cell string number;If not, obtain the second on-off information of the sampling harness. By implementing the embodiments of the present application, the fault of the sampling harness can be accurately detected, the pin error of the sampling harness is prevented, and the detection efficiency is improved.

The invention provides a battery diagnosis apparatus and a battery diagnosis method. The battery diagnosis apparatus includes: a data obtaining unit configured to obtain a first target full battery cell curve representing a correspondence relationship between a voltage and a capacity factor of a target cell and impedance information of the target cell while a first electrical stimulus is applied to the target cell; and a control circuit configured to generate an estimated total cell curve based on the first target total cell curve and the overpotential curve. The control circuit determines a first set of performance factors as a primary estimate of charge / discharge performance of the target cell by applying the cell diagnostic logic to the estimated full battery curve. The control circuit determines a second set of performance factors as a secondary estimate of charge / discharge performance of the target cell by applying a factor correction model to the first set of performance factors and the impedance information.

Provided in the present application are a communication method and a communication apparatus. The method comprises: receiving first indication information, which indicates one or more of the following: whether KS reference signal resources comprise high-priority reference signal resources, the number of high-priority reference signal resources being M(1)R(2), and M(3)R(4) high-priority reference signal resources, wherein K(5)S(6) is an integer greater than or equal to 1, and M(7)R(8) is an integer greater than or equal to 0; by means of K(9)S(10) reference signal resources, receiving reference signals, wherein the K(11)S(12) reference signal resources comprise M(13)R(14) high-priority reference signal resources, and obtaining M pieces of channel state information, wherein M is an integer greater than or equal to 1; and sending the M pieces of channel state information. In this way, comprehensive consideration of the scheduling requirements of other terminal devices in a network can be realized, and assistance is provided for a terminal device selecting appropriate reference signal resources for channel state information measurement and reporting, thereby improving the total cell capacity.

The present application provides a communication method and a communication apparatus. The method comprises: a terminal device receiving first indication information, the first indication information indicating the priorities of N reference signal resources, and N being an integer greater than 1; receiving a reference signal by means of the N reference signal resources; on the basis of the priorities of the N reference signal resources, measuring at least one reference signal resource among the N reference signal resources to obtain M pieces of channel state information, wherein M is an integer greater than or equal to 1; sending the M pieces of channel state information. In this way, the scheduling requirements of other terminal devices in a network can be comprehensively considered, the terminal devices are assisted in selection of channel state information of proper reference signal resources for measurement and reporting, and the total cell capacity of is improved.

The invention discloses trophoblasts for amplification of clinical-grade tumor infiltrating lymphocytes and a preparation method and application of the trophoblasts, and the preparation method comprises the following steps: 1) placing tumor tissues in a complete culture medium, placing the complete culture medium in a 5% CO2 incubator at 37 DEG C, and carrying out wet culture for 2-3 weeks; after finishing, sorting the lymphocytes by magnetic beads; (2) adding the lymphocytes into an amplification culture medium for culturing, transferring the lymphocytes into a cell culture bag after the total number of the cells is greater than 107-108, culturing until the total number of the cells reaches 109-1011, and centrifugally collecting the cells to obtain tumor infiltrating lymphocytes TIL; 3, 10-50 Gy X rays or gamma rays are used for irradiating the tumor infiltrating lymphocytes to damage DNA of the tumor infiltrating lymphocytes to prevent cell division, and the trophoblasts are obtained.According to the method, a large number of amplified TIL is used for replacing PBMC to serve as the trophoblasts for immune cell proliferation, and the lymphocyte trophoblasts which are easier to obtain and better in nourishing effect are provided.

The application provides a content extraction method based on cell membrane capacitance change, and belongs to the field of cell-level micromanipulation technology, and comprises the following steps: S1, a change relationship between cell membrane capacitance and cell membrane surface area is established; S2, a change relationship between cell membrane surface area and volume is approximately established; S3, high-resistance sealing and membrane rupture of cells are completed, constant voltage cell content extraction operation is performed, and cell membrane capacitance values in the extraction process are obtained in real time through capacitance fitting; S4, content extraction amount is estimated; S5, actual cytoplasm extraction amount is calculated based on a rotary L-shaped electrode, the relationship model established in S2 is corrected, and finally, quantitative extraction of cell contents is realized. The application utilizes an electrical model of cell membrane capacitance, realizes real-time feedback of cell content extraction amount, realizes quantitative control of content extraction, can realize a cytoplasm extraction success rate of 62.5% in the case of a whole cell patch clamp structure, and the average cytoplasm extraction amount control error is less than 6% of the total cell volume.

The invention provides a battery diagnosis apparatus and a battery diagnosis method. The battery diagnosis apparatus includes: a data obtaining unit configured to obtain a first target full battery cell curve representing a correspondence relationship between a voltage and a capacity factor of a target cell while a first electrical stimulus is applied to the target cell; and a control circuit configured to generate an estimated total cell curve based on the first target total cell curve and the overpotential curve. The control circuit determines a first set of performance factors as a primary estimate of charge / discharge performance of the target cell by applying the cell diagnostic logic to the estimated full battery curve. The control circuit determines a second set of performance factors as a secondary estimate of charge / discharge performance of the target cell by applying a factor correction model to the first set of performance factors. The second performance factor group comprises an estimation result of the negative pole scaling factor of the target cell.

A sequence of microscopic images, each comprising an array of pixels, visualizing fluorescently labeled cellular components within live cells is received. These images are segmented to identify one or more cells visualized within the images. A machine learning model assigns a cell health score and / or cell health subtype score to each pixel of a particular cell. The cell health scores and / or cell health subtype scores based on these pixels are used to calculate a total cell health score and / or total cell health subtype score for each cell. Data characterizing the calculated scores is provided to a consuming application or process.

The application discloses a culture solution for improving the blastocyst rate and quality of bovine embryos, application and an in-vitro culture method, and relates to the technical field of animal embryo engineering. The application provides a culture solution for in-vitro culture of bovine embryos, which comprises a first culture solution and a second culture solution with different osmotic pressure concentrations. By controlling the osmotic pressure concentrations of the first culture solution and the second culture solution in a suitable range, and by using the first culture solution in the early stage of embryo development and the second culture solution in the late stage of embryo development, the in-vitro culture system can better simulate the environment in the oviduct in the early stage of embryo development and better simulate the environment in the uterus in the late stage of embryo development, so that the osmotic pressure stress of the embryo in the development process is reduced, and the blastocyst rate and the blastocyst quality of the bovine embryo are improved. Compared with a traditional culture system with constant osmotic pressure, the application significantly improves the blastocyst rate by sequential regulation of the osmotic pressure, the total cell number of the obtained blastocysts is more, and the apoptosis index is lower.

Provided herein are methods for using particle counting to determine live cell concentration, total cell concentration, cell viability and biomass of cell aggregate compositions, such as for accurate cell dosing for clinical applications.

The present disclosure relates to a method for determining cell viability in a population comprising cell aggregates. The methods typically comprise contacting a sample prepared from a cell population with a first agent and a second agent, and determining the ratio of living cells to total cells, e.g., following three-dimensional imaging of cell aggregates contacted with the two agents.

The invention provides a battery diagnosis apparatus and a battery diagnosis method. The battery diagnosis apparatus includes: a data obtaining unit configured to obtain a first target total battery cell curve representing a correspondence relationship between a voltage and a capacity factor of a target cell and temperature information of the target cell while a first electrical stimulus is applied to the target cell; and a control circuit configured to generate an estimated total cell curve based on the first target total cell curve and the overpotential curve. The control circuit determines a first set of performance factors as a primary estimate of charge / discharge performance of the target cell by applying the cell diagnostic logic to the estimated full battery curve. The control circuit determines a second set of performance factors as a secondary estimate of charge / discharge performance of the target cell by applying a factor correction model to the first set of performance factors and the temperature information.

The invention relates to the technical field of biology, in particular to a myocardial cell in-vitro amplification and passage method which comprises the following steps: S1, culturing human induced pluripotent stem cells until the cell confluence degree reaches 70%-80%; s2, differentiating the human induced pluripotent stem cells into myocardial cells; s3, observing the number of pulsated myocardial cells, selecting holes with the pulsation percentage of 50%-90%, digesting and re-suspending the myocardial cells in the holes, counting the cells, laying the cells into a six-hole plate according to 1 / 20-1 / 10 of the total cell number, and culturing for 24 hours by using an RPMI / B-27 insulin-free culture medium containing Y-27632; wherein the concentration of the Y-27632 is 2 to 10 [mu] M; s4, replacing the culture medium with an RPMI / B-27 insulin-free culture medium containing Chir99021, replacing the culture medium every day, culturing for 3-5 days until the cell density is 70%-80%, and arranging next passage and amplification; s5, the steps S3 and S4 are repeated according to needs for amplification and passage, the cardiac muscle cells with the increased number are obtained, and the problem that large-scale preparation is limited during in-vitro amplification of the human pluripotent stem cell-derived cardiac muscle cells is solved.

Provided in the embodiments of the present disclosure is a method for evaluating electrical losses in a photovoltaic array. The method comprises: on the basis of an uncovered cell surface total radiation amount, a cell surface total radiation amount in a covered region, a cell surface covered area and a total cell area, calculating a cell surface equivalent total radiation amount; on the basis of the cell surface equivalent total radiation amount and a cell covered equivalent electric current condition, calculating a cell covered electric current, and on the basis of the uncovered cell surface total radiation amount and a cell uncovered equivalent electric current condition, calculating a cell uncovered electric current; on the basis of a covered power generation amount, an uncovered power generation amount and a total covering loss condition, calculating a total covering loss; on the basis of a total covered area, a covered radiation amount, an array area and an uncovered radiation amount of a photovoltaic array, calculating a theoretical radiation loss per unit of time of the photovoltaic array; using the uncovered radiation amount within a preset time period as a weight to perform weighted calculation on a weighted radiation loss of the photovoltaic array within the preset time period; and on the basis of the total covering loss and the weighted radiation loss of the photovoltaic array, calculating the difference to calculate the electrical loss ratio of the photovoltaic array.

Provided herein are methods for using particle counting to determine live cell concentration, total cell concentration, cell viability and biomass of cell aggregate compositions, such as for accurate cell dosing for clinical applications.

The embodiment of the invention provides a cell activity detection method based on cell image analysis and a related device. The method comprises the following steps: acquiring a target cell image; performing batch automatic segmentation on the target cell image by using a Cellpose platform to generate a segmented mask image; inputting the target cell image and the segmented mask image into an illic platform for classification, and determining the number of normal cells and the number of abnormal cells in the target cell image; and obtaining a cell activity detection result corresponding to the target cell image according to the ratio of the number of the abnormal cells to the total cell number, the total cell number being the sum of the number of the normal cells and the number of the abnormal cells. Based on this, the embodiment of the invention does not need to depend on labeling training on a large amount of data, adopts a simple image analysis means, can efficiently and accurately evaluate the cell activity by calculating the ratio of the number of abnormal cells to the number of total cells, and has the advantages of low cost, rapidness and high efficiency.

The present invention addresses the problem of providing: a method for producing a three-dimensional cell culture comprising human-derived nerve cells, astrocytes, and microglial cells capable of simulating the function of the human brain; and a method for culturing the three-dimensional cell culture. Comprising nerve cells, astrocytes and microglial cells derived from human beings and capable of simulating human brain functions; and a method for evaluating a substance to be measured using the three-dimensional cell culture. According to the present invention, provided is a method for producing a three-dimensional cell culture comprising a step for adding human-derived astrocytes, human-derived nerve cells, and human-derived microglial cells to a culture vessel prior to co-culture of cells; and co-culturing the astrocytes, the nerve cells, and the microglial cells in a culture vessel, in which the astrocytes account for 30% or more of the total cells added to the culture vessel.