8 results about "Total cell" patented technology

Systems and methods provide a total cell capacity estimation process that accounts for variability of spectral efficiency (SE) within a cell and enables optimized cell adjustments. A network device obtains field data of user equipment (UE) devices for a cell and assigns, based on the field data, multiple zones within the cell. Each of the multiple zones corresponds to a different link adaptation setting for UE devices. The network device computes a total data transfer capacity in a band for each zone of the multiple zones, and estimates a current available remaining data transfer capacity for each zone of the multiple zones based on the field data and the total data transfer capacity for each zone.

Embodiments of the present application provide a kind of detection method, device, electronic equipment and storage medium of the sampling harness of power battery, wherein the method comprises: obtaining the total cell string number and cell voltage of battery module;Extract the cell string number corresponding to the cell voltage;Determine whether the cell string number is less than or equal to the total cell string number;If yes, obtain the first on-off information of the sampling harness according to the cell string number and the total cell string number;If not, obtain the second on-off information of the sampling harness. By implementing the embodiments of the present application, the fault of the sampling harness can be accurately detected, the pin error of the sampling harness is prevented, and the detection efficiency is improved.

The invention discloses trophoblasts for amplification of clinical-grade tumor infiltrating lymphocytes and a preparation method and application of the trophoblasts, and the preparation method comprises the following steps: 1) placing tumor tissues in a complete culture medium, placing the complete culture medium in a 5% CO2 incubator at 37 DEG C, and carrying out wet culture for 2-3 weeks; after finishing, sorting the lymphocytes by magnetic beads; (2) adding the lymphocytes into an amplification culture medium for culturing, transferring the lymphocytes into a cell culture bag after the total number of the cells is greater than 107-108, culturing until the total number of the cells reaches 109-1011, and centrifugally collecting the cells to obtain tumor infiltrating lymphocytes TIL; 3, 10-50 Gy X rays or gamma rays are used for irradiating the tumor infiltrating lymphocytes to damage DNA of the tumor infiltrating lymphocytes to prevent cell division, and the trophoblasts are obtained.According to the method, a large number of amplified TIL is used for replacing PBMC to serve as the trophoblasts for immune cell proliferation, and the lymphocyte trophoblasts which are easier to obtain and better in nourishing effect are provided.

The application provides a content extraction method based on cell membrane capacitance change, and belongs to the field of cell-level micromanipulation technology, and comprises the following steps: S1, a change relationship between cell membrane capacitance and cell membrane surface area is established; S2, a change relationship between cell membrane surface area and volume is approximately established; S3, high-resistance sealing and membrane rupture of cells are completed, constant voltage cell content extraction operation is performed, and cell membrane capacitance values in the extraction process are obtained in real time through capacitance fitting; S4, content extraction amount is estimated; S5, actual cytoplasm extraction amount is calculated based on a rotary L-shaped electrode, the relationship model established in S2 is corrected, and finally, quantitative extraction of cell contents is realized. The application utilizes an electrical model of cell membrane capacitance, realizes real-time feedback of cell content extraction amount, realizes quantitative control of content extraction, can realize a cytoplasm extraction success rate of 62.5% in the case of a whole cell patch clamp structure, and the average cytoplasm extraction amount control error is less than 6% of the total cell volume.

A sequence of microscopic images, each comprising an array of pixels, visualizing fluorescently labeled cellular components within live cells is received. These images are segmented to identify one or more cells visualized within the images. A machine learning model assigns a cell health score and / or cell health subtype score to each pixel of a particular cell. The cell health scores and / or cell health subtype scores based on these pixels are used to calculate a total cell health score and / or total cell health subtype score for each cell. Data characterizing the calculated scores is provided to a consuming application or process.

The application discloses a culture solution for improving the blastocyst rate and quality of bovine embryos, application and an in-vitro culture method, and relates to the technical field of animal embryo engineering. The application provides a culture solution for in-vitro culture of bovine embryos, which comprises a first culture solution and a second culture solution with different osmotic pressure concentrations. By controlling the osmotic pressure concentrations of the first culture solution and the second culture solution in a suitable range, and by using the first culture solution in the early stage of embryo development and the second culture solution in the late stage of embryo development, the in-vitro culture system can better simulate the environment in the oviduct in the early stage of embryo development and better simulate the environment in the uterus in the late stage of embryo development, so that the osmotic pressure stress of the embryo in the development process is reduced, and the blastocyst rate and the blastocyst quality of the bovine embryo are improved. Compared with a traditional culture system with constant osmotic pressure, the application significantly improves the blastocyst rate by sequential regulation of the osmotic pressure, the total cell number of the obtained blastocysts is more, and the apoptosis index is lower.

The invention relates to the technical field of biology, in particular to a myocardial cell in-vitro amplification and passage method which comprises the following steps: S1, culturing human induced pluripotent stem cells until the cell confluence degree reaches 70%-80%; s2, differentiating the human induced pluripotent stem cells into myocardial cells; s3, observing the number of pulsated myocardial cells, selecting holes with the pulsation percentage of 50%-90%, digesting and re-suspending the myocardial cells in the holes, counting the cells, laying the cells into a six-hole plate according to 1 / 20-1 / 10 of the total cell number, and culturing for 24 hours by using an RPMI / B-27 insulin-free culture medium containing Y-27632; wherein the concentration of the Y-27632 is 2 to 10 [mu] M; s4, replacing the culture medium with an RPMI / B-27 insulin-free culture medium containing Chir99021, replacing the culture medium every day, culturing for 3-5 days until the cell density is 70%-80%, and arranging next passage and amplification; s5, the steps S3 and S4 are repeated according to needs for amplification and passage, the cardiac muscle cells with the increased number are obtained, and the problem that large-scale preparation is limited during in-vitro amplification of the human pluripotent stem cell-derived cardiac muscle cells is solved.